TY  - JOUR
AU  - Bacsur, Péter
AU  - Rutka, Mariann
AU  - Asbóth, András
AU  - Resál, Tamás
AU  - Szántó, Kata Judit
AU  - Jójárt, Boldizsár
AU  - Bálint, Anita
AU  - Ari, Eszter
AU  - Ajibola, Walliyulahi
AU  - Kintses, Bálint
AU  - Fehér, Tamás
AU  - Pigniczki , Daniella
AU  - Bor, Renáta
AU  - Fábián, Anna
AU  - Maléth, József
AU  - Szepes, Zoltán
AU  - Farkas, Klaudia
AU  - Molnár, Tamás
TI  - Effects of bowel cleansing on the composition of the gut microbiota in inflammatory bowel disease patients and healthy controls
JF  - THERAPEUTIC ADVANCES IN GASTROENTEROLOGY
J2  - THER ADV GASTROENTER
VL  - 16
PY  - 2023
PG  - 13
SN  - 1756-283X
DO  - 10.1177/17562848231174298
UR  - https://m2.mtmt.hu/api/publication/34014839
ID  - 34014839
N1  - "Asbóth Andrásnál tévesen szerepel a publikáción az SZTE/TTIK/BI/Biokémiai és Molekuláris Biológiai Tanszék. (SE, SZTE admin5)"
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Apjok, Gábor
AU  - Számel, Mónika
AU  - Christodoulou, Chryso
AU  - Seregi, Viktória
AU  - Vásárhelyi, Bálint Márk
AU  - Stirling, Tamás
AU  - Eszenyi, Bálint Dénes
AU  - Sári , Tóbiás
AU  - Vidovics, Fanni
AU  - Nagrand, Erika
AU  - Kovács, Dorina
AU  - Szili, Petra
AU  - Lantos, Ildikó Ilona
AU  - Méhi, Orsolya Katinka
AU  - Jangir, Pramod Kumar
AU  - Herczeg, Róbert
AU  - Gálik, Bence
AU  - Urbán, Péter
AU  - Gyenesei, Attila
AU  - Draskovits, Gábor
AU  - Nyerges, Ákos
AU  - Fekete, Gergely
AU  - Bodai, László
AU  - Zsindely, Nóra
AU  - Dénes, Béla
AU  - Yosef, Ido
AU  - Qimron, Udi
AU  - Papp, Balázs
AU  - Pál, Csaba
AU  - Kintses, Bálint
TI  - Characterization of antibiotic resistomes by reprogrammed bacteriophage-enabled functional metagenomics in clinical strains
JF  - NATURE MICROBIOLOGY
J2  - NAT MICROBIOL
VL  - 8
PY  - 2023
IS  - 3
SP  - 410
EP  - 423
PG  - 14
SN  - 2058-5276
DO  - 10.1038/s41564-023-01320-2
UR  - https://m2.mtmt.hu/api/publication/33634821
ID  - 33634821
N1  - Funding Agency and Grant Number: National Laboratory of Biotechnology Grants [NKFIH-871-3/2020, 2022-2.1.1-NL-2022-00008]; European Union [754432]; European Research Council [648364, 862077]; National Research, Development and Innovation Office grant [FK-135245, FK-124254]; National Research, Development and Innovation Office; Ministry for Innovation and Technology [KKP 129814, 126506]; New National Excellence Program of the Ministry of Human Capacities [UNKP-20-5-SZTE-654, UNKP-21-5-SZTE-579]; New National Excellence Program of the Ministry for Innovation and Technology - National Research, Development and Innovation Fund [UNKP-20-3 -SZTE-452]; Doctoral Student Scholarship Program of the Co-Operative Doctoral Program of the Ministry of Innovation and Technology - National Research, Development and Innovation Fund [KDP-17-4/ PALY-2021, C992025]; European Union's Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant [754432]; Polish Ministry of Science and Higher Education; National Academy of Scientist Education Program of the National Biomedical Foundation under Hungarian Ministry of Culture and Innovation; National Laboratory for Health Security [RRF-2.3.1-212022-00006, GINOP-2.3.2-15-2016-00014, GINOP-2.3.2-15-2016-00020, GINOP-2.3.2-15-2016-00035]; Janos Bolyai Research Fellowship from the Hungarian Academy of Sciences [BO/352/20, BO/00303/19/8];  [GINOP-2.3.4-15-2020-00010];  [GINOP-2.3.1-20-2020-00001];  [BECOMING-2019-1-HU01-KA203-061251]
            Funding text: We thank D. Verma from the Department of Microbiology and B. Bhimrao of Ambedkar University, Lucknow, India for help with soil sample collection and NBA approval. This work was supported by National Laboratory of Biotechnology Grants NKFIH-871-3/2020 and 2022-2.1.1-NL-2022-00008 (B.K. and C.P.); the European Union's Horizon 2020 research and innovation programme under grant agreement no. 739593 (B.P. and B.K.); the European Research Council H2020-ERC-2014-CoG 648364-Resistance Evolution (C.P.) and H2020-ERC-2019-PoC 862077-Aware (C.P.); National Research, Development and Innovation Office grant FK-135245 (B.K.) and FK-124254 (O.M.); the National Research, Development and Innovation Office and the Ministry for Innovation and Technology under the `Frontline' Programme KKP 129814 and 126506 (B.P. and C.P.); the National Laboratory for Health Security RRF-2.3.1-212022-00006 (B.P.), GINOP-2.3.2-15-2016-00014 (EVOMER, C.P. and B.P.), GINOP-2.3.2-15-2016-00020 (MolMedEx TUMORDNS, C.P.), GINOP-2.3.2-15-2016-00035 (N.Z.); a Janos Bolyai Research Fellowship from the Hungarian Academy of Sciences (BO/352/20 (B.K.), BO/00303/19/8 (O.M)); New National Excellence Program of the Ministry of Human Capacities (UNKP-20-5-SZTE-654 and UNKP-215-SZTE-579, B.K.); New National Excellence Program of the Ministry for Innovation and Technology funded by the National Research, Development and Innovation Fund (UNKP-20-3 -SZTE-452, G.A.); the Doctoral Student Scholarship Program of the Co-Operative Doctoral Program of the Ministry of Innovation and Technology financed by the National Research, Development and Innovation Fund (KDP-17-4/ PALY-2021, C992025, M.S.). R.H., B.G., P.U. and A.G. were supported by GINOP-2.3.4-15-2020-00010, GINOP-2.3.1-20-2020-00001, BECOMING-2019-1-HU01-KA203-061251, the European Union's Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant agreement no. 754432 and the Polish Ministry of Science and Higher Education, from the financial resources for science in 2018-2023. This research work was conducted with the support of the National Academy of Scientist Education Program of the National Biomedical Foundation under the sponsorship of the Hungarian Ministry of Culture and Innovation (D.K.).
AB  - Functional metagenomics is a powerful experimental tool to identify antibiotic resistance genes (ARGs) in the environment, but the range of suitable host bacterial species is limited. This limitation affects both the scope of the identified ARGs and the interpretation of their clinical relevance. Here we present a functional metagenomics pipeline called Reprogrammed Bacteriophage Particle Assisted Multi-species Functional Metagenomics (DEEPMINE). This approach combines and improves the use of T7 bacteriophage with exchanged tail fibres and targeted mutagenesis to expand phage host-specificity and efficiency for functional metagenomics. These modified phage particles were used to introduce large metagenomic plasmid libraries into clinically relevant bacterial pathogens. By screening for ARGs in soil and gut microbiomes and clinical genomes against 13 antibiotics, we demonstrate that this approach substantially expands the list of identified ARGs. Many ARGs have species-specific effects on resistance; they provide a high level of resistance in one bacterial species but yield very limited resistance in a related species. Finally, we identified mobile ARGs against antibiotics that are currently under clinical development or have recently been approved. Overall, DEEPMINE expands the functional metagenomics toolbox for studying microbial communities.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Ari, Eszter
AU  - Vásárhelyi, Bálint Márk
AU  - Kemenesi, Gábor
AU  - Tóth, Gábor Endre
AU  - Zana, Brigitta
AU  - Somogyi, Balázs Antal
AU  - Lanszki, Zsófia
AU  - Röst, Gergely
AU  - Jakab, Ferenc
AU  - Papp, Balázs
AU  - Kintses, Bálint
TI  - A Single Early Introduction Governed Viral Diversity in the Second Wave of SARS-CoV-2 Epidemic in Hungary
JF  - VIRUS EVOLUTION
J2  - VIRUS EVOL
VL  - 8
PY  - 2022
IS  - 2
PG  - 12
SN  - 2057-1577
DO  - 10.1093/ve/veac069
UR  - https://m2.mtmt.hu/api/publication/33040340
ID  - 33040340
AB  - Retrospective evaluation of past waves of the SARS-CoV-2 epidemic is key for designing optimal interventions against future waves and novel pandemics. Here we report on analysing genome sequences of SARS-CoV-2 from the first two waves of the epidemic in 2020 in Hungary, mirroring a suppression and a mitigation strategy, respectively. Our analysis reveals that the two waves markedly differed in viral diversity and transmission patterns. Specifically, unlike in several European areas or in the USA, we have found no evidence for early introduction and cryptic transmission of the virus in the first wave of the pandemic in Hungary. Despite the introduction of multiple viral lineages, extensive community spread was prevented by a timely national lockdown in March 2020. In sharp contrast, the majority of the cases in the much larger second wave can be linked to a single transmission lineage of the pan-European B.1.160 variant. This lineage was introduced unexpectedly early, followed by a two-month-long cryptic transmission before a soar of detected cases in September 2020. Epidemic analysis has revealed that the dominance of this lineage in the second wave was not associated with an intrinsic transmission advantage. This finding is further supported by the rapid replacement of B.1.160 by the alpha variant (B.1.1.7) that launched the third wave of the epidemic in February 2021. Overall, these results illustrate how the founder effect in combination with cryptic transmission, instead of repeated international introductions or higher transmissibility, can govern viral diversity.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Györkei, Ádám
AU  - Daruka, Lejla
AU  - Balogh, Dávid
AU  - Őszi, Erika
AU  - Magyar, Zoltán
AU  - Szappanos, Balázs
AU  - Fekete, Gergely
AU  - Fuxreiter, Mónika
AU  - Horváth, Péter
AU  - Pál, Csaba
AU  - Kintses, Bálint
AU  - Papp, Balázs
TI  - Proteome-wide landscape of solubility limits in a bacterial cell
JF  - SCIENTIFIC REPORTS
J2  - SCI REP
VL  - 12
PY  - 2022
IS  - 1
PG  - 13
SN  - 2045-2322
DO  - 10.1038/s41598-022-10427-1
UR  - https://m2.mtmt.hu/api/publication/32813213
ID  - 32813213
N1  - Funding Agency and Grant Number: ELKH Biological Research Center
            Funding text: Open access funding provided by ELKH Biological Research Center.
AB  - Proteins are prone to aggregate when expressed above their solubility limits. Aggregation may occur rapidly, potentially as early as proteins emerge from the ribosome, or slowly, following synthesis. However, in vivo data on aggregation rates are scarce. Here, we classified the Escherichia coli proteome into rapidly and slowly aggregating proteins using an in vivo image-based screen coupled with machine learning. We find that the majority (70%) of cytosolic proteins that become insoluble upon overexpression have relatively low rates of aggregation and are unlikely to aggregate co-translationally. Remarkably, such proteins exhibit higher folding rates compared to rapidly aggregating proteins, potentially implying that they aggregate after reaching their folded states. Furthermore, we find that a substantial fraction (similar to 35%) of the proteome remain soluble at concentrations much higher than those found naturally, indicating a large margin of safety to tolerate gene expression changes. We show that high disorder content and low surface stickiness are major determinants of high solubility and are favored in abundant bacterial proteins. Overall, our study provides a global view of aggregation rates and hence solubility limits of proteins in a bacterial cell.
LA  - English
DB  - MTMT
ER  - 

TY  - GEN
AU  - Visnyovszki, Ádám
AU  - Orosz, László
AU  - Kintses, Bálint
AU  - Stirling, Tamás
AU  - Vásárhelyi, Bálint Márk
AU  - Ari, Eszter
AU  - Kiss, Enikő
AU  - Papp, Balázs
AU  - Apjok, Gábor
AU  - Vidovics, Fanni
AU  - Lakatos, Lóránt
AU  - Lengyel, György
AU  - Ánosi, Noel
AU  - Sóki, József
AU  - Baaity, Zain
AU  - Hajdú, Edit
AU  - Burián, Katalin
TI  - A COVID-19 PANDÉMIÁHOZ TÁRSULÓAN ELŐFORDULT MULTIREZISZTENS ACINETOBACTER BAUMANNII TÖRZSEK MOLEKULÁRIS JELLEMZÉSE
PY  - 2021
UR  - https://m2.mtmt.hu/api/publication/32476999
ID  - 32476999
LA  - Hungarian
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Kovács, Károly
AU  - Farkas, Zoltán
AU  - Bajić, Djordje
AU  - Kalapis, Dorottya
AU  - Daraba, Andreea
AU  - Almási, Karola
AU  - Kintses, Bálint
AU  - Bódi, Zoltán
AU  - Notebaart, Richard A
AU  - Poyatos, Juan F
AU  - Kemmeren, Patrick
AU  - Holstege, Frank C P
AU  - Pál, Csaba
AU  - Papp, Balázs
TI  - Suboptimal global transcriptional response increases the harmful effects of loss-of-function mutations
JF  - MOLECULAR BIOLOGY AND EVOLUTION
J2  - MOL BIOL EVOL
VL  - 38
PY  - 2021
IS  - 3
SP  - 1137
EP  - 1150
PG  - 14
SN  - 0737-4038
DO  - 10.1093/molbev/msaa280
UR  - https://m2.mtmt.hu/api/publication/31720772
ID  - 31720772
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Bálint, Anita
AU  - Farkas, Klaudia
AU  - Méhi, Orsolya Katinka
AU  - Kintses, Bálint
AU  - Vásárhelyi, Bálint Márk
AU  - Ari, Eszter
AU  - Pál, Csaba
AU  - Madácsy, Tamara
AU  - Maléth, József
AU  - Szántó, Kata Judit
AU  - Nagy, István
AU  - Rutka, Mariann
AU  - Bacsur, Péter
AU  - Szűcs, Diána
AU  - Szepes, Zoltán
AU  - Nagy, Ferenc
AU  - Fábián, Anna
AU  - Bor, Renáta
AU  - Milassin, Ágnes
AU  - Molnár, Tamás
TI  - Functional Anatomical Changes in Ulcerative Colitis Patients Determine Their Gut Microbiota Composition and Consequently the Possible Treatment Outcome
JF  - PHARMACEUTICALS
J2  - PHARMACEUTICALS-BASE
VL  - 13
PY  - 2020
IS  - 11
PG  - 16
SN  - 1424-8247
DO  - 10.3390/ph13110346
UR  - https://m2.mtmt.hu/api/publication/31647953
ID  - 31647953
N1  - Department of Medicine, University of Szeged, Szeged, 6720, Hungary            
            Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, Szeged, 6726, Hungary            
            Department of Genetics, Eötvös Loránd University, Budapest, 1053, Hungary            
            ‘Momentum’ Epithelial Signalization and Secretion Workgroup, Hungarian Academy of Sciences, Department of Medicine, University of Szeged, Szeged, 1051, Hungary            
            Institute of Biochemistry, Biological Research Centre, Szeged, 6726, Hungary            
            HCEMM-BRC Translational Microbiology Lab, Szeged, 6726, Hungary            
            Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, 6720, Hungary            
            HCEMM-BRC Metabolic Systems Biology Lab, Szeged, 6726, Hungary            
            Cited By :1            
            Export Date: 14 January 2021            
            Correspondence Address: Molnár, T.; Department of Medicine, University of SzegedHungary; email: molnar.tamas@med.u-szeged.hu
Department of Medicine, University of Szeged, Szeged, 6720, Hungary            
            Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, Szeged, 6726, Hungary            
            Department of Genetics, Eötvös Loránd University, Budapest, 1053, Hungary            
            ‘Momentum’ Epithelial Signalization and Secretion Workgroup, Hungarian Academy of Sciences, Department of Medicine, University of Szeged, Szeged, 1051, Hungary            
            Institute of Biochemistry, Biological Research Centre, Szeged, 6726, Hungary            
            HCEMM-BRC Translational Microbiology Lab, Szeged, 6726, Hungary            
            Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, 6720, Hungary            
            HCEMM-BRC Metabolic Systems Biology Lab, Szeged, 6726, Hungary            
            Cited By :1            
            Export Date: 30 July 2021            
            Correspondence Address: Molnár, T.; Department of Medicine, Hungary; email: molnar.tamas@med.u-szeged.hu
Department of Medicine, University of Szeged, Szeged, 6720, Hungary            
            Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, Szeged, 6726, Hungary            
            Department of Genetics, Eötvös Loránd University, Budapest, 1053, Hungary            
            ‘Momentum’ Epithelial Signalization and Secretion Workgroup, Hungarian Academy of Sciences, Department of Medicine, University of Szeged, Szeged, 1051, Hungary            
            Institute of Biochemistry, Biological Research Centre, Szeged, 6726, Hungary            
            HCEMM-BRC Translational Microbiology Lab, Szeged, 6726, Hungary            
            Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, 6720, Hungary            
            HCEMM-BRC Metabolic Systems Biology Lab, Szeged, 6726, Hungary            
            Cited By :1            
            Export Date: 25 August 2021            
            Correspondence Address: Molnár, T.; Department of Medicine, Hungary; email: molnar.tamas@med.u-szeged.hu
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Nyerges, Ákos
AU  - Tomasic, Tihomir
AU  - Durcik, Martina
AU  - Révész, Tamás
AU  - Szili, Petra
AU  - Draskovits, Gábor
AU  - Bogár, Ferenc
AU  - Skok, Ziga
AU  - Zidar, Nace
AU  - Ilas, Janez
AU  - Zega, Anamarija
AU  - Kikelj, Danijel
AU  - Daruka, Lejla
AU  - Kintses, Bálint
AU  - Vásárhelyi, Bálint Márk
AU  - Földesi, Imre
AU  - Kata, Diána
AU  - Welin, Martin
AU  - Kimbung, Raymond
AU  - Focht, Dorota
AU  - Masic, Lucija Peterlin
AU  - Pál, Csaba
TI  - Rational design of balanced dual-targeting antibiotics with limited resistance
JF  - PLOS BIOLOGY
J2  - PLOS BIOL
VL  - 18
PY  - 2020
IS  - 10
PG  - 31
SN  - 1544-9173
DO  - 10.1371/journal.pbio.3000819
UR  - https://m2.mtmt.hu/api/publication/31642956
ID  - 31642956
N1  - Synthetic and Systems Biology Unit, Biological Research Center, Szeged, Hungary            
            University of Ljubljana, Faculty of Pharmacy, Ljubljana, Slovenia            
            Doctoral School of Theoretical Medicine, University of Szeged, Szeged, Hungary            
            Doctoral School of Multidisciplinary Medical Sciences, University of Szeged, Szeged, Hungary            
            MTA-SZTE Biomimetic Systems Research Group, Department of Medical Chemistry, University of Szeged, Hungary            
            Doctoral School of Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary            
            HCEMM-BRC Translational Microbiology Lab, Szeged, Hungary            
            Department of Laboratory Medicine, University of Szeged, Szeged, Hungary            
            SARomics Biostructures, Medicon Village, Lund, Sweden            
            Export Date: 8 December 2020            
            CODEN: PBLIB            
            Correspondence Address: Mašič, L.P.; University of Ljubljana, Faculty of Pharmacy, Synthetic and Systems Biology Unit, Biological Research CenterSlovenia; email: lucija.peterlin@ffa.uni-lj.si
Synthetic and Systems Biology Unit, Biological Research Center, Szeged, Hungary            
            University of Ljubljana, Faculty of Pharmacy, Ljubljana, Slovenia            
            Doctoral School of Theoretical Medicine, University of Szeged, Szeged, Hungary            
            Doctoral School of Multidisciplinary Medical Sciences, University of Szeged, Szeged, Hungary            
            MTA-SZTE Biomimetic Systems Research Group, Department of Medical Chemistry, University of Szeged, Hungary            
            Doctoral School of Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary            
            HCEMM-BRC Translational Microbiology Lab, Szeged, Hungary            
            Department of Laboratory Medicine, University of Szeged, Szeged, Hungary            
            SARomics Biostructures, Medicon Village, Lund, Sweden            
            Cited By :2            
            Export Date: 22 April 2021            
            CODEN: PBLIB            
            Correspondence Address: Mašič, L.P.; University of Ljubljana, Slovenia; email: lucija.peterlin@ffa.uni-lj.si            
            Correspondence Address: Pal, C.; Synthetic and Systems Biology Unit, Hungary; email: cpal@brc.hu
AB  - Antibiotics that inhibit multiple bacterial targets offer a promising therapeutic strategy against resistance evolution, but developing such antibiotics is challenging. Here we demonstrate that a rational design of balanced multitargeting antibiotics is feasible by using a medicinal chemistry workflow. The resultant lead compounds, ULD1 and ULD2, belonging to a novel chemical class, almost equipotently inhibit bacterial DNA gyrase and topoisomerase IV complexes and interact with multiple evolutionary conserved amino acids in the ATP-binding pockets of their target proteins. ULD1 and ULD2 are excellently potent against a broad range of gram-positive bacteria. Notably, the efficacy of these compounds was tested against a broad panel of multidrug-resistantStaphylococcus aureusclinical strains. Antibiotics with clinical relevance against staphylococcal infections fail to inhibit a significant fraction of these isolates, whereas both ULD1 and ULD2 inhibit all of them (minimum inhibitory concentration [MIC] <= 1 mu g/mL). Resistance mutations against these compounds are rare, have limited impact on compound susceptibility, and substantially reduce bacterial growth. Based on their efficacy and lack of toxicity demonstrated in murine infection models, these compounds could translate into new therapies against multidrug-resistant bacterial infections.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Kintses, Bálint
AU  - Jangir, Pramod Kumar
AU  - Fekete, Gergely
AU  - Számel, Mónika
AU  - Méhi, Orsolya Katinka
AU  - Spohn, Réka
AU  - Daruka, Lejla
AU  - Martins, Ana
AU  - Hosseinnia, A.
AU  - Gagarinova, A.
AU  - Kim, S.
AU  - Phanse, S.
AU  - Csörgő, Bálint
AU  - Györkei, Ádám
AU  - Ari, Eszter
AU  - Lázár, Viktória
AU  - Nagy, István
AU  - Babu, M.
AU  - Pál, Csaba
AU  - Papp, Balázs
TI  - Chemical-genetic profiling reveals limited cross-resistance between antimicrobial peptides with different modes of action
JF  - NATURE COMMUNICATIONS
J2  - NAT COMMUN
VL  - 10
PY  - 2019
IS  - 1
SN  - 2041-1723
DO  - 10.1038/s41467-019-13618-z
UR  - https://m2.mtmt.hu/api/publication/31038930
ID  - 31038930
N1  - Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary            
            HCEMM-BRC Translational Microbiology Lab, Szeged, Hungary            
            Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, Hungary            
            Doctoral School of Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary            
            HCEMM-BRC Metabolic Systems Biology Lab, Szeged, Hungary            
            Department of Biochemistry, University of Regina, Regina, SK, Canada            
            Department of Biochemistry, University of Saskatchewan, Saskatoon, SK, Canada            
            Department of Genetics, Eötvös Loránd University, Budapest, Hungary            
            Sequencing Platform, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary            
            Department of Microbiology and Immunology, University of California, San Francisco, United States            
            Faculty of Biology, Technion – Israel Institute of Technology, Haifa, Israel            
            Export Date: 6 January 2020            
            Correspondence Address: Kintses, B.; Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research CentreHungary; email: kintses.balint@brc.hu
Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary            
            HCEMM-BRC Translational Microbiology Lab, Szeged, Hungary            
            Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, Hungary            
            Doctoral School of Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary            
            HCEMM-BRC Metabolic Systems Biology Lab, Szeged, Hungary            
            Department of Biochemistry, University of Regina, Regina, SK, Canada            
            Department of Biochemistry, University of Saskatchewan, Saskatoon, SK, Canada            
            Department of Genetics, Eötvös Loránd University, Budapest, Hungary            
            Sequencing Platform, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary            
            Department of Microbiology and Immunology, University of California, San Francisco, United States            
            Faculty of Biology, Technion – Israel Institute of Technology, Haifa, Israel            
            Export Date: 23 January 2020            
            Correspondence Address: Kintses, B.; Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research CentreHungary; email: kintses.balint@brc.hu
Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary            
            HCEMM-BRC Translational Microbiology Lab, Szeged, Hungary            
            Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, Hungary            
            Doctoral School of Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary            
            HCEMM-BRC Metabolic Systems Biology Lab, Szeged, Hungary            
            Department of Biochemistry, University of Regina, Regina, SK, Canada            
            Department of Biochemistry, University of Saskatchewan, Saskatoon, SK, Canada            
            Department of Genetics, Eötvös Loránd University, Budapest, Hungary            
            Sequencing Platform, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary            
            Department of Microbiology and Immunology, University of California, San Francisco, United States            
            Faculty of Biology, Technion – Israel Institute of Technology, Haifa, Israel            
            Cited By :1            
            Export Date: 24 August 2020            
            Correspondence Address: Kintses, B.; Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research CentreHungary; email: kintses.balint@brc.hu
Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary            
            HCEMM-BRC Translational Microbiology Lab, Szeged, Hungary            
            Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, Hungary            
            Doctoral School of Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary            
            HCEMM-BRC Metabolic Systems Biology Lab, Szeged, Hungary            
            Department of Biochemistry, University of Regina, Regina, SK, Canada            
            Department of Biochemistry, University of Saskatchewan, Saskatoon, SK, Canada            
            Department of Genetics, Eötvös Loránd University, Budapest, Hungary            
            Sequencing Platform, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary            
            Department of Microbiology and Immunology, University of California, San Francisco, United States            
            Faculty of Biology, Technion – Israel Institute of Technology, Haifa, Israel            
            Cited By :3            
            Export Date: 8 December 2020            
            Correspondence Address: Kintses, B.; Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research CentreHungary; email: kintses.balint@brc.hu
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Spohn, Réka
AU  - Daruka, Lejla
AU  - Lázár, Viktória
AU  - Martins, Ana
AU  - Vidovics, Fanni
AU  - Grézal, Gábor
AU  - Méhi, Orsolya Katinka
AU  - Kintses, Bálint
AU  - Számel, Mónika
AU  - Jangir, Pramod Kumar
AU  - Csörgő, Bálint
AU  - Györkei, Ádám
AU  - Bódi, Zoltán
AU  - Faragó, Anikó
AU  - Bodai, László
AU  - Földesi, Imre
AU  - Kata, Diána
AU  - Maróti, Gergely
AU  - Pap, Bernadett
AU  - Wirth, Roland
AU  - Papp, Balázs
AU  - Pál, Csaba
TI  - Integrated evolutionary analysis reveals antimicrobial peptides with limited resistance
JF  - NATURE COMMUNICATIONS
J2  - NAT COMMUN
VL  - 10
PY  - 2019
PG  - 13
SN  - 2041-1723
DO  - 10.1038/s41467-019-12364-6
UR  - https://m2.mtmt.hu/api/publication/30865039
ID  - 30865039
N1  - Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary            
            Doctoral School of Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary            
            HCEMM-BRC Metabolic Systems Biology Lab, Szeged, Hungary            
            Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, Hungary            
            Department of Laboratory Medicine, University of Szeged, Szeged, Hungary            
            Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary            
            Department of Biotechnology, University of Szeged, Szeged, Hungary            
            Faculty of Biology, Technion - Israel Institute of Technology, Haifa, Israel            
            University of California, San Francisco, Department of Microbiology and Immunology, San Francisco, CA, United States            
            Cited By :19            
            Export Date: 24 August 2020            
            Correspondence Address: Pál, C.; Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research CentreHungary; email: cpal@brc.hu
Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary            
            Doctoral School of Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary            
            HCEMM-BRC Metabolic Systems Biology Lab, Szeged, Hungary            
            Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, Hungary            
            Department of Laboratory Medicine, University of Szeged, Szeged, Hungary            
            Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary            
            Department of Biotechnology, University of Szeged, Szeged, Hungary            
            Faculty of Biology, Technion - Israel Institute of Technology, Haifa, Israel            
            University of California, San Francisco, Department of Microbiology and Immunology, San Francisco, CA, United States            
            Cited By :32            
            Export Date: 14 January 2021            
            Correspondence Address: Pál, C.; Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research CentreHungary; email: cpal@brc.hu
Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary            
            Doctoral School of Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary            
            HCEMM-BRC Metabolic Systems Biology Lab, Szeged, Hungary            
            Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, Hungary            
            Department of Laboratory Medicine, University of Szeged, Szeged, Hungary            
            Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary            
            Department of Biotechnology, University of Szeged, Szeged, Hungary            
            Faculty of Biology, Technion - Israel Institute of Technology, Haifa, Israel            
            University of California, San Francisco, Department of Microbiology and Immunology, San Francisco, CA, United States            
            Cited By :54            
            Export Date: 30 July 2021            
            Correspondence Address: Pál, C.; Synthetic and Systems Biology Unit, Hungary; email: cpal@brc.hu            
            Chemicals/CAS: chloramphenicol, 134-90-7, 2787-09-9, 56-75-7; indolicidin, 140896-21-5; pexiganan, 172820-23-4, 147664-63-9; polymyxin B, 1404-26-8, 1405-20-5; pr 39, 139637-11-9; protamine, 11061-43-1, 9007-31-2, 9012-00-4; tachyplesin, 118231-04-2; Anti-Infective Agents; Antimicrobial Cationic Peptides
Synthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary            
            Doctoral School of Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary            
            HCEMM-BRC Metabolic Systems Biology Lab, Szeged, Hungary            
            Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, Hungary            
            Department of Laboratory Medicine, University of Szeged, Szeged, Hungary            
            Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary            
            Department of Biotechnology, University of Szeged, Szeged, Hungary            
            Faculty of Biology, Technion - Israel Institute of Technology, Haifa, Israel            
            University of California, San Francisco, Department of Microbiology and Immunology, San Francisco, CA, United States            
            Cited By :58            
            Export Date: 25 August 2021            
            Correspondence Address: Pál, C.; Synthetic and Systems Biology Unit, Hungary; email: cpal@brc.hu
AB  - Antimicrobial peptides (AMPs) are promising antimicrobials, however, the potential of bacterial resistance is a major concern. Here we systematically study the evolution of resistance to 14 chemically diverse AMPs and 12 antibiotics in Escherichia coli. Our work indicates that evolution of resistance against certain AMPs, such as tachyplesin II and cecropin P1, is limited. Resistance level provided by point mutations and gene amplification is very low and antibiotic-resistant bacteria display no cross-resistance to these AMPs. Moreover, genomic fragments derived from a wide range of soil bacteria confer no detectable resistance against these AMPs when introduced into native host bacteria on plasmids. We have found that simple physicochemical features dictate bacterial propensity to evolve resistance against AMPs. Our work could serve as a promising source for the development of new AMP-based therapeutics less prone to resistance, a feature necessary to avoid any possible interference with our innate immune system.
LA  - English
DB  - MTMT
ER  -