TY - THES AU - Alasztics, Bálint TI - NOVEL MOLECULAR BIOLOGICAL MARKERS OF PREECLAMPSIA PY - 2023 DO - 10.14753/SE.2023.2919 UR - https://m2.mtmt.hu/api/publication/34582372 ID - 34582372 LA - English DB - MTMT ER - TY - JOUR AU - Alasztics, Bálint AU - Kovács, Árpád Ferenc AU - Pállinger, Éva AU - Szabó-Taylor, Katalin AU - Szabó, Gábor AU - Molvarec, Attila AU - Koller, Ákos AU - Rigó, János TI - Upregulation of exofacial peroxiredoxin-thioredoxin system of lymphocytes and monocytes in preeclampsia JF - PREGNANCY HYPERTENSION J2 - PREGNANCY HYPERTENS VL - 31 PY - 2023 SP - 54 EP - 59 PG - 6 SN - 2210-7789 DO - 10.1016/j.preghy.2022.12.002 UR - https://m2.mtmt.hu/api/publication/33334444 ID - 33334444 LA - English DB - MTMT ER - TY - JOUR AU - Csomó, Krisztián Benedek AU - Alasztics, Bálint AU - Sándor, A. P. AU - Belik, Andrea AU - Varga, Gábor AU - Hrabák, András AU - Kukor, Zoltán TI - Characterization of oxidation of glutathione by cytochrome c JF - JOURNAL OF BIOENERGETICS AND BIOMEMBRANES J2 - J BIOENERG BIOMEMBR VL - 54 PY - 2022 IS - 1 SP - 1 EP - 8 PG - 8 SN - 0145-479X DO - 10.1007/s10863-021-09926-z UR - https://m2.mtmt.hu/api/publication/32534532 ID - 32534532 LA - English DB - MTMT ER - TY - JOUR AU - Alasztics, Bálint AU - Kovács, Árpád Ferenc AU - Molvarec, Attila AU - Koller, Ákos AU - Szabó, Gábor AU - Fekete, Nóra AU - Buzás, Edit Irén AU - Pállinger, Éva AU - Rigó, János TI - Platelet-derived extracellular vesicles may contribute to the hypercoagulable state in preeclampsia JF - JOURNAL OF REPRODUCTIVE IMMUNOLOGY J2 - J REPROD IMMUNOL VL - 148 PY - 2021 PG - 6 SN - 0165-0378 DO - 10.1016/j.jri.2021.103380 UR - https://m2.mtmt.hu/api/publication/32201054 ID - 32201054 LA - English DB - MTMT ER - TY - JOUR AU - Pánczél, Zita AU - Kukor, Zoltán AU - Supák, Dorina AU - Kovács, Bence Géza AU - Kecskeméti, András AU - Czizel, Rita AU - Djurecz, Magdolna AU - Alasztics, Bálint AU - Csomó, Krisztián Benedek AU - Hrabák, András AU - Valent, Sándor TI - Pravastatin induces NO synthesis by enhancing microsomal arginine uptake in healthy and preeclamptic placentas JF - BMC PREGNANCY AND CHILDBIRTH J2 - BMC PREGNANCY CHILDB VL - 19 PY - 2019 IS - 1 PG - 9 SN - 1471-2393 DO - 10.1186/s12884-019-2507-0 UR - https://m2.mtmt.hu/api/publication/30929732 ID - 30929732 LA - English DB - MTMT ER - TY - JOUR AU - Biró, Orsolya AU - Fóthi, Ábel AU - Alasztics, Bálint AU - Nagy, Bálint AU - Orbán, Tamás I. AU - Rigó, János TI - Circulating exosomal and Argonaute-bound microRNAs in preeclampsia JF - GENE J2 - GENE VL - 692 ET - 0 PY - 2019 SP - 138 EP - 144 PG - 7 SN - 0378-1119 DO - 10.1016/j.gene.2019.01.012 UR - https://m2.mtmt.hu/api/publication/30401681 ID - 30401681 AB - Introduction microRNAs (miRNAs) play important role in the regulation of placental development, and abnormal miRNA expression is associated with preeclampsia (PE). miRNAs are released from trophoblast cells to maternal blood flow, where they are highly stable, being encapsulated inside extracellular vesicles, like exosomes or bound to Argonaute proteins. In PE, placental dysfunction leads to aberrant extracellular miRNA secretion. hsa-miR-210 is a hypoxia-sensitive miRNA found to be upregulated in PE; however, it is unknown whether it is the cause or the consequence of the disease. Objective Our aim was to analyze the expression of several miRNAs, including hsa-miR-210 in placenta, exosome and Ago-bound fractions comparing normal (N) and PE pregnancies. We performed in vitro analyses of extracellular hsa-miR-210 secretion of trophoblast cell cultures (of villous and extravillous origin) under hypoxic condition. Methods PE and N placenta samples were collected from C-sections, and blood samples were drawn from each pregnant woman in the third trimester. HTR-8 and JAR cell lines were cultured in exosome-free media and treated with hypoxia-mimetic agents. Exosome and Ago-bound fractions were isolated by membrane affinity spin column method from plasma and cell media. Short RNAs were extracted from exosomes and vesicle-free fractions, and total-RNA was isolated from the placenta samples. The RNA purity and concentration were measured by spectrophotometry. Expression analysis was carried out by qPCR with specific primers to target and reference miRNAs. Results The level of hsa-miR-210 was significantly higher in PE placentas, which could cause a minor increase of exosomal and a high elevation of Ago-bound miR-210 in circulation. Hypoxia lead to intracellular hsa-miR-210 upregulation in trophoblast cell lines. In extravillous cell (HTR-8) media, only the level of exosomal hsa-miR-210 was increased but no change in Ago-bound hsa-miR-210 level was observed. In contrast, in villous cell (JAR) media, the level of exosomal hsa-miR-210 was increased and enhanced release of Ago-bound hsa-miR-210 was also observed. Conclusion Based on our data, we postulate that in PE, exosomal hsa-miR-210 is secreted actively from the trophoblast, and by intercellular communication, it may have a role in disease etiology. In addition, there is a passive release of Ago-bound hsa-miR-210 into the circulation, which may represent by-products of cell-death and is thereby a possible consequence of the disease. LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Árpád Ferenc AU - Láng, Orsolya AU - Turiák, Lilla AU - Ács, András AU - Kőhidai, László AU - Fekete, Nóra AU - Alasztics, Bálint AU - Mészáros, Tamás AU - Buzás, Edit Irén AU - Rigó, János AU - Pállinger, Éva TI - Author Correction: The impact of circulating preeclampsia-associated extracellular vesicles on the migratory activity and phenotype of THP-1 monocytic cells JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 8 PY - 2018 IS - 1 PG - 1 SN - 2045-2322 DO - 10.1038/s41598-018-29611-3 UR - https://m2.mtmt.hu/api/publication/3408479 ID - 3408479 LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Árpád Ferenc AU - Láng, Orsolya AU - Turiák, Lilla AU - Ács, András AU - Kőhidai, László AU - Fekete, Nóra AU - Alasztics, Bálint AU - Mészáros, Tamás AU - Buzás, Edit Irén AU - Rigó, János AU - Pállinger, Éva TI - The impact of circulating preeclampsia-associated extracellular vesicles on the migratory activity and phenotype of THP-1 monocytic cells JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 8 PY - 2018 IS - 1 PG - 12 SN - 2045-2322 DO - 10.1038/s41598-018-23706-7 UR - https://m2.mtmt.hu/api/publication/3366649 ID - 3366649 N1 - Department of Genetics Cell- and Immunobiology, Semmelweis University, Budapest, Hungary MS Proteomics Research Group, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary 1st Department of Obstetrics and Gynaecology, Semmelweis University, Budapest, Hungary Seroscience Ltd, Budapest, Hungary Nanomedicine Research and Education Center, Institute of Pathophysiology, Semmelweis University, Budapest, Hungary MTA-SE Immunoproteogenomics Extracellular Vesicle Research Group, Budapest, Hungary Cited By :22 Export Date: 3 February 2024 Correspondence Address: Kovács, Á.F.; Department of Genetics Cell- and Immunobiology, Hungary; email: kovacs.arpad@med.semmelweis-univ.hu LA - English DB - MTMT ER - TY - JOUR AU - Biró, Orsolya AU - Alasztics, Bálint AU - Molvarec, Attila AU - Joó, József Gábor AU - Nagy, Bálint AU - Rigó, János TI - Various levels of circulating exosomal total-miRNA and miR-210 hypoxamiR in different forms of pregnancy hypertension JF - PREGNANCY HYPERTENSION J2 - PREGNANCY HYPERTENS VL - 10 PY - 2017 SP - 207 EP - 212 PG - 6 SN - 2210-7789 DO - 10.1016/j.preghy.2017.09.002 UR - https://m2.mtmt.hu/api/publication/3273020 ID - 3273020 AB - Introduction: Hypertension is a common complication during pregnancy, affecting 10% of pregnant women worldwide. Several microRNA (miRNA) were shown to be involved in hypertensive disorders of pregnancy. In preeclampsia (PE), placental dysfunction causes the enhanced release of extracellular vesicle-derived miRNAs. The hypoxia-sensitive hsa-mir-210 is the most common PE-associated miRNA, but its exosomal profile has not been investigated. Objectives: Our aims were to measure exosomal total-miRNA concentration and to perform expression analysis of circulating exosomal hsa-miR-210 in women affected by chronic hypertension (CHT) gestational hypertension (GHT) or PE. Materials and methods: We collected plasma samples from women with CHT, GHT, PE (moderate: mPE and severe: sPE) and from normotensive pregnancies. Exosomal miRNAs were extracted and miRNA concentration was measured. RT-PCR was carried out with hsa-miR-210-3p-specific primers and relative expression was calculated using the comparative Ct method. Results: The total-miRNA concentration was different in the disease subgroups, and was significantly higher in mPE and sPE compared to the other groups. We found a significant difference in the relative exosomal hsa-miR-210-3p expression between all hypertensive groups compared to the normotensive samples, but significant upregulation was only observed in case of mPE and sPE patients. Both the level of total-miRNA and hsa-miR-210 expression was higher in case of severe PE. Conclusions: The level of circulating exosomal total-miRNA and hsa-miR-210 was elevated in women with PE, and it was higher in the severe form. We showed that hsa-miR-210 is secreted via exosomes, which may have a role in the pathomechanism of the disease. © 2017 International Society for the Study of Hypertension in Pregnancy. LA - English DB - MTMT ER - TY - JOUR AU - Biró, Orsolya AU - Fóthi, Ábel AU - Alasztics, Bálint AU - Molvarec, Attila AU - Tamás, Orbán AU - Rigó, János TI - The expression profile of miR-517 family members in preeclamptic placenta and circulating exosome samples JF - PREGNANCY HYPERTENSION J2 - PREGNANCY HYPERTENS VL - 9 PY - 2017 SP - 46 PG - 1 SN - 2210-7789 DO - 10.1016/j.preghy.2017.07.098 UR - https://m2.mtmt.hu/api/publication/3269946 ID - 3269946 AB - Introduction Preeclampsia is the major cause of maternal and fetal morbidity and mortality, affecting 3–8% of pregnancies worldwide. Recent findings suggest that placenta-specific miRNAs may contribute to pregnancy-related diseases. The miR- 517 family was proposed as a major contributing factor to trophoblast dysfunction. Objectives Our aim was to analyze the expression of the miR-517 family members in placenta and circulating exosome samples comparing normal (N) and preeclamptic (PE) pregnancies. Methods PE and N placenta samples were collected from C-sections and stored in RNA-later. Blood samples were drawn from each pregnant in the third trimester. Plasma was separated, and exosomes were isolated by precipitation method. Short RNAs were extracted from the diluted exosomes on column, and total-RNA was isolated from the placenta samples with TRIzol. Expression analysis was carried out by qPCR with primers specific to miR-517a/b, miR-517c, and mir-517-5p; and relative expression was calculated using the comparative Ct method. Results We found significant differences in the expression of all assessed miRNAs between PE and N pregnancies. The level of placental hsa-miR-517a/b, hsa-miR-517c, and hsa-miR-517-5p was ∼2–3 times higher, comparing affected and control groups. The deviation was more pronounced in exosomal samples since all three miRNAs were expressed 4–6-times higher in the disease group. As expected, the level of miR-517a/b and c was ∼100 times higher than the passenger strand, miR-517-5p in both types of samples. Based on these data, it can be assumed that the sorting of these miRNAs varies between affected and normal pregnancy. Conclusion Placenta-specific miRNAs are believed to play important role in the regulation of pregnancy. We found that miR-517 family members are overexpressed both in preeclamptic placenta and circulating exosome samples. It can be assumed that the sorting of these miRNAs varies between affected and normal pregnancy. LA - English DB - MTMT ER -