@mastersthesis{MTMT:34582372, title = {NOVEL MOLECULAR BIOLOGICAL MARKERS OF PREECLAMPSIA}, url = {https://m2.mtmt.hu/api/publication/34582372}, author = {Alasztics, Bálint}, doi = {10.14753/SE.2023.2919}, unique-id = {34582372}, year = {2023}, orcid-numbers = {Alasztics, Bálint/0000-0002-4011-8439} } @article{MTMT:33334444, title = {Upregulation of exofacial peroxiredoxin-thioredoxin system of lymphocytes and monocytes in preeclampsia}, url = {https://m2.mtmt.hu/api/publication/33334444}, author = {Alasztics, Bálint and Kovács, Árpád Ferenc and Pállinger, Éva and Szabó-Taylor, Katalin and Szabó, Gábor and Molvarec, Attila and Koller, Ákos and Rigó, János}, doi = {10.1016/j.preghy.2022.12.002}, journal-iso = {PREGNANCY HYPERTENS}, journal = {PREGNANCY HYPERTENSION}, volume = {31}, unique-id = {33334444}, issn = {2210-7789}, year = {2023}, eissn = {2210-7797}, pages = {54-59}, orcid-numbers = {Alasztics, Bálint/0000-0002-4011-8439; Kovács, Árpád Ferenc/0000-0002-7742-160X; Pállinger, Éva/0000-0002-5789-0951; Szabó-Taylor, Katalin/0000-0002-4763-3521; Szabó, Gábor/0000-0002-8573-8260; Molvarec, Attila/0000-0002-3229-3034; Koller, Ákos/0000-0003-3256-8701; Rigó, János/0000-0003-2762-6516} } @article{MTMT:32534532, title = {Characterization of oxidation of glutathione by cytochrome c}, url = {https://m2.mtmt.hu/api/publication/32534532}, author = {Csomó, Krisztián Benedek and Alasztics, Bálint and Sándor, A. P. and Belik, Andrea and Varga, Gábor and Hrabák, András and Kukor, Zoltán}, doi = {10.1007/s10863-021-09926-z}, journal-iso = {J BIOENERG BIOMEMBR}, journal = {JOURNAL OF BIOENERGETICS AND BIOMEMBRANES}, volume = {54}, unique-id = {32534532}, issn = {0145-479X}, year = {2022}, eissn = {1573-6881}, pages = {1-8}, orcid-numbers = {Csomó, Krisztián Benedek/0000-0002-3749-6391; Alasztics, Bálint/0000-0002-4011-8439; Belik, Andrea/0000-0002-8146-2009; Varga, Gábor/0000-0002-5506-8198; Hrabák, András/0000-0002-7818-6509; Kukor, Zoltán/0000-0002-7250-2160} } @article{MTMT:32201054, title = {Platelet-derived extracellular vesicles may contribute to the hypercoagulable state in preeclampsia}, url = {https://m2.mtmt.hu/api/publication/32201054}, author = {Alasztics, Bálint and Kovács, Árpád Ferenc and Molvarec, Attila and Koller, Ákos and Szabó, Gábor and Fekete, Nóra and Buzás, Edit Irén and Pállinger, Éva and Rigó, János}, doi = {10.1016/j.jri.2021.103380}, journal-iso = {J REPROD IMMUNOL}, journal = {JOURNAL OF REPRODUCTIVE IMMUNOLOGY}, volume = {148}, unique-id = {32201054}, issn = {0165-0378}, year = {2021}, eissn = {1872-7603}, orcid-numbers = {Alasztics, Bálint/0000-0002-4011-8439; Kovács, Árpád Ferenc/0000-0002-7742-160X; Molvarec, Attila/0000-0002-3229-3034; Koller, Ákos/0000-0003-3256-8701; Szabó, Gábor/0000-0002-8573-8260; Buzás, Edit Irén/0000-0002-3744-206X; Pállinger, Éva/0000-0002-5789-0951; Rigó, János/0000-0003-2762-6516} } @article{MTMT:30929732, title = {Pravastatin induces NO synthesis by enhancing microsomal arginine uptake in healthy and preeclamptic placentas}, url = {https://m2.mtmt.hu/api/publication/30929732}, author = {Pánczél, Zita and Kukor, Zoltán and Supák, Dorina and Kovács, Bence Géza and Kecskeméti, András and Czizel, Rita and Djurecz, Magdolna and Alasztics, Bálint and Csomó, Krisztián Benedek and Hrabák, András and Valent, Sándor}, doi = {10.1186/s12884-019-2507-0}, journal-iso = {BMC PREGNANCY CHILDB}, journal = {BMC PREGNANCY AND CHILDBIRTH}, volume = {19}, unique-id = {30929732}, issn = {1471-2393}, year = {2019}, eissn = {1471-2393}, orcid-numbers = {Pánczél, Zita/0000-0003-4496-6280; Kukor, Zoltán/0000-0002-7250-2160; Kovács, Bence Géza/0000-0002-5578-1858; Kecskeméti, András/0000-0001-5314-3927; Alasztics, Bálint/0000-0002-4011-8439; Csomó, Krisztián Benedek/0000-0002-3749-6391; Hrabák, András/0000-0002-7818-6509; Valent, Sándor/0000-0003-1098-1861} } @article{MTMT:30401681, title = {Circulating exosomal and Argonaute-bound microRNAs in preeclampsia}, url = {https://m2.mtmt.hu/api/publication/30401681}, author = {Biró, Orsolya and Fóthi, Ábel and Alasztics, Bálint and Nagy, Bálint and Orbán, Tamás I. and Rigó, János}, doi = {10.1016/j.gene.2019.01.012}, journal-iso = {GENE}, journal = {GENE}, volume = {692}, unique-id = {30401681}, issn = {0378-1119}, abstract = {Introduction microRNAs (miRNAs) play important role in the regulation of placental development, and abnormal miRNA expression is associated with preeclampsia (PE). miRNAs are released from trophoblast cells to maternal blood flow, where they are highly stable, being encapsulated inside extracellular vesicles, like exosomes or bound to Argonaute proteins. In PE, placental dysfunction leads to aberrant extracellular miRNA secretion. hsa-miR-210 is a hypoxia-sensitive miRNA found to be upregulated in PE; however, it is unknown whether it is the cause or the consequence of the disease. Objective Our aim was to analyze the expression of several miRNAs, including hsa-miR-210 in placenta, exosome and Ago-bound fractions comparing normal (N) and PE pregnancies. We performed in vitro analyses of extracellular hsa-miR-210 secretion of trophoblast cell cultures (of villous and extravillous origin) under hypoxic condition. Methods PE and N placenta samples were collected from C-sections, and blood samples were drawn from each pregnant woman in the third trimester. HTR-8 and JAR cell lines were cultured in exosome-free media and treated with hypoxia-mimetic agents. Exosome and Ago-bound fractions were isolated by membrane affinity spin column method from plasma and cell media. Short RNAs were extracted from exosomes and vesicle-free fractions, and total-RNA was isolated from the placenta samples. The RNA purity and concentration were measured by spectrophotometry. Expression analysis was carried out by qPCR with specific primers to target and reference miRNAs. Results The level of hsa-miR-210 was significantly higher in PE placentas, which could cause a minor increase of exosomal and a high elevation of Ago-bound miR-210 in circulation. Hypoxia lead to intracellular hsa-miR-210 upregulation in trophoblast cell lines. In extravillous cell (HTR-8) media, only the level of exosomal hsa-miR-210 was increased but no change in Ago-bound hsa-miR-210 level was observed. In contrast, in villous cell (JAR) media, the level of exosomal hsa-miR-210 was increased and enhanced release of Ago-bound hsa-miR-210 was also observed. Conclusion Based on our data, we postulate that in PE, exosomal hsa-miR-210 is secreted actively from the trophoblast, and by intercellular communication, it may have a role in disease etiology. In addition, there is a passive release of Ago-bound hsa-miR-210 into the circulation, which may represent by-products of cell-death and is thereby a possible consequence of the disease.}, year = {2019}, eissn = {1879-0038}, pages = {138-144}, orcid-numbers = {Biró, Orsolya/0000-0002-4300-3602; Alasztics, Bálint/0000-0002-4011-8439; Nagy, Bálint/0000-0002-0295-185X; Orbán, Tamás I./0000-0002-3424-3428; Rigó, János/0000-0003-2762-6516} } @article{MTMT:3408479, title = {Author Correction: The impact of circulating preeclampsia-associated extracellular vesicles on the migratory activity and phenotype of THP-1 monocytic cells}, url = {https://m2.mtmt.hu/api/publication/3408479}, author = {Kovács, Árpád Ferenc and Láng, Orsolya and Turiák, Lilla and Ács, András and Kőhidai, László and Fekete, Nóra and Alasztics, Bálint and Mészáros, Tamás and Buzás, Edit Irén and Rigó, János and Pállinger, Éva}, doi = {10.1038/s41598-018-29611-3}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {8}, unique-id = {3408479}, issn = {2045-2322}, year = {2018}, eissn = {2045-2322}, orcid-numbers = {Kovács, Árpád Ferenc/0000-0002-7742-160X; Láng, Orsolya/0000-0002-2787-2154; Kőhidai, László/0000-0002-9002-0296; Alasztics, Bálint/0000-0002-4011-8439; Buzás, Edit Irén/0000-0002-3744-206X; Rigó, János/0000-0003-2762-6516; Pállinger, Éva/0000-0002-5789-0951} } @article{MTMT:3366649, title = {The impact of circulating preeclampsia-associated extracellular vesicles on the migratory activity and phenotype of THP-1 monocytic cells}, url = {https://m2.mtmt.hu/api/publication/3366649}, author = {Kovács, Árpád Ferenc and Láng, Orsolya and Turiák, Lilla and Ács, András and Kőhidai, László and Fekete, Nóra and Alasztics, Bálint and Mészáros, Tamás and Buzás, Edit Irén and Rigó, János and Pállinger, Éva}, doi = {10.1038/s41598-018-23706-7}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {8}, unique-id = {3366649}, issn = {2045-2322}, year = {2018}, eissn = {2045-2322}, orcid-numbers = {Kovács, Árpád Ferenc/0000-0002-7742-160X; Láng, Orsolya/0000-0002-2787-2154; Kőhidai, László/0000-0002-9002-0296; Alasztics, Bálint/0000-0002-4011-8439; Buzás, Edit Irén/0000-0002-3744-206X; Rigó, János/0000-0003-2762-6516; Pállinger, Éva/0000-0002-5789-0951} } @article{MTMT:3273020, title = {Various levels of circulating exosomal total-miRNA and miR-210 hypoxamiR in different forms of pregnancy hypertension}, url = {https://m2.mtmt.hu/api/publication/3273020}, author = {Biró, Orsolya and Alasztics, Bálint and Molvarec, Attila and Joó, József Gábor and Nagy, Bálint and Rigó, János}, doi = {10.1016/j.preghy.2017.09.002}, journal-iso = {PREGNANCY HYPERTENS}, journal = {PREGNANCY HYPERTENSION}, volume = {10}, unique-id = {3273020}, issn = {2210-7789}, abstract = {Introduction: Hypertension is a common complication during pregnancy, affecting 10% of pregnant women worldwide. Several microRNA (miRNA) were shown to be involved in hypertensive disorders of pregnancy. In preeclampsia (PE), placental dysfunction causes the enhanced release of extracellular vesicle-derived miRNAs. The hypoxia-sensitive hsa-mir-210 is the most common PE-associated miRNA, but its exosomal profile has not been investigated. Objectives: Our aims were to measure exosomal total-miRNA concentration and to perform expression analysis of circulating exosomal hsa-miR-210 in women affected by chronic hypertension (CHT) gestational hypertension (GHT) or PE. Materials and methods: We collected plasma samples from women with CHT, GHT, PE (moderate: mPE and severe: sPE) and from normotensive pregnancies. Exosomal miRNAs were extracted and miRNA concentration was measured. RT-PCR was carried out with hsa-miR-210-3p-specific primers and relative expression was calculated using the comparative Ct method. Results: The total-miRNA concentration was different in the disease subgroups, and was significantly higher in mPE and sPE compared to the other groups. We found a significant difference in the relative exosomal hsa-miR-210-3p expression between all hypertensive groups compared to the normotensive samples, but significant upregulation was only observed in case of mPE and sPE patients. Both the level of total-miRNA and hsa-miR-210 expression was higher in case of severe PE. Conclusions: The level of circulating exosomal total-miRNA and hsa-miR-210 was elevated in women with PE, and it was higher in the severe form. We showed that hsa-miR-210 is secreted via exosomes, which may have a role in the pathomechanism of the disease. © 2017 International Society for the Study of Hypertension in Pregnancy.}, keywords = {PREECLAMPSIA; microRNA; exosome; Maternal circulation}, year = {2017}, eissn = {2210-7797}, pages = {207-212}, orcid-numbers = {Biró, Orsolya/0000-0002-4300-3602; Alasztics, Bálint/0000-0002-4011-8439; Molvarec, Attila/0000-0002-3229-3034; Joó, József Gábor/0000-0001-9820-6514; Nagy, Bálint/0000-0002-0295-185X; Rigó, János/0000-0003-2762-6516} } @article{MTMT:3269946, title = {The expression profile of miR-517 family members in preeclamptic placenta and circulating exosome samples}, url = {https://m2.mtmt.hu/api/publication/3269946}, author = {Biró, Orsolya and Fóthi, Ábel and Alasztics, Bálint and Molvarec, Attila and Tamás, Orbán and Rigó, János}, doi = {10.1016/j.preghy.2017.07.098}, journal-iso = {PREGNANCY HYPERTENS}, journal = {PREGNANCY HYPERTENSION}, volume = {9}, unique-id = {3269946}, issn = {2210-7789}, abstract = {Introduction Preeclampsia is the major cause of maternal and fetal morbidity and mortality, affecting 3–8% of pregnancies worldwide. Recent findings suggest that placenta-specific miRNAs may contribute to pregnancy-related diseases. The miR- 517 family was proposed as a major contributing factor to trophoblast dysfunction. Objectives Our aim was to analyze the expression of the miR-517 family members in placenta and circulating exosome samples comparing normal (N) and preeclamptic (PE) pregnancies. Methods PE and N placenta samples were collected from C-sections and stored in RNA-later. Blood samples were drawn from each pregnant in the third trimester. Plasma was separated, and exosomes were isolated by precipitation method. Short RNAs were extracted from the diluted exosomes on column, and total-RNA was isolated from the placenta samples with TRIzol. Expression analysis was carried out by qPCR with primers specific to miR-517a/b, miR-517c, and mir-517-5p; and relative expression was calculated using the comparative Ct method. Results We found significant differences in the expression of all assessed miRNAs between PE and N pregnancies. The level of placental hsa-miR-517a/b, hsa-miR-517c, and hsa-miR-517-5p was ∼2–3 times higher, comparing affected and control groups. The deviation was more pronounced in exosomal samples since all three miRNAs were expressed 4–6-times higher in the disease group. As expected, the level of miR-517a/b and c was ∼100 times higher than the passenger strand, miR-517-5p in both types of samples. Based on these data, it can be assumed that the sorting of these miRNAs varies between affected and normal pregnancy. Conclusion Placenta-specific miRNAs are believed to play important role in the regulation of pregnancy. We found that miR-517 family members are overexpressed both in preeclamptic placenta and circulating exosome samples. It can be assumed that the sorting of these miRNAs varies between affected and normal pregnancy.}, year = {2017}, eissn = {2210-7797}, pages = {46-47}, orcid-numbers = {Biró, Orsolya/0000-0002-4300-3602; Alasztics, Bálint/0000-0002-4011-8439; Molvarec, Attila/0000-0002-3229-3034; Rigó, János/0000-0003-2762-6516} }