@CONFERENCE{MTMT:33693549,
	title = {NICOTINIC ACID CATABOLISM IN ASPERGILLUS NIDULANS: AN EXAMPLE OF CONVERGENT EVOLUTION},
	url = {https://m2.mtmt.hu/api/publication/33693549},
	author = {Kozma-Bognárné Hamari, Zsuzsanna and Eszter, Bokor and Ámon, Judit and Varga, Mónika and Szekeres, András and Hegedüs, Zsófia and Tamás, Jakusch and Szakonyi, Zsolt and Flipphi, Michel Johannes Anthonie and Vágvölgyi, Csaba and Gácser, Attila and Claudio, Scazzocchio},
	booktitle = {16th European Conference on Fungal Genetics: Programme & Abstracts},
	unique-id = {33693549},
	abstract = {Nicotinic acid (niacin) is a precursor of NAD and NADP and thus an essential metabolite. Its degradation has been previously described only in bacteria.
		We describe, for the first time, a complete eukaryotic pathway of nico- tinic acid (NA) catabolism. The genes are organised in three co-regu- lated gene clusters, which encode eight enzymes, two transporters and the pathway specific transcription factor. The pathway is conserved; and its variable organisation in the Pezizomycotina illustrates cluster evolution and horizontal gene transfer.
		Reverse genetics was coupled with state-of-the-art chemical charac- terisation of each intermediate. The first step, the conversion of NA to 6-hydroxynicotinic acid (6-NA) is common to prokaryotic and eukary- otic pathways, even if catalysed by independently evolved enzymes. While two downstream metabolites, 2,5-dihydroxypyridine and 2,3,6-tri- hydroxypyridine, are common to various prokaryotic routes, other steps and metabolites are unprecedented: 3-hydroxypiperidine-2,6-dione and 5,6-dihydroxypiperidine-2-one intermediate metabolites have not been identified previously in any organism, the latter being a completely nov- el chemical compound. Furthermore, the hydrolytic N-C ring opening results in α-hydroxyglutaramate, a compound not detected in analo- gous prokaryotic pathways. Remarkably, the physiological inducer of the whole pathway is a near-terminal intermediate metabolite: 5,6-di- hydroxypiperidine-2-one.
		While most steps are cytosolic, two steps take place in the peroxi- somes. 6-NA monooxygenase (HxnX) enters peroxisomes through a canonical PTS-1 signal, while HxnW, a polyol dehydrogenase, is co-transported by piggybacking HxnX.
		The genomic organisation and phylogeny of the pathway cognate genes and proteins, showed that this catabolic pathway is of fungal (Ascomycota) origin and thus it exemplifies convergent evolution of catabolic pathways between fungi and bacteria, where at least four different pathways occur.},
	year = {2023},
	pages = {767-768},
	orcid-numbers = {Kozma-Bognárné Hamari, Zsuzsanna/0000-0001-6374-5083; Ámon, Judit/0000-0002-3234-6167; Szekeres, András/0000-0003-1651-4623; Szakonyi, Zsolt/0000-0003-2432-8409; Vágvölgyi, Csaba/0000-0003-0009-7773}
}

@article{MTMT:33188394,
	title = {The role of the Aspergillus nidulans high mobility group B protein HmbA, the orthologue of Saccharomyces cerevisiae Nhp6p},
	url = {https://m2.mtmt.hu/api/publication/33188394},
	author = {Ámon, Judit and Varga, Gabriella Imola and Pfeiffer, Ilona and Farkas, Zoltán and Karácsony, Zoltán and Hegedüs, Zsófia and Vágvölgyi, Csaba and Kozma-Bognárné Hamari, Zsuzsanna},
	doi = {10.1038/s41598-022-22202-3},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {12},
	unique-id = {33188394},
	issn = {2045-2322},
	abstract = {The mammalian HMGB1 is a high-mobility-group B protein, which is both an architectural and functional element of chromatin. Nhp6p, the extensively studied fungal homologue of HMGB1 in Saccharomyces cerevisiae has pleiotropic physiological functions. Despite the existence of Nhp6p orthologues in filamentous ascomycetes, little is known about their physiological roles besides their contribution to sexual development. Here we study the function of HmbA, the Aspergillus nidulans orthologue of Nhp6p. We show that HmbA influences the utilization of various carbon- and nitrogen sources, stress tolerance, secondary metabolism, hyphae elongation and maintenance of polarized growth. Additionally, by conducting heterologous expression studies, we demonstrate that HmbA and Nhp6p are partially interchangeable. HmbA restores SNR6 transcription and fitness of nhp6AΔBΔ mutant and reverses its heat sensitivity. Nhp6Ap complements several phenotypes of hmbAΔ , including ascospore formation, utilization of various carbon- and nitrogen-sources, radial growth rate, hypha elongation by polarized growth. However, Nhp6Ap does not complement sterigmatocystin production in a hmbAΔ strain. Finally, we also show that HmbA is necessary for the normal expression of the endochitinase chiA , a cell wall re-modeller that is pivotal for the normal mode of maintenance of polar growth.},
	year = {2022},
	eissn = {2045-2322},
	orcid-numbers = {Ámon, Judit/0000-0002-3234-6167; Pfeiffer, Ilona/0000-0003-0680-7596; Vágvölgyi, Csaba/0000-0003-0009-7773; Kozma-Bognárné Hamari, Zsuzsanna/0000-0001-6374-5083}
}

@article{MTMT:33025855,
	title = {A complete nicotinate degradation pathway in the microbial eukaryote Aspergillus nidulans},
	url = {https://m2.mtmt.hu/api/publication/33025855},
	author = {Bokor, Eszter and Ámon, Judit and Varga, Mónika and Szekeres, András and Hegedüs, Zsófia and Jakusch, Tamás and Szakonyi, Zsolt and Flipphi, Michel Johannes Anthonie and Vágvölgyi, Csaba and Gácser, Attila and Scazzocchio, Claudio and Kozma-Bognárné Hamari, Zsuzsanna},
	doi = {10.1038/s42003-022-03684-3},
	journal-iso = {COMMUN BIOL},
	journal = {COMMUNICATIONS BIOLOGY},
	volume = {5},
	unique-id = {33025855},
	abstract = {Several strikingly different aerobic and anaerobic pathways of nicotinate breakdown are extant in bacteria. Here, through reverse genetics and analytical techniques we elucidated in Aspergillus nidulans, a complete eukaryotic nicotinate utilization pathway. The pathway extant in this fungus and other ascomycetes, is quite different from bacterial ones. All intermediate metabolites were identified. The cognate proteins, encoded by eleven genes (hxn) mapping in three clusters are co-regulated by a specific transcription factor. Several enzymatic steps have no prokaryotic equivalent and two metabolites, 3-hydroxypiperidine-2,6-dione and 5,6-dihydroxypiperidine-2-one, have not been identified previously in any organism, the latter being a novel chemical compound. Hydrolytic ring opening results in α-hydroxyglutaramate, a compound not detected in analogous prokaryotic pathways. Our earlier phylogenetic analysis of Hxn proteins together with this complete biochemical pathway illustrates convergent evolution of catabolic pathways between fungi and bacteria.},
	year = {2022},
	eissn = {2399-3642},
	orcid-numbers = {Bokor, Eszter/0000-0003-2338-859X; Ámon, Judit/0000-0002-3234-6167; Szekeres, András/0000-0003-1651-4623; Jakusch, Tamás/0000-0003-0532-5202; Szakonyi, Zsolt/0000-0003-2432-8409; Vágvölgyi, Csaba/0000-0003-0009-7773; Scazzocchio, Claudio/0000-0001-8800-2534; Kozma-Bognárné Hamari, Zsuzsanna/0000-0001-6374-5083}
}

@article{MTMT:32469677,
	title = {Nicotinate degradation in a microbial eukaryote: a novel, complete pathway extant in Aspergillus nidulans},
	url = {https://m2.mtmt.hu/api/publication/32469677},
	author = {Kozma-Bognárné Hamari, Zsuzsanna and Bokor, Eszter and Ámon, Judit and Hegedüs, Zsófia and Varga, Mónika and Szekeres, András and Jakusch, Tamás and Vágvölgyi, Csaba},
	journal-iso = {ACTA MICROBIOL IMMUNOL HUNG},
	journal = {ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA},
	volume = {68},
	unique-id = {32469677},
	issn = {1217-8950},
	year = {2021},
	eissn = {1588-2640},
	pages = {69-70},
	orcid-numbers = {Kozma-Bognárné Hamari, Zsuzsanna/0000-0001-6374-5083; Bokor, Eszter/0000-0003-2338-859X; Ámon, Judit/0000-0002-3234-6167; Szekeres, András/0000-0003-1651-4623; Jakusch, Tamás/0000-0003-0532-5202; Vágvölgyi, Csaba/0000-0003-0009-7773}
}

@article{MTMT:32469634,
	title = {In vitro study of the neofunctionalization of the nicotinate hydroxylase HxnS, a paralogue of the xanthine dehydrogenase HxA},
	url = {https://m2.mtmt.hu/api/publication/32469634},
	author = {Ámon, Judit and Bokor, Eszter and Vágvölgyi, Csaba and Kozma-Bognárné Hamari, Zsuzsanna},
	journal-iso = {ACTA MICROBIOL IMMUNOL HUNG},
	journal = {ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA},
	volume = {68},
	unique-id = {32469634},
	issn = {1217-8950},
	year = {2021},
	eissn = {1588-2640},
	pages = {55-56},
	orcid-numbers = {Ámon, Judit/0000-0002-3234-6167; Bokor, Eszter/0000-0003-2338-859X; Vágvölgyi, Csaba/0000-0003-0009-7773; Kozma-Bognárné Hamari, Zsuzsanna/0000-0001-6374-5083}
}

@article{MTMT:32469613,
	title = {Aberrant chitin deposition of HmbA HMGB protein deficient Aspergillus nidulans},
	url = {https://m2.mtmt.hu/api/publication/32469613},
	author = {Ámon, Judit and Bokor, Eszter and Varga, Gabriella Imola and Vágvölgyi, Csaba and Kozma-Bognárné Hamari, Zsuzsanna},
	journal-iso = {ACTA MICROBIOL IMMUNOL HUNG},
	journal = {ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA},
	volume = {68},
	unique-id = {32469613},
	issn = {1217-8950},
	year = {2021},
	eissn = {1588-2640},
	pages = {5-5},
	orcid-numbers = {Ámon, Judit/0000-0002-3234-6167; Bokor, Eszter/0000-0003-2338-859X; Vágvölgyi, Csaba/0000-0003-0009-7773; Kozma-Bognárné Hamari, Zsuzsanna/0000-0001-6374-5083}
}

@article{MTMT:32258405,
	title = {Genome organization and evolution of a eukaryotic nicotinate co-inducible pathway},
	url = {https://m2.mtmt.hu/api/publication/32258405},
	author = {Bokor, Eszter and Flipphi, Michel Johannes Anthonie and Kocsubé, Sándor and Ámon, Judit and Vágvölgyi, Csaba and Scazzocchio, Claudio and Kozma-Bognárné Hamari, Zsuzsanna},
	doi = {10.1098/rsob.210099},
	journal-iso = {OPEN BIOL},
	journal = {OPEN BIOLOGY},
	volume = {11},
	unique-id = {32258405},
	abstract = {In Aspergillus nidulans a regulon including 11 hxn genes (hxnS, T, R, P, Y, Z, X, W, V, M and N) is inducible by a nicotinate metabolic derivative, repressible by ammonium and under stringent control of the nitrogen-state-sensitive GATA factor AreA and the specific transcription factor HxnR. This is the first report in a eukaryote of the genomic organization of a possibly complete pathway of nicotinate utilization. In A. nidulans the regulon is organized in three distinct clusters, this organization is variable in the Ascomycota. In some Pezizomycotina species all 11 genes map in a single cluster; in others they map in two clusters. This variable organization sheds light on cluster evolution. Instances of gene duplication followed by or simultaneous with integration in the cluster, partial or total cluster loss, and horizontal gene transfer of several genes (including an example of whole cluster re-acquisition in Aspergillus of section Flavi) were detected, together with the incorporation in some clusters of genes not found in the A. nidulans co-regulated regulon, which underlie both the plasticity and the reticulate character of metabolic cluster evolution. This study provides a comprehensive phylogeny of six members of the cluster across representatives of all Ascomycota classes.},
	year = {2021},
	eissn = {2046-2441},
	orcid-numbers = {Bokor, Eszter/0000-0003-2338-859X; Kocsubé, Sándor/0000-0001-7839-0510; Ámon, Judit/0000-0002-3234-6167; Vágvölgyi, Csaba/0000-0003-0009-7773; Scazzocchio, Claudio/0000-0001-8800-2534; Kozma-Bognárné Hamari, Zsuzsanna/0000-0001-6374-5083}
}

@CONFERENCE{MTMT:31354492,
	title = {The nicotinic acid pathway of Aspergillus nidulans includes a reversible conversion to 6-hydroxynicotinic acid},
	url = {https://m2.mtmt.hu/api/publication/31354492},
	author = {Bokor, Eszter and Ámon, Judit and Varga, Mónika and Szekeres, András and Claudio, Scazzocchio and Vágvölgyi, Csaba and Kozma-Bognárné Hamari, Zsuzsanna},
	booktitle = {15th European Conference on Fungal Genetics (ECFG15): Program and Abstracts},
	unique-id = {31354492},
	year = {2020},
	pages = {235-235},
	orcid-numbers = {Bokor, Eszter/0000-0003-2338-859X; Ámon, Judit/0000-0002-3234-6167; Szekeres, András/0000-0003-1651-4623; Vágvölgyi, Csaba/0000-0003-0009-7773; Kozma-Bognárné Hamari, Zsuzsanna/0000-0001-6374-5083}
}

@CONFERENCE{MTMT:31354491,
	title = {Structural homology function predictions for fungal nicotinate catabolising enzymes},
	url = {https://m2.mtmt.hu/api/publication/31354491},
	author = {Bokor, Eszter and Ámon, Judit and Claudio, Scazzocchio and Vágvölgyi, Csaba and Kozma-Bognárné Hamari, Zsuzsanna},
	booktitle = {15th European Conference on Fungal Genetics (ECFG15): Program and Abstracts},
	unique-id = {31354491},
	year = {2020},
	pages = {236-236},
	orcid-numbers = {Bokor, Eszter/0000-0003-2338-859X; Ámon, Judit/0000-0002-3234-6167; Vágvölgyi, Csaba/0000-0003-0009-7773; Kozma-Bognárné Hamari, Zsuzsanna/0000-0001-6374-5083}
}

@CONFERENCE{MTMT:31354464,
	title = {In vitro enzyme evolution of Purine Hydroxylase I (HxA) and Purine Hydroxylase II (HxnS)},
	url = {https://m2.mtmt.hu/api/publication/31354464},
	author = {Ámon, Judit and Bokor, Eszter and Vágvölgyi, Csaba and Claudio, Scazzocchio and Kozma-Bognárné Hamari, Zsuzsanna},
	booktitle = {15th European Conference on Fungal Genetics (ECFG15): Program and Abstracts},
	unique-id = {31354464},
	year = {2020},
	pages = {235-236},
	orcid-numbers = {Ámon, Judit/0000-0002-3234-6167; Bokor, Eszter/0000-0003-2338-859X; Vágvölgyi, Csaba/0000-0003-0009-7773; Kozma-Bognárné Hamari, Zsuzsanna/0000-0001-6374-5083}
}