TY - JOUR AU - Kúthy-Sutus, Enikő AU - Kharrat, Bayan AU - Gábor, Erika AU - Csordás, Gábor AU - Sinka, Rita AU - Honti, Viktor TI - A Novel Method for Primary Blood Cell Culturing and Selection in Drosophila melanogaster JF - CELLS J2 - CELLS-BASEL VL - 12 PY - 2023 IS - 1 PG - 15 SN - 2073-4409 DO - 10.3390/cells12010024 UR - https://m2.mtmt.hu/api/publication/33555087 ID - 33555087 N1 - Export Date: 24 January 2023 AB - The blood cells of the fruit fly Drosophila melanogaster show many similarities to their vertebrate counterparts, both in their functions and their differentiation. In the past decades, a wide palette of immunological and transgenic tools and methods have been developed to study hematopoiesis in the Drosophila larva. However, the in vivo observation of blood cells is technically restricted by the limited transparency of the body and the difficulty in keeping the organism alive during imaging. Here we describe an improved ex vivo culturing method that allows effective visualization and selection of live blood cells in primary cultures derived from Drosophila larvae. Our results show that cultured hemocytes accurately represent morphological and functional changes following immune challenges and in case of genetic alterations. Since cell culturing has hugely contributed to the understanding of the physiological properties of vertebrate blood cells, this method provides a versatile tool for studying Drosophila hemocyte differentiation and functions ex vivo. LA - English DB - MTMT ER - TY - CONF AU - Kúthy-Sutus, Enikő AU - Gábor, Erika AU - Kharrat, Bayan AU - Jankovics, Ferenc AU - Sinka, Rita AU - Honti, Viktor TI - Studying Drosophila blood cell plasticity using primary blood cell cultures T2 - Straub-Napok 2022 PY - 2022 SP - 1 UR - https://m2.mtmt.hu/api/publication/34689202 ID - 34689202 LA - English DB - MTMT ER - TY - JOUR AU - Csordás, Gábor AU - Gábor, Erika AU - Honti, Viktor TI - There and back again: The mechanisms of differentiation and transdifferentiation in Drosophila blood cells JF - DEVELOPMENTAL BIOLOGY J2 - DEV BIOL VL - 469 PY - 2021 SP - 135 EP - 143 PG - 9 SN - 0012-1606 DO - 10.1016/j.ydbio.2020.10.006 UR - https://m2.mtmt.hu/api/publication/31743832 ID - 31743832 N1 - Cited By :5 Export Date: 14 June 2022 CODEN: DEBIA LA - English DB - MTMT ER - TY - JOUR AU - Kharrat, B AU - Gábor, Erika AU - Sinka, Rita AU - Honti, Viktor TI - Function and Interacting Partners of Headcase in Drosophila Melanogaster JF - IMMUNOLÓGIAI SZEMLE J2 - IMMUNOLÓGIAI SZEMLE VL - 12 PY - 2020 IS - 3 SP - 25 EP - 25 PG - 1 SN - 2061-0203 UR - https://m2.mtmt.hu/api/publication/31891672 ID - 31891672 LA - English DB - MTMT ER - TY - JOUR AU - Gábor, Erika AU - Varga, GI AU - Géczi, Aliz AU - Bayan, K AU - Migh, E AU - Beleon, A AU - Horvath, P AU - Szeplaki, B AU - Enyedi, M AU - Pinter, L AU - Haracska, L AU - Honti, Viktor TI - Vérsejtek transzdifferenciálódásának vizsgálata a mélytanulás módszerével Drosophila melanogasterben JF - IMMUNOLÓGIAI SZEMLE J2 - IMMUNOLÓGIAI SZEMLE VL - 12 PY - 2020 IS - 3 SP - 8 EP - 9 PG - 2 SN - 2061-0203 UR - https://m2.mtmt.hu/api/publication/31891648 ID - 31891648 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Gábor, Erika AU - Cinege, Gyöngyi Ilona AU - Csordás, Gábor AU - Rusvai, Miklós AU - Honti, Viktor AU - Kolics, Balázs AU - Török, Tibor AU - Williams, Michael J AU - Kurucz, Judit Éva AU - Andó, István TI - Identification of reference markers for characterizing honey bee (Apis mellifera) hemocyte classes JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 109 PY - 2020 SP - 103701 PG - 5 SN - 0145-305X DO - 10.1016/j.dci.2020.103701 UR - https://m2.mtmt.hu/api/publication/31302837 ID - 31302837 N1 - The publication is supported by the EFOP-3.6.3-VEKOP-16-2017-00008 project. The project is co-financed by the European Union and the European Social Fund. Immunology Unit, Institute of Genetics, Biological Research Centre, P.O.Box 521, Szeged, H-6701, Hungary University of Veterinary Medicine, István u. 2., Budapest, 1078, Hungary Department of Plant Science and Biotechnology, University of Pannonia, Georgikon Faculty, Deák F. u. 16., Keszthely, 8360, Hungary Department of Genetics, University of Szeged, Közép Fasor 52, Szeged, 6726, Hungary Functional Pharmacology, Department of Neuroscience, Uppsala University, Husargatan 3, Box 593, Uppsala, 751 24, Sweden Export Date: 10 December 2020 CODEN: DCIMD Correspondence Address: Andó, I.; Immunology Unit, Institute of Genetics, Biological Research Centre, P.O.Box 521, Hungary; email: ando@brc.hu AB - Cell mediated immunity of the honey bee (Apis mellifera) involves the activity of several hemocyte populations, currently defined by morphological features and lectin binding characteristics. The objective of the present study was to identify molecular markers capable of characterizing subsets of honey bee hemocytes. We developed and employed monoclonal antibodies with restricted reactions to functionally distinct hemocyte subpopulations. Melanizing cells, known as oenocytoids, were defined by an antibody to prophenoloxidase, aggregating cells were identified by the expression of Hemolectin, and phagocytic cells were identified by a marker expressed on granulocytes. We anticipate that this combination of antibodies not only allows for the detection of functionally distinct hemocyte subtypes, but will help to further the exploration of hematopoietic compartments, as well as reveal details of the honey bee cellular immune defense against parasites and microbes. LA - English DB - MTMT ER - TY - JOUR AU - Gábor, Erika AU - Cinege, Gyöngyi Ilona AU - Csordas, G AU - Honti, Viktor AU - Torok, T AU - Medzihradszky, KF AU - Darula, Z AU - Kurucz, Judit Éva AU - Andó, István TI - The identification of two important proteins in honey bee (Apis mellifera) cellular immunity JF - IMMUNOLÓGIAI SZEMLE J2 - IMMUNOLÓGIAI SZEMLE VL - 11 PY - 2019 IS - 3 SP - 12 SN - 2061-0203 UR - https://m2.mtmt.hu/api/publication/30864526 ID - 30864526 LA - English DB - MTMT ER - TY - THES AU - Gábor, Erika TI - A mézelő méh sejt-közvetítette immunitása PB - Szegedi Tudományegyetem (SZTE) PY - 2019 SP - 82 DO - 10.14232/phd.9885 UR - https://m2.mtmt.hu/api/publication/30704382 ID - 30704382 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Gábor, Erika AU - Cinege, Gyöngyi Ilona AU - Csordas, G AU - Torok, T AU - Medzihradszky, KF AU - Darula, Z AU - Kurucz, Judit Éva AU - Andó, István TI - Monoclonal antibodies for the characterization of hemocyte subpopulations in the honey bee, Apis mellifera JF - IMMUNOLÓGIAI SZEMLE J2 - IMMUNOLÓGIAI SZEMLE VL - 10 PY - 2018 IS - 3 SP - 31 SN - 2061-0203 UR - https://m2.mtmt.hu/api/publication/30313455 ID - 30313455 LA - English DB - MTMT ER - TY - JOUR AU - Gábor, Erika AU - Cinege, Gyöngyi Ilona AU - Csordas, G AU - Torok, T AU - Medzihradszky, FK AU - Darula, Z AU - Kurucz, Judit Éva AU - Ando, I TI - Molecular definition of the major hemocyte subsets in the honey bee (Apis mellifera) JF - IMMUNOLÓGIAI SZEMLE J2 - IMMUNOLÓGIAI SZEMLE VL - 9 PY - 2017 IS - 3 SP - 32 EP - 33 PG - 2 SN - 2061-0203 UR - https://m2.mtmt.hu/api/publication/3421274 ID - 3421274 LA - English DB - MTMT ER -