@article{MTMT:33555087, title = {A Novel Method for Primary Blood Cell Culturing and Selection in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/33555087}, author = {Kúthy-Sutus, Enikő and Kharrat, Bayan and Gábor, Erika and Csordás, Gábor and Sinka, Rita and Honti, Viktor}, doi = {10.3390/cells12010024}, journal-iso = {CELLS-BASEL}, journal = {CELLS}, volume = {12}, unique-id = {33555087}, abstract = {The blood cells of the fruit fly Drosophila melanogaster show many similarities to their vertebrate counterparts, both in their functions and their differentiation. In the past decades, a wide palette of immunological and transgenic tools and methods have been developed to study hematopoiesis in the Drosophila larva. However, the in vivo observation of blood cells is technically restricted by the limited transparency of the body and the difficulty in keeping the organism alive during imaging. Here we describe an improved ex vivo culturing method that allows effective visualization and selection of live blood cells in primary cultures derived from Drosophila larvae. Our results show that cultured hemocytes accurately represent morphological and functional changes following immune challenges and in case of genetic alterations. Since cell culturing has hugely contributed to the understanding of the physiological properties of vertebrate blood cells, this method provides a versatile tool for studying Drosophila hemocyte differentiation and functions ex vivo.}, year = {2023}, eissn = {2073-4409}, orcid-numbers = {Kúthy-Sutus, Enikő/0000-0002-1398-4120; Csordás, Gábor/0000-0001-6871-6839; Sinka, Rita/0000-0003-4040-4184} } @CONFERENCE{MTMT:34689202, title = {Studying Drosophila blood cell plasticity using primary blood cell cultures}, url = {https://m2.mtmt.hu/api/publication/34689202}, author = {Kúthy-Sutus, Enikő and Gábor, Erika and Kharrat, Bayan and Jankovics, Ferenc and Sinka, Rita and Honti, Viktor}, booktitle = {Straub-Napok 2022}, unique-id = {34689202}, year = {2022}, pages = {1}, orcid-numbers = {Kúthy-Sutus, Enikő/0000-0002-1398-4120; Sinka, Rita/0000-0003-4040-4184} } @article{MTMT:31743832, title = {There and back again: The mechanisms of differentiation and transdifferentiation in Drosophila blood cells}, url = {https://m2.mtmt.hu/api/publication/31743832}, author = {Csordás, Gábor and Gábor, Erika and Honti, Viktor}, doi = {10.1016/j.ydbio.2020.10.006}, journal-iso = {DEV BIOL}, journal = {DEVELOPMENTAL BIOLOGY}, volume = {469}, unique-id = {31743832}, issn = {0012-1606}, year = {2021}, eissn = {1095-564X}, pages = {135-143}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839} } @article{MTMT:31891672, title = {Function and Interacting Partners of Headcase in Drosophila Melanogaster}, url = {https://m2.mtmt.hu/api/publication/31891672}, author = {Kharrat, B and Gábor, Erika and Sinka, Rita and Honti, Viktor}, journal-iso = {IMMUNOLÓGIAI SZEMLE}, journal = {IMMUNOLÓGIAI SZEMLE}, volume = {12}, unique-id = {31891672}, issn = {2061-0203}, year = {2020}, pages = {25-25}, orcid-numbers = {Sinka, Rita/0000-0003-4040-4184} } @article{MTMT:31891648, title = {Vérsejtek transzdifferenciálódásának vizsgálata a mélytanulás módszerével Drosophila melanogasterben}, url = {https://m2.mtmt.hu/api/publication/31891648}, author = {Gábor, Erika and Varga, GI and Géczi, Aliz and Bayan, K and Migh, E and Beleon, A and Horvath, P and Szeplaki, B and Enyedi, M and Pinter, L and Haracska, L and Honti, Viktor}, journal-iso = {IMMUNOLÓGIAI SZEMLE}, journal = {IMMUNOLÓGIAI SZEMLE}, volume = {12}, unique-id = {31891648}, issn = {2061-0203}, year = {2020}, pages = {8-9} } @article{MTMT:31302837, title = {Identification of reference markers for characterizing honey bee (Apis mellifera) hemocyte classes}, url = {https://m2.mtmt.hu/api/publication/31302837}, author = {Gábor, Erika and Cinege, Gyöngyi Ilona and Csordás, Gábor and Rusvai, Miklós and Honti, Viktor and Kolics, Balázs and Török, Tibor and Williams, Michael J and Kurucz, Judit Éva and Andó, István}, doi = {10.1016/j.dci.2020.103701}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {109}, unique-id = {31302837}, issn = {0145-305X}, abstract = {Cell mediated immunity of the honey bee (Apis mellifera) involves the activity of several hemocyte populations, currently defined by morphological features and lectin binding characteristics. The objective of the present study was to identify molecular markers capable of characterizing subsets of honey bee hemocytes. We developed and employed monoclonal antibodies with restricted reactions to functionally distinct hemocyte subpopulations. Melanizing cells, known as oenocytoids, were defined by an antibody to prophenoloxidase, aggregating cells were identified by the expression of Hemolectin, and phagocytic cells were identified by a marker expressed on granulocytes. We anticipate that this combination of antibodies not only allows for the detection of functionally distinct hemocyte subtypes, but will help to further the exploration of hematopoietic compartments, as well as reveal details of the honey bee cellular immune defense against parasites and microbes.}, keywords = {Immunity; monoclonal antibody; Hemocyte; Apis mellifera; Honey bee; insect immunity}, year = {2020}, eissn = {1879-0089}, pages = {103701-103706}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Török, Tibor/0000-0002-2128-1126; Andó, István/0000-0002-4648-9396} } @article{MTMT:30864526, title = {The identification of two important proteins in honey bee (Apis mellifera) cellular immunity}, url = {https://m2.mtmt.hu/api/publication/30864526}, author = {Gábor, Erika and Cinege, Gyöngyi Ilona and Csordas, G and Honti, Viktor and Torok, T and Medzihradszky, KF and Darula, Z and Kurucz, Judit Éva and Andó, István}, journal-iso = {IMMUNOLÓGIAI SZEMLE}, journal = {IMMUNOLÓGIAI SZEMLE}, volume = {11}, unique-id = {30864526}, issn = {2061-0203}, year = {2019}, pages = {12}, orcid-numbers = {Andó, István/0000-0002-4648-9396} } @mastersthesis{MTMT:30704382, title = {A mézelő méh sejt-közvetítette immunitása}, url = {https://m2.mtmt.hu/api/publication/30704382}, author = {Gábor, Erika}, doi = {10.14232/phd.9885}, publisher = {SZTE}, unique-id = {30704382}, year = {2019} } @article{MTMT:30313455, title = {Monoclonal antibodies for the characterization of hemocyte subpopulations in the honey bee, Apis mellifera}, url = {https://m2.mtmt.hu/api/publication/30313455}, author = {Gábor, Erika and Cinege, Gyöngyi Ilona and Csordas, G and Torok, T and Medzihradszky, KF and Darula, Z and Kurucz, Judit Éva and Andó, István}, journal-iso = {IMMUNOLÓGIAI SZEMLE}, journal = {IMMUNOLÓGIAI SZEMLE}, volume = {10}, unique-id = {30313455}, issn = {2061-0203}, year = {2018}, pages = {31}, orcid-numbers = {Andó, István/0000-0002-4648-9396} } @article{MTMT:3421274, title = {Molecular definition of the major hemocyte subsets in the honey bee (Apis mellifera)}, url = {https://m2.mtmt.hu/api/publication/3421274}, author = {Gábor, Erika and Cinege, Gyöngyi Ilona and Csordas, G and Torok, T and Medzihradszky, FK and Darula, Z and Kurucz, Judit Éva and Ando, I}, journal-iso = {IMMUNOLÓGIAI SZEMLE}, journal = {IMMUNOLÓGIAI SZEMLE}, volume = {9}, unique-id = {3421274}, issn = {2061-0203}, year = {2017}, pages = {32-33} }