TY - JOUR AU - Kontra, Bence AU - Mucsi, Zoltán AU - Vanyorek, László AU - Nagy, Miklós TI - Optical Investigation of 2-amino-7-isocyanofluorene, a Novel Blue-Emitting Solvatochromic Dye JF - COLORANTS J2 - Colorants VL - 3 PY - 2024 IS - 2 SP - 86 EP - 98 PG - 13 SN - 2079-6447 DO - 10.3390/colorants3020006 UR - https://m2.mtmt.hu/api/publication/34757551 ID - 34757551 AB - Smart solvatochromic isocyano-aminoarenes (ICAArs) have been gaining attention owing to their unique photophysical, antifungal and anticancer properties. Using a simple dehydration reaction with in situ-generated dichlorocarbene, we prepared 2-amino-7-isocyanofluorene (2,7-ICAF). We studied the effect of the longer polarization axis provided by the fluorene core on the spectral properties and we also compared it to those of the starting diamine. 2,7-ICAF shows a clear solvatochromic behavior close to the blue part (370–420 nm) of the visible spectrum. Quantum chemical calculations show internal charge transfer (ICT) between the donor amino and the electron-withdrawing isocyano groups. 2,7-ICAF has high molar absorptivity (ε = 15–18·103 M−1cm−1) and excellent quantum yield (Φf = 70–95%) in most solvents; however, its fluorescence is completely quenched in water. The high brightness (ε·Φf) and close to zero quantum yield in water may be favorable in biolabeling applications, where background fluorescence should be kept minimal. Overall, 2,7-ICAF shows enhanced photophysical properties compared to its previously investigated relative 4-amino-4′-isocyano-1,1′-biphenyl (4,4′-ICAB). LA - English DB - MTMT ER - TY - JOUR AU - Csomos, Attila AU - Madarász, Miklós AU - Turczel, Gábor AU - Cseri, Levente AU - Katona, Gergely AU - Rózsa J., Balázs AU - Kovács, Ervin AU - Mucsi, Zoltán TI - A Molecular Hybrid of the GFP Chromophore and 2,2′-Bipyridine: An Accessible Sensor for Zn2+ Detection with Fluorescence Microscopy JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 6 PG - 14 SN - 1661-6596 DO - 10.3390/ijms25063504 UR - https://m2.mtmt.hu/api/publication/34748236 ID - 34748236 N1 - Department of Chemistry, Femtonics Ltd, Tűzoltó utca 59, Budapest, H-1094, Hungary Hevesy György PhD School of Chemistry, Eötvös Loránd University, Pázmány Péter sétány 1/A, Budapest, H-1117, Hungary BrainVisionCenter, Liliom utca 43, Budapest, H-1094, Hungary NMR Research Laboratory, HUN-REN Research Centre for Natural Sciences, Magyar Tudósok körútja 2, Budapest, H-1117, Hungary Department of Organic Chemistry & Technology, Budapest University of Technology & Economics, 3. Muegyetem rakpart, Budapest, H-1111, Hungary Two-Photon Measurement Technology Research Group, Pázmány Péter Catholic University, Práter utca 50/a, Budapest, H-1083, Hungary Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Szigony utca 43, Budapest, H-1083, Hungary Polymer Chemistry and Physics Research Group, HUN-REN Research Centre for Natural Sciences, Magyar Tudósok körútja 2, Budapest, H-1117, Hungary Faculty of Materials and Chemical Sciences, University of Miskolc, Miskolc, H-3515, Hungary Export Date: 12 April 2024 Correspondence Address: Rózsa, B.; BrainVisionCenter, Liliom utca 43, Hungary; email: rozsabal@koki.hu Correspondence Address: Kovács, E.; Two-Photon Measurement Technology Research Group, Práter utca 50/a, Hungary; email: kovacs.ervin@ttk.hu AB - The few commercially available chemosensors and published probes for in vitro Zn2+ detection in two-photon microscopy are compromised by their flawed spectroscopic properties, causing issues in selectivity or challenging multistep syntheses. Herein, we present the development of an effective small molecular GFP chromophore-based fluorescent chemosensor with a 2,2′-bipyridine chelator moiety (GFZnP BIPY) for Zn2+ detection that has straightforward synthesis and uncompromised properties. Detailed experimental characterizations of the free and the zinc-bound compounds within the physiologically relevant pH range are presented. Excellent photophysical characteristics are reported, including a 53-fold fluorescence enhancement with excitation and emission maxima at 422 nm and 492 nm, respectively. A high two-photon cross section of 3.0 GM at 840 nm as well as excellent metal ion selectivity are reported. In vitro experiments on HEK 293 cell culture were carried out using two-photon microscopy to demonstrate the applicability of the novel sensor for zinc bioimaging. LA - English DB - MTMT ER - TY - JOUR AU - Csomos, Attila AU - Madarász, Miklós AU - Turczel, Gábor AU - Cseri, Levente AU - Bodor, Andrea AU - Anett, Matuscsák AU - Katona, Gergely AU - Kovács, Ervin AU - Rózsa J., Balázs AU - Mucsi, Zoltán TI - A GFP inspired 8-methoxyquinoline-derived fluorescent molecular sensor for the detection of Zn2+ by two-photon microscopy JF - CHEMISTRY-A EUROPEAN JOURNAL J2 - CHEM-EUR J PY - 2024 SN - 0947-6539 DO - 10.1002/chem.202400009 UR - https://m2.mtmt.hu/api/publication/34724516 ID - 34724516 AB - An effective, GFP-inspired fluorescent Zn2+ sensor is developed for two-photon microscopy and related biological application that featured an 8-methoxyquinoline moiety. Excellent photophysical characteristics including a 37-fold fluorescence enhancement with excitation and emission maxima at 440 nm and 505 nm, respectively, as well as a high two-photon cross-section of 73 GM at 880 nm are reported. Based on the experimental data, the relationship between the structure and properties was elucidated and explained backed up by DFT calculations, particularly to the observed PeT phenomenon for the turn-on process. Biological validation and detailed experimental and theoretical characterization of the free and the zinc-bound compounds are presented. LA - English DB - MTMT ER - TY - PAT AU - Csomos, Attila AU - Kovács, Ervin AU - Barkai, Gyöngy AU - Váradi, Csaba AU - Mucsi, Zoltán AU - Viskolcz, Béla AU - Rózsa J., Balázs AU - Cseri, Levente AU - Dojcsák, Dalma TI - Glikomikai alkalmazásra fejlesztett permanens töltéssel rendelkező fluoreszcens jelölővegyületek CY - Country:10001(1) PY - 2024 UR - https://m2.mtmt.hu/api/publication/34561430 ID - 34561430 AB - A bejelentés új, glikoproteinek elemzésére alkalmazható új jelölővegyületekre és kimutatási eljárásokra vonatkozik. Az új vegyületek N-hidroxi-szukcinimidil-karbamáttal származékolt xanténvázas rodamin- vagy pironin-származékok, amelyek N-glikánok folyadék-kromatográfiás elválasztást követő fluoreszcens és tömegspektrometriás detektálására használhatók fel. LA - Hungarian DB - MTMT ER - TY - PAT AU - Domahidy, Farkas László AU - Kovács, Ervin AU - Mucsi, Zoltán AU - Cseri, Levente AU - Rózsa J., Balázs TI - Piránvázas fluoreszcens jelzővegyületek DNS kimutatására CY - Country:10001(1) PY - 2024 UR - https://m2.mtmt.hu/api/publication/34561278 ID - 34561278 AB - Találmányunk tárgyát képezik új piránvázas fluoreszcens jelzővegyületek, amely töltéssel rendelkező pirángyűrűn 2 és 6 helyzetben kapcsolódó fenil csoportokhoz amino csoportok kapcsolódnak, amelyek nitrogénjei olyan 2-3 szénatomos lánccal vannak szubsztituálva, amelyek szekunder vagy tercier aminocsoportot tartalmaznak. Ezen szekunder amino csoportok hatására a találmányunk szerinti színezék a korábban ismertnél előnyösebb, jobb vízoldhatóságú, a DNS-hez jól kötődő és DNS jelenlétében meglepően jobb fluoreszcencia-kvantumhatásfokú színezékek. LA - Hungarian DB - MTMT ER - TY - JOUR AU - Csomos, Attila AU - Kovács, Ervin AU - Madarász, Miklós AU - Fedor, Flóra Zsófia AU - Fülöp, Anna AU - Katona, Gergely AU - Rózsa J., Balázs AU - Mucsi, Zoltán TI - Two-Photon Fluorescent Chemosensors Based on the GFP-Chromophore for the Detection of Zn2+ in Biological Samples – From Design to Application JF - SENSORS AND ACTUATORS B-CHEMICAL J2 - SENSOR ACTUAT B CHEM VL - 398 PY - 2024 PG - 14 SN - 0925-4005 DO - 10.1016/j.snb.2023.134753 UR - https://m2.mtmt.hu/api/publication/34191513 ID - 34191513 LA - English DB - MTMT ER - TY - CHAP AU - Rózsa J., Balázs AU - Szadai, Zoltán AU - Judák, Linda AU - Chiovini, Balázs AU - Juhász, Gábor AU - Ócsai, Katalin AU - Pálfi, Dénes AU - Mezriczky, Zsolt AU - Szalay, Gergely AU - Katona, Gergely AU - Mihály, Anna AU - Szepesi, Áron AU - Mucsi, Zoltán AU - Roska, Botond AU - Pi, Hyun-Jae AU - Chevy, Quentin AU - Albeanu, Florin AU - Kepecs, Adam TI - Imaging of dendrites and sparse interneuronal networks with 3D random access microscopy T2 - Optics and the Brain part of Optica Biophotonics Congress: Optics in the Life Sciences, 2023 PB - Optica Publishing Group CY - Washington DC SN - 9781957171210 T3 - Technical digest series / Optica Publishing Group PY - 2023 SP - 2023 DO - 10.1364/BRAIN.2023.BW3B.6 UR - https://m2.mtmt.hu/api/publication/34753229 ID - 34753229 AB - Acousto-optical microscopy is a powerful tool to study spare networks and extensive dendritic arborization from the cortex of behaving animals. We used this novel approach for imaging dendrites and somata of sparse interneuron populations in a combination with auditory discrimination and detection tasks. Our results shed light of not yet known subcellular and network mechanisms from multiple brain regions. LA - English DB - MTMT ER - TY - PAT AU - Nagy, Miklós AU - Vanyorek, László AU - Braun, Mihály AU - Sóvágó, Dávid AU - Mucsi, Zoltán AU - Viskolcz, Béla AU - Czébely, Andrea TI - Eljárás higany hatékony eltávolítására vízből aromás izocianidok felhasználásával redukálószerként CY - Country:10001(1) PY - 2023 UR - https://m2.mtmt.hu/api/publication/34511143 ID - 34511143 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Kopcsik, Erika AU - Mucsi, Zoltán AU - Kontra, Bence AU - Vanyorek, László AU - Gál, R. AU - Viskolcz, Béla AU - Nagy, Miklós TI - Excited state iminium form can explain the unexpected solvatochromic behavior of symmetric 1,5- and 1,8-diaminonaphthalenes JF - CHEMICAL COMMUNICATIONS J2 - CHEM COMMUN VL - 60 PY - 2023 IS - 8 SP - 1008 EP - 1011 PG - 4 SN - 1359-7345 DO - 10.1039/d3cc05643k UR - https://m2.mtmt.hu/api/publication/34509253 ID - 34509253 LA - English DB - MTMT ER - TY - JOUR AU - Kopcsik, Erika AU - Mucsi, Zoltán AU - Nagy, Miklós TI - Az 1,5-diizocianonaftalin optikai tulajdonságainak vizsgálata JF - Doktorandusz Almanach J2 - Doktorandusz Almanach VL - 1 PY - 2023 SP - 117 EP - 122 PG - 6 SN - 2939-7294 UR - https://m2.mtmt.hu/api/publication/34442783 ID - 34442783 LA - Hungarian DB - MTMT ER -