TY - JOUR AU - Csorba, Anita AU - Katona, Gábor AU - Budai-Szűcs, Mária AU - Balogh Weiser, Diána AU - Molnár, P. AU - Maka, Erika AU - Nochta-Kazsoki, Adrienn Katalin AU - Vajna, Márton Antal AU - Zelkó, Romána AU - Nagy, Zoltán Zsolt AU - Balogh, György Tibor TI - A Comparative Pharmacokinetic Study for Cysteamine-Containing Eye Drops as an Orphan Topical Therapy in Cystinosis JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 3 PG - 14 SN - 1661-6596 DO - 10.3390/ijms25031623 UR - https://m2.mtmt.hu/api/publication/34538437 ID - 34538437 N1 - Department of Ophthalmology, Semmelweis University, Mária Street 39, Budapest, H-1085, Hungary Institute of Pharmaceutical Technology and Regulatory Affairs, Faculty of Pharmacy, University of Szeged, Eötvös Street 6, Szeged, H-6720, Hungary Department of Physical Chemistry and Materials Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Molteam Llc., Mélyfúró Street 4, Budapest, H-1151, Hungary University Pharmacy Department of Pharmacy Administration, Semmelweis University, Hőgyes Endre Street 7-9, Budapest, H-1092, Hungary Department of Pharmaceutical Chemistry, Semmelweis University, Hőgyes Endre Street 7-9, Budapest, H-1092, Hungary Export Date: 23 February 2024 Correspondence Address: Csorba, A.; Department of Ophthalmology, Mária Street 39, Hungary; email: csorba.anita@semmelweis-univ.hu Correspondence Address: Balogh, G.T.; Department of Pharmaceutical Chemistry, Hőgyes Endre Street 7-9, Hungary; email: balogh.gyorgy.tibor@semmelweis.hu LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Gergő Dániel AU - Koplányi, Gábor AU - Kenéz, Balázs AU - Balogh Weiser, Diána TI - Nanoformulation of Therapeutic Enzymes: A Short Review JF - PERIODICA POLYTECHNICA-CHEMICAL ENGINEERING J2 - PERIOD POLYTECH CHEM ENG VL - 67 PY - 2023 IS - 4 SP - 624 EP - 635 PG - 12 SN - 0324-5853 DO - 10.3311/PPch.22826 UR - https://m2.mtmt.hu/api/publication/34155138 ID - 34155138 N1 - Department of Physical Chemistry and Materials Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Department of Organic Chemistry Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Export Date: 30 November 2023 Correspondence Address: Balogh-Weiser, D.; Department of Physical Chemistry and Materials Science, Műegyetem rkp. 3, Hungary; email: balogh.weiser.diana@vbk.bme.hu AB - Enzyme replacement therapy (ERT) is a therapeutic approach that involves the administration of specific enzymes to the patient in order to correct metabolic defects caused by enzyme deficiency. The formulation of ERTs involves the production, purification, and formulation of the enzyme into a stable and biologically active drug product, often using recombinant DNA technology. Non-systemic ERTs often involve the immobilization of the enzyme on a carrier, such as hydrogels, liposomes, or nanoparticles. ERT holds great promise for the treatment of a wide range of genetic disorders, and its success regarding lysosomal storage diseases, such as Fabry disease, Gaucher disease, and Pompe disease has paved the way for the development of similar therapies for other genetic disorders too. LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Gergő D. AU - Kállai-Szabó, Nikolett AU - Lengyel, Miléna AU - Süvegh, Károly AU - Ender, Ferenc AU - Katona, Gábor AU - Nochta-Kazsoki, Adrienn Katalin AU - Zelkó, Romána AU - Antal, István AU - Balogh, György Tibor AU - Balogh Weiser, Diána TI - Nanoformulation of lipase from Porcine pancreas by electrospinning as a novel alternative for enzyme-based per os therapies JF - JOURNAL OF MOLECULAR LIQUIDS J2 - J MOL LIQ VL - 389 PY - 2023 PG - 13 SN - 0167-7322 DO - 10.1016/j.molliq.2023.122819 UR - https://m2.mtmt.hu/api/publication/34096036 ID - 34096036 N1 - Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Department of Pharmaceutics, Semmelweis University, Hőgyes E. Street 7–9, Budapest, H-1092, Hungary Eötvös Loránd University Laboratory of Nuclear Chemistry, P.O. Box 32, Budapest, H-1518, Hungary Department of Electron Devices, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Spinsplit Llc., Vend u. 17, Budapest, H-1025, Hungary Institute of Pharmaceutical Technology and Regulatory Affairs, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, Szeged, H-6720, Hungary University Pharmacy, Department of Pharmacy Administration, Faculty of Pharmaceutical Sciences, Semmelweis University, Hőgyes E. Street 7–9, Budapest, H-1092, Hungary Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Semmelweis University, Hőgyes E. Street 7–9, Budapest, H-1092, Hungary Department of Chemical and Environmental Process Engineering, Budapest University of Technology and Economics, Budapest, H-1111, Hungary Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Export Date: 20 February 2024 CODEN: JMLID Correspondence Address: Antal, I.; Department of Pharmaceutical Chemistry, Hőgyes E. Street 7–9, Hungary; email: antal.istvan@semmelweis.hu LA - English DB - MTMT ER - TY - JOUR AU - Balogh Weiser, Diána AU - Molnár, Alexandra AU - Tóth, Gergő Dániel AU - Koplányi, Gábor AU - Szemes, József AU - Decsi, Balázs AU - Katona, Gábor AU - Salamah, Maryana AU - Ender, Ferenc AU - Kovács, Anita AU - Berkó, Szilvia AU - Budai-Szűcs, Mária AU - Balogh, György Tibor TI - Combined Nanofibrous Face Mask: Co-Formulation of Lipases and Antibiotic Agent by Electrospinning Technique JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 15 PY - 2023 IS - 4 PG - 19 SN - 1999-4923 DO - 10.3390/pharmaceutics15041174 UR - https://m2.mtmt.hu/api/publication/33740967 ID - 33740967 N1 - Funding Agency and Grant Number: National Research Development and Innovation (NRDI) Fund [PD-131467]; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences [BO/00175/21]; New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund [UNKP-22-3-1-BME-173]; Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund [TKP-9-8/PALY-2021]; European Union [RRF-2.3.1-21-2022-00015] Funding text: This research has been supported by the National Research Development and Innovation (NRDI) Fund via grant PD-131467 (D.B.W.). B.W.D. acknowledges the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/00175/21). The study was supported by the UNKP-22-3-1-BME-173 New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund. The research was performed in the frame of Project no. TKP-9-8/PALY-2021 has been implemented with the support provided by the Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund, financed under the TKP2021-EGA funding scheme and Project no. RRF-2.3.1-21-2022-00015 has been implemented with the support provided by the European Union. AB - The application of enzyme-based therapies has received significant attention in modern drug development. Lipases are one of the most versatile enzymes that can be used as therapeutic agents in basic skin care and medical treatment related to excessive sebum production, acne, and inflammation. The traditional formulations available for skin treatment, such as creams, ointments or gels, are widely applied; however, their use is not always accompanied by good drug penetration properties, stability, or patient adherence. Nanoformulated drugs offer the possibility of combining enzymatic and small molecule formulations, making them a new and exciting alternative in this field. In this study polymeric nanofibrous matrices made of polyvinylpyrrolidone and polylactic acid were developed, entrapping lipases from Candida rugosa and Rizomucor miehei and antibiotic compound nadifloxacin. The effect of the type of polymers and lipases were investigated, and the nanofiber formation process was optimized to provide a promising alternative in topical treatment. Our experiments have shown that entrapment by electrospinning induced two orders of magnitude increase in the specific enzyme activity of lipases. Permeability investigations indicated that all lipase-loaded nanofibrous masks were capable of delivering nadifloxacin to the human epidermis, confirming the viability of electrospinning as a formulation method for topical skin medications. LA - English DB - MTMT ER - TY - JOUR AU - Vincze, Anna AU - Dékány, Gergely AU - Bicsak, Richárd AU - Formanek, András AU - Moreau, Yves AU - Koplányi, Gábor AU - Takács, Gergely AU - Katona, Gábor AU - Balogh Weiser, Diána AU - Arany, Ádám AU - Balogh, György Tibor TI - Natural Lipid Extracts as an Artificial Membrane for Drug Permeability Assay: In Vitro and In Silico Characterization JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 15 PY - 2023 IS - 3 PG - 19 SN - 1999-4923 DO - 10.3390/pharmaceutics15030899 UR - https://m2.mtmt.hu/api/publication/33692858 ID - 33692858 N1 - Funding Agency and Grant Number: Richter Gedeon Excellence PhD Scholarship from the Richter Gedeon Foundation [BO/00175/21]; Hungarian Academy of Sciences Funding text: This project was supported by the Central Europe Leuven Strategic Alliance (CELSA-2021/019) and by an FK-137582 grant from the National Research and Innovation Office, Hungary (NRDI). AB - In vitro non-cellular permeability models such as the parallel artificial membrane permeability assay (PAMPA) are widely applied tools for early-phase drug candidate screening. In addition to the commonly used porcine brain polar lipid extract for modeling the blood–brain barrier’s permeability, the total and polar fractions of bovine heart and liver lipid extracts were investigated in the PAMPA model by measuring the permeability of 32 diverse drugs. The zeta potential of the lipid extracts and the net charge of their glycerophospholipid components were also determined. Physicochemical parameters of the 32 compounds were calculated using three independent forms of software (Marvin Sketch, RDKit, and ACD/Percepta). The relationship between the lipid-specific permeabilities and the physicochemical descriptors of the compounds was investigated using linear correlation, Spearman correlation, and PCA analysis. While the results showed only subtle differences between total and polar lipids, permeability through liver lipids highly differed from that of the heart or brain lipid-based models. Correlations between the in silico descriptors (e.g., number of amide bonds, heteroatoms, and aromatic heterocycles, accessible surface area, and H-bond acceptor–donor balance) of drug molecules and permeability values were also found, which provides support for understanding tissue-specific permeability. LA - English DB - MTMT ER - TY - JOUR AU - Csorba, Anita AU - Katona, Gábor AU - Budai-Szűcs, Mária AU - Balogh Weiser, Diána AU - Fadda, Anna Maria AU - Caddeo, Carla AU - Takács, Ágnes Ildikó AU - Mátyus, Péter AU - Balogh, György Tibor AU - Nagy, Zoltán Zsolt TI - Effect of liposomal formulation of ascorbic acid on corneal permeability JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 13 PY - 2023 IS - 1 PG - 12 SN - 2045-2322 DO - 10.1038/s41598-023-29290-9 UR - https://m2.mtmt.hu/api/publication/33675955 ID - 33675955 N1 - Funding Agency and Grant Number: Semmelweis University Funding text: Open access funding provided by Semmelweis University. AB - Ascorbic acid (AA) has a pivotal role in corneal wound healing via stimulating the biosynthesis of highly organized extracellular matrix components, but its rapid degradation and low corneal permeability limits its therapeutic effects. In this paper, we present the pharmacokinetic properties of a liposomal-based formulation of AA in terms of corneal permeation. Chemical stability, shelf-life, and drug release rate of lyophilized liposome (AA-LLipo) formulation was determined in comparison to free-form of AA solution using high-performance liquid chromatography (HPLC) and rapid equilibrium dialysis. In vitro transcorneal permeability was studied using a parallel artificial membrane permeability assay (PAMPA). Ex vivo permeation was examined on AA-LLipo-treated porcine cornea by determining the AA content on the ocular surface, in the cornea as well as in the aqueous humor using HPLC, and by Raman-mapping visualizing the AA-distribution. Our results showed that the liposomal formulation improved the chemical stability of AA, while drug release was observed with the same kinetic efficiency as from the free-form of AA solution. Both corneal-PAMPA and porcine corneal permeability studies showed that AA-LLipo markedly improved the corneal absorption kinetics of AA, thus, increasing the AA content in the cornea and aqueous humor. AA-LLipo formulation could potentially increase the bioavailability of AA in corneal tissues. LA - English DB - MTMT ER - TY - JOUR AU - Imarah, Ali O. AU - Silva, Fausto M. W. G. AU - Bataa, Naran AU - Decsi, Balázs AU - Balogh Weiser, Diána AU - Poppe, László TI - Magnetically agitated continuous-flow tube reactors with aspartate ammonia-lyase immobilized on magnetic nanoparticles JF - REACTION CHEMISTRY & ENGINEERING J2 - REACT CHEM ENG VL - 8 PY - 2023 IS - 6 SP - 1250 EP - 1259 PG - 10 SN - 2058-9883 DO - 10.1039/D2RE00507G UR - https://m2.mtmt.hu/api/publication/33620537 ID - 33620537 N1 - Funding Agency and Grant Number: Ministry for Innovation and Technology of Hungary from the National Research, Development and Innovation Fund (NRDI) under the TKP2021 funding scheme; Romanian Ministry of Education and Research, CCCDI - UEFISCDI , within PNCDI III; Stipendium Hungaricum Scholarship Program; NRDI [BO/00175/21]; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences; [FK-137582]; [TKP2021-EGA-02]; [PN-III-P2-2.1-PED-2019-5031] Funding text: The research reported in this paper is part of project no. TKP2021-EGA-02, implemented with the support provided by the Ministry for Innovation and Technology of Hungary from the National Research, Development and Innovation Fund (NRDI), financed under the TKP2021 funding scheme. This work was also supported by a grant of the Romanian Ministry of Education and Research, CCCDI - UEFISCDI, project number PN-III-P2-2.1-PED-2019-5031, within PNCDI III. A. O. I. and F. M. G. W. S. acknowledge the Stipendium Hungaricum Scholarship Program for supporting the research work and their Ph.D. study. D. B.-W. acknowledges the grant FK-137582 provided by NRDI and the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/00175/21). We thank Gabor Koplanyi (Budapest, Hungary) for characterization of the MNP preparations by DLS and ?-potential measurements. AB - Two magnetically agitated continuous-flow tube reactors (AFRs)—applying external permanent magnets to move magnetic nanoparticles coated with a biocatalyst within a stream of the reaction medium—were developed and studied with aspartate ammonia-lyase (AAL) from Pseudomonas fluorescens immobilized onto epoxy-functionalized magnetic nanoparticles (MNPs) by covalent binding. The biotransformation of L-aspartate to fumarate by the AAL-MNPs (5 mg, D = 420 nm, 6 μg g−1 AAL) took place in the reaction tube (PTFE, ID 2.15 mm) of both AFRs in a space containing the AAL-MNPs agitated within the flow of the reaction medium (0.5 mM L-aspartate solution, 4.7–14 μL min−1, 25 °C) by two permanent ring magnets (N48 neodymium, 10 × 5 × 5 mm) positioned at a fixed distance in attraction mode. In the first version (AFRXM), the two magnets positioned at opposite sides of the reaction tube (distance: 20 mm) performed axial movement (amplitude: 8 mm, frequency: 40–140 mpm) along the Y-axis, being perpendicular to the X-axis of the tube. In the second version (AFRRM), the two magnets (distance: 10 mm from each other, 5 mm from the X-axis) performed rotation movement (frequency: 40–140 rpm) around the X-axis. Whereas in the AFRXM the AAL-MNPs formed a cloud moving back and forth, they created a ring-shaped cloud rotating within the tube in the AFRRM. The efficient internal mixing in the AFRRM at the best frequency (80 rpm) resulted in the highest apparent specific activity (Ub = 354–469 U g−1, at residence times of 2.5–7.5 min) of the AAL-MNPs in the reactors studied. In the other continuous-flow systems, significantly lower Ub values were achieved (135–290 U g−1 at 120 mpm in AFRXM; or 142–273 U g−1 and 64–129 U g−1 in tubular reactors anchoring MNPs in static mode with double or single magnets, respectively), whereas more than a magnitude of order lower values could be realised in the batch mode reactors (11.4–14.9 U g−1 with rotational magnetic agitation at 120 rpm; 5.0–5.8 U g−1 with axial magnetic agitation at 160 mpm; or 4.6–5.2 U g−1 in an orbital shaker at 600 rpm) at comparable reaction times (2.5–7.5 min). LA - English DB - MTMT ER - TY - JOUR AU - Koplányi, Gábor AU - Bell, Evelin AU - Molnár, Zsófia Klára AU - Katona, Gábor AU - Neumann, Péter Lajos AU - Ender, Ferenc AU - Balogh, György Tibor AU - Žnidaršič-Plazl, Polona AU - Poppe, László AU - Balogh Weiser, Diána TI - Novel Approach for the Isolation and Immobilization of a Recombinant Transaminase. Applying an Advanced Nanocomposite System TS - Applying an Advanced Nanocomposite System JF - CHEMBIOCHEM J2 - CHEMBIOCHEM VL - 24 PY - 2023 IS - 7 PG - 11 SN - 1439-4227 DO - 10.1002/cbic.202200713 UR - https://m2.mtmt.hu/api/publication/33574611 ID - 33574611 LA - English DB - MTMT ER - TY - JOUR AU - Balogh, György Tibor AU - Decsi, Balázs AU - Krammer, Réka AU - Kenéz, Balázs AU - Ender, Ferenc AU - Hergert, Tamás AU - Balogh Weiser, Diána TI - Effect of Binding Linkers on the Efficiency and Metabolite Profile of Biomimetic Reactions Catalyzed by Immobilized Metalloporphyrin JF - METABOLITES J2 - METABOLITES VL - 12 PY - 2022 IS - 12 SP - 1269 PG - 13 SN - 2218-1989 DO - 10.3390/metabo12121269 UR - https://m2.mtmt.hu/api/publication/33363235 ID - 33363235 N1 - Export Date: 5 April 2023 Correspondence Address: Balogh, G.T.; Department of Chemical and Environmental Process Engineering, Műegyetem rkp. 3., Hungary; email: balogh.gyorgy@vbk.bme.hu Correspondence Address: Balogh-Weiser, D.; Department of Organic Chemistry and Technology, Műegyetem rkp. 3, Hungary; email: balogh.weiser.diana@vbk.bme.hu AB - The investigation of liver-related metabolic stability of a drug candidate is a widely used key strategy in early-stage drug discovery. Metalloporphyrin-based biomimetic catalysts are good and well-described models of the function of CyP450 in hepatocytes. In this research, the immobilization of an iron porphyrin was performed on nanoporous silica particles via ionic interactions. The effect of the metalloporphyrin binding linkers was investigated on the catalytic efficiency and the metabolic profile of chloroquine as a model drug. The length of the amino-substituted linkers affects the chloroquine conversion as well as the ratio of human major and minor metabolites. While testing the immobilized catalysts in the continuous-flow reactor, results showed that the presented biomimetic system could be a promising alternative for the early-stage investigation of drug metabolites regarding analytical or synthetic goals as well. LA - English DB - MTMT ER - TY - JOUR AU - Balogh Weiser, Diána AU - Poppe, László AU - Kenéz, Balázs AU - Decsi, Balázs AU - Koplányi, Gábor AU - Katona, Gábor AU - Gyarmati, Benjámin Sándor AU - Ender, Ferenc AU - Balogh, György Tibor TI - Novel biomimetic nanocomposite for investigation of drug metabolism JF - JOURNAL OF MOLECULAR LIQUIDS J2 - J MOL LIQ VL - 368 PY - 2022 IS - Part B PG - 10 SN - 0167-7322 DO - 10.1016/j.molliq.2022.120781 UR - https://m2.mtmt.hu/api/publication/33229099 ID - 33229099 N1 - Funding Agency and Grant Number: NRDI Fund (TKP2020 NC); Ministry for Innovation and Technology; New National Excellence Program of the Ministry of Human Capacities and OTKA PD grant [131467]; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences; New National Excellence Program of the Ministry of Human Capacities; OTKA PD grant by the NRDI Office; Servier-Beregi Foundation; [BME-NC]; [BO/00175/21]; [UNKP-21-5 (UNKP-21-5-BME-386)] Funding text: The research reported in this paper and carried out at BME has been supported by the NRDI Fund (TKP2020 NC, Grant No. BME-NC) based on the charter of bolster issued by the NRDI Office under the auspices of the Ministry for Innovation and Technology. D.B.W. acknowledges the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/00175/21) , the UNKP-21-5 (UNKP-21-5-BME-386) New National Excellence Program of the Ministry of Human Capacities and OTKA PD grant (131467) by the NRDI Office. B.D. thanks Servier-Beregi Foundation for the support of this research. Part number: B AB - In vitro mimicking of hepatic drug metabolism is a key issue in early-stage drug discovery. Synthetic metalloporphyrins show structural similarity with the heme type prosthetic group of cytochrome P450 as primary hepatic enzyme in oxidative drug biotransformation. Therefore, they can catalyze these oxidations. Concerning economical aspects and the poor stability of metalloporphyrin, their immobilization onto or into solid carriers can be promising solution. This study presents a novel immobilized metalloporphyrin nanocomposite system and its potential use as biomimetic catalysts. The developed two-step immobilization procedure consists of two main steps. First, the ionic binding of meso-tetra (parasulphonatophenyl) iron porphyrin onto functionalized magnetic nanoparticles is established, followed by embedding the nanoparticles into polylactic acid nanofibers by electrospinning technique. Due to the synergistic morphological and chemo-structural advantages of binding onto nanoparticles and embedding in polymeric matrices the biomimetic efficiency of metalloporphyrin can be remarkably enhanced, while substrate conversion value was tenfold larger than which could be achieved with classic human liver microsomal system. LA - English DB - MTMT ER -