@article{MTMT:34538437,
	title = {A Comparative Pharmacokinetic Study for Cysteamine-Containing Eye Drops as an Orphan Topical Therapy in Cystinosis},
	url = {https://m2.mtmt.hu/api/publication/34538437},
	author = {Csorba, Anita and Katona, Gábor and Budai-Szűcs, Mária and Balogh Weiser, Diána and Molnár, P. and Maka, Erika and Nochta-Kazsoki, Adrienn Katalin and Vajna, Márton Antal and Zelkó, Romána and Nagy, Zoltán Zsolt and Balogh, György Tibor},
	doi = {10.3390/ijms25031623},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {25},
	unique-id = {34538437},
	issn = {1661-6596},
	year = {2024},
	eissn = {1422-0067},
	orcid-numbers = {Csorba, Anita/0000-0002-3256-9440; Katona, Gábor/0000-0003-1564-4813; Budai-Szűcs, Mária/0000-0001-5187-5702; Balogh Weiser, Diána/0000-0002-9957-1203; Maka, Erika/0000-0002-3631-3506; Nochta-Kazsoki, Adrienn Katalin/0000-0002-0611-3124; Vajna, Márton Antal/0000-0001-5280-7533; Zelkó, Romána/0000-0002-5419-9137; Nagy, Zoltán Zsolt/0000-0002-7330-0464; Balogh, György Tibor/0000-0003-3347-1880}
}

@article{MTMT:34155138,
	title = {Nanoformulation of Therapeutic Enzymes: A Short Review},
	url = {https://m2.mtmt.hu/api/publication/34155138},
	author = {Tóth, Gergő Dániel and Koplányi, Gábor and Kenéz, Balázs and Balogh Weiser, Diána},
	doi = {10.3311/PPch.22826},
	journal-iso = {PERIOD POLYTECH CHEM ENG},
	journal = {PERIODICA POLYTECHNICA-CHEMICAL ENGINEERING},
	volume = {67},
	unique-id = {34155138},
	issn = {0324-5853},
	abstract = {Enzyme replacement therapy (ERT) is a therapeutic approach that involves the administration of specific enzymes to the patient in order to correct metabolic defects caused by enzyme deficiency. The formulation of ERTs involves the production, purification, and formulation of the enzyme into a stable and biologically active drug product, often using recombinant DNA technology. Non-systemic ERTs often involve the immobilization of the enzyme on a carrier, such as hydrogels, liposomes, or nanoparticles. ERT holds great promise for the treatment of a wide range of genetic disorders, and its success regarding lysosomal storage diseases, such as Fabry disease, Gaucher disease, and Pompe disease has paved the way for the development of similar therapies for other genetic disorders too.},
	year = {2023},
	eissn = {1587-3765},
	pages = {624-635},
	orcid-numbers = {Koplányi, Gábor/0000-0002-3791-1057; Balogh Weiser, Diána/0000-0002-9957-1203}
}

@article{MTMT:34096036,
	title = {Nanoformulation of lipase from Porcine pancreas by electrospinning as a novel alternative for enzyme-based per os therapies},
	url = {https://m2.mtmt.hu/api/publication/34096036},
	author = {Tóth, Gergő D. and Kállai-Szabó, Nikolett and Lengyel, Miléna and Süvegh, Károly and Ender, Ferenc and Katona, Gábor and Nochta-Kazsoki, Adrienn Katalin and Zelkó, Romána and Antal, István and Balogh, György Tibor and Balogh Weiser, Diána},
	doi = {10.1016/j.molliq.2023.122819},
	journal-iso = {J MOL LIQ},
	journal = {JOURNAL OF MOLECULAR LIQUIDS},
	volume = {389},
	unique-id = {34096036},
	issn = {0167-7322},
	year = {2023},
	eissn = {1873-3166},
	orcid-numbers = {Kállai-Szabó, Nikolett/0000-0002-8164-3993; Lengyel, Miléna/0000-0001-8865-054X; Süvegh, Károly/0000-0001-8147-0546; Katona, Gábor/0000-0003-1564-4813; Nochta-Kazsoki, Adrienn Katalin/0000-0002-0611-3124; Zelkó, Romána/0000-0002-5419-9137; Antal, István/0000-0002-5434-201X; Balogh, György Tibor/0000-0003-3347-1880; Balogh Weiser, Diána/0000-0002-9957-1203}
}

@article{MTMT:33740967,
	title = {Combined Nanofibrous Face Mask: Co-Formulation of Lipases and Antibiotic Agent by Electrospinning Technique},
	url = {https://m2.mtmt.hu/api/publication/33740967},
	author = {Balogh Weiser, Diána and Molnár, Alexandra and Tóth, Gergő Dániel and Koplányi, Gábor and Szemes, József and Decsi, Balázs and Katona, Gábor and Salamah, Maryana and Ender, Ferenc and Kovács, Anita and Berkó, Szilvia and Budai-Szűcs, Mária and Balogh, György Tibor},
	doi = {10.3390/pharmaceutics15041174},
	journal-iso = {PHARMACEUTICS},
	journal = {PHARMACEUTICS},
	volume = {15},
	unique-id = {33740967},
	issn = {1999-4923},
	abstract = {The application of enzyme-based therapies has received significant attention in modern drug development. Lipases are one of the most versatile enzymes that can be used as therapeutic agents in basic skin care and medical treatment related to excessive sebum production, acne, and inflammation. The traditional formulations available for skin treatment, such as creams, ointments or gels, are widely applied; however, their use is not always accompanied by good drug penetration properties, stability, or patient adherence. Nanoformulated drugs offer the possibility of combining enzymatic and small molecule formulations, making them a new and exciting alternative in this field. In this study polymeric nanofibrous matrices made of polyvinylpyrrolidone and polylactic acid were developed, entrapping lipases from Candida rugosa and Rizomucor miehei and antibiotic compound nadifloxacin. The effect of the type of polymers and lipases were investigated, and the nanofiber formation process was optimized to provide a promising alternative in topical treatment. Our experiments have shown that entrapment by electrospinning induced two orders of magnitude increase in the specific enzyme activity of lipases. Permeability investigations indicated that all lipase-loaded nanofibrous masks were capable of delivering nadifloxacin to the human epidermis, confirming the viability of electrospinning as a formulation method for topical skin medications.},
	keywords = {PROTEIN; Therapy; EFFICACY; ENZYMATIC RESOLUTION; LIPASE; acne vulgaris; Electrospinning; nanoformulation; Skin treatment; Immobilized lipase; nano mask},
	year = {2023},
	eissn = {1999-4923},
	orcid-numbers = {Balogh Weiser, Diána/0000-0002-9957-1203; Koplányi, Gábor/0000-0002-3791-1057; Katona, Gábor/0000-0003-1564-4813; Kovács, Anita/0000-0001-5593-1329; Berkó, Szilvia/0000-0002-3842-8876; Budai-Szűcs, Mária/0000-0001-5187-5702; Balogh, György Tibor/0000-0003-3347-1880}
}

@article{MTMT:33692858,
	title = {Natural Lipid Extracts as an Artificial Membrane for Drug Permeability Assay: In Vitro and In Silico Characterization},
	url = {https://m2.mtmt.hu/api/publication/33692858},
	author = {Vincze, Anna and Dékány, Gergely and Bicsak, Richárd and Formanek, András and Moreau, Yves and Koplányi, Gábor and Takács, Gergely and Katona, Gábor and Balogh Weiser, Diána and Arany, Ádám and Balogh, György Tibor},
	doi = {10.3390/pharmaceutics15030899},
	journal-iso = {PHARMACEUTICS},
	journal = {PHARMACEUTICS},
	volume = {15},
	unique-id = {33692858},
	issn = {1999-4923},
	abstract = {In vitro non-cellular permeability models such as the parallel artificial membrane permeability assay (PAMPA) are widely applied tools for early-phase drug candidate screening. In addition to the commonly used porcine brain polar lipid extract for modeling the blood–brain barrier’s permeability, the total and polar fractions of bovine heart and liver lipid extracts were investigated in the PAMPA model by measuring the permeability of 32 diverse drugs. The zeta potential of the lipid extracts and the net charge of their glycerophospholipid components were also determined. Physicochemical parameters of the 32 compounds were calculated using three independent forms of software (Marvin Sketch, RDKit, and ACD/Percepta). The relationship between the lipid-specific permeabilities and the physicochemical descriptors of the compounds was investigated using linear correlation, Spearman correlation, and PCA analysis. While the results showed only subtle differences between total and polar lipids, permeability through liver lipids highly differed from that of the heart or brain lipid-based models. Correlations between the in silico descriptors (e.g., number of amide bonds, heteroatoms, and aromatic heterocycles, accessible surface area, and H-bond acceptor–donor balance) of drug molecules and permeability values were also found, which provides support for understanding tissue-specific permeability.},
	year = {2023},
	eissn = {1999-4923},
	orcid-numbers = {Formanek, András/0000-0003-0734-1417; Koplányi, Gábor/0000-0002-3791-1057; Katona, Gábor/0000-0003-1564-4813; Balogh Weiser, Diána/0000-0002-9957-1203; Balogh, György Tibor/0000-0003-3347-1880}
}

@article{MTMT:33675955,
	title = {Effect of liposomal formulation of ascorbic acid on corneal permeability},
	url = {https://m2.mtmt.hu/api/publication/33675955},
	author = {Csorba, Anita and Katona, Gábor and Budai-Szűcs, Mária and Balogh Weiser, Diána and Fadda, Anna Maria and Caddeo, Carla and Takács, Ágnes Ildikó and Mátyus, Péter and Balogh, György Tibor and Nagy, Zoltán Zsolt},
	doi = {10.1038/s41598-023-29290-9},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {13},
	unique-id = {33675955},
	issn = {2045-2322},
	abstract = {Ascorbic acid (AA) has a pivotal role in corneal wound healing via stimulating the biosynthesis of highly organized extracellular matrix components, but its rapid degradation and low corneal permeability limits its therapeutic effects. In this paper, we present the pharmacokinetic properties of a liposomal-based formulation of AA in terms of corneal permeation. Chemical stability, shelf-life, and drug release rate of lyophilized liposome (AA-LLipo) formulation was determined in comparison to free-form of AA solution using high-performance liquid chromatography (HPLC) and rapid equilibrium dialysis. In vitro transcorneal permeability was studied using a parallel artificial membrane permeability assay (PAMPA). Ex vivo permeation was examined on AA-LLipo-treated porcine cornea by determining the AA content on the ocular surface, in the cornea as well as in the aqueous humor using HPLC, and by Raman-mapping visualizing the AA-distribution. Our results showed that the liposomal formulation improved the chemical stability of AA, while drug release was observed with the same kinetic efficiency as from the free-form of AA solution. Both corneal-PAMPA and porcine corneal permeability studies showed that AA-LLipo markedly improved the corneal absorption kinetics of AA, thus, increasing the AA content in the cornea and aqueous humor. AA-LLipo formulation could potentially increase the bioavailability of AA in corneal tissues.},
	year = {2023},
	eissn = {2045-2322},
	orcid-numbers = {Csorba, Anita/0000-0002-3256-9440; Katona, Gábor/0000-0003-1564-4813; Budai-Szűcs, Mária/0000-0001-5187-5702; Balogh Weiser, Diána/0000-0002-9957-1203; Takács, Ágnes Ildikó/0000-0002-3121-5106; Balogh, György Tibor/0000-0003-3347-1880; Nagy, Zoltán Zsolt/0000-0002-7330-0464}
}

@article{MTMT:33620537,
	title = {Magnetically agitated continuous-flow tube reactors with aspartate ammonia-lyase immobilized on magnetic nanoparticles},
	url = {https://m2.mtmt.hu/api/publication/33620537},
	author = {Imarah, Ali O. and Silva, Fausto M. W. G. and Bataa, Naran and Decsi, Balázs and Balogh Weiser, Diána and Poppe, László},
	doi = {10.1039/D2RE00507G},
	journal-iso = {REACT CHEM ENG},
	journal = {REACTION CHEMISTRY & ENGINEERING},
	volume = {8},
	unique-id = {33620537},
	issn = {2058-9883},
	abstract = {Two magnetically agitated continuous-flow tube reactors (AFRs)—applying external permanent magnets to move magnetic nanoparticles coated with a biocatalyst within a stream of the reaction medium—were developed and studied with aspartate ammonia-lyase (AAL) from Pseudomonas fluorescens immobilized onto epoxy-functionalized magnetic nanoparticles (MNPs) by covalent binding. The biotransformation of L-aspartate to fumarate by the AAL-MNPs (5 mg, D = 420 nm, 6 μg g−1 AAL) took place in the reaction tube (PTFE, ID 2.15 mm) of both AFRs in a space containing the AAL-MNPs agitated within the flow of the reaction medium (0.5 mM L-aspartate solution, 4.7–14 μL min−1, 25 °C) by two permanent ring magnets (N48 neodymium, 10 × 5 × 5 mm) positioned at a fixed distance in attraction mode. In the first version (AFRXM), the two magnets positioned at opposite sides of the reaction tube (distance: 20 mm) performed axial movement (amplitude: 8 mm, frequency: 40–140 mpm) along the Y-axis, being perpendicular to the X-axis of the tube. In the second version (AFRRM), the two magnets (distance: 10 mm from each other, 5 mm from the X-axis) performed rotation movement (frequency: 40–140 rpm) around the X-axis. Whereas in the AFRXM the AAL-MNPs formed a cloud moving back and forth, they created a ring-shaped cloud rotating within the tube in the AFRRM. The efficient internal mixing in the AFRRM at the best frequency (80 rpm) resulted in the highest apparent specific activity (Ub = 354–469 U g−1, at residence times of 2.5–7.5 min) of the AAL-MNPs in the reactors studied. In the other continuous-flow systems, significantly lower Ub values were achieved (135–290 U g−1 at 120 mpm in AFRXM; or 142–273 U g−1 and 64–129 U g−1 in tubular reactors anchoring MNPs in static mode with double or single magnets, respectively), whereas more than a magnitude of order lower values could be realised in the batch mode reactors (11.4–14.9 U g−1 with rotational magnetic agitation at 120 rpm; 5.0–5.8 U g−1 with axial magnetic agitation at 160 mpm; or 4.6–5.2 U g−1 in an orbital shaker at 600 rpm) at comparable reaction times (2.5–7.5 min).},
	year = {2023},
	eissn = {2058-9883},
	pages = {1250-1259},
	orcid-numbers = {Bataa, Naran/0000-0001-8827-250X; Balogh Weiser, Diána/0000-0002-9957-1203; Poppe, László/0000-0002-8358-1378}
}

@article{MTMT:33574611,
	title = {Novel Approach for the Isolation and Immobilization of a Recombinant Transaminase. Applying an Advanced Nanocomposite System},
	url = {https://m2.mtmt.hu/api/publication/33574611},
	author = {Koplányi, Gábor and Bell, Evelin and Molnár, Zsófia Klára and Katona, Gábor and Neumann, Péter Lajos and Ender, Ferenc and Balogh, György Tibor and Žnidaršič-Plazl, Polona and Poppe, László and Balogh Weiser, Diána},
	doi = {10.1002/cbic.202200713},
	journal-iso = {CHEMBIOCHEM},
	journal = {CHEMBIOCHEM},
	volume = {24},
	unique-id = {33574611},
	issn = {1439-4227},
	year = {2023},
	eissn = {1439-7633},
	orcid-numbers = {Koplányi, Gábor/0000-0002-3791-1057; Katona, Gábor/0000-0003-1564-4813; Neumann, Péter Lajos/0000-0002-2881-5733; Ender, Ferenc/0000-0003-3800-5707; Balogh, György Tibor/0000-0003-3347-1880; Poppe, László/0000-0002-8358-1378; Balogh Weiser, Diána/0000-0002-9957-1203}
}

@article{MTMT:33363235,
	title = {Effect of Binding Linkers on the Efficiency and Metabolite Profile of Biomimetic Reactions Catalyzed by Immobilized Metalloporphyrin},
	url = {https://m2.mtmt.hu/api/publication/33363235},
	author = {Balogh, György Tibor and Decsi, Balázs and Krammer, Réka and Kenéz, Balázs and Ender, Ferenc and Hergert, Tamás and Balogh Weiser, Diána},
	doi = {10.3390/metabo12121269},
	journal-iso = {METABOLITES},
	journal = {METABOLITES},
	volume = {12},
	unique-id = {33363235},
	issn = {2218-1989},
	abstract = {The investigation of liver-related metabolic stability of a drug candidate is a widely used key strategy in early-stage drug discovery. Metalloporphyrin-based biomimetic catalysts are good and well-described models of the function of CyP450 in hepatocytes. In this research, the immobilization of an iron porphyrin was performed on nanoporous silica particles via ionic interactions. The effect of the metalloporphyrin binding linkers was investigated on the catalytic efficiency and the metabolic profile of chloroquine as a model drug. The length of the amino-substituted linkers affects the chloroquine conversion as well as the ratio of human major and minor metabolites. While testing the immobilized catalysts in the continuous-flow reactor, results showed that the presented biomimetic system could be a promising alternative for the early-stage investigation of drug metabolites regarding analytical or synthetic goals as well.},
	year = {2022},
	eissn = {2218-1989},
	pages = {1269-1282},
	orcid-numbers = {Balogh, György Tibor/0000-0003-3347-1880; Krammer, Réka/0000-0001-9814-6347; Balogh Weiser, Diána/0000-0002-9957-1203}
}

@article{MTMT:33229099,
	title = {Novel biomimetic nanocomposite for investigation of drug metabolism},
	url = {https://m2.mtmt.hu/api/publication/33229099},
	author = {Balogh Weiser, Diána and Poppe, László and Kenéz, Balázs and Decsi, Balázs and Koplányi, Gábor and Katona, Gábor and Gyarmati, Benjámin Sándor and Ender, Ferenc and Balogh, György Tibor},
	doi = {10.1016/j.molliq.2022.120781},
	journal-iso = {J MOL LIQ},
	journal = {JOURNAL OF MOLECULAR LIQUIDS},
	volume = {368},
	unique-id = {33229099},
	issn = {0167-7322},
	abstract = {In vitro mimicking of hepatic drug metabolism is a key issue in early-stage drug discovery. Synthetic metalloporphyrins show structural similarity with the heme type prosthetic group of cytochrome P450 as primary hepatic enzyme in oxidative drug biotransformation. Therefore, they can catalyze these oxidations. Concerning economical aspects and the poor stability of metalloporphyrin, their immobilization onto or into solid carriers can be promising solution. This study presents a novel immobilized metalloporphyrin nanocomposite system and its potential use as biomimetic catalysts. The developed two-step immobilization procedure consists of two main steps. First, the ionic binding of meso-tetra (parasulphonatophenyl) iron porphyrin onto functionalized magnetic nanoparticles is established, followed by embedding the nanoparticles into polylactic acid nanofibers by electrospinning technique. Due to the synergistic morphological and chemo-structural advantages of binding onto nanoparticles and embedding in polymeric matrices the biomimetic efficiency of metalloporphyrin can be remarkably enhanced, while substrate conversion value was tenfold larger than which could be achieved with classic human liver microsomal system.},
	keywords = {IMMOBILIZATION; COMPOSITES; OXIDATION; NANOFIBERS; Biotransformation; Chemistry, Physical; FABRICATION; magnetic nanoparticles; Metalloporphyrins; CATALYTIC-ACTIVITY},
	year = {2022},
	eissn = {1873-3166},
	orcid-numbers = {Balogh Weiser, Diána/0000-0002-9957-1203; Poppe, László/0000-0002-8358-1378; Koplányi, Gábor/0000-0002-3791-1057; Katona, Gábor/0000-0003-1564-4813; Ender, Ferenc/0000-0003-3800-5707; Balogh, György Tibor/0000-0003-3347-1880}
}