TY - JOUR AU - Olajos, Gábor AU - Bartus, Éva AU - Schuster, Ildikó AU - Lautner, Gergely AU - Gyurcsányi, Ervin Róbert AU - Szögi, Titanilla AU - Fülöp, Lívia AU - Martinek, Tamás TI - Multivalent foldamer-based affinity assay for selective recognition of Aβ oligomers JF - ANALYTICA CHIMICA ACTA J2 - ANAL CHIM ACTA VL - 960 PY - 2017 SP - 131 EP - 137 PG - 7 SN - 0003-2670 DO - 10.1016/j.aca.2017.01.013 UR - https://m2.mtmt.hu/api/publication/3193785 ID - 3193785 N1 - Megjegyzés-26494651 N1 Funding details: TÁMOP-4.2.4.A/2-11/1-2012-0001, ESF, European Social Fund N1 Funding text: This work was supported by the Hungarian Academy of Sciences, Lendület programs (LP-2011-009 and LP2013-63), Gedeon Richter Plc. (TP7-017), the Hungarian Research Foundation (OTKA K112442) and Gedeon Richter's Talentum Foundation (Ph.D. Scholarship to É.B.). This research received financing also from the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TÁMOP-4.2.4.A/2-11/1-2012-0001 ‘National Excellence Program’. This work was supported by the Hungarian Brain Research Program - Grant No. KTIA_13_NAP-A-III/7. Funding Agency and Grant Number: Hungarian Academy of Sciences, Lendulet program [LP-2011-009, LP2013-63]; Gedeon Richter Plc. [TP7-017]; Hungarian Research Foundation [OTKA K112442]; Gedeon Richter's Talentum Foundation; European Union; State of Hungary; European Social Fund 'National Excellence Program' [TAMOP-4.2.4.A/2-11/1-2012-0001]; Hungarian Brain Research Program [KTIA_13_-NAP-A-III/7]\n Funding text: This work was supported by the Hungarian Academy of Sciences, Lendulet programs (LP-2011-009 and LP2013-63), Gedeon Richter Plc. (TP7-017), the Hungarian Research Foundation (OTKA K112442) and Gedeon Richter's Talentum Foundation (Ph.D. Scholarship to E.B.). This research received financing also from the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TAMOP-4.2.4.A/2-11/1-2012-0001 'National Excellence Program'. This work was supported by the Hungarian Brain Research Program - Grant No. KTIA_13_-NAP-A-III/7.\n Funding Agency and Grant Number: Hungarian Academy of Sciences, Lendulet program [LP-2011-009, LP2013-63]; Gedeon Richter Plc. [TP7-017]; Hungarian Research Foundation [OTKA K112442]; Gedeon Richter's Talentum Foundation; European Union; State of Hungary; European Social Fund 'National Excellence Program' [TAMOP-4.2.4.A/2-11/1-2012-0001]; Hungarian Brain Research Program [KTIA_13_-NAP-A-III/7] Funding text: This work was supported by the Hungarian Academy of Sciences, Lendulet programs (LP-2011-009 and LP2013-63), Gedeon Richter Plc. (TP7-017), the Hungarian Research Foundation (OTKA K112442) and Gedeon Richter's Talentum Foundation (Ph.D. Scholarship to E.B.). This research received financing also from the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TAMOP-4.2.4.A/2-11/1-2012-0001 'National Excellence Program'. This work was supported by the Hungarian Brain Research Program - Grant No. KTIA_13_-NAP-A-III/7. Institute of Pharmaceutical Analysis, University of Szeged, Somogyi u. 4, Szeged, 6720, Hungary Department of Medical Chemistry, University of Szeged, Dóm tér 8, Szeged, 6720, Hungary MTA-BME Lendület Chemical Nanosensors Research Group, Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, Budapest, 1111, Hungary Cited By :2 Export Date: 24 September 2019 CODEN: ACACA Correspondence Address: Fülöp, L.; Department of Medical Chemistry, University of Szeged, Dóm tér 8, Hungary; email: fulop.livia@med.u-szeged.hu Chemicals/CAS: amyloid beta protein, 109770-29-8; Amyloid beta-Peptides; Protein Aggregates Funding details: Richter Gedeon Talentum Alapítvány Funding details: European Social Fund, ESF, A/2-11/1-2012-0001 Funding details: Magyar Tudományos Akadémia, MTA, LP2013-63, LP-2011-009 Funding details: Gedeon Richter, TP7-017 Funding details: K112442 Funding details: KTIA_13_NAP-A-III/7 Funding text 1: This work was supported by the Hungarian Academy of Sciences, Lend?let programs (LP-2011-009 and LP2013-63), Gedeon Richter Plc. (TP7-017), the Hungarian Research Foundation (OTKA K112442) and Gedeon Richter's Talentum Foundation (Ph.D. Scholarship to ?.B.). This research received financing also from the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of T?MOP-4.2.4.A/2-11/1-2012-0001 ?National Excellence Program?. This work was supported by the Hungarian Brain Research Program - Grant No. KTIA_13_NAP-A-III/7. CAplus AN 2017:198655; MEDLINE PMID: 28193356 (Journal; Article); AB - Abstract Mimicking the molecular recognition functionality of antibodies is a great challenge. Foldamers are attractive candidates because of their relatively small size and designable interaction surface. This paper describes a sandwich type enzyme-linked immunoassay with a tetravalent β-peptide foldamer helix array as capture element and enzyme labeled tracer antibodies. The assay was found to be selective to β-amyloid oligomeric species with surface features transiently present in ongoing aggregation. In optimized conditions, with special emphasis on the foldamer immobilization, a detection limit of 5 pM was achieved with a linear range of 10–500 pM. These results suggest that protein mimetic foldamers can be useful tools in biosensors and affinity assays. LA - English DB - MTMT ER - TY - JOUR AU - Lautner, Gergely AU - Plesz, M AU - Jágerszki, Gyula AU - Fürjes, Péter AU - Gyurcsányi, Ervin Róbert TI - Nanoparticle displacement assay with electrochemical nanopore-based sensors JF - ELECTROCHEMISTRY COMMUNICATIONS J2 - ELECTROCHEM COMMUN VL - 71 PY - 2016 SP - 13 EP - 17 PG - 5 SN - 1388-2481 DO - 10.1016/j.elecom.2016.07.012 UR - https://m2.mtmt.hu/api/publication/3113127 ID - 3113127 AB - The proof of concept of a nanoparticle displacement assay that enables the use of large diameter nanopores for the detection of targets of smaller molecular dimensions is presented. We hypothesized that an inherent signal amplification should arise from the selective displacement of nanoparticles preloaded in a nanopore by a much smaller molecular target. The method is demonstrated using peptide nucleic acid (PNA)-functionalized gold nanopore arrays in which short DNA-modified gold nanoparticles are anchored by weak interaction. Complementary microRNAs are detected via the resistance change caused by competitive displacement of nanoparticles from the PNA-functionalized nanopores. © 2016 Elsevier B.V. LA - English DB - MTMT ER - TY - JOUR AU - Lautner, Gergely AU - Meyerhoff, Mark E AU - Schwendeman, Steven P TI - Biodegradable poly(lactic-co-glycolic acid) microspheres loaded with S-nitroso-N-acetyl-D-penicillamine for controlled nitric oxide delivery JF - JOURNAL OF CONTROLLED RELEASE J2 - J CONTROL RELEASE VL - 225 PY - 2016 SP - 133 EP - 139 PG - 7 SN - 0168-3659 DO - 10.1016/j.jconrel.2015.12.056 UR - https://m2.mtmt.hu/api/publication/3028280 ID - 3028280 AB - Abstract Nitric oxide (NO) is a fascinating and important endogenous free-radical gas with potent antimicrobial, vasodilating, smooth muscle relaxant, and growth factor stimulating effects. However, its wider biomedical applicability is hindered by its cumbersome administration, since NO is unstable especially in biological environments. In this work, to ultimately develop site-specific controlled release vehicles for NO, the NO donor S-nitroso-N-acetyl-D-penicillamine (SNAP) was encapsulated within poly(lactic-co-glycolic acid) 50:50 (PLGA) microspheres by using a solid-in-oil-in-water emulsion solvent evaporation method. The highest payload was 0.56(± 0.01) μmol SNAP/mg microspheres. The in vitro release kinetics of the donor were controlled by the bioerosion of the PLGA microspheres. By using an uncapped PLGA (Mw = 24,000–38,000) SNAP was slowly released for over 10 days, whereas by using the ester capped PLGA (Mw = 38,000–54,000) the release lasted for over 4 weeks. The presence of copper ions and/or ascorbate in solution was necessary to efficiently decompose the released NO donor and obtain sustained NO release. It was also demonstrated that light can be used to induce rapid NO release from the microspheres over several hours. SNAP exhibited excellent storage stability when encapsulated in the PLGA microspheres. These new microsphere formulations may be useful for site-specific administration and treatment of pathologies associated with dysfunction in endogenous NO production, e.g. treatment of diabetic wounds, or in diseases involving other biological functions of NO including vasodilation, antimicrobial, anticancer, and neurotransmission. LA - English DB - MTMT ER - TY - JOUR AU - Simon, László Ferenc AU - Lautner, Gergely AU - Gyurcsányi, Ervin Róbert TI - Reliable microspotting methodology for peptide-nucleic acid layers with high hybridization efficiency on gold SPR imaging chips JF - ANALYTICAL METHODS: ADVANCING METHODS AND APPLICATIONS J2 - ANAL METHOD VL - 7 PY - 2015 IS - 15 SP - 6077 EP - 6082 PG - 6 SN - 1759-9660 DO - 10.1039/c5ay01239b UR - https://m2.mtmt.hu/api/publication/2926219 ID - 2926219 N1 - MTA-BME Lendület Chemical Nanosensors Research Group, Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szent Gellért tér 4, Budapest, H-1111, Hungary Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szent Gellért tér 4, Budapest, H-1111, Hungary Cited By :10 Export Date: 25 March 2021 Correspondence Address: Gyurcsányi, R.E.; MTA-BME Lendület Chemical Nanosensors Research Group, Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szent Gellért tér 4, Hungary AB - One-step direct immobilization of peptide-nucleic acid (PNA) probes onto gold surfaces through Au-S chemistry is critical in terms of generating self-assembled monolayers with high hybridization efficiency. We found that this problem is more severe if the immobilization is performed by contact microspotting to generate PNA arrays. Therefore, here we propose a novel microspotting-based immobilization method to generate PNA arrays with high hybridization efficiency on bare gold surface plasmon resonance imaging (SPRi) chips. The essence of the approach is to spot thiol labelled PNA strands prehybridized with a short complementary DNA strand instead of conventionally used single stranded PNA (ssPNA) probes. After immobilization the complementary DNA strands could be easily removed to activate the surface confined PNA probes. The incubation time and the type of spotting needle also have a marked influence on the hybridization efficiency of the PNA layers. However, we show that if all other conditions remain the same, PNA layers from prehybridized PNA probes exhibit superior hybridization efficiency than those from ssPNA probes. This journal is © The Royal Society of Chemistry 2015. LA - English DB - MTMT ER - TY - JOUR AU - Bosserdt, M AU - Bognár, Júlia AU - Lautner, Gergely AU - Witt, J AU - Köhler, K AU - Gajovich-Eichelmann, N AU - Yarman, A AU - Wittstock, G AU - Scheller, FW AU - Gyurcsányi, Ervin Róbert TI - Microelectrospotting as a new method for electrosynthesis of surface-imprinted polymer microarrays for protein recognition JF - BIOSENSORS & BIOELECTRONICS J2 - BIOSENS BIOELECTRON VL - 73 PY - 2015 SP - 123 EP - 129 PG - 7 SN - 0956-5663 DO - 10.1016/j.bios.2015.05.049 UR - https://m2.mtmt.hu/api/publication/2906358 ID - 2906358 N1 - Fraunhofer Institute IZI-BB, Potsdam, D-14476, Germany MTA-BME Research Group of Technical Analytical Chemistry, Szt. Gellért tér 4, Budapest, H-1111, Hungary Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szent Gellért tér 4, Budapest, H-1111, Hungary Carl von Ossietzky University of Oldenburg, Department of Chemistry, Oldenburg, D-26111, Germany MTA-BME Lendület Chemical Nanosensors Research Group, Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szent Gellért tér 4, Budapest, H-1111, Hungary Cited By :31 Export Date: 25 March 2021 CODEN: BBIOE Correspondence Address: Gyurcsányi, R.E.; MTA-BME Lendület Chemical Nanosensors Research Group, Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, Hungary Chemicals/CAS: ferritin, 9007-73-2; scopoletin, 92-61-5; gold, 7440-57-5; Ferritins; Gold; Polymers; Scopoletin; Serum Albumin, Bovine Funding details: 1074-49.10/2009 Funding details: 93719903 Funding details: Deutsche Forschungsgemeinschaft, DFG, EXC 314 Funding details: Bundesministerium für Bildung und Forschung, BMBF Funding details: Hungarian Scientific Research Fund, OTKA, K104724 Funding details: Magyar Tudományos Akadémia, MTA, LP2013-63/2013 Funding text 1: The financial support of the Lendület program of the Hungarian Academy of Sciences ( LP2013-63/2013 ), ERA-Chemistry, and the Hungarian Scientific Fund ( K104724 ) is gratefully acknowledged. F.W.S. gratefully acknowledges the financial support of Deutsche Forschungsgemeinschaft (DFG) within the framework of the German Excellence Initiative ( EXC 314 ), the BMBF in TERA-Sens ( 93719903 ) J.W. thanks the German Israeli Foundation for research funding (Grant no. 1074-49.10/2009 ). Fraunhofer Institute IZI-BB, Potsdam, D-14476, Germany MTA-BME Research Group of Technical Analytical Chemistry, Szt. Gellért tér 4, Budapest, H-1111, Hungary Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szent Gellért tér 4, Budapest, H-1111, Hungary Carl von Ossietzky University of Oldenburg, Department of Chemistry, Oldenburg, D-26111, Germany MTA-BME Lendület Chemical Nanosensors Research Group, Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szent Gellért tér 4, Budapest, H-1111, Hungary Cited By :31 Export Date: 27 March 2021 CODEN: BBIOE Correspondence Address: Gyurcsányi, R.E.; MTA-BME Lendület Chemical Nanosensors Research Group, Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, Hungary Chemicals/CAS: ferritin, 9007-73-2; scopoletin, 92-61-5; gold, 7440-57-5; Ferritins; Gold; Polymers; Scopoletin; Serum Albumin, Bovine Funding details: 1074-49.10/2009 Funding details: 93719903 Funding details: Deutsche Forschungsgemeinschaft, DFG, EXC 314 Funding details: Bundesministerium für Bildung und Forschung, BMBF Funding details: Hungarian Scientific Research Fund, OTKA, K104724 Funding details: Magyar Tudományos Akadémia, MTA, LP2013-63/2013 Funding text 1: The financial support of the Lendület program of the Hungarian Academy of Sciences ( LP2013-63/2013 ), ERA-Chemistry, and the Hungarian Scientific Fund ( K104724 ) is gratefully acknowledged. F.W.S. gratefully acknowledges the financial support of Deutsche Forschungsgemeinschaft (DFG) within the framework of the German Excellence Initiative ( EXC 314 ), the BMBF in TERA-Sens ( 93719903 ) J.W. thanks the German Israeli Foundation for research funding (Grant no. 1074-49.10/2009 ). LA - English DB - MTMT ER - TY - JOUR AU - Lautner, Gergely AU - Gyurcsányi, Ervin Róbert TI - Szelektív szintetikus receptorok fejlesztése és alkalmazása fehérjék meghatározására JF - MAGYAR KÉMIAI FOLYÓIRAT - KÉMIAI KÖZLEMÉNYEK (1997-) J2 - MAGY KÉM FOLY KÉM KÖZL VL - 120 PY - 2014 IS - 1 SP - 32 EP - 37 PG - 6 SN - 1418-9933 UR - https://m2.mtmt.hu/api/publication/2790830 ID - 2790830 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Lautner, Gergely AU - Gyurcsányi, Ervin Róbert TI - Electrochemical Detection of miRNAs JF - ELECTROANALYSIS J2 - ELECTROANAL VL - 26 PY - 2014 IS - 6 SP - 1224 EP - 1235 PG - 12 SN - 1040-0397 DO - 10.1002/elan.201400055 UR - https://m2.mtmt.hu/api/publication/2706894 ID - 2706894 N1 - Funding Agency and Grant Number: ENIAC (CAJAL4EU); Momentum (Lendulet) program of the Hungarian Academy of Sciences [LP2013-63/2013]; New Szechenyi Plan [TAMOP-4.2.1./B-09/1/KMR-2010-0002] Funding text: The financial support of ENIAC (CAJAL4EU), the Momentum (Lendulet) program of the Hungarian Academy of Sciences (LP2013-63/2013) and of New Szechenyi Plan (TAMOP-4.2.1./B-09/1/KMR-2010-0002). Cited By :30 Export Date: 25 March 2021 CODEN: ELANE Cited By :30 Export Date: 27 March 2021 CODEN: ELANE LA - English DB - MTMT ER - TY - JOUR AU - Szeitner, Zsuzsanna AU - Lautner, Gergely AU - Nagy, Szilvia Krisztina AU - Gyurcsányi, Ervin Róbert AU - Mészáros, Tamás TI - A rational approach for generating cardiac troponin I selective Spiegelmers JF - CHEMICAL COMMUNICATIONS J2 - CHEM COMMUN VL - 50 PY - 2014 IS - 51 SP - 6801 EP - 6804 PG - 4 SN - 1359-7345 DO - 10.1039/C4CC00447G UR - https://m2.mtmt.hu/api/publication/2590382 ID - 2590382 AB - We report the first protein selective Spiegelmers of diagnostic relevance by rational identification of a target epitope and reverse screening of Spiegelmer candidates following the selection procedure. Application of the presented approach resulted in isolation of cardiac troponin I selective Spiegelmers with low nanomolar dissociation constant and functionality in serum. LA - English DB - MTMT ER - TY - THES AU - Lautner, Gergely TI - Szelektiv szintetikus receptorok fejlesztése és alkalmazása fehérjék meghatározására PB - Budapesti Műszaki és Gazdaságtudományi Egyetem PY - 2013 UR - https://m2.mtmt.hu/api/publication/2944046 ID - 2944046 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Lautner, Gergely AU - Balogh, Zsófia AU - Gyurkovics, A AU - Gyurcsányi, Ervin Róbert AU - Mészáros, Tamás TI - Homogeneous assay for evaluation of aptamer-protein interaction JF - ANALYST J2 - ANALYST VL - 137 PY - 2012 IS - 17 SP - 3929 EP - 3931 PG - 3 SN - 0003-2654 DO - 10.1039/c2an35419e UR - https://m2.mtmt.hu/api/publication/2039581 ID - 2039581 AB - We introduce Amplified Luminescent Proximity Homogenous Assay (ALPHA) to assess the K D value of aptamer-protein complexes as demonstrated through the study of apple stem pitting virus coat protein-specific aptamers. This method can be used as a simple, cost-effective method for screening aptamer-target protein interactions during aptamer selection. © 2012 The Royal Society of Chemistry. LA - English DB - MTMT ER -