TY - JOUR AU - Fajka-Boja, Roberta AU - Marton, Annamária AU - Tóth, Anna AU - Blazsó, Péter AU - Tubak, Vilmos AU - Bálint, Balázs AU - Nagy, István AU - Hegedűs, Zoltán AU - Vizler, Csaba AU - Katona, Róbert László TI - Adipose stem cells may promote cancer progression JF - RESEARCH OUTREACH J2 - RO VL - 2019 PY - 2019 SP - 6 EP - 9 PG - 4 SN - 2517-701X DO - 10.32907/RO-106-110113 UR - https://m2.mtmt.hu/api/publication/30693937 ID - 30693937 LA - English DB - MTMT ER - TY - JOUR AU - Fajka-Boja, Roberta AU - Marton, Annamária AU - Tóth, Anna AU - Blazsó, Péter AU - Tubak, Vilmos AU - Bálint, Balázs AU - Nagy, István AU - Hegedűs, Zoltán AU - Vizler, Csaba AU - Katona, Róbert László TI - Increased insulin-like growth factor 1 production by polyploid adipose stem cells promotes growth of breast cancer cells JF - BMC CANCER J2 - BMC CANCER VL - 18 PY - 2018 PG - 12 SN - 1471-2407 DO - 10.1186/s12885-018-4781-z UR - https://m2.mtmt.hu/api/publication/3411662 ID - 3411662 LA - English DB - MTMT ER - TY - THES AU - Tóth, Anna TI - Egy emlős mesterséges kromoszóma több génnel történő feltöltésének új módszere PB - Szegedi Tudományegyetem (SZTE) PY - 2014 SP - 77 DO - 10.14232/phd.2073 UR - https://m2.mtmt.hu/api/publication/2781999 ID - 2781999 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Tóth, Anna AU - Fodor, Katalin AU - Blazsó, Péter AU - Cserpán, Imre AU - Praznovszky, Tünde AU - Tubak, Vilmos AU - Udvardy, Andor AU - Hadlaczky, Gyula AU - Katona, Róbert László TI - Generation of induced pluripotent stem cells by using a mammalian artificial chromosome expression system. JF - ACTA BIOLOGICA HUNGARICA (1983-2018) J2 - ACTA BIOL HUNG VL - 65 PY - 2014 IS - 3 SP - 331 EP - 345 PG - 15 SN - 0236-5383 DO - 10.1556/ABiol.65.2014.3.9 UR - https://m2.mtmt.hu/api/publication/2731564 ID - 2731564 AB - Direct reprogramming of mouse fibroblasts into induced pluripotent stem cells (iPS) was achieved recently by overexpression of four transcription factors encoded by retroviral vectors. Most of the virus vectors, however, may cause insertional mutagenesis in the host genome and may also induce tumor formation. Therefore, it is very important to discover novel and safer, non-viral reprogramming methods. Here we describe the reprogramming of somatic cells into iPS cells by a novel protein-based technique. Engineered Oct4, Sox2 and Klf4 transcription factors carrying an N-terminal Flag-tag and a C-terminal polyarginine tail were synthesized by a recently described mammalian artificial chromosome expression system (ACEs). This system is suitable for the high-level production of recombinant proteins in mammalian tissue culture cells. Recombinant proteins produced in this system contain all the post-translational modifications essential for the stability and the authentic function of the proteins. The engineered Oct4, Sox2 and Klf4 proteins efficiently induced the reprogramming of mouse embryonic fibroblasts by means of protein transduction. This novel method allows for the generation of iPS cells, which may be suitable for therapeutic applications in the future. LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Anna AU - Fodor, Katalin AU - Praznovszky, Tünde AU - Tubak, Vilmos AU - Udvardy, Andor AU - Hadlaczky, Gyula AU - Katona, Róbert László TI - Novel Method to Load Multiple Genes onto a Mammalian Artificial Chromosome. JF - PLOS ONE J2 - PLOS ONE VL - 9 PY - 2014 IS - 1 PG - 9 SN - 1932-6203 DO - 10.1371/journal.pone.0085565 UR - https://m2.mtmt.hu/api/publication/2508201 ID - 2508201 AB - Mammalian artificial chromosomes are natural chromosome-based vectors that may carry a vast amount of genetic material in terms of both size and number. They are reasonably stable and segregate well in both mitosis and meiosis. A platform artificial chromosome expression system (ACEs) was earlier described with multiple loading sites for a modified lambda-integrase enzyme. It has been shown that this ACEs is suitable for high-level industrial protein production and the treatment of a mouse model for a devastating human disorder, Krabbe's disease. ACEs-treated mutant mice carrying a therapeutic gene lived more than four times longer than untreated counterparts. This novel gene therapy method is called combined mammalian artificial chromosome-stem cell therapy. At present, this method suffers from the limitation that a new selection marker gene should be present for each therapeutic gene loaded onto the ACEs. Complex diseases require the cooperative action of several genes for treatment, but only a limited number of selection marker genes are available and there is also a risk of serious side-effects caused by the unwanted expression of these marker genes in mammalian cells, organs and organisms. We describe here a novel method to load multiple genes onto the ACEs by using only two selectable marker genes. These markers may be removed from the ACEs before therapeutic application. This novel technology could revolutionize gene therapeutic applications targeting the treatment of complex disorders and cancers. It could also speed up cell therapy by allowing researchers to engineer a chromosome with a predetermined set of genetic factors to differentiate adult stem cells, embryonic stem cells and induced pluripotent stem (iPS) cells into cell types of therapeutic value. It is also a suitable tool for the investigation of complex biochemical pathways in basic science by producing an ACEs with several genes from a signal transduction pathway of interest. LA - English DB - MTMT ER -