@article{MTMT:30693937, title = {Adipose stem cells may promote cancer progression}, url = {https://m2.mtmt.hu/api/publication/30693937}, author = {Fajka-Boja, Roberta and Marton, Annamária and Tóth, Anna and Blazsó, Péter and Tubak, Vilmos and Bálint, Balázs and Nagy, István and Hegedűs, Zoltán and Vizler, Csaba and Katona, Róbert László}, doi = {10.32907/RO-106-110113}, journal-iso = {RO}, journal = {RESEARCH OUTREACH}, volume = {2019}, unique-id = {30693937}, issn = {2517-701X}, year = {2019}, eissn = {2517-7028}, pages = {6-9}, orcid-numbers = {Blazsó, Péter/0000-0003-4404-8068; Tubak, Vilmos/0000-0002-6141-3920} } @article{MTMT:3411662, title = {Increased insulin-like growth factor 1 production by polyploid adipose stem cells promotes growth of breast cancer cells}, url = {https://m2.mtmt.hu/api/publication/3411662}, author = {Fajka-Boja, Roberta and Marton, Annamária and Tóth, Anna and Blazsó, Péter and Tubak, Vilmos and Bálint, Balázs and Nagy, István and Hegedűs, Zoltán and Vizler, Csaba and Katona, Róbert László}, doi = {10.1186/s12885-018-4781-z}, journal-iso = {BMC CANCER}, journal = {BMC CANCER}, volume = {18}, unique-id = {3411662}, issn = {1471-2407}, year = {2018}, eissn = {1471-2407}, orcid-numbers = {Fajka-Boja, Roberta/0000-0001-5331-8280; Blazsó, Péter/0000-0003-4404-8068; Tubak, Vilmos/0000-0002-6141-3920} } @mastersthesis{MTMT:2781999, title = {Egy emlős mesterséges kromoszóma több génnel történő feltöltésének új módszere}, url = {https://m2.mtmt.hu/api/publication/2781999}, author = {Tóth, Anna}, doi = {10.14232/phd.2073}, publisher = {SZTE}, unique-id = {2781999}, year = {2014} } @article{MTMT:2731564, title = {Generation of induced pluripotent stem cells by using a mammalian artificial chromosome expression system.}, url = {https://m2.mtmt.hu/api/publication/2731564}, author = {Tóth, Anna and Fodor, Katalin and Blazsó, Péter and Cserpán, Imre and Praznovszky, Tünde and Tubak, Vilmos and Udvardy, Andor and Hadlaczky, Gyula and Katona, Róbert László}, doi = {10.1556/ABiol.65.2014.3.9}, journal-iso = {ACTA BIOL HUNG}, journal = {ACTA BIOLOGICA HUNGARICA (1983-2018)}, volume = {65}, unique-id = {2731564}, issn = {0236-5383}, abstract = {Direct reprogramming of mouse fibroblasts into induced pluripotent stem cells (iPS) was achieved recently by overexpression of four transcription factors encoded by retroviral vectors. Most of the virus vectors, however, may cause insertional mutagenesis in the host genome and may also induce tumor formation. Therefore, it is very important to discover novel and safer, non-viral reprogramming methods. Here we describe the reprogramming of somatic cells into iPS cells by a novel protein-based technique. Engineered Oct4, Sox2 and Klf4 transcription factors carrying an N-terminal Flag-tag and a C-terminal polyarginine tail were synthesized by a recently described mammalian artificial chromosome expression system (ACEs). This system is suitable for the high-level production of recombinant proteins in mammalian tissue culture cells. Recombinant proteins produced in this system contain all the post-translational modifications essential for the stability and the authentic function of the proteins. The engineered Oct4, Sox2 and Klf4 proteins efficiently induced the reprogramming of mouse embryonic fibroblasts by means of protein transduction. This novel method allows for the generation of iPS cells, which may be suitable for therapeutic applications in the future.}, year = {2014}, eissn = {1588-256X}, pages = {331-345}, orcid-numbers = {Blazsó, Péter/0000-0003-4404-8068; Tubak, Vilmos/0000-0002-6141-3920} } @article{MTMT:2508201, title = {Novel Method to Load Multiple Genes onto a Mammalian Artificial Chromosome.}, url = {https://m2.mtmt.hu/api/publication/2508201}, author = {Tóth, Anna and Fodor, Katalin and Praznovszky, Tünde and Tubak, Vilmos and Udvardy, Andor and Hadlaczky, Gyula and Katona, Róbert László}, doi = {10.1371/journal.pone.0085565}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {9}, unique-id = {2508201}, issn = {1932-6203}, abstract = {Mammalian artificial chromosomes are natural chromosome-based vectors that may carry a vast amount of genetic material in terms of both size and number. They are reasonably stable and segregate well in both mitosis and meiosis. A platform artificial chromosome expression system (ACEs) was earlier described with multiple loading sites for a modified lambda-integrase enzyme. It has been shown that this ACEs is suitable for high-level industrial protein production and the treatment of a mouse model for a devastating human disorder, Krabbe's disease. ACEs-treated mutant mice carrying a therapeutic gene lived more than four times longer than untreated counterparts. This novel gene therapy method is called combined mammalian artificial chromosome-stem cell therapy. At present, this method suffers from the limitation that a new selection marker gene should be present for each therapeutic gene loaded onto the ACEs. Complex diseases require the cooperative action of several genes for treatment, but only a limited number of selection marker genes are available and there is also a risk of serious side-effects caused by the unwanted expression of these marker genes in mammalian cells, organs and organisms. We describe here a novel method to load multiple genes onto the ACEs by using only two selectable marker genes. These markers may be removed from the ACEs before therapeutic application. This novel technology could revolutionize gene therapeutic applications targeting the treatment of complex disorders and cancers. It could also speed up cell therapy by allowing researchers to engineer a chromosome with a predetermined set of genetic factors to differentiate adult stem cells, embryonic stem cells and induced pluripotent stem (iPS) cells into cell types of therapeutic value. It is also a suitable tool for the investigation of complex biochemical pathways in basic science by producing an ACEs with several genes from a signal transduction pathway of interest.}, year = {2014}, eissn = {1932-6203}, orcid-numbers = {Tubak, Vilmos/0000-0002-6141-3920} }