TY - JOUR AU - Horváth, Ádám AU - Steib, Anita AU - Nehr-Majoros, Andrea Kinga AU - Kántás, Boglárka AU - Király, Ágnes AU - Racskó, Márk AU - Tóth, Balázs István AU - Szánti-Pintér, Eszter AU - Kudová, Eva AU - Skodáné Földes, Rita AU - Helyes, Zsuzsanna AU - Szőke, Éva TI - Anti-Nociceptive Effects of Sphingomyelinase and Methyl-Beta-Cyclodextrin in the Icilin-Induced Mouse Pain Model JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 9 PG - 13 SN - 1661-6596 DO - 10.3390/ijms25094637 UR - https://m2.mtmt.hu/api/publication/34824919 ID - 34824919 AB - The thermo- and pain-sensitive Transient Receptor Potential Melastatin 3 and 8 (TRPM3 and TRPM8) ion channels are functionally associated in the lipid rafts of the plasma membrane. We have already described that cholesterol and sphingomyelin depletion, or inhibition of sphingolipid biosynthesis decreased the TRPM8 but not the TRPM3 channel opening on cultured sensory neurons. We aimed to test the effects of lipid raft disruptors on channel activation on TRPM3- and TRPM8-expressing HEK293T cells in vitro, as well as their potential analgesic actions in TRPM3 and TRPM8 channel activation involving acute pain models in mice. CHO cell viability was examined after lipid raft disruptor treatments and their effects on channel activation on channel expressing HEK293T cells by measurement of cytoplasmic Ca2+ concentration were monitored. The effects of treatments were investigated in Pregnenolone-Sulphate-CIM-0216-evoked and icilin-induced acute nocifensive pain models in mice. Cholesterol depletion decreased CHO cell viability. Sphingomyelinase and methyl-beta-cyclodextrin reduced the duration of icilin-evoked nocifensive behavior, while lipid raft disruptors did not inhibit the activity of recombinant TRPM3 and TRPM8. We conclude that depletion of sphingomyelin or cholesterol from rafts can modulate the function of native TRPM8 receptors. Furthermore, sphingolipid cleavage provided superiority over cholesterol depletion, and this method can open novel possibilities in the management of different pain conditions. LA - English DB - MTMT ER - TY - JOUR AU - Dolejší, Eva AU - Szánti-Pintér, Eszter AU - Chetverikov, Nikolai AU - Nelic, Dominik AU - Randáková, Alena AU - Doležal, Vladimír AU - Kudová, Eva AU - Jakubík, Jan TI - Neurosteroids and steroid hormones are allosteric modulators of muscarinic receptors JF - NEUROPHARMACOLOGY J2 - NEUROPHARMACOLOGY VL - 199 PY - 2021 SN - 0028-3908 DO - 10.1016/j.neuropharm.2021.108798 UR - https://m2.mtmt.hu/api/publication/32263040 ID - 32263040 LA - English DB - MTMT ER - TY - JOUR AU - Dolejší, Eva AU - Chetverikov, Nikolai AU - Szánti-Pintér, Eszter AU - Nelic, Dominik AU - Randáková, Alena AU - Doležal, Vladimír AU - El-Fakahany, Esam E. AU - Kudová, Eva AU - Jakubík, Jan TI - Neuroactive steroids, WIN-compounds and cholesterol share a common binding site on muscarinic acetylcholine receptors JF - BIOCHEMICAL PHARMACOLOGY J2 - BIOCHEMIC PHARMACOL VL - 192 PY - 2021 SN - 0006-2952 DO - 10.1016/j.bcp.2021.114699 UR - https://m2.mtmt.hu/api/publication/32263006 ID - 32263006 LA - English DB - MTMT ER - TY - JOUR AU - Horváth, Ádám AU - Biró-Sütő, Tünde AU - Kántás, Boglárka AU - Payrits, Maja AU - Skodáné Földes, Rita AU - Szánti-Pintér, Eszter AU - Helyes, Zsuzsanna AU - Szőke, Éva TI - Antinociceptive Effects of Lipid Raft Disruptors, a Novel Carboxamido-Steroid and Methyl β-Cyclodextrin, in Mice by Inhibiting Transient Receptor Potential Vanilloid 1 and Ankyrin 1 Channel Activation JF - FRONTIERS IN PHYSIOLOGY J2 - FRONT PHYSIOL VL - 11 PY - 2020 PG - 9 SN - 1664-042X DO - 10.3389/fphys.2020.559109 UR - https://m2.mtmt.hu/api/publication/31608849 ID - 31608849 N1 - * Megosztott szerzőség LA - English DB - MTMT ER - TY - JOUR AU - Herman, Bianka Edina AU - Gardi, János AU - Julesz, János AU - Tömböly, Csaba AU - Szánti-Pintér, Eszter AU - Fehér, Klaudia AU - Skodáné Földes, Rita AU - Szécsi, Mihály TI - Steroidal ferrocenes as potential enzyme inhibitors of the estrogen biosynthesis JF - BIOLOGIA FUTURA J2 - BIOL FUTURA VL - 71 PY - 2020 IS - 3 SP - 249 EP - 264 PG - 16 SN - 2676-8615 DO - 10.1007/s42977-020-00023-7 UR - https://m2.mtmt.hu/api/publication/31365295 ID - 31365295 LA - English DB - MTMT ER - TY - JOUR AU - Szánti-Pintér, Eszter AU - Skodáné Földes, Rita TI - Application of Ionic Liquids in Synthetic Procedures Leading to Pharmaceutically Active Organic Compounds JF - CURRENT GREEN CHEMISTRY J2 - CURR GREEN CHEM VL - 5 PY - 2018 IS - 1 SP - 4 EP - 21 PG - 18 SN - 2213-3461 DO - 10.2174/2213346105666180220121503 UR - https://m2.mtmt.hu/api/publication/31628662 ID - 31628662 AB - Due to an increasing pressure on the chemical society to consider environmental aspects of chemical production, the development of sustainable methodologies has been in the focus of research for years. Ionic Liquids (ILs) have numerous favorable properties: outstanding chemical, thermal and electrochemical stability, non-flammability, negligible volatility, excellent solvation ability and great variability of cation-anion pairs and structure that render them useful materials in green reactions. In pharmaceutics, they may find wide application in the synthesis, extraction and purification of bioactive compounds or may serve as pharmaceutical ingredients themselves. The review focusses on the use of ILs in the synthesis of pharmaceutically active compounds as reaction media, catalyst or catalyst support. ILs can be the efficient solvents as they are known to stabilize reactive intermediates, besides, they facilitate catalyst reuse in e.g. transition metal-catalyzed reactions. Acidic or basic ILs may serve as recyclable catalysts themselves. Other representatives may contain functional groups enabling their application as organocatalysts. Immobilization of catalytically active species on a Supported Ionic Liquid Phase (SILP) as solid material may further enhance catalytic efficiency. The review shows examples for the application of the methodologies mentioned above in the synthesis of biologically active molecules. LA - English DB - MTMT ER - TY - BOOK AU - Szánti-Pintér, Eszter AU - Ispán, Dávid AU - Maksó, Lilla AU - Skodáné Földes, Rita TI - Application of ionic liquids in the synthesis of steroid derivatives PY - 2018 UR - https://m2.mtmt.hu/api/publication/31396002 ID - 31396002 LA - English DB - MTMT ER - TY - JOUR AU - Sághy, Éva AU - Payrits, Maja AU - Biro-Suto, T AU - Skodáné Földes, Rita AU - Szánti-Pintér, Eszter AU - Erostyák, János AU - Makkai, Géza AU - Sétáló, György (ifj.) AU - Kollár, László AU - Kőszegi, Tamás AU - Jakabfi-Csepregi, Rita AU - Szolcsányi, János AU - Helyes, Zsuzsanna AU - Szőke, Éva TI - Carboxamido steroids inhibit the opening properties of Transient Receptor Potential ion channels by lipid raft modulation. JF - JOURNAL OF LIPID RESEARCH J2 - J LIPID RES VL - 59 PY - 2018 IS - 10 SP - 1851 EP - 1863 PG - 13 SN - 0022-2275 DO - 10.1194/jlr.M084723 UR - https://m2.mtmt.hu/api/publication/3402940 ID - 3402940 N1 - * Megosztott szerzőség AB - Transient Receptor Potential (TRP) cation channels, like the TRP Vanilloid 1 and TRP Ankyrin 1 (TRPV1 and TRPA1) are expressed on primary sensory neurons. These thermosensor channels play role in pain processing. We provided evidence that lipid raft disruption influenced the TRP channel activation and a carboxamido-steroid compound (C1) inhibited TRPV1 activation. Therefore, our aim was to investigate whether this compound exerts its effect through lipid raft disruption and the steroid backbone (C3) or altered position of the carboxamido group (C2) influence the inhibitory action by measuring Ca2+-transients on isolated neurons and calcium-uptake on receptor-expressing CHO cells. Membrane cholesterol content was measured by filipin staining and membrane polarisation by fluorescence spectroscopy. Both the percentage of responsive cells and the magnitude of the intracellular Ca2+-enhancement evoked by the TRPV1 agonist capsaicin were significantly inhibited after C1 and C2 incubation, but not after C3 administration. C1 was able to reduce other TRP channel activation as well. The compounds induced cholesterol depletion in CHO cells, but only C1 induced changes in membrane polarisation. The inhibitory action of the compounds on TRP channel activation develops by lipid raft disruption, and the presence and the position of the carboxamido group is essential. LA - English DB - MTMT ER - TY - JOUR AU - Ispán, Dávid AU - Szánti-Pintér, Eszter AU - Papp, Máté AU - J, Wouters AU - N, Tumanov AU - Zsirka, Balázs AU - Gömöry, Ágnes AU - Kollár, László AU - Skodáné Földes, Rita TI - The use of switchable polarity solvents for the synthesis of 16-arylidene steroids via Claisen-Schmidt condensation JF - EUROPEAN JOURNAL OF ORGANIC CHEMISTRY J2 - EUR J ORG CHEM VL - 2018 PY - 2018 IS - 24 SP - 3236 EP - 3244 PG - 9 SN - 1434-193X DO - 10.1002/ejoc.201800356 UR - https://m2.mtmt.hu/api/publication/3365577 ID - 3365577 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office [OTKA 116727, 120014]; uNKP-17-2 New National Excellence Program of the Ministry of Human Capacities Funding text: The support of the National Research, Development and Innovation Office (OTKA 116727 and 120014) is acknowledged. This work is supported by the uNKP-17-2 New National Excellence Program of the Ministry of Human Capacities. X-ray diffraction data were recorded within the Plateforme de caracterisation PC2 at UNamur. LA - English DB - MTMT ER - TY - JOUR AU - Szánti-Pintér, Eszter AU - L, Maksó AU - Gömöry, Ágnes AU - J, Wouters AU - Herman, Bianka Edina AU - Szécsi, Mihály AU - Mikle, Gábor AU - Kollár, László AU - Skodáné Földes, Rita TI - Synthesis of 16α-amino-pregnenolone derivatives via ionic liquid-catalyzed aza-Michael addition and their evaluation as C17,20-lyase inhibitors JF - STEROIDS J2 - STEROIDS VL - 123 PY - 2017 SP - 61 EP - 66 PG - 6 SN - 0039-128X DO - 10.1016/j.steroids.2017.05.006 UR - https://m2.mtmt.hu/api/publication/3227244 ID - 3227244 N1 - WoS:hiba:000403131400009 2019-12-12 23:24 cím nem egyezik Funding Agency and Grant Number: National Research, Development and Innovation Office [OTKA 120014, K113177] Funding text: The support of the National Research, Development and Innovation Office (OTKA 120014 and K113177) is acknowledged. X-ray diffraction data were recorded within the Plateforme de caracterisation PC2 at UNamur. LA - English DB - MTMT ER -