TY - JOUR AU - Balázs, Gergely AU - Balajthy, András AU - Seri, István AU - Hegyi, Thomas AU - Ertl, Tibor AU - Szabó, Tamás AU - Röszer, Tamás AU - Papp, Ágnes AU - Balla, József AU - Gáll, Tamás AU - Balla, György TI - Prevention of Chronic Morbidities in Extremely Premature Newborns with LISA-nCPAP Respiratory Therapy and Adjuvant Perinatal Strategies JF - ANTIOXIDANTS J2 - ANTIOXIDANTS-BASEL VL - 12 PY - 2023 IS - 6 PG - 36 SN - 2076-3921 DO - 10.3390/antiox12061149 UR - https://m2.mtmt.hu/api/publication/33927937 ID - 33927937 N1 - Department of Pediatrics, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary First Department of Pediatrics, School of Medicine, Semmelweis University, Budapest, 1083, Hungary Keck School of Medicine of USC, Children’s Hospital of Los Angeles, Los Angeles, CA 90033, United States Department of Pediatrics, Division of Neonatology, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, New Brunswick, NJ 08903, United States Departments of Neonatology and Obstetrics & Gynecology, University of Pécs Medical School, Pécs, 7624, Hungary MTA-PTE Human Reproduction Scientific Research Group, University of Pécs, Pécs, 7624, Hungary Department of Internal Medicine, Division of Nephrology, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary ELKH-UD Vascular Pathophysiology Research Group, Hungarian Academy of Sciences, University of Debrecen, Debrecen, 4032, Hungary Export Date: 4 September 2023 Correspondence Address: Balla, G.; Department of Pediatrics, Hungary; email: balla@med.unideb.hu Correspondence Address: Gáll, T.; Department of Internal Medicine, Hungary; email: gall.tamas@med.unideb.hu AB - Less invasive surfactant administration techniques, together with nasal continuous airway pressure (LISA-nCPAP) ventilation, an emerging noninvasive ventilation (NIV) technique in neonatology, are gaining more significance, even in extremely premature newborns (ELBW), under 27 weeks of gestational age. In this review, studies on LISA-nCPAP are compiled with an emphasis on short- and long-term morbidities associated with prematurity. Several perinatal preventative and therapeutic investigations are also discussed in order to start integrated therapies as numerous organ-saving techniques in addition to lung-protective ventilations. Two thirds of immature newborns can start their lives on NIV, and one third of them never need mechanical ventilation. With adjuvant intervention, these ratios are expected to be increased, resulting in better outcomes. Optimized cardiopulmonary transition, especially physiologic cord clamping, could have an additively beneficial effect on patient outcomes gained from NIV. Organ development and angiogenesis are strictly linked not only in the immature lung and retina, but also possibly in the kidney, and optimized interventions using angiogenic growth factors could lead to better morbidity-free survival. Corticosteroids, caffeine, insulin, thyroid hormones, antioxidants, N-acetylcysteine, and, moreover, the immunomodulatory components of mother's milk are also discussed as adjuvant treatments, since immature newborns deserve more complex neonatal interventions. LA - English DB - MTMT ER - TY - JOUR AU - Balázs, Gergely AU - PECSI, Ivett AU - FEHER, Csilla AU - KATONA, Nora AU - KOTORMAN, Tunde AU - KOVACS-PASZTHY, Balazs AU - MARKI, Mariann AU - Pataki, István AU - Riszter, Magdolna AU - ROZSA, Timea AU - Bélteki, Gusztáv AU - KOVACS, Tamas AU - Balla, György AU - Balajthy, András TI - Comparison of flexible nasogastric tube and semi-rigid catheter during less invasive surfactant administration JF - MINERVA PEDIATRICS J2 - MINERVA PEDIATR PY - 2023 SN - 2724-5276 DO - 10.23736/S2724-5276.23.07210-5 UR - https://m2.mtmt.hu/api/publication/33927792 ID - 33927792 LA - English DB - MTMT ER - TY - JOUR AU - Ispánné Varga, Petra AU - Balajthy, András AU - Biró, Erika AU - Bíró, Bernadett AU - Reiger, Zsolt AU - Szikszay, Edit AU - Mogyorósy, Gábor AU - Káposzta, Rita Kinga AU - Szabó, Tamás TI - Multicolored MIS-C, a single-centre cohort study JF - BMC PEDIATRICS J2 - BMC PEDIATR VL - 23 PY - 2023 IS - 1 SN - 1471-2431 DO - 10.1186/s12887-023-03997-0 UR - https://m2.mtmt.hu/api/publication/33805323 ID - 33805323 LA - English DB - MTMT ER - TY - JOUR AU - Balajthy, András AU - Kovács, Panna Eszter AU - Márki, Mariann AU - Riszter, Magdolna AU - Nagy, Andrea AU - Balázs, Gergely TI - A respirációs distressz szindróma kezelésének trendjei a Debreceni Egyetem Klinikai Központjába szállított igen éretlen koraszülöttekben JF - ORVOSI HETILAP J2 - ORV HETIL VL - 164 PY - 2023 IS - 15 SP - 571 EP - 576 PG - 6 SN - 0030-6002 DO - 10.1556/650.2023.32735 UR - https://m2.mtmt.hu/api/publication/33764929 ID - 33764929 AB - Bevezetés: Fenyegető koraszülés esetén in utero transzport javasolt, ez azonban nem mindig lehetséges. A postnatalis transzport alatti ellátás jelentősen befolyásolja a szállított betegek kimenetelét. Célkitűzés: Tanulmányunk célja volt a betegek jellemzőinek, ellátásuk és a neonatalis kimenetel trendjeinek vizsgálata a 2008 és 2021 között postnatalis szállítást igénylő koraszülöttekben. Módszer: Retrospektív vizsgálatot végeztünk az írott és az elektronikus betegdokumentáció áttekintésével. A vizsgált trendeket „joinpoint” regressziós analízissel értékeltük, illetve éves százalékos változással (APC) jellemeztük. Eredmények: A vizsgálatba 177 koraszülöttet választottunk be. A szállítások száma nem szignifikáns növekvő trendet mutatott (APC = 6,8%, p = 0,087). A 60 percnél hosszabb helyszíni ellátások aránya szignifikánsan emelkedett (APC = 7,4%, p = 0,016). 2008 és 2010 között a gépi lélegeztetés alkalmazása a szállítások során emelkedett (APC = 36,4%, p = 0,578), majd a vizsgálati időszak hátralévő részében csökkenő tendenciát mutatott (APC = –7,2%, p = 0,068). A 40% feletti oxigénkoncentráció használata szignifikánsan csökkent (APC = –9,5%, p = 0,043). A 150 mg/kg-nál kisebb surfactantdózisok aránya szintén csökkenő tendenciát mutatott (APC = –7,65%, p = 0,162), míg a 180 mg/kg felettiek száma szignifikánsan emelkedett (APC = 8,5%, p = 0,031). Az újszülöttek hosszú távú kimeneti mutatói egyaránt javuló tendenciát mutattak. Megbeszélés: Vizsgálatunk során az ellátás noninvazivitás felé mutató trendjei mellett a szállított koraszülöttek javuló kimenetelét észleltük. Következtetés: Vizsgálatunk felgyorsíthatja a szállítás alatti ellátás folyamatban lévő szemléletváltását, elősegítheti a vonatkozó protokollok, illetve eljárásrendek fejlesztését, melyek együttesen javíthatják a tercier centrumon kívül született koraszülöttek életkilátásait. Orv Hetil. 2023; 164(15): 571–576. LA - Hungarian DB - MTMT ER - TY - JOUR AU - Balajthy, András AU - Balázs, Gergely AU - Kovacs, Tamas AU - Bélteki, Gusztáv TI - Synchronized intermittent mandatory ventilation with volume guarantee and pressure support in neonates: Detailed analysis of ventilator parameters JF - PEDIATRIC PULMONOLOGY J2 - PEDIATR PULM VL - 58 PY - 2023 IS - 6 SP - 1703 EP - 1710 PG - 8 SN - 8755-6863 DO - 10.1002/ppul.26384 UR - https://m2.mtmt.hu/api/publication/33719095 ID - 33719095 AB - Objective: To analyse the relationship between peak inflating pressure, expired tidal volume, respiratory rate, and inspiratory time of volume-guaranteed ventilator inflations and pressure-supported spontaneous breaths during synchronized intermittent positive pressure mode with volume guarantee and pressure support (SIMV-VG-PS) in neonates. Methods: Ventilator parameters were downloaded every second from 16 babies ventilated with SIMV-VG-PS mode using Dräger Babylog VN500 ventilators over 137 days. Transcutaneous carbon dioxide (tcCO2) data were also collected. Data were computationally analysed using Python. The average of each ventilator parameter was determined during each minute separately for ventilator inflations and for spontaneous breaths. These values were compared and their effect on tcCO2 levels was also analysed. Results: The relationship between the peak inflating pressure of the volume guaranteed inflations (PIPVG) and pressure-supported spontaneous breaths (PIPPS) was highly variable. The PIPPS/PIPVG ratio differed significantly from the value (0.66) targeted by clinicians (group median: 0.80, range: 0.50–1.00). PIPPS frequently exceeded PIPVG. When PIPPS/PIPVG was >0.66, the expired tidal volume and the rate of the pressure-supported spontaneous breaths were also significantly (p < 0.0001) higher, but there was no difference in tcCO2 levels. The flow-cycled spontaneous breaths had significantly shorter inspiratory times than ventilator inflations. Conclusions: During SIMV-VG-PS it is difficult to ensure a pressure support level proportionate to the inflating pressure of ventilator inflations and to achieve the stability of tidal volumes. LA - English DB - MTMT ER - TY - JOUR AU - Chimote, Ameet A AU - Balajthy, András AU - Arnold, Michael J AU - Newton, Hannah S AU - Hajdu, Péter Béla AU - Qualtieri, Julianne AU - Wise-Draper, Trisha AU - Conforti, Laura TI - A defect in KCa3.1 channel activity limits the ability of CD8+ T cells from cancer patients to infiltrate an adenosine-rich microenvironment. JF - SCIENCE SIGNALING J2 - SCI SIGNAL VL - 11 PY - 2018 IS - 527 SN - 1945-0877 DO - 10.1126/scisignal.aaq1616 UR - https://m2.mtmt.hu/api/publication/30588079 ID - 30588079 AB - The limited ability of cytotoxic T cells to infiltrate solid tumors hampers immune surveillance and the efficacy of immunotherapies in cancer. Adenosine accumulates in solid tumors and inhibits tumor-specific T cells. Adenosine inhibits T cell motility through the A2A receptor (A2AR) and suppression of KCa3.1 channels. We conducted three-dimensional chemotaxis experiments to elucidate the effect of adenosine on the migration of peripheral blood CD8+ T cells from head and neck squamous cell carcinoma (HNSCC) patients. The chemotaxis of HNSCC CD8+ T cells was reduced in the presence of adenosine, and the effect was greater on HNSCC CD8+ T cells than on healthy donor (HD) CD8+ T cells. This response correlated with the inability of CD8+ T cells to infiltrate tumors. The effect of adenosine was mimicked by an A2AR agonist and prevented by an A2AR antagonist. We found no differences in A2AR expression, 3',5'-cyclic adenosine monophosphate abundance, or protein kinase A type 1 activity between HNSCC and HD CD8+ T cells. We instead detected a decrease in KCa3.1 channel activity, but not expression, in HNSCC CD8+ T cells. Activation of KCa3.1 channels by 1-EBIO restored the ability of HNSCC CD8+ T cells to chemotax in the presence of adenosine. Our data highlight the mechanism underlying the increased sensitivity of HNSCC CD8+ T cells to adenosine and the potential therapeutic benefit of KCa3.1 channel activators, which could increase infiltration of these T cells into tumors. LA - English DB - MTMT ER - TY - JOUR AU - Vörös, Orsolya AU - Szilágyi, Orsolya AU - Balajthy, András AU - Somodi, Sándor AU - Panyi, György AU - Hajdu, Péter Béla TI - The C-terminal HRET sequence of Kv1.3 regulates gating rather than targeting of Kv1.3 to the plasma membrane JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 8 PY - 2018 IS - 1 PG - 14 SN - 2045-2322 DO - 10.1038/s41598-018-24159-8 UR - https://m2.mtmt.hu/api/publication/3360579 ID - 3360579 N1 - Bolyai János Fellowship, Astellas Pharma Fellowship and National Excellence Program AB - Kv1.3 channels are expressed in several cell types including immune cells, such as T lymphocytes. The targeting of Kv1.3 to the plasma membrane is essential for T cell clonal expansion and assumed to be guided by the C-terminus of the channel. Using two point mutants of Kv1.3 with remarkably different features compared to the wild-type Kv1.3 (A413V and H399K having fast inactivation kinetics and tetraethylammonium-insensitivity, respectively) we showed that both Kv1.3 channel variants target to the membrane when the C-terminus was truncated right after the conserved HRET sequence and produce currents identical to those with a full-length C-terminus. The truncation before the HRET sequence (NOHRET channels) resulted in reduced membrane-targeting but non-functional phenotypes. NOHRET channels did not display gating currents, and coexpression with wild-type Kv1.3 did not rescue the NOHRET-A413V phenotype, no heteromeric current was observed. Interestingly, mutants of wild-type Kv1.3 lacking HRET(E) (deletion) or substituted with five alanines for the HRET(E) motif expressed current indistinguishable from the wild-type. These results demonstrate that the C-terminal region of Kv1.3 immediately proximal to the S6 helix is required for the activation gating and conduction, whereas the presence of the distal region of the C-terminus is not exclusively required for trafficking of Kv1.3 to the plasma membrane. LA - English DB - MTMT ER - TY - JOUR AU - Balajthy, András AU - Hajdu, Péter Béla AU - Panyi, György AU - Varga, Zoltán TI - Sterol Regulation of Voltage-Gated K+ Channels JF - CURRENT TOPICS IN MEMBRANES J2 - CURR TOP MEMBR VL - 80 PY - 2017 SP - 255 EP - 292 PG - 38 SN - 1063-5823 DO - 10.1016/bs.ctm.2017.05.006 UR - https://m2.mtmt.hu/api/publication/3272305 ID - 3272305 LA - English DB - MTMT ER - TY - THES AU - Balajthy, András TI - A Kv1.3 ioncsatornák szterolok általi szabályozásának vizsgálata in vitro és ex vivo rendszerekben PY - 2016 UR - https://m2.mtmt.hu/api/publication/3142546 ID - 3142546 N1 - Tudományág: elméleti orvostudományok Témavezető: Hajdu Péter Béla Beadás éve: 2016 A védés időpontja: 2016-10-13 13:00 A fokozat odaítélésének dátuma: 2016-11-09 Debreceni Egyetem, Molekuláris Orvostudomány Doktori Iskola Debrecen, Magyarország LA - Hungarian DB - MTMT ER - TY - JOUR AU - Balajthy, András AU - Somodi, Sándor AU - Pethő, Z AU - Péter, Mária AU - Varga, Zoltán AU - P. Szabó, Gabriella AU - Paragh, György AU - Vigh, László AU - Panyi, György AU - Hajdu, Péter Béla TI - 7DHC-induced changes of Kv1.3 operation contributes to modified T cell function in Smith-Lemli-Opitz syndrome JF - PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY J2 - PFLUG ARCH EUR J PHY VL - 468 PY - 2016 IS - 8 SP - 1403 EP - 1418 PG - 16 SN - 0031-6768 DO - 10.1007/s00424-016-1851-4 UR - https://m2.mtmt.hu/api/publication/3099238 ID - 3099238 AB - In vitro manipulation of membrane sterol level affects the regulation of ion channels and consequently certain cellular functions; however, a comprehensive study that confirms the pathophysiological significance of these results is missing. The malfunction of 7-dehydrocholesterol (7DHC) reductase in Smith-Lemli-Opitz syndrome (SLOS) leads to the elevation of the 7-dehydrocholesterol level in the plasma membrane. T lymphocytes were isolated from SLOS patients to assess the effect of the in vivo altered membrane sterol composition on the operation of the voltage-gated Kv1.3 channel and the ion channel-dependent mitogenic responses. We found that the kinetic and equilibrium parameters of Kv1.3 activation changed in SLOS cells. Identical changes in Kv1.3 operation were observed when control/healthy T cells were loaded with 7DHC. Removal of the putative sterol binding sites on Kv1.3 resulted in a phenotype that was not influenced by the elevation in membrane sterol level. Functional assays exhibited impaired activation and proliferation rate of T cells probably partially due to the modified Kv1.3 operation. We concluded that the altered membrane sterol composition hindered the operation of Kv1.3 as well as the ion channel-controlled T cell functions. © 2016, Springer-Verlag Berlin Heidelberg. LA - English DB - MTMT ER -