TY - JOUR AU - Gémes, Nikolett AU - Makra, Zsófia AU - Neuperger, Patricia AU - Szabó, Enikő AU - Balog, József Ágoston AU - Flink, Lili Borbála AU - Kari, Beáta AU - Hackler, László AU - Puskás, László AU - Kanizsai, Iván AU - Szebeni, Gábor TI - A cytotoxic survey on 2-amino-1H-imidazol based synthetic marine sponge alkaloid analogues JF - DRUG DEVELOPMENT RESEARCH J2 - DRUG DEVELOP RES VL - 83 PY - 2022 IS - 8 SP - 1906 EP - 1922 PG - 17 SN - 0272-4391 DO - 10.1002/ddr.22006 UR - https://m2.mtmt.hu/api/publication/33211172 ID - 33211172 N1 - Funding Agency and Grant Number: National Research, Development, and Innovation Office (NKFI), Hungary [2020-1.1.6-JOVO-2021-00003, 142877 FK22]; New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund [UNKP-22-5 -SZTE-535]; [BO/00582/22/8] Funding text: This research was funded by the 2020-1.1.6-JOVO-2021-00003 and 142877 FK22 (GJS) grant from the National Research, Development, and Innovation Office (NKFI), Hungary. This work was supported by the UNKP-22-5 -SZTE-535 (GJS) New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund. This work was supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences BO/00582/22/8 (GJS). AB - Here, we describe the synthesis and biologic activity evaluation of 20 novel synthetic marine sponge alkaloid analogues with 2-amino-1H-imidazol (2-AI) core. Cytotoxicity was tested on murine 4T1 breast cancer, A549 human lung cancer, and HL-60 human myeloid leukemia cells by the resazurin assay. A total of 18 of 20 compounds showed cytotoxic effect on the cancer cell lines with different potential. Viability of healthy human fibroblasts and peripheral blood mononuclear cells upon treatment was less hampered compared to cancer cell lines supporting tumor cell specific cytotoxicity of our compounds. The most cytotoxic compounds resulted the following IC50 values 28: 2.91 µM on HL-60 cells, and 29: 3.1 µM on 4T1 cells. The A549 cells were less sensitive to the treatments with IC50 15 µM for both 28 and 29. Flow cytometry demonstrated the apoptotic effect of the most active seven compounds inducing phosphatidylserine exposure and sub-G1 fragmentation of nuclear DNA. Cell cycle arrest was also observed. Four compounds caused depolarization of the mitochondrial membrane potential as an early event of apoptosis. Two lead compounds inhibited tumor growth in vivo in the 4T1 triple negative breast cancer and A549 human lung adenocarcinoma xenograft models. Novel marine sponge alkaloid analogues are demonstrated as potential anticancer agents for further development. LA - English DB - MTMT ER - TY - JOUR AU - Tajti, Ádám AU - Szabó, Kármen AU - Popovics-Tóth, Nóra AU - Iskanderov, Javad AU - Perdih, Franc AU - Hackler, László AU - Kari, Beáta AU - Puskás, László AU - Bálint, Erika TI - PMDTA-catalyzed multicomponent synthesis and biological activity of 2-amino-4 H -chromenes containing a phosphonate or phosphine oxide moiety JF - ORGANIC & BIOMOLECULAR CHEMISTRY J2 - ORG BIOMOL CHEM VL - 19 PY - 2021 SP - 6883 EP - 6891 PG - 9 SN - 1477-0520 DO - 10.1039/D1OB01204E UR - https://m2.mtmt.hu/api/publication/32129515 ID - 32129515 N1 - Funding Agency and Grant Number: Hungarian Research Development and Innovation OfficeNational Research, Development & Innovation Office (NRDIO) - Hungary [FK123961]; bilateral Hungarian-Slovenian Science and Technology Cooperation project [2018-2.1.11-TET-SI-2018-00008]; Slovenian Research AgencySlovenian Research Agency - Slovenia [P1-0230-0175]; Hungarian Academy of SciencesHungarian Academy of Sciences [BO/00278/17/7]; New National Excellence Program of the Ministry of Human Capacities [uNKP-20-5-BME-288]; Servier-Beregi PhD Research Fellowship Funding text: The project was supported by the Hungarian Research Development and Innovation Office (FK123961), the bilateral Hungarian-Slovenian Science and Technology Cooperation project (2018-2.1.11-TET-SI-2018-00008) and the Slovenian Research Agency (P1-0230-0175). N. P.-T. was supported by the Servier-Beregi PhD Research Fellowship. E. B. was supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/00278/17/7) and the uNKP-20-5-BME-288 New National Excellence Program of the Ministry of Human Capacities. F. P. thanks the EN-FIST Centre of Excellence, Slovenia, for the use of the Supernova diffractometer. LA - English DB - MTMT ER - TY - JOUR AU - Balog, József Ágoston AU - Honti, Viktor AU - Kurucz, Judit Éva AU - Kari, Beáta AU - Puskás, László AU - Andó, István AU - Szebeni, Gábor TI - Immunoprofiling of Drosophila Hemocytes by Single-cell Mass Cytometry JF - GENOMICS PROTEOMICS & BIOINFORMATICS J2 - GENOM PROTEOM BIOINF VL - 19 PY - 2021 IS - 2 SP - 243 EP - 252 PG - 10 SN - 1672-0229 DO - 10.1016/j.gpb.2020.06.022 UR - https://m2.mtmt.hu/api/publication/31940120 ID - 31940120 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office, HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [GINOP-2.3.2-15-2016-00001, GINOP-2.3.2-152016-00030, GINOP-2.3.2-15-2016-00035, NKFI NN118207, NKFI K120142, NKFI 120140, OTKA K-131484]; New National Excellence Program of the Ministry for Innovation and Technology, Hungary [UNKP-19-4-SZTE-36]; Janos Bolyai Research Scholarship of the Hungarian Academy of SciencesHungarian Academy of Sciences [BO/00139/17/8] Funding text: This work was supported by the grants from the National Research, Development and Innovation Office, Hungary (Grant Nos. GINOP-2.3.2-15-2016-00001, GINOP-2.3.2-152016-00030 to LGP, GINOP-2.3.2-15-2016-00035 to E ' K, NKFI NN118207 and NKFI K120142 to IA, NKFI 120140 to E ' K, and OTKA K-131484 to VH). Ga ' bor J. Szebeni was supported by the New National Excellence Program of the Ministry for Innovation and Technology, Hungary (Grant No. UNKP-19-4-SZTE-36) and by the Ja ' nos Bolyai Research Scholarship of the Hungarian Academy of Sciences (Grant No. BO/00139/17/8). We are grateful to Mrs. Olga Kovalcsik for the technical help. LA - English DB - MTMT ER - TY - JOUR AU - Tripolszky, Anna AU - Tóth, Emese AU - Szabó, Pál Tamás AU - Hackler, László AU - Kari, Beáta AU - Puskás, László AU - Bálint, Erika TI - Synthesis and In Vitro Cytotoxicity and Antibacterial Activity of Novel 1,2,3-Triazol-5-yl-Phosphonates JF - MOLECULES J2 - MOLECULES VL - 25 PY - 2020 IS - 11 PG - 16 SN - 1420-3049 DO - 10.3390/molecules25112643 UR - https://m2.mtmt.hu/api/publication/31346366 ID - 31346366 N1 - Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, Budapest, H-1521, Hungary MS Metabolomics Laboratory, Instrumentation Center, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok krt. 2., Budapest, 1117, Hungary Avidin Ltd., Alsó kiköto sor 11/D, Szeged, H-6726, Hungary Cited By :6 Export Date: 5 October 2022 CODEN: MOLEF Correspondence Address: Bálint, E.; Department of Organic Chemistry and Technology, Hungary; email: ebalint@mail.bme.hu Correspondence Address: Puskás, L.G.; Avidin Ltd., Alsó kiköto sor 11/D, Hungary; email: laszlo@avidinbiotech.com LA - English DB - MTMT ER - TY - JOUR AU - Szebeni, Gábor AU - Nagy, Lajos I. AU - Magyariné, Berkó Anikó AU - Nagyné Hoffmann, Alexandra AU - Fehér, Liliána Z. AU - Bagyánszki, Mária AU - Kari, Beáta AU - Balog, József Ágoston AU - Hackler, László AU - Kanizsai, Iván AU - Pósa, Anikó AU - Varga, Csaba AU - Puskás, László TI - The Anti-Inflammatory Role of Mannich Curcuminoids; Special Focus on Colitis JF - MOLECULES J2 - MOLECULES VL - 24 PY - 2019 IS - 8 PG - 14 SN - 1420-3049 DO - 10.3390/molecules24081546 UR - https://m2.mtmt.hu/api/publication/30644621 ID - 30644621 N1 - Laboratory of Functional Genomics, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62, Szeged, H-6726, Hungary Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary Avidin Ltd., Alsó kiköt o sor 11/D, Szeged, H-6726, Hungary AstridBio Technologies Ltd., Alsó kiköt o sor 11/D, Szeged, H-6726, Hungary Department of Physiology, Anatomy and Neuroscience, Interdisciplinary Excellence Centre, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary Cited By :1 Export Date: 28 August 2019 CODEN: MOLEF Correspondence Address: Puskás, L.G.; Laboratory of Functional Genomics, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62, Hungary; email: laszlo@avidinbiotech.com Funding details: Emberi Eroforrások Minisztériuma, UNKP-18-4-SZTE-73 Funding details: Magyar Tudományos Akadémia, BO/00139/17/8 Funding details: Office of Research, Innovation and Economic Development, California State Polytechnic University, Pomona Funding text 1: Funding: This research was supported by the following grants: 2018-1.3.1-VKE-2018-00018, GINOP-2.3.2-15 -2016-00030 and GINOP-2.3.2-15-2016-00001 by the National Research, Development and Innovation Office. The Ministry of Human Capacities, Hungary grant 20391-3/2018/FEKUSTRAT is acknowledged. Gábor J. Szebeni was supported by the UNKP-18-4 New National Excellence Program of the Ministry of Human Capacities (UNKP-18-4-SZTE-73) and János Bolyai by the Research Scholarship of the Hungarian Academy of Sciences (BO/00139/17/8). Laboratory of Functional Genomics, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62, Szeged, H-6726, Hungary Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary Avidin Ltd., Alsó kiköt o sor 11/D, Szeged, H-6726, Hungary AstridBio Technologies Ltd., Alsó kiköt o sor 11/D, Szeged, H-6726, Hungary Department of Physiology, Anatomy and Neuroscience, Interdisciplinary Excellence Centre, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary Cited By :2 Export Date: 30 January 2020 CODEN: MOLEF Correspondence Address: Puskás, L.G.; Laboratory of Functional Genomics, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62, Hungary; email: laszlo@avidinbiotech.com Laboratory of Functional Genomics, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62, Szeged, H-6726, Hungary Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary Avidin Ltd., Alsó kiköt o sor 11/D, Szeged, H-6726, Hungary AstridBio Technologies Ltd., Alsó kiköt o sor 11/D, Szeged, H-6726, Hungary Department of Physiology, Anatomy and Neuroscience, Interdisciplinary Excellence Centre, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary Cited By :8 Export Date: 22 November 2020 CODEN: MOLEF Correspondence Address: Puskás, L.G.; Laboratory of Functional Genomics, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62, Hungary; email: laszlo@avidinbiotech.com Chemicals/CAS: curcumin, 458-37-7; Anti-Inflammatory Agents; Curcumin; Interleukin-4; Interleukin-6; NF-kappa B; Tumor Necrosis Factor-alpha Funding details: Emberi Eroforrások Minisztériuma, EMMI, UNKP-18-4-SZTE-73 Funding details: Magyar Tudományos Akadémia, MTA, BO/00139/17/8 Funding details: National Research, Development and Innovation Office Funding text 1: Funding: This research was supported by the following grants: 2018-1.3.1-VKE-2018-00018, GINOP-2.3.2-15 -2016-00030 and GINOP-2.3.2-15-2016-00001 by the National Research, Development and Innovation Office. The Ministry of Human Capacities, Hungary grant 20391-3/2018/FEKUSTRAT is acknowledged. Gábor J. Szebeni was supported by the UNKP-18-4 New National Excellence Program of the Ministry of Human Capacities (UNKP-18-4-SZTE-73) and János Bolyai by the Research Scholarship of the Hungarian Academy of Sciences (BO/00139/17/8). Laboratory of Functional Genomics, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62, Szeged, H-6726, Hungary Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary Avidin Ltd., Alsó kiköt o sor 11/D, Szeged, H-6726, Hungary AstridBio Technologies Ltd., Alsó kiköt o sor 11/D, Szeged, H-6726, Hungary Department of Physiology, Anatomy and Neuroscience, Interdisciplinary Excellence Centre, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary Cited By :9 Export Date: 10 January 2021 CODEN: MOLEF Correspondence Address: Puskás, L.G.; Laboratory of Functional Genomics, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62, Hungary; email: laszlo@avidinbiotech.com Chemicals/CAS: curcumin, 458-37-7; Anti-Inflammatory Agents; Curcumin; Interleukin-4; Interleukin-6; NF-kappa B; Tumor Necrosis Factor-alpha Funding details: Emberi Eroforrások Minisztériuma, EMMI, UNKP-18-4-SZTE-73 Funding details: Magyar Tudományos Akadémia, MTA, BO/00139/17/8 Funding details: National Research, Development and Innovation Office Funding text 1: Funding: This research was supported by the following grants: 2018-1.3.1-VKE-2018-00018, GINOP-2.3.2-15 -2016-00030 and GINOP-2.3.2-15-2016-00001 by the National Research, Development and Innovation Office. The Ministry of Human Capacities, Hungary grant 20391-3/2018/FEKUSTRAT is acknowledged. Gábor J. Szebeni was supported by the UNKP-18-4 New National Excellence Program of the Ministry of Human Capacities (UNKP-18-4-SZTE-73) and János Bolyai by the Research Scholarship of the Hungarian Academy of Sciences (BO/00139/17/8). Laboratory of Functional Genomics, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62, Szeged, H-6726, Hungary Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary Avidin Ltd., Alsó kiköt o sor 11/D, Szeged, H-6726, Hungary AstridBio Technologies Ltd., Alsó kiköt o sor 11/D, Szeged, H-6726, Hungary Department of Physiology, Anatomy and Neuroscience, Interdisciplinary Excellence Centre, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary Cited By :10 Export Date: 21 January 2021 CODEN: MOLEF Correspondence Address: Puskás, L.G.; Laboratory of Functional Genomics, Temesvári krt. 62, Hungary; email: laszlo@avidinbiotech.com Laboratory of Functional Genomics, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62, Szeged, H-6726, Hungary Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary Avidin Ltd., Alsó kiköt o sor 11/D, Szeged, H-6726, Hungary AstridBio Technologies Ltd., Alsó kiköt o sor 11/D, Szeged, H-6726, Hungary Department of Physiology, Anatomy and Neuroscience, Interdisciplinary Excellence Centre, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary Cited By :10 Export Date: 10 February 2021 CODEN: MOLEF Correspondence Address: Puskás, L.G.; Laboratory of Functional Genomics, Temesvári krt. 62, Hungary; email: laszlo@avidinbiotech.com Laboratory of Functional Genomics, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62, Szeged, H-6726, Hungary Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary Avidin Ltd., Alsó kiköt o sor 11/D, Szeged, H-6726, Hungary AstridBio Technologies Ltd., Alsó kiköt o sor 11/D, Szeged, H-6726, Hungary Department of Physiology, Anatomy and Neuroscience, Interdisciplinary Excellence Centre, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary Cited By :10 Export Date: 12 March 2021 CODEN: MOLEF Correspondence Address: Puskás, L.G.; Laboratory of Functional Genomics, Temesvári krt. 62, Hungary; email: laszlo@avidinbiotech.com Chemicals/CAS: curcumin, 458-37-7; Anti-Inflammatory Agents; Curcumin; Interleukin-4; Interleukin-6; NF-kappa B; Tumor Necrosis Factor-alpha Funding details: Magyar Tudományos Akadémia, MTA, BO/00139/17/8 Funding details: Emberi Eroforrások Minisztériuma, EMMI, UNKP-18-4-SZTE-73 Funding details: National Research, Development and Innovation Office Funding text 1: Funding: This research was supported by the following grants: 2018-1.3.1-VKE-2018-00018, GINOP-2.3.2-15 -2016-00030 and GINOP-2.3.2-15-2016-00001 by the National Research, Development and Innovation Office. The Ministry of Human Capacities, Hungary grant 20391-3/2018/FEKUSTRAT is acknowledged. Gábor J. Szebeni was supported by the UNKP-18-4 New National Excellence Program of the Ministry of Human Capacities (UNKP-18-4-SZTE-73) and János Bolyai by the Research Scholarship of the Hungarian Academy of Sciences (BO/00139/17/8). AB - The incidence of inflammatory bowel disease (IBD) increases gradually in Western countries with high need for novel therapeutic interventions. Mannich curcuminoids, C142 or C150 synthetized in our laboratory, have been tested for anti-inflammatory activity in a rat model of TNBS (2,4,6-trinitrobenzenesulphonic acid) induced colitis. Treatment with C142 or C150 reduced leukocyte infiltration to the submucosa and muscular propria of the inflamed gut. C142 or C150 rescued the loss of body weight and C150 decreased the weight of standard colon preparations proportional with 20% less tissue oedema. Both C142 and C150 curcumin analogues caused 25% decrease in the severity of colonic inflammation and haemorrhagic lesion size. Colonic MPO (myeloperoxidase) enzyme activity as an indicator of intense neutrophil infiltration was 50% decreased either by C142 or C150 Mannich curcuminoids. Lipopolysaccharide (LPS) co-treatment with Mannich curcuminoids inhibited NF-B (nuclear factor kappa B) activity on a concentration-dependent manner in an NF-B-driven luciferase expressing reporter cell line. Co-treatment with LPS and curcuminoids, C142 or C150, resulted in NF-B inhibition with 3.57 M or 1.6 M half maximal effective concentration (EC50) values, respectively. C150 exerted a profound inhibition of the expression of inflammatory cytokines, tumor necrosis factor- (TNF-), interleukin-6 (IL-6), and interleukin-4 (IL-4) in human PBMCs (peripheral blood mononuclear cells) upon LPS stimulus. Mannich curcuminoids reported herein possess a powerful anti-inflammatory activity. LA - English DB - MTMT ER - TY - THES AU - Kari, Beáta TI - A veleszületett immunitás vizsgálata Drosophila melanogasterben PB - Szegedi Tudományegyetem (SZTE) PY - 2016 SP - 80 DO - 10.14232/phd.3110 UR - https://m2.mtmt.hu/api/publication/3196422 ID - 3196422 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Vilmos, Péter AU - Kristó, Ildikó AU - Szikora, Szilárd AU - Jankovics, Ferenc AU - Lukácsovich, T AU - Kari, Beáta AU - Erdélyi, Miklós TI - The actin-binding ERM protein Moesin directly regulates spindle assembly and function during mitosis JF - CELL BIOLOGY INTERNATIONAL J2 - CELL BIOL INT VL - 40 PY - 2016 IS - 6 SP - 696 EP - 707 PG - 12 SN - 1065-6995 DO - 10.1002/cbin.10607 UR - https://m2.mtmt.hu/api/publication/3071742 ID - 3071742 N1 - Biological Research Center of the Hungarian Academy of Sciences, Temesvári krt. 62, Szeged, 6726, Hungary Department of Developmental and Cell Biology, University of California Irvine, Irvine, CA 92697, United States Cited By :3 Export Date: 20 April 2021 CODEN: CBIIE Correspondence Address: Vilmos, P.; Biological Research Center of the Hungarian Academy of Sciences, Temesvári krt. 62, Hungary; email: vilmos.peter@brc.mta.hu AB - Ezrin-Radixin-Moesin proteins are highly conserved, actin-binding cytoskeletal proteins that play an essential role in microvilli formation, T-cell activation, and tumor metastasis by linking actin filaments to the plasma membrane. Recent studies demonstrated that the only Ezrin-Radixin-Moesin protein of Drosophila melanogaster, Moesin, is involved in mitotic spindle function through stabilizing cell shape and microtubules at the cell cortex. We previously observed that Moesin localizes to the mitotic spindle; hence, we tested for the biological significance of this surprising localization and investigated whether it plays a direct role in spindle function. To separate the cortical and spindle functions of Moesin during mitosis we combined cell biological and genetic methods. We used early Drosophila embryos, in which mitosis occurs in the absence of a cell cortex, and found in vivo evidence for the direct requirement of Moesin in mitotic spindle assembly and function. We also found that the accumulation of Moesin precedes the construction of the microtubule spindle, and the fusiform structure formed by Moesin persists even after the microtubules have disassembled. © 2016 International Federation for Cell Biology. LA - English DB - MTMT ER - TY - JOUR AU - Kari, Beáta AU - Csordás, Gábor AU - Honti, Viktor AU - Cinege, Gyöngyi Ilona AU - Williams, MJ AU - Andó, István AU - Kurucz, Judit Éva TI - The raspberry Gene Is Involved in the Regulation of the Cellular Immune Response in Drosophila melanogaster JF - PLOS ONE J2 - PLOS ONE VL - 11 PY - 2016 IS - 3 PG - 13 SN - 1932-6203 DO - 10.1371/journal.pone.0150910 UR - https://m2.mtmt.hu/api/publication/3045263 ID - 3045263 N1 - Biological Research Centre of Hungarian Academy of Sciences, Immunology Unit, Institute of Genetics, Szeged, Hungary Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, Sweden Cited By :6 Export Date: 14 February 2021 CODEN: POLNC AB - Drosophila is an extremely useful model organism for understanding how innate immune mechanisms defend against microbes and parasitoids. Large foreign objects trigger a potent cellular immune response in Drosophila larva. In the case of endoparasitoid wasp eggs, this response includes hemocyte proliferation, lamellocyte differentiation and eventual encapsulation of the egg. The encapsulation reaction involves the attachment and spreading of hemocytes around the egg, which requires cytoskeletal rearrangements, changes in adhesion properties and cell shape, as well as melanization of the capsule. Guanine nucleotide metabolism has an essential role in the regulation of pathways necessary for this encapsulation response. Here, we show that the Drosophila inosine 5'-monophosphate dehydrogenase (IMPDH), encoded by raspberry (ras), is centrally important for a proper cellular immune response against eggs from the parasitoid wasp Leptopilina boulardi. Notably, hemocyte attachment to the egg and subsequent melanization of the capsule are deficient in hypomorphic ras mutant larvae, which results in a compromised cellular immune response and increased survival of the parasitoid. LA - English DB - MTMT ER - TY - CHAP AU - Kari, Beáta AU - Zsámboki, János AU - Honti, Viktor AU - Csordás, Gábor AU - Márkus, Róbert AU - Andó, István AU - Kurucz, Judit Éva ED - Horváth, József ED - Monostori, Tamás TI - A SZEPTIKUS SÉRÜLÉST KÖVETŐ IMMUNVÁLASZT SZABÁLYOZÓ GENETIKAI FAKTOROK AZONOSÍTÁSÁNAK MÓDSZEREI DROSOPHILÁBAN T2 - Tudomány a vidék mindennapjaiban PB - Szegedi Tudományegyetem Mezőgazdasági Kar CY - Hódmezővásárhely SN - 9789633062456 PY - 2013 SP - 43 EP - 47 PG - 5 UR - https://m2.mtmt.hu/api/publication/2519814 ID - 2519814 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Kari, Beáta AU - Zsámboki, János AU - Honti, Viktor AU - Csordás, Gábor AU - Márkus, Róbert AU - Andó, István AU - Kurucz, Judit Éva TI - A novel method for the identification of factors involved in host-pathogen interactions in Drosophila melanogaster. JF - JOURNAL OF IMMUNOLOGICAL METHODS J2 - J IMMUNOL METHODS VL - 398-399 PY - 2013 SP - 76 EP - 82 PG - 7 SN - 0022-1759 DO - 10.1016/j.jim.2013.09.011 UR - https://m2.mtmt.hu/api/publication/2463198 ID - 2463198 N1 - WoS:hiba:000329011500009 2019-03-03 20:54 kötet nem egyezik AB - A new method was established, standardized and validated for screening factors involved in the response to septic injury in Drosophila melanogaster. The method, based on inducing lesion by removing the tarsal segments of the first pair of legs of Drosophila adults and exposing them to different bacteria, imitates injury that often occurs in the natural habitat. The method is easy to perform, highly reproducible and suitable for large-scale genetic screens with the aim of identifying factors involved in host-pathogen interactions. The technique was validated by using mutant variations of different components of the immune response, blood clotting as well as the involvement of a number of genes known to be instrumental in the humoral and cell-mediated immune responses of Drosophila was confirmed. Moreover, the combination of the present method with antibiotic treatment allows the screening of potential antimicrobial drugs in vivo. LA - English DB - MTMT ER -