TY - JOUR AU - Tóth, Kinga AU - Tóth, Ákos AU - Kamotsay, Katalin AU - Nemeth, Viktoria AU - Szabó, Dóra TI - Population snapshot of the extended-spectrum beta-lactamase-producing Escherichia coli invasive strains isolated from a Hungarian hospital JF - ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS J2 - ANN CLIN MICROB ANTI VL - 21 PY - 2022 IS - 1 PG - 10 SN - 1476-0711 DO - 10.1186/s12941-022-00493-8 UR - https://m2.mtmt.hu/api/publication/32718790 ID - 32718790 AB - Background: This study was carried out to determine the prevalence and the genetic background of extended-spectrum beta-lactamase-producing Escherichia coli invasive isolates obtained from a tertiary-care hospital in Budapest, Hungary. Methods: Between October-November 2018, all invasive ESBL-producing E. coli isolates were collected from Central Hospital of Southern Pest. The antimicrobial susceptibility testing was performed according to the EUCAST guidelines. The possible clonal relationships were investigated by core genome (cg)MLST (SeqSphere +) using whole-genome sequencing (WGS) data of isolates obtained from Illumina 251-bp paired-end sequencing. From WGS data acquired antimicrobial resistance genes, virulence genes and replicon types were retrieved using ResFinder3.1, PlasmidFinder2.1, pMLST-2.0, VirulenceFinder2.0 and Virulence Factors Database online tools. Results: Overall, six E. coli isolates proved to be resistant to third-generation cephalosporins and ESBL-producers in the study period. Full genome sequence analysis showed that five E. coli isolates belonged to the ST131 clone: two to C1-M27 subclade with bla(CTX-M-27) and three to C2/H30Rx subclade with bla(CTX-M-15). One isolate belonged to ST1193 with bla(CTX-M-27). According to cgMLST, all C2/H30Rx isolates formed a cluster (<= 6 allele differences), while the bla(CTX-M-27)-producing C1-M27 isolates differed at least 35 alleles from each other. Both C2/H30Rx and C1-M27 ST131 isolates harbored similar antimicrobial resistance gene sets. However, only C2/H30Rx isolates had the qnrB and aac(3)-IIa. The isolates carried similar extraintestinal virulence gene set but differed in some genes encoding siderophores, protectins and toxins. Moreover, only one C2/H30Rx isolate carried salmochelin siderophore system and showed virotype B. All isolates showed resistance against ceftriaxone, cefotaxime, and ciprofloxacin, and the C2/H30Rx isolates were also resistant to gentamicin, tobramycin, and ceftazidime. Conclusions: Out of six ESBL-producing E. coli, five belonged to the ST131 clone. This study indicates, that the C2/H30Rx and C1-M27 subclades of the ST131 appear to be the dominant clones collected in a Hungarian hospital. LA - English DB - MTMT ER - TY - JOUR AU - Nagy, József Bálint AU - Balázs, Bence AU - BENMAZOUZ, ISMA AU - Gyüre, Péter AU - Kövér, László AU - Kaszab, Eszter AU - Bali, Krisztina AU - Lovas-Kiss, Ádám AU - Damjanova, Ivelina AU - Majoros, László AU - Tóth, Ákos AU - Bányai, Krisztián AU - Kardos, Gábor TI - Comparison of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolates From Rooks (Corvus frugilegus) and Contemporary Human-Derived Strains: A One Health Perspective JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 12 PY - 2022 PG - 9 SN - 1664-302X DO - 10.3389/fmicb.2021.785411 UR - https://m2.mtmt.hu/api/publication/32606927 ID - 32606927 AB - During winter, a large number of rooks gather and defecate at the park of a university clinic. We investigated the prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in these birds and compared recovered isolates with contemporary human isolates. In 2016, fecal samples were collected from 112 trap-captured rooks and investigated for presence of ESBL producers using eosin methylene blue agar supplemented by 2 mg/L cefotaxime; 2,455 contemporary human fecal samples of patients of the clinics sent for routine culturing were tested similarly. In addition, 42 ESBL-producing E. coli isolates collected during the same period from inpatients were also studied. ESBL genes were sought for by PCR and were characterized by sequencing; E. coli ST131 clones were identified. Epidemiological relatedness was determined by pulsed-field gel electrophoresis and confirmed using whole genome sequencing in selected cases. Thirty-seven (33%) of sampled rooks and 42 (1.7%) of human stools yielded ESBL-producing E coli. Dominant genes were bla(CTX-M-55) and bla(CTX-M-27) in corvid, bla(CTX-M-15) and bla(CTX-M-27) in human isolates. ST162 was common among rooks. Two rook-derived E. coli belonged to ST131 C1-M27, which was also predominant (10/42) among human fecal and (15/42) human clinical isolates. Another potential link between rooks and humans was a single ST744 rook isolate grouped with one human fecal and three clinical isolates. Despite possible contact, genotypes shared between rooks and humans were rare. Thus, rooks are important as long-distance vectors and reservoirs of ESBL-producing E. coli rather than direct sources of infections to humans in our setting. LA - English DB - MTMT ER - TY - BOOK AU - Nagy, József Bálint AU - Balázs, KOLESZÁR AU - Bence, BALÁZS AU - Róka, Eszter AU - Khayer, Bernadett AU - Tóth, Ákos AU - Erika, UNGVÁRI AU - Gábor, KARDOS TI - Investigation of carbapenemase-producing Enterobacterales in black-headed gulls (Chroicocephalus ridibundus) and the River Danube PY - 2021 UR - https://m2.mtmt.hu/api/publication/32829192 ID - 32829192 LA - English DB - MTMT ER - TY - JOUR AU - Stercz, Balázs AU - Farkas, Ferenc Balázs AU - Tóth, Ákos AU - Gajdács, Márió AU - Domokos, Judit AU - Horváth, Viola AU - Ostorházi, Eszter AU - Makra, Nóra AU - Kocsis, Béla AU - Juhász, János AU - Ligeti, Balázs AU - Pongor, Sándor AU - Szabó, Dóra TI - The influence of antibiotics on transitory resistome during gut colonization with CTX-M-15 and OXA-162 producing Klebsiella pneumoniae ST15 JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 11 PY - 2021 PG - 10 SN - 2045-2322 DO - 10.1038/s41598-021-85766-6 UR - https://m2.mtmt.hu/api/publication/31921035 ID - 31921035 LA - English DB - MTMT ER - TY - JOUR AU - Juhász, János AU - Ligeti, Balázs AU - Gajdács, Márió AU - Makra, Nóra AU - Ostorházi, Eszter AU - Farkas, Ferenc Balázs AU - Stercz, Balázs AU - Tóth, Ákos AU - Domokos, Judit AU - Pongor, Sándor AU - Szabó, Dóra TI - Colonization Dynamics of Multidrug-Resistant Klebsiella pneumoniae Are Dictated by Microbiota-Cluster Group Behavior over Individual Antibiotic Susceptibility: A Metataxonomic Analysis JF - ANTIBIOTICS J2 - ANTIBIOTICS-BASEL VL - 10 PY - 2021 IS - 3 PG - 21 SN - 2079-6382 DO - 10.3390/antibiotics10030268 UR - https://m2.mtmt.hu/api/publication/31903338 ID - 31903338 N1 - Institute of Medical Microbiology, Faculty of Medicine, Semmelweis University, Nagyvárad tér 4, Budapest, 1089, Hungary Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Práter utca 50/A, Budapest, 1083, Hungary Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, Eötvös utca 6, Szeged, 6720, Hungary Department of Bacteriology, Mycology and Parasitology, National Public Health Centre, Albert Flórián út 2-6, Budapest, 1097, Hungary Cited By :4 Export Date: 6 August 2021 Correspondence Address: Szabó, D.; Institute of Medical Microbiology, Nagyvárad tér 4, Hungary; email: szabo.dora@med.semmelweis-univ.hu LA - English DB - MTMT ER - TY - JOUR AU - Balázs, Bence AU - Tóth, Zoltán AU - Nagy, Fruzsina AU - Kovács, Renátó AU - Tóth, Hajnalka AU - Nagy, József Bálint AU - Tóth, Ákos AU - Szarka, Krisztina Zsuzsanna AU - Majoros, László AU - Kardos, Gábor TI - The Role of Uniform Meropenem Usage in Acinetobacter baumannii Clone Replacement JF - ANTIBIOTICS J2 - ANTIBIOTICS-BASEL VL - 10 PY - 2021 IS - 2 SN - 2079-6382 DO - 10.3390/antibiotics10020127 UR - https://m2.mtmt.hu/api/publication/31837646 ID - 31837646 AB - The dominant carbapenem resistant Acinetobacter baumannii harboring bla(OXA-23-like) carbapenemase was replaced by bla(OXA-40-like) carriers in a Hungarian tertiary-care center with high meropenem but relatively low imipenem use. We hypothesized that alterations in antibiotic consumption may have contributed to this switch. Our workgroup previous study examined the relation between resistance spiral and the antibiotic consumption, and the results suggest that the antibiotic usage provoked the increasing resistance in case of A. baumannii. We aimed at measuring the activity of imipenem and meropenem to compare the selection pressure exerted by the different carbapenems in time-kill assays. Strain replacement was confirmed by whole genome sequencing, core-genome multilocus sequence typing (cgMLST), and resistome analysis. Based on results of the time-kill assays, we found a significant difference between two different sequence-types (STs) in case of meropenem, but not in case of imipenem susceptibility. The newly emerged ST636 and ST492 had increased resistance level against meropenem compared to the previously dominant ST2 and ST49. On the other hand, the imipenem and colistin resistance profiles were similar. These results suggest, that the uniform meropenem usage may have contributed to A. baumannii strain replacement in our setting. LA - English DB - MTMT ER - TY - CONF AU - Gábor, Kardos AU - Tóth, Ákos AU - Benkő, Ria AU - Kristóf, Katalin AU - Ivelina, Damjanova AU - Erika, Ungvári AU - Judit, Pászti AU - Bence, Balázs AU - Nagy, József Bálint AU - Hajnalka, Tóth AU - Matuz, Mária TI - Comparison of inpatient-care antibiotic consumption patterns in three Hungarian tertiary-care university hospitals T2 - European Drug Utilisation Research Group Conference 2020 C1 - Szeged PY - 2020 SP - 78 EP - 78 PG - 1 UR - https://m2.mtmt.hu/api/publication/32006607 ID - 32006607 LA - English DB - MTMT ER - TY - JOUR AU - Gajdács, Márió AU - Pappné Ábrók, Marianna AU - Lázár, Andrea AU - Jánvári, Laura AU - Tóth, Ákos AU - Terhes, Gabriella AU - Burián, Katalin TI - Detection of VIM, NDM and OXA-48 producing carbapenem resistant Enterobacterales among clinical isolates in Southern Hungary JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 67 PY - 2020 IS - 4 SP - 209 EP - 215 PG - 7 SN - 1217-8950 DO - 10.1556/030.2020.01181 UR - https://m2.mtmt.hu/api/publication/31679098 ID - 31679098 N1 - Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, Eotvos utca 6., Szeged, 6720, Hungary Institute of Clinical Microbiology, Albert Szent-Gyorgyi Clinical Center, University of Szeged, Semmelweis utca 6., Szeged, 6725, Hungary Department of Bacteriology, Mycology and Parasitology, National Public Health Centre, Albert Florian ut 2-6., Budapest, 1097, Hungary Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged, Dom ter 10., Szeged, 6720, Hungary Cited By :3 Export Date: 29 June 2021 Correspondence Address: GAJDACS, M.; Department of Pharmacodynamics and Biopharmacy, Eotvos utca 6., Hungary; email: mariopharma92@gmail.com LA - English DB - MTMT ER - TY - JOUR AU - Gajdács, Márió AU - Németh, Anita AU - Knausz, Márta AU - Barrak, Ibrahim Ádám AU - Stájer, Anette AU - Mestyán, Gyula AU - Melegh, Szilvia Zsóka AU - Nyul, Adrienn AU - Tóth, Ákos AU - Ágoston, Zsuzsanna AU - Zsoldiné Urbán, Edit TI - Streptococcus suis: An Underestimated Emerging Pathogen in Hungary? JF - MICROORGANISMS J2 - MICROORGANISMS VL - 8 PY - 2020 IS - 9 PG - 18 SN - 2076-2607 DO - 10.3390/microorganisms8091292 UR - https://m2.mtmt.hu/api/publication/31408959 ID - 31408959 N1 - Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, Eötvös utca 6., Szeged, 6720, Hungary Institute of Medical Microbiology, Faculty of Medicine, Semmelweis University, Nagyvárad tér 4., Budapest, 1089, Hungary Microbiology Laboratory, Petz Aladár County Teaching Hospital, Vasvári Pál utca 2–4., Győr, 9023, Hungary Department of Prosthodontics, Faculty of Dentistry, University of Szeged, Tiszta Lajos körút 62–64., Szeged, 6720, Hungary Department of Periodontology, Faculty of Dentistry, University of Szeged, Tiszta Lajos körút 62–64, Szeged, 6720, Hungary Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Szigeti út 12., Pécs, 7624, Hungary Department of Bacteriology, Mycology and Parasitology, National Public Health Center, Albert Flórián út 2–6., Budapest, 1097, Hungary Department of Anaesthesiology and Intensive Therapy, Faculty of Medicine, University of Szeged, Semmelweis utca 6., Szeged, 6725, Hungary Institute of Translational Medicine, Faculty of Medicine, University of Pécs, Szigeti út 12, Pécs, 7624, Hungary Cited By :14 Export Date: 7 September 2023 Correspondence Address: Gajdács, M.; Department of Pharmacodynamics and Biopharmacy, Eötvös utca 6., Hungary; email: gajdacs.mario@pharm.u-szeged.hu Correspondence Address: Gajdács, M.; Institute of Medical Microbiology, Nagyvárad tér 4., Hungary; email: gajdacs.mario@pharm.u-szeged.hu LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Ákos AU - Makai, Attila AU - Jánvári, Laura AU - Damjanova, Ivelina AU - Gajdács, Márió AU - Zsoldiné Urbán, Edit TI - Characterization of a rare blaVIM-4 metallo-β-lactamase-producing Serratia marcescens clinical isolate in Hungary. [case report] TS - [case report] JF - HELIYON J2 - HELIYON VL - 6 PY - 2020 IS - 6 PG - 4 SN - 2405-8440 DO - 10.1016/j.heliyon.2020.e04231 UR - https://m2.mtmt.hu/api/publication/31362524 ID - 31362524 N1 - Szövegében 3 oldalnál rövidebb esetismertetés, ezért besorolása rövid közlemény az MTA V. Osztályának ajánlása alapján. (BCS, SZTE admin4) LA - English DB - MTMT ER -