TY - JOUR AU - Adle-Biassette, Homa AU - Ricci, Riccardo AU - Martin, Antoine AU - Martini, Maurizio AU - Ravegnini, Gloria AU - Kaci, Rachid AU - Gélébart, Pascal AU - Poirot, Brigitte AU - Sándor, Zsuzsa AU - Lehman-Che, Jacqueline AU - Tóth, Erika AU - Papp, Bela TI - Sarco/endoplasmic reticulum calcium ATPase 3 (SERCA3) expression in gastrointestinal stromal tumours. JF - PATHOLOGY J2 - PATHOLOGY VL - 56 PY - 2024 IS - 3 SP - 343 EP - 356 PG - 14 SN - 0031-3025 DO - 10.1016/j.pathol.2023.10.012 UR - https://m2.mtmt.hu/api/publication/34486303 ID - 34486303 AB - Accurate characterisation of gastrointestinal stromal tumours (GIST) is important for prognosis and the choice of targeted therapies. Histologically the diagnosis relies on positive immunostaining of tumours for KIT (CD117) and DOG1. Here we report that GISTs also abundantly express the type 3 Sarco/Endoplasmic Reticulum Calcium ATPase (SERCA3). SERCA enzymes transport calcium ions from the cytosol into the endoplasmic reticulum and play an important role in regulating the intensity and the periodicity of calcium-induced cell activation. GISTs from various localisations, histological and molecular subtypes or risk categories were intensely immunopositive for SERCA3 with the exception of PDGFRA-mutated cases where expression was high or moderate. Strong SERCA3 expression was observed also in normal and hyperplastic interstitial cells of Cajal. Decreased SERCA3 expression in GIST was exceptionally observed in a zonal pattern, where CD117 staining was similarly decreased, reflecting clonal heterogeneity. In contrast to GIST, SERCA3 immunostaining of spindle cell tumours and other gastrointestinal tumours resembling GIST was negative or weak. In conclusion, SERCA3 immunohistochemistry may be useful for the diagnosis of GIST with high confidence, when used as a third marker in parallel with KIT and DOG1. Moreover, SERCA3 immunopositivity may be particularly helpful in cases with negative or weak KIT or DOG1 staining, a situation that may be encountered de novo, or during the spontaneous or therapy-induced clonal evolution of GIST. LA - English DB - MTMT ER - TY - JOUR AU - Szekanecz, Zoltán AU - Besnyi, Anikó Mária AU - Kónya, Péter AU - Füri, Judit Izabella AU - Király, Edit AU - Bertalan, Éva AU - Falus, György AU - Udvardi, Beatrix AU - Kovácsné Kis, Viktória AU - Andrássy, László AU - Maros, Gyula AU - Fancsik, Tamás AU - Pethő, Zsófia AU - Gomez, Izabella Ilona AU - Horváth, Ágnes AU - Gulyás, Katalin AU - Juhász, Balázs AU - Hodosi, Katalin AU - Sándor, Zsuzsa AU - Bhattoa Harjit, Pál AU - Kovács, István János TI - Bones or Stones: How Can We Apply Geophysical Techniques in Bone Research? JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 19 PG - 16 SN - 1661-6596 DO - 10.3390/ijms251910733 UR - https://m2.mtmt.hu/api/publication/35441001 ID - 35441001 AB - Some studies have used physical techniques for the assessment of bone structure and composition. However, very few studies applied multiple techniques, such as those described below, at the same time. The aim of our study was to determine the chemical and mineralogical/organic composition of bovine tibial bone samples using geophysical/geochemical reference techniques. X-ray diffraction (XRD), thermogravimetry (TG), Fourier-transform infrared spectrometry with attenuated total reflectance accessory (FTIR-ATR), inductively coupled mass spectrometry (ICP-MS) and inductively coupled optical emission spectrometry (ICP-OES) were applied to measure the organic and inorganic composition of 14 bovine bone samples. In addition, peripheral quantitative CT (QCT) was used to assess BMD in these bones. We were able to define the total composition of the studied bone samples. ICP-OES and ICP-MS techniques were used to determine the major and trace element composition. The X-ray analysis could detect inorganic crystalline compounds of bones, such as bioapatite, and its degree of ordering, indicating whether the bones belong to a younger or older individual. The total volatile content of the samples was calculated using TG and resulted in about 35 weight% (wt%). This, together with the 65 wt% total resulting from the chemical analysis (i.e., inorganic components), yielded a total approaching 100 wt%. As a large portion of the volatile content (H2O, CO2, etc.) was liberated from the organic components and, subordinately, from bioapatite, it could be concluded that the volatile-to-solid ratio of the examined bone samples was ~35:65. The FTIR-ATR analysis revealed that the organic portion consists of collagens containing amide groups, as their typical bands (OH, CH, CO, NC) were clearly identified in the infrared spectra. Numerous parameters of bone composition correlated with BMD as determined by QCT. In conclusion, we performed a complex evaluation of bovine bones to test multiple geophysical/geochemical techniques in bone research in association with QCT bone densitometry. From a medical point of view, the composition of the studied bones could be reliably examined by these methods. LA - English DB - MTMT ER - TY - JOUR AU - Kelemen, Péter Bertalan AU - Újhelyi, Mihály AU - Pukancsik, Dávid AU - Sávolt, Ákos AU - Kovács, Eszter AU - Zaka, Z AU - Sándor, Zsuzsa AU - Mátrai, Zoltán Tamás TI - A módosított Regnault „B” emlőmegtartó műtét mint II. szintű standard onkoplasztikus emlősebészeti technika JF - ORVOSI HETILAP J2 - ORV HETIL VL - 161 PY - 2020 IS - 24 SP - 1002 EP - 1011 PG - 10 SN - 0030-6002 DO - 10.1556/650.2020.31738 UR - https://m2.mtmt.hu/api/publication/31311725 ID - 31311725 N1 - Országos Onkológiai Intézet, Daganatsebészeti Központ, Emlo-Lágyrész Daganatsebészeti Osztály, Budapest, Hungary Országos Onkológiai Intézet, Radiológiai Diagnosztikai Centrum, Budapest, Hungary Országos Onkológiai Intézet, Sugárterápiás Centrum, Budapest, Hungary Országos Onkológiai Intézet, Sebészi és Molekuláris Daganatpatológiai Centrum, Budapest, Hungary Export Date: 12 May 2023 CODEN: ORHEA Correspondence Address: Újhelyi, M.Ráth György u. 7-9., Hungary LA - Hungarian DB - MTMT ER - TY - JOUR AU - Dull, Katalin AU - Egervári, Kristóf AU - Sándor, Zsuzsa AU - Méhes, Gábor TI - Aurora Kinase B Expression in Breast Carcinoma: Cell Kinetic and Genetic Aspects JF - PATHOBIOLOGY J2 - PATHOBIOLOGY VL - 79 PY - 2012 IS - 6 SP - 314 EP - 322 PG - 9 SN - 1015-2008 DO - 10.1159/000338082 UR - https://m2.mtmt.hu/api/publication/1988097 ID - 1988097 LA - English DB - MTMT ER - TY - JOUR AU - Péntek, Márta AU - Szekanecz, Zoltán AU - Czirják, László AU - Poór, Gyula AU - Rojkovich, Bernadette AU - Polgár, Anna AU - Genti, G AU - Kiss, CG AU - Sándor, Zsuzsa AU - Májer, I AU - Brodszky, Valentin AU - Gulácsi, László TI - A betegségprogresszió hatása az egészségi állapotra, életminőségre és költségekre rheumatoid arthritisben Magyarországon. [Impact of disease progression on health status, quality of life and costs in rheumatoid arthritis in Hungary] JF - ORVOSI HETILAP J2 - ORV HETIL VL - 149 PY - 2008 IS - 16 SP - 733 EP - 741 PG - 9 SN - 0030-6002 DO - 10.1556/OH.2008.28294 UR - https://m2.mtmt.hu/api/publication/1121641 ID - 1121641 AB - Rheumatoid arthritis (RA) is a chronic, progressive polyarthritis leading to substantial disability. Standardised data on consequences of discase progression are needed for clinical assessments and also for cost-effectiveness models. Aim: To analyse the impact of disease progression on health status, disease specific quality of life and costs in Hungary. Methods: A cross-sectional survey was performed between April and August, 2004, involving consecutive RA patients of 6 hospital based rheumatology outpatient centres. Self-completed questionnaires were used to assess functional (HAQ) and health status (EQ-5D), quality of life (RAQoL). Disease activity (DAS) and costs were also surveyed, statistical analysis was performed. Results: 255 patients were involved [mean age 55.5 ± 12.3 years; disease duration 9.0 ± 9.3 years; HAQ 1.38 ± 0.76; EQ-5D 0.46 ± 0.33; RAQoL 16.2 ± 8.1; DAS 5.09 ± 1.42; costs 1,043,163 (± 844,750) HUF/patient/year, conversion 1 Euro = 250 HUF]. Correlation was significant between the parameters (p<0.01): EQ-5D index = 1.014-0.25 × HAQ-0.041 × DAS; HAQ = 0.314 + 0.065 × RAQoL. Analysis by disease severity levels (HAQ groups 0.5 difference) revealed that health status worsens (mean EQ-5D: 0.784; 0.576; 0.504; 0.3671 0.211; 0.022) and costs increase (mean 628,280; 888,187; 953,759; 1,291,218; 1,346,112; 1,371,674 HUF/patient/ year) with disease progression. Minimally important worsening of functional ability (0.25 HAQ increase) corresponds to -0.0705 EQ-5D and + 1.884 RAQoL change. Lower health status difference (EQ-5D -0.05725) was calculated in patients with lower disease activity (DAS < 5.1). Conclusions: Correlation between disease progression, health status, quality of life and costs does not differ significantly from international results. The amount of costs is much lower in all disease severity levels than in developed European countries. Our study serves baseline data for health economic analysis in RA in Hungary. LA - Hungarian DB - MTMT ER - TY - JOUR AU - Kerekes, György AU - Szekanecz, Zoltán AU - Der, H AU - Sándor, Zsuzsa AU - Lakos, G AU - Muszbek, László AU - Csípő, István AU - Sipka, Sándor AU - Seres, Ildikó AU - Paragh, György AU - Kappelmayer, János AU - Szomják, Edit AU - Veres, Katalin AU - Szegedi, Gyula AU - Shoenfeld, Y AU - Soltész, Pál TI - Endothelial dysfunction and atherosclerosis in rheumatoid arthritis: a multiparametric analysis using imaging techniques and laboratory markers of inflammation and autoimmunity. JF - JOURNAL OF RHEUMATOLOGY J2 - J RHEUMATOL VL - 35 PY - 2008 IS - 3 SP - 398 EP - 406 PG - 9 SN - 0315-162X UR - https://m2.mtmt.hu/api/publication/1144228 ID - 1144228 N1 - Drs. Kerekes and Szekanecz contributed equally to this report. AB - OBJECTIVE: Cardiovascular disease is a leading cause of mortality in rheumatoid arthritis (RA). Endothelial dysfunction often precedes manifest atherosclerosis. We assessed endothelial dysfunction and atherosclerosis in RA in context with laboratory markers. METHODS: Fifty-two patients with RA and 40 matched healthy controls were studied. We assessed common carotid intima-media thickness (ccIMT) and flow- (FMD) and nitroglycerine-mediated vasodilation (NMD). We also assayed numerous immunological and metabolic laboratory markers. RESULTS: FMD was significantly lower in RA (5.32% +/- 4.66%) compared to controls (8.30% +/- 3.96%) (p = 0.001). NMD was preserved in RA. ccIMT was significantly greater in patients with RA (0.63 +/- 0.14 mm) versus controls (0.54 +/- 0.15 mm) (p = 0.012). In patients with RA, ccIMT correlated with FMD% (R = -0.318, p = 0.022), age (R = 0.831, p < 0.001), and anti-dsDNA levels (R = 0.463, p = 0.006). FMD% correlated with serum interferon-gamma (IFN-gamma) levels (R = 0.516, p = 0.014). NMD% correlated inversely with the percentage of Th0 lymphocytes (R = -0.636, p = 0.006), serum immune complex (R = -0.692, p < 0.001), and IgM levels (R = -0.606, p = 0.003). Patients with RA were divided as "low" (< 0.65 mm) versus "high" (> 0.65 mm) ccIMT groups, and into "normal" (> 5%) versus "impaired" (< 5%) FMD% subsets. Low and high ccIMT groups differed significantly in age and serum interleukin 1 (IL-1) and anti-dsDNA levels. RA patients with normal versus impaired FMD% differed significantly in age, disease duration, and serum IFN-gamma levels. Lipoprotein(a) [Lp(a)] also correlated with rheumatoid factor (RF) and C-reactive protein (CRP); homocysteine (HCy) correlated with CRP and correlated inversely with folate and vitamin B12 production. Paraoxonase-1 (PON-1) activity correlated with serum tumor necrosis factor-alpha(TNF-alpha) and IL-6 levels. CONCLUSION: This was a well characterized RA population, where FMD and ccIMT were impaired, indicating early endothelial dysfunction and accelerated atherosclerosis, respectively. RA-related autoimmune-inflammatory mechanisms and metabolic factors including anti-CCP, RF, CRP, circulating immune complexes, IgM, TNF-alpha, IL-6, Th0/Th1 ratio, HCy, folate, vitamin B12, and PON-1 may all be involved in the development of vascular disease in RA. Although ccIMT and FMD, as well as some laboratory factors, have been assessed by other investigators in RA-associated atherosclerosis, our results regarding the possible involvement of anti-CCP, anti-dsDNA, Lp(a), some cytokines, and PON-1 activity are novel. Early determination of FMD% and ccIMT may be useful to assess RA patients with high cardiovascular risk. LA - English DB - MTMT ER - TY - JOUR AU - Pethő, Zsófia AU - Szabó, Zoltán AU - Brugós, László AU - Nemes, Zoltán AU - Szőllősi, Zoltán AU - Sándor, Zsuzsa AU - Pápay, Zsuzsanna AU - Szekanecz, Zoltán TI - Mellkasi lágyrésztumor. rheumatoid csomó? TS - rheumatoid csomó? JF - MAGYAR REUMATOLÓGIA J2 - MAGYAR REUMATOL VL - 49 PY - 2008 SP - 172 SN - 0139-4495 UR - https://m2.mtmt.hu/api/publication/1917322 ID - 1917322 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Pethő, Zsófia AU - Szabó, Zoltán AU - Brugós, László AU - Nemes, Zoltán AU - Szöllősi, Zoltán AU - Sándor, Zsuzsa AU - Pápay, Zsuzsanna AU - Szekanecz, Zoltán TI - Mellkasi lágyrésztumor: rheumatoid csomó? : esetismertetés JF - MAGYAR REUMATOLÓGIA J2 - MAGYAR REUMATOL VL - 49 PY - 2008 SP - 172 EP - 172 PG - 1 SN - 0139-4495 UR - https://m2.mtmt.hu/api/publication/31307762 ID - 31307762 N1 - 115762 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Szekanecz, Zoltán AU - Kerekes, György AU - Der, H AU - Sándor, Zsuzsa AU - Szabo, Z AU - Vegvari, A AU - Simkovics, E AU - Soos, L AU - Szentpetery, A AU - Besenyei, Tímea AU - Szűcs, Gabriella AU - Szántó, Sándor Zoltán AU - Tamasi, L AU - Szegedi, Gyula AU - Shoenfeld, Y AU - Soltész, Pál TI - Accelerated Atherosclerosis in Rheumatoid Arthritis. JF - ANNALS OF THE NEW YORK ACADEMY OF SCIENCES J2 - ANN NY ACAD SCI VL - 1108 PY - 2007 SP - 349 EP - 358 PG - 10 SN - 0077-8923 DO - 10.1196/annals.1422.036 UR - https://m2.mtmt.hu/api/publication/1091732 ID - 1091732 N1 - WoS:hiba:000249051600036 2020-09-04 03:31 típus nem egyezik AB - Cardiovascular disease is a leading cause of mortality in rheumatoid arthritis (RA). Endothelial dysfunction often precedes manifest atherosclerosis. Both traditional, Framingham risk factors and inflammation-associated factors are involved in RA-associated atherosclerosis. Among imaging techniques, the early determination of common carotid intima-media thickness (ccIMT), flow-mediated vasodilation (FMD), and nitroglycerine-mediated vasodilation (NMD) may be useful to determine atherosclerosis and endothelial dysfunction. We and others found increased ccIMT and impaired FMD in RA patients. Among immunological and metabolic laboratory markers, anticyclic citrullinated peptide (anti-CCP) antibodies, IgM rheumatoid factor, circulating immune complexes, pro-inflammatory cytokines including tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6), Th0/Th1 T cells, homocysteine, dyslipidemia, decreased folate and vitamin B12 production, and impaired paraoxonase activity may all be involved in the development of vascular disease in RA. The early diagnosis of endothelial dysfunction and atherosclerosis, active immunosuppressive treatment, the use of drugs that control atherosclerosis, changes in sedentary lifestyle, and the close follow-up of RA patients may help to minimize cardiovascular risk in these individuals. LA - English DB - MTMT ER - TY - JOUR AU - Soltész, Pál AU - Veres, Katalin AU - Szomjḱ, E AU - Kerekes, György AU - Dér, H AU - Sándor, Zsuzsa AU - Dezső, Balázs AU - Dévényi, K AU - Szekanecz, Zoltán TI - Catastrophic antiphospholipid syndrome (Asherson's syndrome) associated with cytokeratin 7-positive endometrial cancer JF - ISRAEL MEDICAL ASSOCIATION JOURNAL J2 - ISRAEL MED ASSOC J VL - 9 PY - 2007 IS - 12 SP - 891 EP - 893 PG - 3 SN - 1565-1088 UR - https://m2.mtmt.hu/api/publication/1282700 ID - 1282700 LA - English DB - MTMT ER -