TY - JOUR AU - Hudhud, Lina AU - Kovács-Rozmer, Katalin AU - Kecskés, Angéla AU - Pohóczky, Krisztina AU - Bencze, Noémi AU - Buzás, Krisztina AU - Szőke, Éva AU - Helyes, Zsuzsanna TI - Transient Receptor Potential Ankyrin 1 Ion Channel Is Expressed in Osteosarcoma and Its Activation Reduces Viability JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 7 PG - 13 SN - 1661-6596 DO - 10.3390/ijms25073760 UR - https://m2.mtmt.hu/api/publication/34797924 ID - 34797924 N1 - * Megosztott szerzőség AB - Osteosarcoma is a highly malignant, painful cancer with poor treatment opportunities and a bad prognosis. Transient receptor potential ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) receptors are non-selective cation channels that have been of great interest in cancer, as their expression is increased in some malignancies. In our study we aim to characterize the expression and functionality of the TRPA1 and TRPV1 channels in human and mouse osteosarcoma tissues and in a mouse cell line. TRPA1/Trpa1 and TRPV1/Trpv1 mRNA expressions were demonstrated by PCR gel electrophoresis and RNAscope in situ hybridization. The function of these channels was confirmed by their radioactive 45Ca2+ uptake in response to the TRPA1 agonist, Allyl-isothiocyanate (AITC), and TRPV1 agonist, capsaicin, in K7M2 cells. An ATP-based K2M7 cell viability luminescence assay was used to determine cell viability after AITC or capsaicin treatments. Both TRPA1/Trpa1 and TRPV1/Trpv1 were expressed similarly in human and mouse osteosarcoma tissues, while Trpa1 transcripts were more abundantly present in K7M2 cells. TRPA1 activation with 200 µM AITC induced a significant 45Ca2+ influx into K7M2 cells, and the antagonist attenuated this effect. In accordance with the lower Trpv1 expression, capsaicin induced a moderate 45Ca2+ uptake, which did not reach the level of statistical significance. Both AITC and capsaicin significantly reduced K7M2 cell viability, demonstrating EC50 values of 22 µM and 74 µM. The viability-decreasing effect of AITC was significantly but only partially antagonized by HC-030031, but the action of capsaicin was not affected by the TRPV1 antagonist capsazepine. We provide here the first data on the functional expression of the TRPA1 and TRPV1 ion channels in osteosarcoma, suggesting novel diagnostic and/or therapeutic perspectives. LA - English DB - MTMT ER - TY - GEN AU - Payrits, Maja AU - Csekő, Kata AU - Pohóczky, Krisztina AU - Dávid, Ernszt AU - Klaudia, Barabás AU - Nehr-Majoros, Andrea Kinga AU - Bencze, Noémi AU - Nemes, Balázs AU - Helyes, Zsuzsanna AU - Szőke, Éva TI - Effect of estradiol on the Transient Receptor Potential Vanilloid 1 and Ankyrin 1 receptors regulated pain responses PY - 2024 UR - https://m2.mtmt.hu/api/publication/34576980 ID - 34576980 LA - English DB - MTMT ER - TY - JOUR AU - Toth, Norbert AU - Hogden, Marthe Gjerstad AU - Szoke, Eva AU - Helyes, Zsuzsanna AU - Pohóczky, Krisztina TI - Expression and function of the Transient Receptor Vanilloid 1 and Ankyrin 1 ion channels in endometriosis cell line JF - BRITISH JOURNAL OF PHARMACOLOGY J2 - BR J PHARMACOL VL - 180 PY - 2023 SP - 766 EP - 767 PG - 2 SN - 0007-1188 UR - https://m2.mtmt.hu/api/publication/34212259 ID - 34212259 LA - English DB - MTMT ER - TY - JOUR AU - Kiss, Fruzsina AU - Pohóczky, Krisztina AU - Görbe, Anikó AU - Dembrovszky, Fanni AU - Kiss, Szabolcs AU - Hegyi, Péter AU - Szakó, Lajos AU - Gunkl-Tóth, Lilla AU - Somogyiné Ezer, Éva AU - Szalai, Eszter AU - Helyes, Zsuzsanna TI - Addition of epidermal growth factor receptor inhibitors to standard chemotherapy increases survival of advanced head and neck squamous cell carcinoma patients: A systematic review and meta-analysis JF - ORAL DISEASES J2 - ORAL DIS VL - 29 PY - 2023 IS - 5 SP - 1905 EP - 1919 PG - 15 SN - 1354-523X DO - 10.1111/odi.14228 UR - https://m2.mtmt.hu/api/publication/32801153 ID - 32801153 N1 - * Megosztott szerzőség AB - Head and neck squamous cell carcinoma (HNSCC) is among the common tumors associated with high mortality. The aim of our meta-analysis was to determine how additional anti-EGFR (epidermal growth factor receptor) therapy to standard chemotherapy affects the progression-free (PFS) and overall survival (OS) of the patients, besides the most common side effects. We used CENTRAL, MEDLINE and Embase databases until October 26, 2020, and included 13 eligible randomized controlled trials in our systematic research. The pooled hazard ratios (HR) for the main outcomes from the original data were estimated and for the other dichotomous outcomes, odds ratios (ORs) with their 95% confidence intervals (CI) were calculated. Addition of EGFR inhibitors to conventional chemotherapy significantly decreased the death and disease progression (for PFS HR:0.68, 95% CI:0.55-0.81, I2 =65.5%, p=0.005) and mortality (for OS HR:0.83, 95% CI:0.72-0.94, I2 =42.3%, p=0.076). In the EGFR inhibitor group, we revealed an increased chance of the over Grade 3 skin rashes (OR:4.86; 95% CI:1.52-15.49, I2 =2.3%, p=0.407), as well as all Grade skin rashes (OR:18.32, 95% CI:8.07-41.60, I2 =56.6 %, p=0.032). Despite their unwanted dermatological side effects, the addition of EGFR inhibitors are recommended to be included in advanced HNSCC therapy. LA - English DB - MTMT ER - TY - JOUR AU - Pohóczky, Krisztina AU - Kun, József AU - Szentes, Nikolett AU - Aczél, Timea AU - Urbán, Péter AU - Gyenesei, Attila AU - Bölcskei, Kata AU - Szőke, Éva AU - Sensi, Serena AU - Dénes, Ádám AU - Goebel, Andreas AU - Tékus, Valéria AU - Helyes, Zsuzsanna TI - Discovery of novel targets in a complex regional pain syndrome mouse model by transcriptomics: TNF and JAK-STAT pathways JF - PHARMACOLOGICAL RESEARCH J2 - PHARMACOL RES VL - 182 PY - 2022 PG - 15 SN - 1043-6618 DO - 10.1016/j.phrs.2022.106347 UR - https://m2.mtmt.hu/api/publication/33029393 ID - 33029393 N1 - * Megosztott szerzőség LA - English DB - MTMT ER - TY - JOUR AU - Hudhud, Lina AU - Chisholm, D.R. AU - Whiting, A. AU - Steib, Anita AU - Pohóczky, Krisztina AU - Kecskés, Angéla AU - Szőke, Éva AU - Helyes, Zsuzsanna TI - Synthetic Diphenylacetylene-Based Retinoids Induce DNA Damage in Chinese Hamster Ovary Cells without Altering Viability JF - MOLECULES J2 - MOLECULES VL - 27 PY - 2022 IS - 3 PG - 10 SN - 1420-3049 DO - 10.3390/molecules27030977 UR - https://m2.mtmt.hu/api/publication/32674506 ID - 32674506 N1 - * Megosztott szerzőség LA - English DB - MTMT ER - TY - JOUR AU - Kiss, Fruzsina AU - Kormos, Viktória AU - Szőke, Éva AU - Kecskés, Angéla AU - Tóth, Norbert AU - Steib, Anita AU - Szállási, Árpád AU - Scheich, Bálint AU - Gaszner, Balázs AU - Kun, József AU - Fülöp, Gábor AU - Pohóczky, Krisztina AU - Helyes, Zsuzsanna TI - Functional Transient Receptor Potential Ankyrin 1 and Vanilloid 1 Ion Channels Are Overexpressed in Human Oral Squamous Cell Carcinoma JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 3 PG - 14 SN - 1661-6596 DO - 10.3390/ijms23031921 UR - https://m2.mtmt.hu/api/publication/32665502 ID - 32665502 N1 - * Megosztott szerzőség AB - Oral squamous cell carcinoma (OSCC) is a common cancer with poor prognosis. Transient Receptor Potential Ankyrin 1 (TRPA1) and Vanilloid 1 (TRPV1) receptors are non-selective cation channels expressed on primary sensory neurons and epithelial and immune cells. TRPV1 mRNA and immunopositivity, as well as TRPA1-like immunoreactivity upregulation, were demonstrated in OSCC, but selectivity problems with the antibodies still raise questions and their functional relevance is unclear. Therefore, here, we investigated TRPA1 and TRPV1 expressions in OSCC and analyzed their functions. TRPA1 and TRPV1 mRNA were determined by RNAscope in situ hybridization and qPCR. Radioactive 45Ca2+ uptake and ATP-based luminescence indicating cell viability were measured in PE/CA-PJ41 cells in response to the TRPA1 agonist allyl-isothiocyanate (AITC) and TRPV1 agonist capsaicin to determine receptor function. Both TRPA1 and TRPV1 mRNA are expressed in the squamous epithelium of the human oral mucosa and in PE/CA-PJ41 cells, and their expressions are significantly upregulated in OSCC compared to healthy mucosa. TRPA1 and TRPV1 activation (100 µM AITC, 100 nM capsaicin) induced 45Ca2+-influx into PE/CA-PJ41 cells. Both AITC (10 nM–5 µM) and capsaicin (100 nM–45 µM) reduced cell viability, reaching significant decrease at 100 nM AITC and 45 µM capsaicin. We provide the first evidence for the presence of non-neuronal TRPA1 receptor in the OSCC and confirm the expression of TRPV1 channel. These channels are functionally active and might regulate cancer cell viability. LA - English DB - MTMT ER - TY - CHAP AU - Norbert, Tóth AU - Réka, Brubel AU - Kemény, Ágnes AU - Pál, Tibor AU - Polgár, Beáta AU - Bokor, Attila AU - Pohóczky, Krisztina AU - Helyes, Zsuzsanna ED - Kajos, Luca Fanni ED - Bali, Cintia ED - Preisz, Zsolt ED - Polgár, Petra Ibolya ED - Glázer-Kniesz, Adrienn ED - Tislér, Ádám ED - Szabó, Rebeka TI - Cytokine and growth factor levels positively correlate with endometriosis-related pain parameters of severe endometriosis patients T2 - 10th Jubilee Interdisciplinary Doctoral Conference : Book of Abstracts = 10. Jubileumi Interdiszciplináris Doktorandusz Konferencia : Absztraktkötet PB - Pécsi Tudományegyetem Doktorandusz Önkormányzat CY - Pécs SN - 9789634298205 PY - 2021 SP - 216 EP - 216 PG - 1 UR - https://m2.mtmt.hu/api/publication/32640881 ID - 32640881 N1 - * Megosztott szerzőség LA - English DB - MTMT ER - TY - JOUR AU - Vörös, Imre AU - Sághy, Éva AU - Pohóczky, Krisztina AU - Makkos, András AU - Onódi, Zsófia AU - Brenner, Gábor AU - Baranyai, Tamás AU - Ágg, Bence AU - Váradi, Barnabás AU - Kemény, Ágnes AU - Leszek, Przemyslaw AU - Görbe, Anikó AU - Varga, Zoltán AU - Giricz, Zoltán AU - Schulz, Rainer AU - Helyes, Zsuzsanna AU - Ferdinandy, Péter TI - Somatostatin and Its Receptors in Myocardial Ischemia/Reperfusion Injury and Cardioprotection JF - FRONTIERS IN PHARMACOLOGY J2 - FRONT PHARMACOL VL - 12 PY - 2021 PG - 15 SN - 1663-9812 DO - 10.3389/fphar.2021.663655 UR - https://m2.mtmt.hu/api/publication/32506486 ID - 32506486 N1 - * Megosztott szerzőség LA - English DB - MTMT ER - TY - JOUR AU - Dombi, Ágnes AU - Sánta, Csenge AU - Bátai, István Zoárd AU - Kormos, Viktória AU - Kecskés, Angéla AU - Tékus, Valéria AU - Pohóczky, Krisztina AU - Bölcskei, Kata AU - Pintér, Erika AU - Pozsgai, Gábor TI - Dimethyl Trisulfide Diminishes Traumatic Neuropathic Pain Acting on TRPA1 Receptors in Mice JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 22 PY - 2021 IS - 7 PG - 16 SN - 1661-6596 DO - 10.3390/ijms22073363 UR - https://m2.mtmt.hu/api/publication/31933041 ID - 31933041 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office, Hungary [OTKA FK 132454]; University of Pecs Faculty of Pharmacy [GYTK-KA-2020-01]; New National Excellence Program of the Ministry for Innovation and Technologies from the source of the National Research, Development and Innovation Fund [UNKP-20-4-II-PTE-465]; Hungarian Academy of Sciences; [EFOP-3.6.2-16-2017-00006]; [GINOP-2.3.2.-15-2016-00050]; [KTIA_NAP-20017-1-2.1.-NKP-2017-00002 20765-3/2018/FEKUTSTRAT] Funding text: OTKA FK 132454 from the National Research, Development and Innovation Office, Hungary; EFOP-3.6.2-16-2017-00006, GINOP-2.3.2.-15-2016-00050, KTIA_NAP-20017-1-2.1.-NKP-2017-00002 20765-3/2018/FEKUTSTRAT. K.P. was supported by GYTK-KA-2020-01, University of Pecs Faculty of Pharmacy and the New National Excellence Program of the Ministry for Innovation and Technologies from the source of the National Research, Development and Innovation Fund UNKP-20-4-II-PTE-465. A.K. was sponsored by Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences. AB - Pharmacotherapy of neuropathic pain is still challenging. Our earlier work indicated an analgesic effect of dimethyl trisulfide (DMTS), which was mediated by somatostatin released from nociceptor nerve endings acting on SST4 receptors. Somatostatin release occurred due to TRPA1 ion channel activation. In the present study, we investigated the effect of DMTS in neuropathic pain evoked by partial ligation of the sciatic nerve in mice. Expression of the mRNA of Trpa1 in murine dorsal-root-ganglion neurons was detected by RNAscope. Involvement of TRPA1 ion channels and SST4 receptors was tested with gene-deleted animals. Macrophage activity at the site of the nerve lesion was determined by lucigenin bioluminescence. Density and activation of microglia in the spinal cord dorsal horn was verified by immunohistochemistry and image analysis. Trpa1 mRNA is expressed in peptidergic and non-peptidergic neurons in the dorsal root ganglion. DMTS ameliorated neuropathic pain in Trpa1 and Sstr4 WT mice, but not in KO ones. DMTS had no effect on macrophage activity around the damaged nerve. Microglial density in the dorsal horn was reduced by DMTS independently from TRPA1. No effect on microglial activation was detected. DMTS might offer a novel therapeutic opportunity in the complementary treatment of neuropathic pain. LA - English DB - MTMT ER -