@article{MTMT:34797924,
	title = {Transient Receptor Potential Ankyrin 1 Ion Channel Is Expressed in Osteosarcoma and Its Activation Reduces Viability},
	url = {https://m2.mtmt.hu/api/publication/34797924},
	author = {Hudhud, Lina and Kovács-Rozmer, Katalin and Kecskés, Angéla and Pohóczky, Krisztina and Bencze, Noémi and Buzás, Krisztina and Szőke, Éva and Helyes, Zsuzsanna},
	doi = {10.3390/ijms25073760},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {25},
	unique-id = {34797924},
	issn = {1661-6596},
	abstract = {Osteosarcoma is a highly malignant, painful cancer with poor treatment opportunities and a bad prognosis. Transient receptor potential ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) receptors are non-selective cation channels that have been of great interest in cancer, as their expression is increased in some malignancies. In our study we aim to characterize the expression and functionality of the TRPA1 and TRPV1 channels in human and mouse osteosarcoma tissues and in a mouse cell line. TRPA1/Trpa1 and TRPV1/Trpv1 mRNA expressions were demonstrated by PCR gel electrophoresis and RNAscope in situ hybridization. The function of these channels was confirmed by their radioactive 45Ca2+ uptake in response to the TRPA1 agonist, Allyl-isothiocyanate (AITC), and TRPV1 agonist, capsaicin, in K7M2 cells. An ATP-based K2M7 cell viability luminescence assay was used to determine cell viability after AITC or capsaicin treatments. Both TRPA1/Trpa1 and TRPV1/Trpv1 were expressed similarly in human and mouse osteosarcoma tissues, while Trpa1 transcripts were more abundantly present in K7M2 cells. TRPA1 activation with 200 µM AITC induced a significant 45Ca2+ influx into K7M2 cells, and the antagonist attenuated this effect. In accordance with the lower Trpv1 expression, capsaicin induced a moderate 45Ca2+ uptake, which did not reach the level of statistical significance. Both AITC and capsaicin significantly reduced K7M2 cell viability, demonstrating EC50 values of 22 µM and 74 µM. The viability-decreasing effect of AITC was significantly but only partially antagonized by HC-030031, but the action of capsaicin was not affected by the TRPV1 antagonist capsazepine. We provide here the first data on the functional expression of the TRPA1 and TRPV1 ion channels in osteosarcoma, suggesting novel diagnostic and/or therapeutic perspectives.},
	keywords = {CAPSAICIN; TRPV1; Cell viability; mustard oil; TRPA1; Osteosarcoma; RNAscope in situ hybridization; radioactive 45Ca2+ uptake},
	year = {2024},
	eissn = {1422-0067},
	orcid-numbers = {Pohóczky, Krisztina/0000-0003-0385-5162; Buzás, Krisztina/0000-0001-8933-2033}
}

@misc{MTMT:34576980,
	title = {Effect of estradiol on the Transient Receptor Potential Vanilloid 1 and Ankyrin 1 receptors regulated pain responses},
	url = {https://m2.mtmt.hu/api/publication/34576980},
	author = {Payrits, Maja and Csekő, Kata and Pohóczky, Krisztina and Dávid, Ernszt and Klaudia, Barabás and Nehr-Majoros, Andrea Kinga and Bencze, Noémi and Nemes, Balázs and Helyes, Zsuzsanna and Szőke, Éva},
	unique-id = {34576980},
	year = {2024},
	orcid-numbers = {Pohóczky, Krisztina/0000-0003-0385-5162}
}

@article{MTMT:34212259,
	title = {Expression and function of the Transient Receptor Vanilloid 1 and Ankyrin 1 ion channels in endometriosis cell line},
	url = {https://m2.mtmt.hu/api/publication/34212259},
	author = {Toth, Norbert and Hogden, Marthe Gjerstad and Szoke, Eva and Helyes, Zsuzsanna and Pohóczky, Krisztina},
	journal-iso = {BR J PHARMACOL},
	journal = {BRITISH JOURNAL OF PHARMACOLOGY},
	volume = {180},
	unique-id = {34212259},
	issn = {0007-1188},
	year = {2023},
	eissn = {1476-5381},
	pages = {766-767},
	orcid-numbers = {Pohóczky, Krisztina/0000-0003-0385-5162}
}

@article{MTMT:32801153,
	title = {Addition of epidermal growth factor receptor inhibitors to standard chemotherapy increases survival of advanced head and neck squamous cell carcinoma patients: A systematic review and meta-analysis},
	url = {https://m2.mtmt.hu/api/publication/32801153},
	author = {Kiss, Fruzsina and Pohóczky, Krisztina and Görbe, Anikó and Dembrovszky, Fanni and Kiss, Szabolcs and Hegyi, Péter and Szakó, Lajos and Gunkl-Tóth, Lilla and Somogyiné Ezer, Éva and Szalai, Eszter and Helyes, Zsuzsanna},
	doi = {10.1111/odi.14228},
	journal-iso = {ORAL DIS},
	journal = {ORAL DISEASES},
	volume = {29},
	unique-id = {32801153},
	issn = {1354-523X},
	abstract = {Head and neck squamous cell carcinoma (HNSCC) is among the common tumors associated with high mortality. The aim of our meta-analysis was to determine how additional anti-EGFR (epidermal growth factor receptor) therapy to standard chemotherapy affects the progression-free (PFS) and overall survival (OS) of the patients, besides the most common side effects. We used CENTRAL, MEDLINE and Embase databases until October 26, 2020, and included 13 eligible randomized controlled trials in our systematic research. The pooled hazard ratios (HR) for the main outcomes from the original data were estimated and for the other dichotomous outcomes, odds ratios (ORs) with their 95% confidence intervals (CI) were calculated. Addition of EGFR inhibitors to conventional chemotherapy significantly decreased the death and disease progression (for PFS HR:0.68, 95% CI:0.55-0.81, I2 =65.5%, p=0.005) and mortality (for OS HR:0.83, 95% CI:0.72-0.94, I2 =42.3%, p=0.076). In the EGFR inhibitor group, we revealed an increased chance of the over Grade 3 skin rashes (OR:4.86; 95% CI:1.52-15.49, I2 =2.3%, p=0.407), as well as all Grade skin rashes (OR:18.32, 95% CI:8.07-41.60, I2 =56.6 %, p=0.032). Despite their unwanted dermatological side effects, the addition of EGFR inhibitors are recommended to be included in advanced HNSCC therapy.},
	keywords = {overall survival; EGFR INHIBITORS; head and neck squamous cell carcinoma; safety profile; PROGRESSION-FREE SURVIVAL},
	year = {2023},
	eissn = {1601-0825},
	pages = {1905-1919},
	orcid-numbers = {Kiss, Fruzsina/0000-0001-9020-1567; Pohóczky, Krisztina/0000-0003-0385-5162; Dembrovszky, Fanni/0000-0001-6953-3591; Hegyi, Péter/0000-0003-0399-7259; Szalai, Eszter/0000-0002-6723-0914}
}

@article{MTMT:33029393,
	title = {Discovery of novel targets in a complex regional pain syndrome mouse model by transcriptomics: TNF and JAK-STAT pathways},
	url = {https://m2.mtmt.hu/api/publication/33029393},
	author = {Pohóczky, Krisztina and Kun, József and Szentes, Nikolett and Aczél, Timea and Urbán, Péter and Gyenesei, Attila and Bölcskei, Kata and Szőke, Éva and Sensi, Serena and Dénes, Ádám and Goebel, Andreas and Tékus, Valéria and Helyes, Zsuzsanna},
	doi = {10.1016/j.phrs.2022.106347},
	journal-iso = {PHARMACOL RES},
	journal = {PHARMACOLOGICAL RESEARCH},
	volume = {182},
	unique-id = {33029393},
	issn = {1043-6618},
	year = {2022},
	eissn = {1096-1186},
	orcid-numbers = {Pohóczky, Krisztina/0000-0003-0385-5162}
}

@article{MTMT:32674506,
	title = {Synthetic Diphenylacetylene-Based Retinoids Induce DNA Damage in Chinese Hamster Ovary Cells without Altering Viability},
	url = {https://m2.mtmt.hu/api/publication/32674506},
	author = {Hudhud, Lina and Chisholm, D.R. and Whiting, A. and Steib, Anita and Pohóczky, Krisztina and Kecskés, Angéla and Szőke, Éva and Helyes, Zsuzsanna},
	doi = {10.3390/molecules27030977},
	journal-iso = {MOLECULES},
	journal = {MOLECULES},
	volume = {27},
	unique-id = {32674506},
	issn = {1420-3049},
	year = {2022},
	eissn = {1420-3049},
	orcid-numbers = {Pohóczky, Krisztina/0000-0003-0385-5162}
}

@article{MTMT:32665502,
	title = {Functional Transient Receptor Potential Ankyrin 1 and Vanilloid 1 Ion Channels Are Overexpressed in Human Oral Squamous Cell Carcinoma},
	url = {https://m2.mtmt.hu/api/publication/32665502},
	author = {Kiss, Fruzsina and Kormos, Viktória and Szőke, Éva and Kecskés, Angéla and Tóth, Norbert and Steib, Anita and Szállási, Árpád and Scheich, Bálint and Gaszner, Balázs and Kun, József and Fülöp, Gábor and Pohóczky, Krisztina and Helyes, Zsuzsanna},
	doi = {10.3390/ijms23031921},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {23},
	unique-id = {32665502},
	issn = {1661-6596},
	abstract = {Oral squamous cell carcinoma (OSCC) is a common cancer with poor prognosis. Transient Receptor Potential Ankyrin 1 (TRPA1) and Vanilloid 1 (TRPV1) receptors are non-selective cation channels expressed on primary sensory neurons and epithelial and immune cells. TRPV1 mRNA and immunopositivity, as well as TRPA1-like immunoreactivity upregulation, were demonstrated in OSCC, but selectivity problems with the antibodies still raise questions and their functional relevance is unclear. Therefore, here, we investigated TRPA1 and TRPV1 expressions in OSCC and analyzed their functions. TRPA1 and TRPV1 mRNA were determined by RNAscope in situ hybridization and qPCR. Radioactive 45Ca2+ uptake and ATP-based luminescence indicating cell viability were measured in PE/CA-PJ41 cells in response to the TRPA1 agonist allyl-isothiocyanate (AITC) and TRPV1 agonist capsaicin to determine receptor function. Both TRPA1 and TRPV1 mRNA are expressed in the squamous epithelium of the human oral mucosa and in PE/CA-PJ41 cells, and their expressions are significantly upregulated in OSCC compared to healthy mucosa. TRPA1 and TRPV1 activation (100 µM AITC, 100 nM capsaicin) induced 45Ca2+-influx into PE/CA-PJ41 cells. Both AITC (10 nM–5 µM) and capsaicin (100 nM–45 µM) reduced cell viability, reaching significant decrease at 100 nM AITC and 45 µM capsaicin. We provide the first evidence for the presence of non-neuronal TRPA1 receptor in the OSCC and confirm the expression of TRPV1 channel. These channels are functionally active and might regulate cancer cell viability.},
	keywords = {CAPSAICIN; TRPV1; Cell viability; TRPA1; Oral squamous cell carcinoma; AITC; RNAscope; diagnostic and prognostic biomarker; radioactive 45Ca2+ uptake; ATP-based luminescence},
	year = {2022},
	eissn = {1422-0067},
	orcid-numbers = {Kiss, Fruzsina/0000-0001-9020-1567; Gaszner, Balázs/0000-0003-2830-2732; Pohóczky, Krisztina/0000-0003-0385-5162}
}

@{MTMT:32640881,
	title = {Cytokine and growth factor levels positively correlate with endometriosis-related pain parameters of severe endometriosis patients},
	url = {https://m2.mtmt.hu/api/publication/32640881},
	author = {Norbert, Tóth and Réka, Brubel and Kemény, Ágnes and Pál, Tibor and Polgár, Beáta and Bokor, Attila and Pohóczky, Krisztina and Helyes, Zsuzsanna},
	booktitle = {10th Jubilee Interdisciplinary Doctoral Conference : Book of Abstracts = 10. Jubileumi Interdiszciplináris Doktorandusz Konferencia : Absztraktkötet},
	unique-id = {32640881},
	year = {2021},
	pages = {216-216},
	orcid-numbers = {Kemény, Ágnes/0000-0002-4523-3938; Bokor, Attila/0000-0001-5353-6990; Pohóczky, Krisztina/0000-0003-0385-5162}
}

@article{MTMT:32506486,
	title = {Somatostatin and Its Receptors in Myocardial Ischemia/Reperfusion Injury and Cardioprotection},
	url = {https://m2.mtmt.hu/api/publication/32506486},
	author = {Vörös, Imre and Sághy, Éva and Pohóczky, Krisztina and Makkos, András and Onódi, Zsófia and Brenner, Gábor and Baranyai, Tamás and Ágg, Bence and Váradi, Barnabás and Kemény, Ágnes and Leszek, Przemyslaw and Görbe, Anikó and Varga, Zoltán and Giricz, Zoltán and Schulz, Rainer and Helyes, Zsuzsanna and Ferdinandy, Péter},
	doi = {10.3389/fphar.2021.663655},
	journal-iso = {FRONT PHARMACOL},
	journal = {FRONTIERS IN PHARMACOLOGY},
	volume = {12},
	unique-id = {32506486},
	year = {2021},
	eissn = {1663-9812},
	orcid-numbers = {Vörös, Imre/0000-0001-5922-6109; Sághy, Éva/0000-0002-4031-3461; Pohóczky, Krisztina/0000-0003-0385-5162; Makkos, András/0000-0002-0309-4909; Onódi, Zsófia/0000-0002-3746-8016; Brenner, Gábor/0000-0001-7886-2960; Baranyai, Tamás/0000-0002-9378-8938; Ágg, Bence/0000-0002-6492-0426; Kemény, Ágnes/0000-0002-4523-3938; Görbe, Anikó/0000-0003-4908-1094; Varga, Zoltán/0000-0002-2758-0784; Giricz, Zoltán/0000-0003-2036-8665; Ferdinandy, Péter/0000-0002-6424-6806}
}

@article{MTMT:31933041,
	title = {Dimethyl Trisulfide Diminishes Traumatic Neuropathic Pain Acting on TRPA1 Receptors in Mice},
	url = {https://m2.mtmt.hu/api/publication/31933041},
	author = {Dombi, Ágnes and Sánta, Csenge and Bátai, István Zoárd and Kormos, Viktória and Kecskés, Angéla and Tékus, Valéria and Pohóczky, Krisztina and Bölcskei, Kata and Pintér, Erika and Pozsgai, Gábor},
	doi = {10.3390/ijms22073363},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {22},
	unique-id = {31933041},
	issn = {1661-6596},
	abstract = {Pharmacotherapy of neuropathic pain is still challenging. Our earlier work indicated an analgesic effect of dimethyl trisulfide (DMTS), which was mediated by somatostatin released from nociceptor nerve endings acting on SST4 receptors. Somatostatin release occurred due to TRPA1 ion channel activation. In the present study, we investigated the effect of DMTS in neuropathic pain evoked by partial ligation of the sciatic nerve in mice. Expression of the mRNA of Trpa1 in murine dorsal-root-ganglion neurons was detected by RNAscope. Involvement of TRPA1 ion channels and SST4 receptors was tested with gene-deleted animals. Macrophage activity at the site of the nerve lesion was determined by lucigenin bioluminescence. Density and activation of microglia in the spinal cord dorsal horn was verified by immunohistochemistry and image analysis. Trpa1 mRNA is expressed in peptidergic and non-peptidergic neurons in the dorsal root ganglion. DMTS ameliorated neuropathic pain in Trpa1 and Sstr4 WT mice, but not in KO ones. DMTS had no effect on macrophage activity around the damaged nerve. Microglial density in the dorsal horn was reduced by DMTS independently from TRPA1. No effect on microglial activation was detected. DMTS might offer a novel therapeutic opportunity in the complementary treatment of neuropathic pain.},
	keywords = {SOMATOSTATIN; Neuropathic pain; microglia; TRPA1; sciatic nerve; dimethyl trisulfide; RNAscope; SST4; partial ligation},
	year = {2021},
	eissn = {1422-0067},
	orcid-numbers = {Pohóczky, Krisztina/0000-0003-0385-5162; Pintér, Erika/0000-0001-9898-632X}
}