TY - JOUR AU - Kovács, Fruzsina AU - Balla, Noémi AU - Bozó, Aliz AU - Harmath, Andrea AU - Jakab, Ágnes AU - Tóth, Zoltán AU - Nagy, Fruzsina AU - Majoros, László AU - Kovács, Renátó TI - Epidemiology, clinical characteristics, outcome and biofilm forming properties in candidaemia: A single‐centre retrospective 4‐year analysis from Hungary JF - MYCOSES J2 - MYCOSES VL - 67 PY - 2024 IS - 4 SP - 1 SN - 0933-7407 DO - 10.1111/myc.13727 UR - https://m2.mtmt.hu/api/publication/34815621 ID - 34815621 LA - English DB - MTMT ER - TY - CONF AU - Pfliegler, Valter Péter AU - Imre, Alexandra AU - Rácz, Hanna Viktória AU - Bálint, P. Németh AU - Andrea, P. Harmath AU - Katalin, P. Pappné Murvai AU - Rizagul, P. Bazenova AU - Malika, P. Kultazina AU - Marium, P. Farooq AU - Reda, M. Lemsali AU - Antunovics, Zsuzsa AU - Kovács, Renátó AU - Pócsi, István TI - From macro- to microevolution in the yeast S. cerevisiae T2 - The Allied Genetics Conference 2024 Abstract Book PY - 2024 UR - https://m2.mtmt.hu/api/publication/34753321 ID - 34753321 LA - English DB - MTMT ER - TY - CONF AU - Imre, Alexandra AU - Kovács, Renátó AU - Ibrahim, Al’ Abri AU - Nathan, Crook AU - Pfliegler, Valter Péter TI - Specific high effect mutations in clinical and experimentally evolved Saccharomyces ‘boulardii’ isolates show that genes involved in chemical response might have a role during the adaptation to the human host T2 - The Allied Genetics Conference 2024 Abstract Book PY - 2024 UR - https://m2.mtmt.hu/api/publication/34753304 ID - 34753304 LA - English DB - MTMT ER - TY - JOUR AU - Domán, Marianna AU - Kaszab, Eszter AU - Laczkó, Levente AU - Bali, Krisztina AU - Makrai, László AU - Kovács, Renátó AU - Majoros, László AU - Bányai, Krisztián TI - Genomic epidemiology of antifungal resistance in human and avian isolates of Candida albicans: a pilot study from the One Health perspective JF - FRONTIERS IN VETERINARY SCIENCE J2 - FRONT VET SCI VL - 11 PY - 2024 SN - 2297-1769 DO - 10.3389/fvets.2024.1345877 UR - https://m2.mtmt.hu/api/publication/34609436 ID - 34609436 AB - Stress-induced genomic changes in Candida albicans contribute to the adaptation of this species to various environmental conditions. Variations of the genome composition of animal-origin C. albicans strains are largely unexplored and drug resistance or other selective pressures driving the evolution of these yeasts remained an intriguing question. Comparative genome analysis was carried out to uncover chromosomal aneuploidies and regions with loss of heterozygosity (LOH), two mechanisms that manage genome plasticity. We detected aneuploidy only in human isolates. Bird-derived isolates showed LOH in genes commonly associated with antifungal drug resistance similar to human isolates. Our study suggests that environmental fungicide usage might exert selective pressure on C. albicans infecting animals, thus contributing to the spread of potentially resistant strains between different hosts. LA - English DB - MTMT ER - TY - JOUR AU - Jakab, Ágnes AU - Csillag, Kinga Karola AU - Antal, Károly AU - Boczonádi, Imre AU - Kovács, Renátó AU - Pócsi, István AU - Emri, Tamás TI - Total transcriptome response for tyrosol exposure in Aspergillus nidulans JF - FUNGAL BIOLOGY J2 - FUNGAL BIOL-UK VL - 128 PY - 2024 IS - 2 SP - 1664 EP - 1674 PG - 11 SN - 1878-6146 DO - 10.1016/j.funbio.2024.01.003 UR - https://m2.mtmt.hu/api/publication/34524101 ID - 34524101 N1 - Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary Department of Molecular Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen, Debrecen, 4032, Hungary Department of Zoology, Faculty of Sciences, Eszterházy Károly Catholic University, Eger, 3300, Hungary HUN-REN–UD Fungal Stress Biology Research Group, Debrecen, 4032, Hungary Export Date: 18 February 2024 Correspondence Address: Jakab, Á.; Department of Medical Microbiology, Nagyerdei krt. 98., Hungary; email: jakab.agnes@med.unideb.hu Funding details: Magyar Tudományos Akadémia, MTA Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFI Funding details: National Research, Development and Innovation Office, FK138462, K131767, TKP2021-EGA-20 Funding text 1: Research was financed by the National Research, Development, and Innovation Office (Hungary) projects K131767 , and FK138462 . Project no. TKP2021-EGA-20 (Biotechnology) has been implemented with the support provided from the National Research, Development and Innovation Fund of Hungary , financed under the TKP2021-EGA funding scheme. R. K. was supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences . LA - English DB - MTMT ER - TY - JOUR AU - Jakab, Ágnes AU - Kovács, Fruzsina AU - Balla , Noémi AU - Nagy-Köteles, Csaba AU - Ragyák, Ágota AU - Nagy, Fruzsina AU - Borman, Andrew AU - Majoros, László AU - Kovács, Renátó TI - Comparative transcriptional analysis of Candida auris biofilms following farnesol and tyrosol treatment JF - MICROBIOLOGY SPECTRUM J2 - MICROBIOL SPEC PY - 2023 SN - 2165-0497 UR - https://m2.mtmt.hu/api/publication/34688574 ID - 34688574 LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Renátó AU - Majoros, László AU - Stemler, Jannik AU - Cornely, Oliver Andreas AU - Salmanton-García, Jon TI - Unveiling the Hungarian landscape of laboratory and clinical management capacities for invasive fungal infections: navigating the frontlines against fungal menaces JF - THERAPEUTIC ADVANCES IN INFECTIOUS DISEASE J2 - THERAPEUTIC ADVANCES IN INFECTIOUS DISEASE VL - 10 PY - 2023 SN - 2049-9361 DO - 10.1177/20499361231219315 UR - https://m2.mtmt.hu/api/publication/34444782 ID - 34444782 AB - Background: Antifungal diagnostic capacity has been documented in various countries, there is a lack of comprehensive research on clinical mycology diagnostics and treatment in Hungary. Methods: We conducted an online survey encompassing questions that explored various aspects of the mycology diagnostic and antifungal therapy-related information. The survey aimed to gather details about institutional profiles, perceptions of invasive fungal infections (IFIs), and access to microscopy, culture, serology, antigen detection, molecular testing, and therapeutic drug monitoring. Results: As of May 2023, a total of 17 institutions responded to the questionnaire. Seven participants categorized the institutional incidence of IFI as ‘very low’, four as ‘low’, and six as ‘mild’. The majority of centers identified Candida spp. (94%) and Aspergillus spp. (82%) as the most prevalent fungal pathogens. Nearly half of the laboratories (47%) reported using matrix-assisted laser desorption/ionization-time of flight mass spectrometry for identification. All institutions had access to microscopy and culture-based diagnostic approaches. A significant number of centers had access to antigen detection (71%) and various molecular assays (59%). Regarding antifungal agents, all reporting sites used at least one triazole, with voriconazole (77%) being the most common mold-active azole. Furthermore, 71% of the centers applied at least one formulation of amphotericin B, and 65% to one echinocandin. However, only 18% of the centers used 5-flucytosine. Conclusion: Resource availability for diagnosing and treating IFI in Hungary varies across hospitals based on location. Surveys help identify gaps and limitations in this area. To address these challenges, interregional cooperation within Hungary could be a facilitating strategy. © The Author(s), 2023. LA - English DB - MTMT ER - TY - CONF AU - Kovács, Renátó AU - Fruzsina, Kovács AU - Noémi, Balla AU - Ágota, Ragyák AU - Zsófi, Sajtos AU - László, Majoros AU - Jakab, Ágnes TI - TRANSCRIPTIONAL CHANGES AND RELATED PHYSIOLOGICAL EFFECT CAUSED BY TYROSOL EXPOSURE IN CANDIDA AURIS T2 - 16th European Conference on Fungal Genetics: Programme & Abstracts PB - Universität Innsbruck C1 - Innsbruck PY - 2023 SP - 425 UR - https://m2.mtmt.hu/api/publication/34125397 ID - 34125397 LA - English DB - MTMT ER - TY - JOUR AU - Balla , Noémi AU - Jakab, Ágnes AU - Kovács, Fruzsina AU - Ragyák, Ágota AU - Tóth, Zoltán AU - Balázsi, Dávid AU - Forgács, Lajos AU - Bozó, Aliz AU - Al Refai, Farah AU - Borman, Andrew M AU - Majoros, László AU - Kovács, Renátó TI - Total transcriptome analysis of Candida auris planktonic cells exposed to tyrosol JF - AMB EXPRESS J2 - AMB EXPRESS VL - 13 PY - 2023 IS - 1 SN - 2191-0855 DO - 10.1186/s13568-023-01586-z UR - https://m2.mtmt.hu/api/publication/34085514 ID - 34085514 N1 - Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary Doctoral School of Pharmaceutical Sciences, University of Debrecen, Debrecen, 4032, Hungary Department of Molecular Biotechnology and Microbiology, Institute of Biotechnology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary Department of Inorganic and Analytical Chemistry, Agilent Atomic Spectroscopy Partner Laboratory, University of Debrecen, Debrecen, Hungary UK National Mycology Reference Laboratory, Public Health England, Science Quarter, Southmead Hospital, Bristol, BS10 5NB, United Kingdom Medical Research Council Centre for Medical Mycology (MRCCMM), University of Exeter, Exeter, EX4 4QD, United Kingdom Export Date: 09 January 2024; Cited By: 0; Correspondence Address: R. Kovács; Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, Debrecen, Nagyerdei krt. 98, 4032, Hungary; email: kovacs.renato@med.unideb.hu AB - Tyrosol, a secondary metabolite of Candida species, regulates fungal morphogenesis, and its application may represent a novel innovative therapy against emerging multi-resistant fungal superbug such as Candida auris . In the current study, the effects of tyrosol on growth, redox homeostasis, intracellular microelement contents and activities of virulence-related enzymes released by C. auris were examined. To gain further information about the effect of tyrosol exposure, we revealed gene transcriptional changes using total transcriptome sequencing (RNA-Seq). At a concentration of 15 mM, tyrosol significantly decrease the growth of fungal cells within 2 h of its addition (5.6 × 10 7 ±1.2 × 10 7 and 2.5 × 10 7 ±0.6 × 10 7 colony forming unit/mL for control and tyrosol-treated cells, respectively). Furthermore, it enhanced the release of reactive oxygen species as confirmed by a dichlorofluorescein (DCF) assay (7.3 ± 1.8 [nmol DCF (OD 640 ) −1 ] versus 16.8 ± 3.9 [nmol DCF (OD 640 ) −1 ]), which was coincided with elevated superoxide dismutase, catalase and glutathione peroxidase activities. Tyrosol exerted in a 37%, 25%, 34% and 55% decrease in intracellular manganese, iron, zinc and copper contents, respectively, compared to control cells. The tyrosol treatment led to a 142 and 108 differentially transcripted genes with at least a 1.5-fold increase or decrease in transcription, respectively. Genes related to iron and fatty acid metabolism as well as nucleic acid synthesis were down-regulated, whereas those related to the antioxidative defence, adhesion and oxoacid metabolic processes were up-regulated. This study shows that tyrosol significantly influences growth, intracellular physiological processes and gene transcription in C. auris , which could highly support the development of novel treatment approaches against this important pathogen. LA - English DB - MTMT ER - TY - JOUR AU - Adnan, Awid AU - Borman, Andrew M. AU - Tóth, Zoltán AU - Forgács, Lajos AU - Kovács, Renátó AU - Balázsi, Dávid AU - Balázs, Bence AU - Udvarhelyi, Gergely AU - Kardos, Gábor AU - Majoros, László TI - In Vitro Killing Activities of Anidulafungin and Micafungin with and without Nikkomycin Z against Four Candida auris Clades JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 15 PY - 2023 IS - 5 SP - 1365 SN - 1999-4923 DO - 10.3390/pharmaceutics15051365 UR - https://m2.mtmt.hu/api/publication/33788072 ID - 33788072 AB - Candida auris is a multidrug-resistant pathogen against which echinocandins are the drug of choice. However, information on how the chitin synthase inhibitor nikkomycin Z influences the killing activities of echinocandins against C. auris is currently lacking. We determined the killing activities of anidulafungin and micafungin (0.25, 1, 8, 16 and 32 mg/L each) with and without nikkomycin Z (8 mg/L) against 15 isolates representing four C. auris clades (South Asian n = 5; East Asian n = 3; South African n = 3; South American n = 4, two of which were of environmental origin). Two and one isolates from the South Asian clade harbored mutations in the hot-spot 1 (S639Y and S639P) and 2 (R1354H) regions of the FKS1 gene, respectively. The anidulafungin, micafungin and nikkomycin Z MIC ranges were 0.015-4, 0.03-4 and 2->16 mg/L, respectively. Anidulafungin and micafungin alone exerted weak fungistatic activity against wild-type isolates and the isolate with a mutation in the hot-spot 2 region of FKS1 but was ineffective against the isolates with a mutation in the hot-spot 1 region. The nikkomycin Z killing curves were always similar to their respective controls. Twenty-two of sixty (36.7%) anidulafungin plus nikkomycin Z and twenty-four of sixty (40%) micafungin plus nikkomycin Z combinations produced at least 100-fold decreases in the CFUs (synergy), with a 41.7% and 20% fungicidal effect, respectively, against wild-type isolates. Antagonism was never observed. Similar results were found with the isolate with a mutation in hot-spot 2 of FKS1, but the combinations were ineffective against the two isolates with prominent mutations in hot-spot 1 of FKS1. The simultaneous inhibition of β-1,3 glucan and chitin synthases in wild-type C. auris isolates produced significantly greater killing rates than either drug alone. Further studies are warranted to verify the clinical efficacy of echinocandin plus nikkomycin Z combinations against echinocandin susceptible C. auris isolates. LA - English DB - MTMT ER -