TY - JOUR AU - Kellermayer, Dalma Lucia AU - Tordai, Hedvig AU - Kiss, Balázs AU - Török, György AU - Péter, Dániel M. AU - Sayour, Alex Ali AU - Pólos, Miklós AU - Hartyánszky, István AU - Szilveszter, Bálint AU - Labeit, Siegfried AU - Gángó, Ambrus AU - Bedics, Gábor AU - Bödör, Csaba AU - Radovits, Tamás AU - Merkely, Béla Péter AU - Kellermayer, Miklós TI - Truncated titin is structurally integrated into the human dilated cardiomyopathic sarcomere JF - JOURNAL OF CLINICAL INVESTIGATION J2 - J CLIN INVEST VL - 134 PY - 2024 IS - 2 PG - 13 SN - 0021-9738 DO - 10.1172/JCI169753 UR - https://m2.mtmt.hu/api/publication/34395421 ID - 34395421 LA - English DB - MTMT ER - TY - GEN AU - Hodrea, Judit AU - Tran, Ngoc Minh AU - Török, György AU - Ruisanchez, Éva AU - Medveczki, Tímea AU - Szabó, Attila AU - Kovács, Illés AU - Fekete, Andrea TI - A Sigma-1 Receptor aktivációja, mint új terápiás célpont a diabéteszhez társuló fibrotikus szemészeti elváltozások kezelésében PY - 2023 UR - https://m2.mtmt.hu/api/publication/33808052 ID - 33808052 N1 - {előadás} OTKA- K135398, LP2021-3/2021, TKP2021-EGA-24 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Hodrea, Judit AU - Tran, Ngoc Minh AU - Török, György AU - Ruisanchez, Éva AU - Medveczki, Tímea AU - Szabó, Attila AU - Kovács, Illés AU - Fekete, Andrea TI - A sigma-1-receptor aktivációja mint új terápiás célpont a diabéteszhez társuló fibrotikus szemészeti elváltozások kezelésében. Sigma-1 receptor activation as new approach in the treatment of diabetesassociated ocular fibrosis TS - Sigma-1 receptor activation as new approach in the treatment of diabetesassociated ocular fibrosis JF - DIABETOLOGIA HUNGARICA J2 - DIABETOLOGIA HUNGARICA VL - 31 PY - 2023 IS - Suppl. 1 SP - 26 EP - 27 PG - 2 SN - 1217-372X UR - https://m2.mtmt.hu/api/publication/33853439 ID - 33853439 LA - English DB - MTMT ER - TY - CONF AU - Seidl, Dániel AU - Antal, Violetta AU - Schay, Gusztáv AU - Aurélia, Bertholet-Thomas AU - Török, György AU - Geraldine, Mollet AU - Kellermayer, Miklós AU - Corinne, Antignac AU - Tory, Kálmán TI - Cellular basis of the dominant inheritance in NPHS2- associated glomerulopathy T2 - 31st Meeting of the European Society of Paediatric Clinical Research (ESPCR 2023) PY - 2023 SP - 34 EP - 34 PG - 1 UR - https://m2.mtmt.hu/api/publication/33871356 ID - 33871356 LA - English DB - MTMT ER - TY - JOUR AU - Tran, Ngoc Minh AU - Medveczki, Tímea AU - Besztercei, Balázs AU - Török, György AU - Szabó, Attila AU - Gasull, Xavier AU - Kovács, Illés AU - Fekete, Andrea AU - Hodrea, Judit TI - Sigma-1 Receptor Activation Is Protective against TGFβ2-Induced Extracellular Matrix Changes in Human Trabecular Meshwork Cells JF - LIFE-BASEL J2 - LIFE-BASEL VL - 13 PY - 2023 IS - 7 PG - 13 SN - 2075-1729 DO - 10.3390/life13071581 UR - https://m2.mtmt.hu/api/publication/34069454 ID - 34069454 AB - The trabecular meshwork (TM) route is the principal outflow egress of the aqueous humor. Actin cytoskeletal remodeling in the TM and extracellular matrix (ECM) deposition increase TM stiffness, outflow resistance, and elevate intraocular pressure (IOP). These alterations are strongly linked to transforming growth factor-β2 (TGFβ2), a known profibrotic cytokine that is markedly elevated in the aqueous humor of glaucomatous eyes. Sigma-1 receptor (S1R) has been shown to have neuroprotective effects in the retina, but data are lacking about its role in the TM. In this study, we identified the presence of S1R in mouse TM tissue and investigated the effect of an S1R agonist fluvoxamine (FLU) on TGFβ2-induced human TM cells regarding cell proliferation; ECM-related functions, including F-actin reorganization; and the accumulation of ECM elements. TGFβ2 increased the proliferation, cytoskeletal remodeling, and protein levels of fibronectin, collagen type IV, and connective tissue growth factor, and decreased the level of matrix metalloproteinase-2. Most importantly, FLU reversed all these effects of TGFβ2, suggesting that S1R agonists could be potential candidates for preserving TM function and thus maintaining normal IOP. LA - English DB - MTMT ER - TY - JOUR AU - Albitz, Evelin AU - Kern, Dóra AU - Kormos, Attila AU - Bojtár, Márton Gáspár AU - Török, György AU - Biró, Adrienn AU - Szatmári, Ágnes AU - Németh, Krisztina AU - Kele, Péter TI - Bioorthogonal Ligation-Activated Fluorogenic FRET Dyads JF - ANGEWANDTE CHEMIE-INTERNATIONAL EDITION J2 - ANGEW CHEM INT EDIT VL - 61 PY - 2022 IS - 6 PG - 7 SN - 1433-7851 DO - 10.1002/anie.202111855 UR - https://m2.mtmt.hu/api/publication/32552957 ID - 32552957 N1 - Funding Agency and Grant Number: Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund [NKFIH-K-131439, NKFIH-FK-137589, NKFIH-PD-135121]; New National Excellence Program of the Ministry for Innovation and Technology [UNKP-21-3]; Eotvos Lorand Research Network [KEP-6] Funding text: This work has been implemented with the support provided by the Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund, financed under the NKFIH-K-131439, NKFIH-FK-137589 and NKFIH-PD-135121 funding Schemes. We are also grateful for the generous support of Eotvos Lorand Research Network (KEP-6, FIKU). DK is grateful for the support of the uNKP-21-3 (New National Excellence Program of the Ministry for Innovation and Technology). AB - An energy transfer‐based signal amplification relay concept enabling transmission of bioorthogonally activatable fluorogenicity of blue‐excitable coumarins to yellow/red emitting cyanine frames is presented. Such relay mechanism resulted in improved cyanine fluorogenicities together with increased photostabilities and large apparent Stokes‐shifts allowing lower background fluorescence even in no‐wash bioorthogonal fluorogenic labeling schemes of intracellular structures in live cells. These energy transfer dyads sharing the same donor moiety together with their parent donor molecule allowed three‐color imaging of intracellular targets using one single excitation source with separate emission windows. Sub‐diffraction imaging of intracellular structures using the bioorthogonally activatable FRET dyads by STED microscopy is also presented. LA - English DB - MTMT ER - TY - JOUR AU - Hirmondó, Rita AU - Horváth, Ármin AU - Molnár, Dániel AU - Török, György AU - Nguyen, Liem AU - Tóth, Judit TI - The effects of mycobacterial RmlA perturbation on cellular dNTP pool, cell morphology, and replication stress in Mycobacterium smegmatis JF - PLOS ONE J2 - PLOS ONE VL - 17 PY - 2022 IS - 2 PG - 17 SN - 1932-6203 DO - 10.1371/journal.pone.0263975 UR - https://m2.mtmt.hu/api/publication/32707469 ID - 32707469 AB - The concerted action of DNA replication and cell division has been extensively investigated in eukaryotes. Well demarcated checkpoints have been identified in the cell cycle, which provides the correct DNA stoichiometry and appropriate growth in the progeny. In bacteria, which grow faster and less concerted than eukaryotes, the linkages between cell elongation and DNA synthesis are unclear. dTTP, one of the canonical nucleotide-building blocks of DNA, is also used for cell wall biosynthesis in mycobacteria. We hypothesize that the interconnection between DNA and cell wall biosynthesis through dTTP may require synchronization of these processes by regulating dTTP availability. We investigated growth, morphology, cellular dNTP pool, and possible signs of stress in Mycobacterium smegmatis upon perturbation of rhamnose biosynthesis by the overexpression of RmlA. RmlA is a cell wall synthetic enzyme that uses dTTP as the precursor for cross-linking the peptidoglycan with the arabinogalactan layers by a phosphodiester bond in the mycobacterial cell wall. We found that RmlA overexpression results in changes in cell morphology, causing cell elongation and disruption of the cylindrical cell shape. We also found that the cellular dTTP pool is reduced by half in RmlA overexpressing cells and that this reduced dTTP availability does not restrict cell growth. We observed 2-6-fold increases in the gene expression of replication and cell wall biosynthesis stress factors upon RmlA overexpression. Using super-resolution microscopy, we found that RmlA, acting to crosslink the nascent layers of the cell wall, localizes throughout the whole cell length in a helical pattern in addition to the cellular pole. LA - English DB - MTMT ER - TY - JOUR AU - Seidl, Dániel AU - Antal, Violetta AU - Schay, Gusztáv AU - Bertholet-Thomas, Aurélia AU - Török, György AU - Mollet, Geraldine AU - Kellermayer, Miklós AU - Antignac, Corinne AU - Tory, Kálmán TI - Cellular mechanism of the exceptional dominant transmission in NPHS2-associated glomerulopathy JF - NEPHROLOGY DIALYSIS TRANSPLANTATION J2 - NEPHROL DIAL TRANSPL VL - 37 PY - 2022 IS - Suppl. 3 SP - i23 EP - i23 SN - 0931-0509 DO - 10.1093/ndt/gfac062.025 UR - https://m2.mtmt.hu/api/publication/32817450 ID - 32817450 LA - English DB - MTMT ER - TY - JOUR AU - Török, György AU - Cserép, Balázs Gergely AU - Telek, András AU - Arany, Dóra AU - Váradi, Melinda AU - Homolya, László AU - Kellermayer, Miklós AU - Kele, Péter AU - Németh, Krisztina TI - Large Stokes-shift bioorthogonal probes for STED, 2P-STED and multi-color STED nanoscopy JF - METHODS AND APPLICATIONS IN FLUORESCENCE J2 - METHODS APPL FLUORESC VL - 9 PY - 2021 IS - 1 PG - 13 SN - 2050-6120 DO - 10.1088/2050-6120/abb363 UR - https://m2.mtmt.hu/api/publication/31523926 ID - 31523926 AB - Synthesis and multiple STED imaging applications of four, red-emitting (610–670 nm), tetrazine-functionalized fluorescent probes (CBRD = Chemical Biology Research group Dye 1–4) with large Stokes-shift is presented. Present studies revealed the super-resolution microscopy applicability of the probes as demonstrated through bioorthogonal labeling scheme of cytoskeletal proteins actin and keratin-19, and mitochondrial protein TOMM20. Furthermore, super-resolved images of insulin receptors in live-cell bioorthogonal labeling schemes through a genetically encoded cyclooctynylated non-canonical amino acid are also presented. The large Stokes-shifts and the wide spectral bands of the probes enabled the use of two common depletion lasers (660 nm and 775 nm). The probes were also found suitable for super-resolution microscopy in combination with two-photon excitation (2P-STED) resulting in improved spatial resolution. One of the dyes was also used together with two commercial dyes in the three-color STED imaging of intracellular structures. LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Réka AU - Vadászi, Henrietta AU - Bulyáki, Éva AU - Török, György AU - Tóth, Vilmos AU - Mátyás, Dominik AU - Kun, Judit AU - Hunyadi-Gulyás Éva, Csilla AU - Fedor, Flóra Zsófia AU - Csincsi, Ádám AU - Medzihradszky F., Katalin AU - Homolya, László AU - Juhász, Gábor Dénes AU - Kékesi, Adrienna Katalin AU - Józsi, Mihály AU - Györffy, Balázs A. AU - Kardos, József TI - Identification of Neuronal Pentraxins as Synaptic Binding Partners of C1q and the Involvement of NP1 in Synaptic Pruning in Adult Mice JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 11 PY - 2021 PG - 17 SN - 1664-3224 DO - 10.3389/fimmu.2020.599771 UR - https://m2.mtmt.hu/api/publication/31861935 ID - 31861935 N1 - ELTE NAP Neuroimmunology Research Group, Department of Biochemistry, Institute of Biology, ELTE Eötvös Loránd University, Budapest, Hungary Molecular Cell Biology Research Group, Institute of Enzymology, Research Center for Natural Sciences, Hungarian Academy of Sciences Centre of Excellence, Budapest, Hungary Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary Laboratory of Proteomics, Institute of Biology, ELTE Eötvös Loránd University, Budapest, Hungary Laboratory of Proteomics Research, Biological Research Centre, Eötvös Loránd Research Network (ELKH), Szeged, Hungary Doctoral School of Chemical Engineering and Material Sciences, Pannon University, Veszprém, Hungary Complement Research Group, Department of Immunology, ELTE Eötvös Loránd University, Budapest, Hungary Department of Physiology and Neurobiology, Institute of Biology, ELTE Eötvös Loránd University, Budapest, Hungary Cited By :4 Export Date: 23 July 2022 Correspondence Address: Kardos, J.; ELTE NAP Neuroimmunology Research Group, Hungary AB - Elements of the immune system particularly that of innate immunity, play important roles beyond their traditional tasks in host defense, including manifold roles in the nervous system. Complement-mediated synaptic pruning is essential in the developing and healthy functioning brain and becomes aberrant in neurodegenerative disorders. C1q, component of the classical complement pathway, plays a central role in tagging synapses for elimination; however, the underlying molecular mechanisms and interaction partners are mostly unknown. Neuronal pentraxins (NPs) are involved in synapse formation and plasticity, moreover, NP1 contributes to cell death and neurodegeneration under adverse conditions. Here, we investigated the potential interaction between C1q and NPs, and its role in microglial phagocytosis of synapses in adult mice. We verified in vitro that NPs interact with C1q, as well as activate the complement system. Flow cytometry, immunostaining and co-immunoprecipitation showed that synapse-bound C1q colocalizes and interacts with NPs. High-resolution confocal microscopy revealed that microglia-surrounded C1q-tagged synapses are NP1 positive. We have also observed the synaptic occurrence of C4 suggesting that activation of the classical pathway cannot be ruled out in synaptic plasticity in healthy adult animals. In summary, our results indicate that NPs play a regulatory role in the synaptic function of C1q. Whether this role can be intensified upon pathological conditions, such as in Alzheimer’s disease, is to be disclosed. LA - English DB - MTMT ER -