@article{MTMT:30677054,
	title = {A practical review on the measurement tools for cellular adhesion force},
	url = {https://m2.mtmt.hu/api/publication/30677054},
	author = {Salánki, Rita Zsanett and Péter, Beatrix and Gerecsei, Tamás and Orgován, Norbert and Horváth, Róbert and Szabó, Bálint},
	doi = {10.1016/j.cis.2019.05.005},
	journal-iso = {ADV COLLOID INTERFAC},
	journal = {ADVANCES IN COLLOID AND INTERFACE SCIENCE},
	volume = {269},
	unique-id = {30677054},
	issn = {0001-8686},
	year = {2019},
	eissn = {1873-3727},
	pages = {309-333},
	orcid-numbers = {Horváth, Róbert/0000-0001-8617-2302; Szabó, Bálint/0000-0002-9865-6336}
}

@article{MTMT:3309272,
	title = {Receptor specific adhesion assay for the quantification of integrin–ligand interactions in intact cells using a microplate based, label-free optical biosensor},
	url = {https://m2.mtmt.hu/api/publication/3309272},
	author = {Székács, Inna and Orgován, Norbert and Péter, Beatrix and Kovács, Boglárka and Horváth, Róbert},
	doi = {10.1016/j.snb.2017.09.208},
	journal-iso = {SENSOR ACTUAT B CHEM},
	journal = {SENSORS AND ACTUATORS B-CHEMICAL},
	volume = {256},
	unique-id = {3309272},
	issn = {0925-4005},
	keywords = {INTEGRINS; Cell Adhesion; Biosensor; Real-time kinetics; Ligand-receptor interaction},
	year = {2018},
	eissn = {0925-4005},
	pages = {729-734},
	orcid-numbers = {Horváth, Róbert/0000-0001-8617-2302}
}

@article{MTMT:32732704,
	title = {Antibiotic-induced release of small extracellular vesicles with surfaceassociated DNA},
	url = {https://m2.mtmt.hu/api/publication/32732704},
	author = {Németh, A and Orgován, Norbert and Sódar, Barbara and Osteikoetxea, Xabier and Pálóczi, Krisztina and Kittel, A and Turiák, Lilla and Wiener, Zoltán and Tóth, Sára and Horváth, Róbert and Buzás, Edit Irén},
	journal-iso = {J EXTRACELLULAR VESICL},
	journal = {JOURNAL OF EXTRACELLULAR VESICLES},
	volume = {7},
	unique-id = {32732704},
	year = {2018},
	eissn = {2001-3078},
	pages = {215-215},
	orcid-numbers = {Sódar, Barbara/0000-0002-8803-7304; Osteikoetxea, Xabier/0000-0003-3628-0174; Pálóczi, Krisztina/0000-0001-7065-3582; Wiener, Zoltán/0000-0001-7056-4926; Tóth, Sára/0000-0001-9852-0458; Horváth, Róbert/0000-0001-8617-2302; Buzás, Edit Irén/0000-0002-3744-206X}
}

@article{MTMT:3254659,
	title = {Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA},
	url = {https://m2.mtmt.hu/api/publication/3254659},
	author = {Németh, Andrea and Orgován, Norbert and Sódar, Barbara and Osteikoetxea, Xabier and Pálóczi, Krisztina and Szabó-Taylor, Katalin and Visnovitzné Dr Vukman, Krisztina and Kittel, Ágnes and Turiák, Lilla and Wiener, Zoltán and Tóth, Sára and Drahos, László and Vékey, Károly and Horváth, Róbert and Buzás, Edit Irén},
	doi = {10.1038/s41598-017-08392-1},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {7},
	unique-id = {3254659},
	abstract = {Recently, biological roles of extracellular vesicles (which include among others exosomes, microvesicles and apoptotic bodies) have attracted substantial attention in various fields of biomedicine. Here we investigated the impact of sustained exposure of cells to the fluoroquinolone antibiotic ciprofloxacin on the released extracellular vesicles. Ciprofloxacin is widely used in humans against bacterial infections as well as in cell cultures against <jats:italic>Mycoplasma</jats:italic> contamination. However, ciprofloxacin is an inducer of oxidative stress and mitochondrial dysfunction of mammalian cells. Unexpectedly, here we found that ciprofloxacin induced the release of both DNA (mitochondrial and chromosomal sequences) and DNA-binding proteins on the exofacial surfaces of small extracellular vesicles referred to in this paper as exosomes. Furthermore, a label-free optical biosensor analysis revealed DNA-dependent binding of exosomes to fibronectin. DNA release on the surface of exosomes was not affected any further by cellular activation or apoptosis induction. Our results reveal for the first time that prolonged low-dose ciprofloxacin exposure leads to the release of DNA associated with the external surface of exosomes.},
	year = {2017},
	eissn = {2045-2322},
	orcid-numbers = {Németh, Andrea/0000-0002-0015-8436; Sódar, Barbara/0000-0002-8803-7304; Osteikoetxea, Xabier/0000-0003-3628-0174; Pálóczi, Krisztina/0000-0001-7065-3582; Szabó-Taylor, Katalin/0000-0002-4763-3521; Wiener, Zoltán/0000-0001-7056-4926; Tóth, Sára/0000-0001-9852-0458; Drahos, László/0000-0001-9589-6652; Horváth, Róbert/0000-0001-8617-2302; Buzás, Edit Irén/0000-0002-3744-206X}
}

@article{MTMT:2991783,
	title = {Automated single cell isolation from suspension with computer vision},
	url = {https://m2.mtmt.hu/api/publication/2991783},
	author = {Salánki, Rita Zsanett and Gerecsei, Tamás and Fürjes, Péter and Orgován, Norbert and Sándor, Noémi and Holczer, Eszter Gabriella and Horváth, Róbert and Szabó, Bálint},
	doi = {10.1038/srep20375},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {6},
	unique-id = {2991783},
	abstract = {Current robots can manipulate only surface-attached cells seriously limiting the fields of their application for single cell handling. We developed a computer vision-based robot applying a motorized microscope and micropipette to recognize and gently isolate intact individual cells for subsequent analysis, e.g., DNA/RNA sequencing in 1–2 nanoliters from a thin (~100 μm) layer of cell suspension. It can retrieve rare cells, needs minimal sample preparation and can be applied for virtually any tissue cell type. Combination of 1 μm positioning precision, adaptive cell targeting and below 1 nl liquid handling precision resulted in an unprecedented accuracy and efficiency in robotic single cell isolation. Single cells were injected either into the wells of a miniature plate with a sorting speed of 3 cells/min or into standard PCR tubes with 2 cells/min. We could isolate labeled cells also from dense cultures containing ~1,000 times more unlabeled cells by the successive application of the sorting process. We compared the efficiency of our method to that of single cell entrapment in microwells and subsequent sorting with the automated micropipette: the recovery rate of single cells was greatly improved.},
	year = {2016},
	eissn = {2045-2322},
	orcid-numbers = {Fürjes, Péter/0000-0002-8022-4367; Sándor, Noémi/0000-0002-2461-1389; Horváth, Róbert/0000-0001-8617-2302; Szabó, Bálint/0000-0002-9865-6336}
}

@article{MTMT:3055425,
	title = {Intensity interrogation near cutoff resonance for label-free cellular profiling},
	url = {https://m2.mtmt.hu/api/publication/3055425},
	author = {Nazirizadeh, Yousef and Behrends, Volker and Prósz, György Aurél and Orgován, Norbert and Horváth, Róbert and M Ferrie, Ann and Fang, Ye and Selhuber-Unkel, Christine and Gerken, Martina},
	doi = {10.1038/srep24685},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {6},
	unique-id = {3055425},
	abstract = {We report a method enabling intensity-based readout for label-free cellular assays and realize a reader device with the same footprint as a microtiter plate. For unambiguous resonance intensity measurements in resonance waveguide grating (RWG) sensors, we propose to apply resonances near the substrate cutoff wavelength. This method was validated in bulk refractive index, surface bilayer and G protein-coupled receptor (GPCR) experiments. The significantly reduced size of the reader device opens new opportunities for easy integration into incubators or liquid handling systems.},
	year = {2016},
	eissn = {2045-2322},
	orcid-numbers = {Horváth, Róbert/0000-0001-8617-2302}
}

@misc{MTMT:3078629,
	title = {The modulation of human cell adhesion by genetically modified flagellin monolayers revealed by label-free optical biosensors},
	url = {https://m2.mtmt.hu/api/publication/3078629},
	author = {Kovács, Boglárka and Patkó, Dániel and Székács, Inna and Orgován, Norbert and Kurunczi, Sándor and Toth, Balazs and Vonderviszt, Ferenc and Horváth, Róbert},
	unique-id = {3078629},
	year = {2016},
	orcid-numbers = {Patkó, Dániel/0000-0002-3553-2213; Kurunczi, Sándor/0000-0002-6567-5231; Vonderviszt, Ferenc/0000-0002-6498-8172; Horváth, Róbert/0000-0001-8617-2302}
}

@misc{MTMT:3078634,
	title = {The modulation of human cell adhesion by oriented flagellin monolayers revealed by label-free optical biosensors},
	url = {https://m2.mtmt.hu/api/publication/3078634},
	author = {Kovács, Boglárka and Orgován, Norbert and Patkó, Dániel and Székács, Inna and Kurunczi, Sándor and Toth, Balazs and Vonderviszt, Ferenc and Horváth, Róbert},
	unique-id = {3078634},
	year = {2016},
	orcid-numbers = {Patkó, Dániel/0000-0002-3553-2213; Kurunczi, Sándor/0000-0002-6567-5231; Vonderviszt, Ferenc/0000-0002-6498-8172; Horváth, Róbert/0000-0001-8617-2302}
}

@article{MTMT:3095711,
	title = {Adhesion kinetics of human primary monocytes, dendritic cells, and macrophages: Dynamic cell adhesion measurements with a label-free optical biosensor and their comparison with end-point assays},
	url = {https://m2.mtmt.hu/api/publication/3095711},
	author = {Orgován, Norbert and Salánki, Rita Zsanett and Lukácsi, Szilvia Zsófia and Sándor, Noémi and Bajtay, Zsuzsanna and Erdei, Anna and Szabó, Bálint and Horváth, Róbert},
	doi = {10.1116/1.4954789},
	journal-iso = {BIOINTERPHASES},
	journal = {BIOINTERPHASES},
	volume = {11},
	unique-id = {3095711},
	issn = {1934-8630},
	abstract = {Monocytes, dendritic cells (DCs), and macrophages (MFs) are closely related immune cells that differ in their main functions. These specific functions are, to a considerable degree, determined by the differences in the adhesion behavior of the cells. To study the inherently and essentially dynamic aspects of the adhesion of monocytes, DCs, and MFs, dynamic cell adhesion assays were performed with a high-throughput label-free optical biosensor [Epic BenchTop (BT)] on surfaces coated with either fibrinogen (Fgn) or the biomimetic copolymer PLL-g-PEG-RGD. Cell adhesion profiles typically reached their maximum at ∼60 min after cell seeding, which was followed by a monotonic signal decrease, indicating gradually weakening cell adhesion. According to the biosensor response, cell types could be ordered by increasing adherence as monocytes, MFs, and DCs. Notably, all three cell types induced a larger biosensor signal on Fgn than on PLL-g-PEG-RGD. To interpret this result, the molecular layers were characterized by further exploiting the potentials of the biosensor: by measuring the adsorption signal induced during the surface coating procedure, the authors could estimate the surface density of adsorbed molecules and, thus, the number of binding sites potentially presented for the adhesion receptors. Surfaces coated with PLL-g-PEG-RGD presented less RGD sites, but was less efficient in promoting cell spreading than those coated with Fgn; hence, other binding sites in Fgn played a more decisive role in determining cell adherence. To support the cell adhesion data obtained with the biosensor, cell adherence on Fgn-coated surfaces 30–60 min after cell seeding was measured with three complementary techniques, i.e., with (1) a fluorescence-based classical adherence assay, (2) a shear flow chamber applying hydrodynamic shear stress to wash cells away, and (3) an automated micropipette using vacuum-generated fluid flow to lift cells up. These techniques confirmed the results obtained with the high-temporal-resolution Epic BT, but could only provide end-point data. In contrast, complex, nonmonotonic cell adhesion kinetics measured by the high-throughput optical biosensor is expected to open a window on the hidden background of the immune cell–extracellular matrix interactions.},
	year = {2016},
	eissn = {1559-4106},
	orcid-numbers = {Lukácsi, Szilvia Zsófia/0000-0003-3499-8422; Sándor, Noémi/0000-0002-2461-1389; Bajtay, Zsuzsanna/0000-0002-0705-0321; Erdei, Anna/0000-0002-3622-6680; Szabó, Bálint/0000-0002-9865-6336; Horváth, Róbert/0000-0001-8617-2302}
}

@article{MTMT:3105834,
	title = {Flagellin based biomimetic coatings: From cell-repellent surfaces to highly adhesive coatings},
	url = {https://m2.mtmt.hu/api/publication/3105834},
	author = {Kovács, Boglárka and Patkó, Dániel and Székács, Inna and Orgován, Norbert and Kurunczi, Sándor and Sulyok, Attila and Nguyen Quoc, Khánh and Toth, B and Vonderviszt, Ferenc and Horváth, Róbert},
	doi = {10.1016/j.actbio.2016.07.002},
	journal-iso = {ACTA BIOMATER},
	journal = {ACTA BIOMATERIALIA},
	volume = {42},
	unique-id = {3105834},
	issn = {1742-7061},
	abstract = {Biomimetic coatings with cell-adhesion-regulating functionalities are intensively researched today. For example, cell-based biosensing for drug development, biomedical implants, and tissue engineering require that the surface adhesion of living cells is well controlled. Recently, we have shown that the bacterial flagellar protein, flagellin, adsorbs through its terminal segments to hydrophobic surfaces, forming an oriented monolayer and exposing its variable D3 domain to the solution. Here, we hypothesized that this nanostructured layer is highly cell-repellent since it mimics the surface of the flagellar filaments. Moreover, we proposed flagellin as a carrier molecule to display the cell-adhesive RGD (Arg-Gly-Asp) peptide sequence and induce cell adhesion on the coated surface. The D3 domain of flagellin was replaced with one or more RGD motifs linked by various oligopeptides modulating flexibility and accessibility of the inserted segment. The obtained flagellin variants were applied to create surface coatings inducing cell adhesion and spreading to different levels, while wild-type flagellin was shown to form a surface layer with strong anti-adhesive properties. As reference surfaces synthetic polymers were applied which have anti-adhesive (PLL-g-PEG poly(L-lysine)-graft-poly(ethylene glycol)) or adhesion inducing properties (RGD-functionalized PLL-g-PEG). Quantitative adhesion data was obtained by employing optical biochips and microscopy. Cell-adhesion-regulating coatings can be simply formed on hydrophobic surfaces by using the developed flagellin-based constructs. The developed novel RGD-displaying flagellin variants can be easily obtained by bacterial production and can serve as alternatives to create cell-adhesion-regulating biomimetic coatings. Statement of Significance In the present work, we show for the first time that - an oriented and dense monolayer of flagellin molecules mimics the surface of the bacterial flagellar filament. Consequently, the fabricated layer is completely cell repellent.- By genetically modifying flagellin, we incorporate cell adhesion regulating functionalities into this anti-adhesive coating.- We can easily tune the adhesion of living cells from completely cell repellent to highly adhesive. © 2016 Acta Materialia Inc.},
	keywords = {Cell Adhesion; FLAGELLIN; Tunable surface coating; RGD-displaying flagellin; RGD motif; Hydrophobic surface modification; Biomimetic film},
	year = {2016},
	eissn = {1878-7568},
	pages = {66-76},
	orcid-numbers = {Patkó, Dániel/0000-0002-3553-2213; Kurunczi, Sándor/0000-0002-6567-5231; Nguyen Quoc, Khánh/0000-0001-7032-2919; Vonderviszt, Ferenc/0000-0002-6498-8172; Horváth, Róbert/0000-0001-8617-2302}
}