TY - JOUR AU - Várnagy, Ákos AU - Mauchart, Péter AU - Nagy, Gábor AU - Bódis, József AU - Sulyok, Endre TI - Follicular Fluid Vanin-1 Levels in Patients Undergoing Ivf: A Preliminary Study JF - ANTIOXIDANTS J2 - ANTIOXIDANTS-BASEL VL - 14 PY - 2025 IS - 2 PG - 9 SN - 2076-3921 DO - 10.3390/antiox14020133 UR - https://m2.mtmt.hu/api/publication/35752662 ID - 35752662 N1 - Funding Agency and Grant Number: Recovery and Resilience Facility of the European Union within the framework of Programme Szechenyi Plan Plus [RRF-2.3.1-21-2022-00012]; HUN-REN Hungarian Research Network Funding text: Project no. RRF-2.3.1-21-2022-00012, titled National Laboratory on Human Reproduction, received support provided by the Recovery and Resilience Facility of the European Union within the framework of Programme Szechenyi Plan Plus. This project received funding from the HUN-REN Hungarian Research Network. AB - This preliminary study was designed to determine follicular fluid (FF) vanin-1 levels, to assess their relation to serum vanin-1 and to reveal their potential to predict the outcome of in vitro fertilization (IVF). Eighteen unselected, consecutive women undergoing IVF were included. Serum and pooled FF samples were obtained simultaneously during routine IVF procedures. Vanin-1 levels were measured by using commercially available ELISA kits. As most of the values were below 0.6 ng/mL, the data are given as optical density. It was found that vanin-1 can be detected in FF and that it is not significantly related to its maternal serum levels (p = 0.06). FF vanin-1 levels proved to be higher in non-pregnant as compared to pregnant women (p < 0.04). There are significant positive relationships between the FF to serum vanin-1 ratio and body mass index (BMI, p < 0.02), anti-Müllerian hormone (AMH, p < 0.02) and baseline serum estradiol (p < 0.01). Moreover, the FF/serum vanin-1 ratio tended to increase with cumulative FSH dose, but this increase did not reach statistical significance (p = 0.064). It may be concluded that FF vanin-1 may serve as a biomarker to predict IVF outcome. To confirm this contention, further studies are to be performed. LA - English DB - MTMT ER - TY - JOUR AU - Bódis, József AU - Nagy, Bernadett AU - Bognár, Zoltán AU - Csabai-Tanics, Tímea Judith AU - Berke, József AU - Gulyás, István AU - Mauchart, Péter AU - H. R, Tinneberg AU - Farkas, Bálint AU - Várnagy, Ákos AU - Kovács, Kálmán András TI - Detection of Ultra-Weak Photon Emissions from Mouse Embryos with Implications for Assisted Reproduction JF - Journal of Healthcare Communications J2 - Journal of Healthcare Communications VL - 9 PY - 2024 IS - 4 PG - 6 SN - 2472-1654 UR - https://m2.mtmt.hu/api/publication/35627338 ID - 35627338 LA - English DB - MTMT ER - TY - JOUR AU - Berke, József AU - Gulyás, István AU - Bognár, Zoltán AU - Berke, Dávid AU - Enyedi, Attila AU - Kozma-Bognár, Veronika AU - Mauchart, Péter AU - Nagy, Bernadett AU - Várnagy, Ákos AU - Kovács, Kálmán András AU - Bódis, József TI - Unique algorithm for the evaluation of embryo photon emission and viability. JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 PG - 14 SN - 2045-2322 DO - 10.1038/s41598-024-61100-8 UR - https://m2.mtmt.hu/api/publication/35133462 ID - 35133462 AB - Living cells have spontaneous ultraweak photon emission derived from metabolic reactions associated with physiological conditions. The ORCA-Quest CMOS camera (Hamamatsu Photonics, Japan) is a highly sensitive and essential tool for photon detection; its use with a microscope incubator (Olympus) enables the detection of photons emitted by embryos with the exclusion of harmful visible light. With the application of the second law of thermodynamics, the low-entropy energy absorbed and used by embryos can be distinguished from the higher-entropy energy released and detectable in their environment. To evaluate higher-entropy energy data from embryos, we developed a unique algorithm for the calculation of the entropy-weighted spectral fractal dimension, which demonstrates the self-similar structure of the energy (photons) released by embryos. Analyses based on this structure enabled the distinction of living and degenerated mouse embryos, and of frozen and fresh embryos and the background. This novel detection of ultra-weak photon emission from mouse embryos can provide the basis for the development of a photon emission embryo control system. The ultraweak photon emission fingerprints of embryos may be used for the selection of viable specimens in an ideal dark environment. LA - English DB - MTMT ER - TY - JOUR AU - Gödöny, Krisztina AU - Herczeg, Róbert AU - Gyenesei, Attila AU - Várnagy, Ákos AU - Bognár, Zoltán AU - Kovács, L. Gábor AU - Szekeres, Júlia AU - Mauchart, Péter AU - Nagy, Bernadett AU - Erostyák, János AU - Kovács, Kálmán András AU - Bódis, József TI - Clinical Benefits of Decreased Photo-Oxidative Stress on Human Embryo Development JF - MEDICAL PRINCIPLES AND PRACTICE J2 - MED PRIN PRACT VL - 33 PY - 2024 IS - 2 SP - 112 EP - 121 PG - 10 SN - 1011-7571 DO - 10.1159/000536358 UR - https://m2.mtmt.hu/api/publication/34763514 ID - 34763514 AB - Objective: Early embryonic development is characterized by rapid cell division and gene activation, making the embryo extremely sensitive to environmental influences. Light exposure can affect embryonic development through a direct toxic effect on the embryo via the generation of reactive oxygen species. In a previous study, we demonstrated the positive effect of improved light-protected embryo culture conditions implemented in our laboratory. This study aimed to investigate the changes in human embryo development under light protection during the conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Materials and Methods: We tested the potential beneficial effect of light filters to reduce the risk of toxic effects of light. IVF outcomes were compared between two experimental conditions, light protection with red light filters versus no light protection as a control. Results: Blastocyst development rate in IVF was significantly higher in the light-protected group than in the group treated under conventional conditions (46.6 vs. 26.7%). In the case of ICSI, we obtained a similar result (44.5 vs. 31.6%). The rate of cryopreservation with at least one embryo was higher in the light-protected phase (32.8%) than in the conventionally manipulated phase (26.8%). The abortion rate was also significantly lower during the light-protected period in IVF, resulting in a higher live birth rate. Conclusions: The implementation of light protection to reduce the embryotoxic wavelengths of light in IVF centers may improve the blastocyst development rate and embryo quality while maintaining embryo safety. LA - English DB - MTMT ER - TY - GEN AU - Bódis, József AU - Bognár, Zoltán AU - Csabai-Tanics, Tímea Judith AU - Berke, József AU - Gulyás, István Tibor AU - Mauchart, Péter AU - Hans-Rudolf, Tinneberg AU - Farkas, Bálint AU - Várnagy, Ákos AU - Kovács, Kálmán András TI - Detection of Ultraweak Photon Emissions from Mouse Embryos with Implications for Assisted Reproduction PY - 2024 UR - https://m2.mtmt.hu/api/publication/34525481 ID - 34525481 LA - English DB - MTMT ER - TY - GEN AU - Berke, József AU - Gulyás, István Tibor AU - Bognár, Zoltán AU - Dávid, Berke AU - Attila, Enyedi AU - Veronika, Kozma-Bognár AU - Mauchart, Péter AU - Nagy, Bernadett AU - Várnagy, Ákos AU - Kovács, Kálmán András AU - Bódis, József TI - Unique algorithm for the evaluation of embryo photon emission and viability PY - 2024 UR - https://m2.mtmt.hu/api/publication/34525317 ID - 34525317 N1 - National Laboratory on Human Reproduction, University of Pécs, Pécs, Hungary Department of Drone Technology and Image Processing, Dennis Gabor University, Budapest, Hungary Department of Medical Biology and Central Electron Microscope Laboratory, Medical School, University of Pecs, Pécs, Hungary HUN-REN–PTE Human Reproduction Research Group, Pécs, Hungary John Von Neumann Computer Society, Multimedia in Education Section, Budapest, Hungary Dennis Gabor University, Rector’s Cabinet, Budapest, Hungary Department of Obstetrics and Gynecology, Medical School, University of Pecs, Pécs, Hungary Export Date: 26 October 2024 LA - English DB - MTMT ER - TY - JOUR AU - Kurdi, Csilla AU - Schmidt, János AU - Horváth-Szalai, Zoltán AU - Mauchart, Péter AU - Gödöny, Krisztina AU - Várnagy, Ákos AU - Kovács, L. Gábor AU - Kőszegi, Tamás TI - Follicular Fluid Proteomic Analysis of Women Undergoing Assisted Reproduction Suggests That Apolipoprotein A1 Is a Potential Fertility Marker JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 1 PG - 15 SN - 1661-6596 DO - 10.3390/ijms25010486 UR - https://m2.mtmt.hu/api/publication/34486045 ID - 34486045 AB - Infertility affects millions worldwide, posing a significant global health challenge. The proteomic analysis of follicular fluid provides a comprehensive view of the complex molecular landscape within ovarian follicles, offering valuable information on the factors influencing oocyte development and on the overall reproductive health. The follicular fluid is derived from the plasma and contains various proteins that can have different roles in oocyte health and infertility, and this fluid is a critical microenvironment for the developing oocytes as well. Using the high-performance liquid chromatography-mass spectrometry method, we investigated the protein composition of the follicular fluid, and after classification, we carried out relative quantification of the identified proteins in the pregnant (P) and non-pregnant (NP) groups. Based on the protein–protein interaction analysis, albumin and apolipoprotein A1 (ApoA1) were found to be hub proteins, and the quantitative comparison of the P and NP groups resulted in a significantly lower concentration of ApoA1 and high-density lipoprotein cholesterol in the P group. As both molecules are involved in the cholesterol transport, we also investigated their role in the development of oocytes and in the prediction of fertility. LA - English DB - MTMT ER - TY - JOUR AU - Kurdi, Csilla AU - Sipos-Lelovics, Vanessza AU - Hesszenberger, Dávid AU - Lajtai, Anikó AU - Lakatos, Ágnes AU - Herczeg, Róbert AU - Gödöny, Krisztina AU - Mauchart, Péter AU - Várnagy, Ákos AU - Kovács, L. Gábor AU - Kőszegi, Tamás TI - Amino Acid Profiling of Follicular Fluid in Assisted Reproduction Reveals Important Roles of Several Amino Acids in Patients with Insulin Resistance JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 15 PG - 15 SN - 1661-6596 DO - 10.3390/ijms241512458 UR - https://m2.mtmt.hu/api/publication/34106678 ID - 34106678 AB - The global prevalence of insulin resistance (IR) is increasing continuously, influencing metabolic parameters and fertility. The metabolic changes due to IR can alter the molecular composition of plasma and other body fluids. Follicular fluid (FF) is derived mainly from plasma, and it is a critical microenvironment for the developing oocytes. It contains various metabolites and amino acids, and the quality of the oocytes is linked at least partially to amino acid metabolism. Our goal was to quantitatively determine the amino acid (AA) profile of FF in IVF patients and to compare IR and non-insulin resistance (NIR) groups to investigate the AA changes in their FF. Using UHPLC-based methods, we quantified the main 20 amino acids from human FF samples in the IR and NIR groups. Several amino acids (aspartate, glycine, glutamate, and cysteine) differed significantly (p < 0.05 or less) between the two groups. The most significant alterations between the IR and NIR groups were related to the glutathione metabolic pathway involving glycine, serine, and threonine. Since insulin resistance alters the amino acid composition of the FF, the oocytes may undergo metabolism-induced changes resulting in poor oocyte quality and less fertility in the insulin resistance groups. LA - English DB - MTMT ER - TY - JOUR AU - Mauchart, Péter AU - Vass, Réka Anna AU - Nagy, Bernadett AU - Sulyok, Endre AU - Bódis, József AU - Kovács, Kálmán András TI - Oxidative Stress in Assisted Reproductive Techniques, with a Focus on an Underestimated Risk Factor. JF - CURRENT ISSUES IN MOLECULAR BIOLOGY J2 - CURR ISSUES MOL BIOL VL - 45 PY - 2023 IS - 2 SP - 1272 EP - 1286 PG - 15 SN - 1467-3037 DO - 10.3390/cimb45020083 UR - https://m2.mtmt.hu/api/publication/33667137 ID - 33667137 AB - Based on current findings, the presence of oxidative stress has a significant impact on the quality of gametes and embryos when performing assisted reproductive techniques (ART). Unfortunately, in vitro manipulation of these cells exposes them to a higher level of reactive oxygen species (ROS). The primary goal of this review is to provide a comprehensive overview of the development of oxidative stress in female and male reproductive systems, as well as in the case of the pre-implantation embryo and its environment. This review also focuses on the origins of ROS and the mechanisms of oxidative stress-induced damage during ART procedures. A well-known but underestimated hazard, light exposure-related photo-oxidation, is particularly concerning. The effect of oxidative stress on ART outcomes, as well as the various strategies for preventing it, are also discussed. We emphasize the role and significance of antioxidants and light protection including forms, functions, and mechanisms in the development of gametes and embryos in vivo and in vitro. LA - English DB - MTMT ER - TY - JOUR AU - Péntek, Sándor Mátyás AU - Várnagy, Ákos AU - Farkas, Bálint AU - Mauchart, Péter AU - Gödöny, Krisztina AU - Varjas, Tímea AU - Kőszegi, Tamás AU - Kaltenecker, Péter AU - Jakabfi-Csepregi, Rita AU - Kovács, Kálmán András AU - Bódis, József AU - Sulyok, Endre TI - Telomere Length and Telomerase Activity of Granulosa Cells and Follicular Fluid in Women Undergoing In Vitro Fertilization. JF - ANTIOXIDANTS J2 - ANTIOXIDANTS-BASEL VL - 12 PY - 2023 IS - 2 PG - 13 SN - 2076-3921 DO - 10.3390/antiox12020419 UR - https://m2.mtmt.hu/api/publication/33667134 ID - 33667134 AB - This study aimed to evaluate the interrelationship between telomere length, telomerase activity and oxidative DNA damage in patients undergoing in vitro fertilization (IVF). This single-center, observational clinical study comprised 102 unselected, consecutive patients with various infertility diagnoses. Granulosa cells (GCs) and follicular fluid (FF) were analyzed simultaneously for telomere functions and for the marker of oxidative DNA damage, 8-hydroxy-2-deoxyguanosine (8-OHdG). An Absolute Human Telomere Lengths Quantification qPCR Assay kit and Telomerase Activity Quantification qPCR Assay kit (Nucleotestbio, Budapest, Hungary), as well as an 8-OHdG ELISA kit (Abbexa Ltd., Cambridge, United Kingdom) were used for analyses. Similar telomere lengths were found in GCs and FF, however telomerase activity was markedly depressed, while 8-OHdG levels were markedly elevated in FF compared with those in GCs (p < 0.01). Telomere lengths were independent of telomerase activity both in GCs and FF. However, GC 8-OHdG was inversely related to telomerase activity in GCs and FF (p < 0.05). Importantly, 8-OHdG levels both in GCs and FF had significant negative impact on the number of the retrieved and MII oocytes (p < 0.01), whereas FF 8-OHdG was negatively related further to the number of fertilized oocytes and blastocysts (p < 0.01). In conclusion, we could not confirm the direct association of telomere function and reproductive potential. However, oxidative DNA damage, as mainly reflected by 8-OHdG, adversely affected early markers of IVF outcome and clinical pregnancies. LA - English DB - MTMT ER -