TY - JOUR AU - Ábrahám, Edit AU - Bajusz, Csaba AU - Marton, Annamária AU - Borics, Attila AU - Mdluli, Thandiswa AU - Pardi, Norbert AU - Lipinszki, Zoltán TI - Expression and purification of the receptor-binding domain of SARS-CoV-2 spike protein in mammalian cells for immunological assays JF - FEBS OPEN BIO J2 - FEBS OPEN BIO VL - 14 PY - 2024 IS - 3 SP - 380 EP - 389 PG - 10 SN - 2211-5463 DO - 10.1002/2211-5463.13754 UR - https://m2.mtmt.hu/api/publication/34575640 ID - 34575640 N1 - Funding Agency and Grant Number: National Laboratory for Biotechnology [2022-2.1.1-NL-2022-00008]; Hungarian Academy of Sciences (Lenduelet Program Grant) [LP2017-7/2017]; National Research, Development and Innovation Office [K143124]; National Institute of Allergy and Infectious Diseases [R01AI146101, R01AI153064] Funding text: This work was supported by the National Laboratory for Biotechnology (2022-2.1.1-NL-2022-00008) to CB and ZL, the Hungarian Academy of Sciences (Lenduelet Program Grant (LP2017-7/2017)) to ZL, and the National Research, Development and Innovation Office (K143124) to AB. NP was supported by the National Institute of Allergy and Infectious Diseases (R01AI146101 and R01AI153064). The authors are grateful for Adam Pap and Zsuzsanna Darula (BRC) for the PNGase F enzyme, and Petar Lambrev (BRC) for the Jasco J-815 CD spectropolarimeter. AB - The receptor-binding domain (RBD) of the spike glycoprotein of SARS-CoV-2 virus mediates the interaction with the host cell and is required for virus internalization. It is, therefore, the primary target of neutralizing antibodies. The receptor-binding domain soon became the major target for COVID-19 research and the development of diagnostic tools and new-generation vaccines. Here, we provide a detailed protocol for high-yield expression and one-step affinity purification of recombinant RBD from transiently transfected Expi293F cells. Expi293F mammalian cells can be grown to extremely high densities in a specially formulated serum-free medium in suspension cultures, which makes them an excellent tool for secreted protein production. The highly purified RBD is glycosylated, structurally intact, and forms homomeric complexes. With this quick and easy method, we are able to produce large quantities of RBD (80 mg center dot L-1 culture) that we have successfully used in immunological assays to examine antibody titers and seroconversion after mRNA-based vaccination of mice. LA - English DB - MTMT ER - TY - JOUR AU - Varga-Zsíros, Vanda AU - Migh, Ede AU - Marton, Annamária AU - Kóta, Zoltán AU - Vizler, Csaba AU - Tiszlavicz, László AU - Horváth, Péter AU - Török, Zsolt AU - Vigh, László AU - Balogh, Gábor AU - Péter, Mária TI - Development of a Laser Microdissection-Coupled Quantitative Shotgun Lipidomic Method to Uncover Spatial Heterogeneity JF - CELLS J2 - CELLS-BASEL VL - 12 PY - 2023 IS - 3 PG - 16 SN - 2073-4409 DO - 10.3390/cells12030428 UR - https://m2.mtmt.hu/api/publication/33607647 ID - 33607647 AB - Lipid metabolic disturbances are associated with several diseases, such as type 2 diabetes or malignancy. In the last two decades, high-performance mass spectrometry-based lipidomics has emerged as a valuable tool in various fields of biology. However, the evaluation of macroscopic tissue homogenates leaves often undiscovered the differences arising from micron-scale heterogeneity. Therefore, in this work, we developed a novel laser microdissection-coupled shotgun lipidomic platform, which combines quantitative and broad-range lipidome analysis with reasonable spatial resolution. The multistep approach involves the preparation of successive cryosections from tissue samples, cross-referencing of native and stained images, laser microdissection of regions of interest, in situ lipid extraction, and quantitative shotgun lipidomics. We used mouse liver and kidney as well as a 2D cell culture model to validate the novel workflow in terms of extraction efficiency, reproducibility, and linearity of quantification. We established that the limit of dissectible sample area corresponds to about ten cells while maintaining good lipidome coverage. We demonstrate the performance of the method in recognizing tissue heterogeneity on the example of a mouse hippocampus. By providing topological mapping of lipid metabolism, the novel platform might help to uncover region-specific lipidomic alterations in complex samples, including tumors. LA - English DB - MTMT ER - TY - JOUR AU - Gál, László AU - Bellák, Tamás AU - Marton, Annamária AU - Fekécs, Zoltán AU - Weissman, Drew AU - Török, Dénes AU - Biju, Rachana AU - Vizler, Csaba AU - Kristóf, Rebeka AU - Beattie, Mitchell B. AU - Lin, Paulo J.C. AU - Pardi, Norbert AU - Nógrádi, Antal AU - Pajer, Krisztián TI - Restoration of Motor Function through Delayed Intraspinal Delivery of Human IL-10-Encoding Nucleoside-Modified mRNA after Spinal Cord Injury JF - RESEARCH J2 - RESEARCH-CHINA VL - 6 PY - 2023 PG - 14 SN - 2096-5168 DO - 10.34133/research.0056 UR - https://m2.mtmt.hu/api/publication/33575768 ID - 33575768 N1 - Department of Anatomy, Histology and Embryology, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary National Biotechnology Laboratory, Institute of Genetics, Biological Research Centre, Szeged, Hungary Institute of Biochemistry, Biological Research Centre, Szeged, Hungary Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, United States Acuitas Therapeutics, Vancouver, BC V6T 1Z3, Canada Export Date: 27 April 2023 Correspondence Address: Nógrádi, A.; Department of Anatomy, Hungary; email: nogradi.antal@med.u-szeged.hu Funding details: National Institutes of Health, NIH, 2020-1.1.6-JÖVŐ-2021-00012, 2022-2.1.1-NL-2022-00008, R01-AI153064 Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFIH, KLINO-117031 Funding text 1: We thank S. B. Sangeetham for the professional assistance (Department of Anatomy, Histology and Embryology, Szent-Györgyi Albert Medical School, University of Szeged). Funding: N.P. was supported by NIH R01-AI153064. C.V. and A.M. received generous support from the following grants: 2020-1.1.6-JÖVŐ-2021-00012 and 2022-2.1.1-NL-2022-00008 for the National Biotechnology Laboratory. A.N. was supported by the NKFIH KLINO-117031 grant. Author contributions: A.N., K.P., and L.G. designed the experiments. L.G., T.B., K.P., Z.F., and D.T. performed the surgical and gait analysis experiments. L.G. and R.B. collected the samples and managed all tissue processing, staining, and imaging. A.M. and C.V. performed and analyzed the proteome profiler and ELISA. M.B.B. and P.J.C.L. prepared the LNPs. R.K. performed the PCR experiments and data analysis. L.G., T.B., K.P., and Z.F. prepared the figures. A.N., K.P., and N.P. wrote the manuscript. N.P., D.W., M.B.B., P.J.C.L., and A.N. revised the manuscript. All authors discussed and helped prepare the manuscript. Competing interests: The authors declare that they have no competing interests. LA - English DB - MTMT ER - TY - CONF AU - Gál, László AU - Bellák, Tamás AU - Marton, Annamária AU - Fekécs, Zoltán AU - Drew, Weissman AU - Török, Dénes AU - Rachana, Biju AU - Csaba, Vizler AU - Paulo, J.C. Lin AU - Ying, K. Tam AU - Pardi, Norbert AU - Nógrádi, Antal AU - Pajer, Krisztián TI - Transcribed messenger RNA – a potential therapeutic platform for spinal cord injury T2 - International Neuroscience Meeting, Budapest 2022 PY - 2022 SP - 46 EP - 46 PG - 1 UR - https://m2.mtmt.hu/api/publication/32638954 ID - 32638954 LA - English DB - MTMT ER - TY - CONF AU - Bellák, Tamás AU - Pajer, Krisztián AU - Fekécs, Zoltán AU - Ignácz, Máté AU - Marton, Annamária AU - Gál, László AU - Török, Dénes AU - Csaba, Vizler AU - Nógrádi, Antal TI - Application of neuroectodermal stem cells supports functional and morphological recovery after chronic spinal cord contusion injury T2 - International Neuroscience Meeting, Budapest 2022 PY - 2022 SP - 41 EP - 41 PG - 1 UR - https://m2.mtmt.hu/api/publication/32638588 ID - 32638588 LA - English DB - MTMT ER - TY - JOUR AU - Pető, Ágota AU - Kósa, Dóra AU - Haimhoffer, Ádám AU - Siposné Fehér, Pálma AU - Ujhelyi, Zoltán AU - Sinka, Dávid AU - Fenyvesi, Ferenc AU - Váradi, Judit AU - Vecsernyés, Miklós AU - Gyöngyösi, Alexandra AU - Lekli, István AU - Szentesi, Péter AU - Marton, Annamária AU - Gombos, Imre AU - Dukic, Barbara AU - Vigh, László AU - Bácskay, Ildikó TI - Nicotinic Amidoxime Derivate BGP-15, Topical Dosage Formulation and Anti-Inflammatory Effect JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 13 PY - 2021 IS - 12 PG - 17 SN - 1999-4923 DO - 10.3390/pharmaceutics13122037 UR - https://m2.mtmt.hu/api/publication/32515212 ID - 32515212 N1 - Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei Körút 98, Debrecen, H-4032, Hungary Doctoral School of Pharmaceutical Sciences, University of Debrecen, Nagyerdei Körút 98, Debrecen, H-4032, Hungary Institute of Healthcare Industry, University of Debrecen, Nagyerdei Körút 98, Debrecen, H-4032, Hungary Department of Pharmacology, Faculty of Pharmacy, University of Debrecen, Nagyerdei Körút 98, Debrecen, H-4032, Hungary Department of Physiology, Faculty of Medicine, University of Debrecen, Nagyerdei Körút 98, Debrecen, H-4032, Hungary Institute of Biochemistry, Biological Research Center, Temesvári Körút 62, Szeged, H-6726, Hungary Cited By :5 Export Date: 3 May 2024 Correspondence Address: Bácskay, I.; Department of Pharmaceutical Technology, Nagyerdei Körút 98, Hungary; email: bacskay.ildiko@pharm.unideb.hu AB - BGP-15 is a Hungarian-developed drug candidate with numerous beneficial effects. Its potential anti-inflammatory effect is a common assumption, but it has not been investigated in topical formulations yet. The aim of our study was to formulate 10% BGP-15 creams with different penetration enhancers to ensure good drug delivery, improve bioavailability of the drug and investigate the potential anti-inflammatory effect of BGP-15 creams in vivo. Since the exact mechanism of the effect is still unknown, the antioxidant effect (tested with UVB radiation) and the ability of BGP-15 to decrease macrophage activation were evaluated. Biocompatibility investigations were carried out on HaCaT cells to make sure that the formulations and the selected excipients can be safely used. Dosage form studies were also completed with texture analysis and in vitro release with Franz diffusion chamber apparatus. Our results show that the ointments were able to reduce the extent of local inflammation in mice, but the exact mechanism of the effect remains unknown since BGP-15 did not show any antioxidant effect, nor was it able to decrease LPS-induced macrophage activation. Our results support the hypothesis that BGP-15 has a potential anti-inflammatory effect, even if it is topically applied, but the mechanism of the effect remains unclear and requires further pharmacological studies. LA - English DB - MTMT ER - TY - CONF AU - Bellák, Tamás AU - Pajer, Krisztián AU - Gál, László AU - Marton, Annamária AU - Csaba, Vizler AU - Fekécs, Zoltán AU - Nógrádi, Antal TI - Modulatory effects of grafted neuroectodermal stem cells after chronic spinal cord contusion injury T2 - Magyar Anatómus Társaság 2021. évi konferenciája PY - 2021 SP - 71 EP - 71 PG - 1 UR - https://m2.mtmt.hu/api/publication/32293885 ID - 32293885 LA - English DB - MTMT ER - TY - CONF AU - Bellák, Tamás AU - Pajer, Krisztián AU - Gál, László AU - Marton, Annamária AU - Vizler, Csaba AU - Fekécs, Zoltán AU - Nógrádi, Antal TI - Modulatory effects of grafted neuroectodermal stem cells after chronic spinal cord contusion injury T2 - Virtual FENS Regional Meeting 2021 PY - 2021 SP - 78 EP - 78 PG - 1 UR - https://m2.mtmt.hu/api/publication/32290397 ID - 32290397 LA - English DB - MTMT ER - TY - JOUR AU - Bajusz, Csaba AU - Kristó, Ildikó AU - Abonyi, Csilla AU - Venit, Tomáš AU - Vedelek, Viktor AU - Lukácsovich, Tamás AU - Farkas, Attila AU - Borkúti, Péter AU - Kovács, Zoltán AU - Bajusz, Izabella AU - Marton, Annamária AU - Vizler, Csaba AU - Lipinszki, Zoltán AU - Sinka, Rita AU - Percipalle, Piergiorgio AU - Vilmos, Péter TI - The nuclear activity of the actin‐binding Moesin protein is necessary for gene expression in Drosophila JF - FEBS JOURNAL J2 - FEBS J VL - 288 PY - 2021 IS - 16 SP - 4812 EP - 4832 PG - 21 SN - 1742-464X DO - 10.1111/febs.15779 UR - https://m2.mtmt.hu/api/publication/31909117 ID - 31909117 N1 - Eötvös Loránd Research Network (ELKH), Biological Research Centre, Szeged, Hungary Doctoral School of Biology, University of Szeged, Hungary Department of Genetics, University of Szeged, Hungary Doctoral School of Multidisciplinary Medical Science, University of Szeged, Hungary Lendület Laboratory of Cell Cycle Regulation, ELKH, Biological Research Centre, Szeged, Hungary LA - English DB - MTMT ER - TY - JOUR AU - Asperger, Hannah AU - Stamm, Nadia AU - Gierke, Berthold AU - Pawlak, Michael AU - Hofmann, Ute AU - Zanger, Ulrich M. AU - Marton, Annamária AU - Katona, Róbert László AU - Buhala, Andrea AU - Vizler, Csaba AU - Cieslik, Jan-Philipp AU - Kovacevic, Zaklina AU - Richardson, Des R. AU - Ruckhaeberle, Eugen AU - Niederacher, Dieter AU - Fehm, Tanja AU - Neubauer, Hans AU - Ludescher, Marina TI - Progesterone receptor membrane component 1 regulates lipid homeostasis and drives oncogenic signaling resulting in breast cancer progression (vol 22, 75, 2020) JF - BREAST CANCER RESEARCH J2 - BREAST CANCER RES VL - 23 PY - 2021 IS - 1 SN - 1465-5411 DO - 10.1186/s13058-020-01383-7 UR - https://m2.mtmt.hu/api/publication/31873459 ID - 31873459 AB - An amendment to this paper has been published and can be accessed via the original article. LA - English DB - MTMT ER -