TY - JOUR AU - Datki, Zsolt László AU - Balázs, Evelin AU - Gálik, Bence AU - Sinka, Rita AU - Zeitler, Lavínia AU - Bozsó, Zsolt AU - Kálmán, János AU - Hortobágyi, Tibor AU - Oláh, Zita TI - The interacting rotifer-biopolymers are anti- and disaggregating agents for human-type beta-amyloid in vitro JF - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES J2 - INT J BIOL MACROMOL VL - 201 PY - 2022 SP - 262 EP - 269 PG - 8 SN - 0141-8130 DO - 10.1016/j.ijbiomac.2021.12.184 UR - https://m2.mtmt.hu/api/publication/32591465 ID - 32591465 N1 - Funding Agency and Grant Number: Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences; New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Found [UNKP-21-5-SZTE-555]; European Union [754432]; Polish Ministry of Science and Higher Education; SZTEAOK-KKA [5S 567 (A202)]; Developing Scientific Workshops of Medical-, Health Sciences and Pharmaceutical Training (Hungary) [EFOP 3.6.3-VEKOP-16-201700009] Funding text: This project was supported by the J ' anos Bolyai Research Scholarship of the Hungarian Academy of Sciences; by the UNKP-21-5-SZTE-555 New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Found; by the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement, Nr. 754432; by the Polish Ministry of Science and Higher Education; by the SZTE ' AOK-KKA No. 5S 567 (A202) and by the Developing Scientific Workshops of Medical-, Health Sciences and Pharmaceutical Training (grant number: EFOP 3.6.3-VEKOP-16-201700009; Hungary). LA - English DB - MTMT ER - TY - JOUR AU - Balázs, Evelin AU - Oláh, Zita AU - Gálik, Bence AU - Somogyvári, Ferenc AU - Kálmán, János AU - Datki, Zsolt László TI - External modulation of Rotimer exudate secretion in monogonant rotifers JF - ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY J2 - ECOTOX ENVIRON SAFE VL - 220 PY - 2021 PG - 8 SN - 0147-6513 DO - 10.1016/j.ecoenv.2021.112399 UR - https://m2.mtmt.hu/api/publication/32059771 ID - 32059771 AB - The Rotimer, a rotifer-specific biopolymer, is an exogenic bioactive exudate secreted by different monogonant species (e.g. Euchlanis dilatata or Lecane bulla). The production of this viscoelastic biomolecule is induced by different micro-particles, thereby forming a special Rotimer-Inductor Conglomerate (RIC) in a web format. In this case, the water insoluble Carmine crystals, filtered to size (max. diameter was 50 µm), functioned as an inductor. The RIC production is an adequate empirical indicator to follow up this filamentous biopolymer secretion experientially; moreover, this procedure is very sensitive to the environmental factors (temperature, pH, metals and possible natural pollutant agents). The above mentioned species show completely different reactions to these factors, except to the presence of calcium and to the modulating effects of different drugs. One of the novelties of this work is that the Rotimer secretion and consequently, the RIC-formation is a mutually obligatory and evolutionary calcium-dependent process in the concerned monogonants. This in vivo procedure needs calcium, both for the physiology of animals and for fiber formation, particularly in the latter case. The conglomerate covered area (%) and the detection of the longest filament (mm) of the given RIC were the generally and simultaneously applied methods in the current modulating experiments. Exploring the regulatory (e.g. calcium-dependency) and stimulating (e.g. Lucidril effect) possibilities of biopolymer secretion are the basis for optimizing the RIC-production capacities of these micro-metazoans. LA - English DB - MTMT ER - TY - JOUR AU - Datki, Zsolt László AU - Ács, Éva AU - Balázs, Evelin AU - Sovány, Tamás AU - Pannonhalminé Csóka, Ildikó AU - Zsuga, Katalin AU - Kálmán, János AU - Oláh, Zita TI - Exogenic production of bioactive filamentous biopolymer by monogonant rotifers JF - ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY J2 - ECOTOX ENVIRON SAFE VL - 208 PY - 2021 PG - 10 SN - 0147-6513 DO - 10.1016/j.ecoenv.2020.111666 UR - https://m2.mtmt.hu/api/publication/31776926 ID - 31776926 N1 - A publikáció a Nemzeti Közszolgálati Egyetem 2020. évi Tématerületi Kiválóság Program keretében, a Fenntartható biztonság és társadalmi környezet elnevezésű projekt támogatásával valósult meg, az Innovációs és Technológiai Minisztérium Nemzeti Kutatási, Fejlesztési és Innovációs Alapból nyújtott támogatásával, a Nemzeti Kutatási, Fejlesztési és Innovációs Hivatal által kibocsátott támogatói okirat alapján. AB - The chemical ecology of rotifers has been little studied. A yet unknown property is presented within some monogonant rotifers, namely the ability to produce an exogenic filamentous biopolymer, named ‘Rotimer’. This rotifer-specific viscoelastic fiber was observed in six different freshwater monogonants (Euchlanis dilatata, Lecane bulla, Lepadella patella, Itura aurita, Colurella adriatica and Trichocerca iernis) in exception of four species. Induction of Rotimer secretion can only be achieved by mechanically irritating rotifer ciliate with administering different types (yeast cell skeleton, denatured BSA, epoxy, Carmine or urea crystals and micro-cellulose) and sizes (approx. from 2.5 to 50 μm diameter) of inert particles, as inductors or visualization by adhering particles. The thickness of this Rotimer is 33 ± 3 nm, detected by scanning electron microscope. This material has two structural formations (fiber or gluelike) in nano dimension. The existence of the novel adherent natural product becomes visible by forming a ‘Rotimer-Inductor Conglomerate’ (RIC) web structure within a few minutes. The RIC-producing capacity of animals, depends on viability, is significantly modified according to physiological-(depletion), drug- (toxin or stimulator) and environmental (temperature, salt content and pH) effects. The E. dilatata-produced RIC is affected by protein disruptors but is resistant to several chemical influences and its Rotimer component has an overwhelming cell (algae, yeast and human neuroblastoma) motility inhibitory effect, associated with low toxicity. This biopolymer-secretion-capacity is protective of rotifers against human-type beta-amyloid aggregates. LA - English DB - MTMT ER - TY - JOUR AU - Balázs, Evelin AU - Oláh, Zita AU - Gálik, Bence AU - Bozsó, Zsolt AU - Kálmán, János AU - Datki, Zsolt László TI - Neurodegeneration-related beta-amyloid as autocatabolism-attenuator in a micro-in vivo system JF - IBRO REPORTS J2 - IBRO REP VL - 9 PY - 2020 SP - 319 EP - 323 PG - 5 SN - 2451-8301 DO - 10.1016/j.ibror.2020.10.002 UR - https://m2.mtmt.hu/api/publication/31639846 ID - 31639846 LA - English DB - MTMT ER - TY - JOUR AU - Datki, Zsolt László AU - Oláh, Zita AU - Jánosi-Mózes, Emese AU - Szegedi, Viktor AU - Kálmán, János AU - Hunya, Ákos AU - Fülöp, Lívia AU - Tamano, Haruna AU - Takeda, Atsushi AU - Adlard, Paul A. AU - Bush, Ashley I. TI - Alzheimer risk factors age and female sex induce cortical Aβ aggregation by raising extracellular zinc [Alzheimer risk factors age and female sex induce cortical A beta aggregation by raising extracellular zinc] JF - MOLECULAR PSYCHIATRY J2 - MOL PSYCHIATR VL - 25 PY - 2020 IS - 11 SP - 2728 EP - 2741 PG - 14 SN - 1359-4184 DO - 10.1038/s41380-020-0800-y UR - https://m2.mtmt.hu/api/publication/31341681 ID - 31341681 AB - Aging and female sex are the major risk factors for Alzheimer's disease and its associated brain amyloid-beta (A beta) neuropathology, but the mechanisms mediating these risk factors remain uncertain. Evidence indicates that A beta aggregation by Zn(2+)released from glutamatergic neurons contributes to amyloid neuropathology, so we tested whether aging and sex adversely influences this neurophysiology. Using acute hippocampal slices, we found that extracellular Zn2+-elevation induced by high K(+)stimulation was significantly greater with older (65 weeks vs 10 weeks old) rats, and was exaggerated in females. This was driven by slower reuptake of extracellular Zn2+, which could be recapitulated by mitochondrial intoxication. Zn2+:A beta aggregates were toxic to the slices, but A beta alone was not. Accordingly, high K(+)caused synthetic human A beta added to the slices to form soluble oligomers as detected by bis-ANS, attaching to neurons and inducing toxicity, with older slices being more vulnerable. Age-dependent energy failure impairing Zn(2+)reuptake, and a higher maximal capacity for Zn(2+)release by females, could contribute to age and sex being major risk factors for Alzheimer's disease. LA - English DB - MTMT ER - TY - JOUR AU - Datki, Zsolt László AU - Oláh, Zita AU - Borbélyné Mácsai, Lilla AU - Pákáski, Magdolna AU - Gálik, Bence AU - Mihaly, Gabor AU - Kálmán, János TI - Application of BisANS fluorescent dye for developing a novel protein assay JF - PLOS ONE J2 - PLOS ONE VL - 14 PY - 2019 IS - 4 PG - 8 SN - 1932-6203 DO - 10.1371/journal.pone.0215863 UR - https://m2.mtmt.hu/api/publication/30812390 ID - 30812390 LA - English DB - MTMT ER - TY - JOUR AU - Datki, Zsolt László AU - Oláh, Zita AU - Bohár, Zsuzsanna AU - Zádori, Dénes AU - Fülöp, Ferenc AU - Szatmári, István AU - Gálik, Bence AU - Kálmán, János AU - Vécsei, László TI - Kynurenic Acid and Its Analogs Are Beneficial Physiologic Attenuators in Bdelloid Rotifers JF - MOLECULES J2 - MOLECULES VL - 24 PY - 2019 IS - 11 PG - 10 SN - 1420-3049 DO - 10.3390/molecules24112171 UR - https://m2.mtmt.hu/api/publication/30759846 ID - 30759846 N1 - Funding Agency and Grant Number: Ministry of Human Capacities, Hungary [20391-3/2018/FEKUSTRAT]; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences; [GINOP 2.3.2-15-2016-00034] Funding text: This research was funded by the project GINOP 2.3.2-15-2016-00034 and by the Ministry of Human Capacities, Hungary grant 20391-3/2018/FEKUSTRAT. DZ was supported by Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences. Department of Psychiatry, Faculty of Medicine, University of Szeged, Kalvaria sgt. 57, Szeged, H-6725, Hungary MTA-SZTE Neuroscience Research Group, Semmelweis u. 6, Szeged, H-6725, Hungary Department of Neurology, Interdisciplinary Excellence Centre, Faculty of Medicine, University of Szeged, Semmelweis u. 6, Szeged, H-6725, Hungary Institute of Pharmaceutical Chemistry, Interdisciplinary Excellence Centre, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, Szeged, H-6720, Hungary MTA-SZTE Stereochemistry Research Group, Eötvös u. 6, Szeged, H-6720, Hungary Department of Clinical Molecular Biology, Medical University of Bialystok, ul.Jana Kilinskiego 1, Bialystok, 15-089, Poland Export Date: 27 August 2019 CODEN: MOLEF Correspondence Address: Datki, Z.; Department of Psychiatry, Faculty of Medicine, University of Szeged, Kalvaria sgt. 57, Hungary; email: datkizsolt@gmail.com Funding details: Emberi Eroforrások Minisztériuma, 20391-3/2018/FEKUSTRAT Funding details: Magyar Tudományos Akadémia Funding text 1: Funding: This research was funded by the project GINOP 2.3.2-15-2016-00034 and by the Ministry of Human Capacities, Hungary grant 20391-3/2018/FEKUSTRAT. DZ was supported by János Bolyai Research Scholarship of the Hungarian Academy of Sciences. Department of Psychiatry, Faculty of Medicine, University of Szeged, Kalvaria sgt. 57, Szeged, H-6725, Hungary MTA-SZTE Neuroscience Research Group, Semmelweis u. 6, Szeged, H-6725, Hungary Department of Neurology, Interdisciplinary Excellence Centre, Faculty of Medicine, University of Szeged, Semmelweis u. 6, Szeged, H-6725, Hungary Institute of Pharmaceutical Chemistry, Interdisciplinary Excellence Centre, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, Szeged, H-6720, Hungary MTA-SZTE Stereochemistry Research Group, Eötvös u. 6, Szeged, H-6720, Hungary Department of Clinical Molecular Biology, Medical University of Bialystok, ul.Jana Kilinskiego 1, Bialystok, 15-089, Poland Export Date: 12 September 2019 CODEN: MOLEF Correspondence Address: Datki, Z.; Department of Psychiatry, Faculty of Medicine, University of Szeged, Kalvaria sgt. 57, Hungary; email: datkizsolt@gmail.com Funding details: Emberi Eroforrások Minisztériuma, 20391-3/2018/FEKUSTRAT Funding details: Magyar Tudományos Akadémia Funding text 1: Funding: This research was funded by the project GINOP 2.3.2-15-2016-00034 and by the Ministry of Human Capacities, Hungary grant 20391-3/2018/FEKUSTRAT. DZ was supported by János Bolyai Research Scholarship of the Hungarian Academy of Sciences. Department of Psychiatry, Faculty of Medicine, University of Szeged, Kalvaria sgt. 57, Szeged, H-6725, Hungary MTA-SZTE Neuroscience Research Group, Semmelweis u. 6, Szeged, H-6725, Hungary Department of Neurology, Interdisciplinary Excellence Centre, Faculty of Medicine, University of Szeged, Semmelweis u. 6, Szeged, H-6725, Hungary Institute of Pharmaceutical Chemistry, Interdisciplinary Excellence Centre, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, Szeged, H-6720, Hungary MTA-SZTE Stereochemistry Research Group, Eötvös u. 6, Szeged, H-6720, Hungary Department of Clinical Molecular Biology, Medical University of Bialystok, ul.Jana Kilinskiego 1, Bialystok, 15-089, Poland Export Date: 13 September 2019 CODEN: MOLEF Correspondence Address: Datki, Z.; Department of Psychiatry, Faculty of Medicine, University of Szeged, Kalvaria sgt. 57, Hungary; email: datkizsolt@gmail.com Funding details: Emberi Eroforrások Minisztériuma, 20391-3/2018/FEKUSTRAT Funding details: Magyar Tudományos Akadémia Funding text 1: Funding: This research was funded by the project GINOP 2.3.2-15-2016-00034 and by the Ministry of Human Capacities, Hungary grant 20391-3/2018/FEKUSTRAT. DZ was supported by János Bolyai Research Scholarship of the Hungarian Academy of Sciences. AB - The in vivo investigation of kynurenic acid (KYNA) and its analogs is one of the recent exciting topics in pharmacology. In the current study we assessed the biological effects of these molecules on bdelloid rotifers (Philodina acuticornis and Adineta vaga) by monitoring changes in their survival and phenotypical characteristics. In addition to longitudinal (slowly changing) markers (survival, number of rotifers alive and body size index), some dynamic (quickly responding) ones (cellular reduction capacity and mastax contraction frequency) were measured as well. KYNA and its analogs increased longevity, reproduction and growth, whereas reduction capacity and energy-dependent muscular activity decreased conversely. We found that spermidine, a calorie restriction mimetic, exerted similar changes in the applied micro-invertebrates. This characterized systemic profile evoked by the above-mentioned compounds was named beneficial physiologic attenuation. In reference experiments, using a stimulator (cyclic adenosine monophosphate) and a toxin (sodium azide), all parameters changed in the same direction (positively or negatively, respectively), as expected. The currently described adaptive phenomenon in bdelloid rotifers may provide holistic perspectives in translational research. LA - English DB - MTMT ER - TY - JOUR AU - Borbélyné Mácsai, Lilla AU - Oláh, Zita AU - Bush, Ashley I AU - Gálik, Bence AU - Ónody, Rita AU - Kálmán, János AU - Datki, Zsolt László TI - Redox Modulating Factors Affect Longevity Regulation in Rotifers JF - JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES J2 - J GERONTOL A-BIOL MED SCI VL - 74 PY - 2019 IS - 6 SP - 811 EP - 814 PG - 4 SN - 1079-5006 DO - 10.1093/gerona/gly193 UR - https://m2.mtmt.hu/api/publication/30626843 ID - 30626843 LA - English DB - MTMT ER - TY - THES AU - Oláh, Zita TI - The relation between human biomarkers and oxidative stress in Alzheimer’s disease, novel in vivo experimental modelling possibilities PB - Szegedi Tudományegyetem (SZTE) PY - 2018 SP - 69 DO - 10.14232/phd.9739 UR - https://m2.mtmt.hu/api/publication/30612504 ID - 30612504 LA - English DB - MTMT ER - TY - JOUR AU - Datki, Zsolt László AU - Oláh, Zita AU - Hortobágyi, Tibor AU - Borbélyné Mácsai, Lilla AU - Zsuga, Katalin AU - Fülöp, Lívia AU - Bozsó, Zsolt AU - Gálik, Bence AU - Ács, Éva AU - Földi, Angéla AU - Szarvas, A AU - Kálmán, János TI - Exceptional in vivo catabolism of neurodegeneration-related aggregates JF - ACTA NEUROPATHOLOGICA COMMUNICATIONS J2 - ACTA NEUROPATH COMM VL - 6 PY - 2018 IS - 1 PG - 12 SN - 2051-5960 DO - 10.1186/s40478-018-0507-3 UR - https://m2.mtmt.hu/api/publication/3323000 ID - 3323000 LA - English DB - MTMT ER -