@article{MTMT:32591465, title = {The interacting rotifer-biopolymers are anti- and disaggregating agents for human-type beta-amyloid in vitro}, url = {https://m2.mtmt.hu/api/publication/32591465}, author = {Datki, Zsolt László and Balázs, Evelin and Gálik, Bence and Sinka, Rita and Zeitler, Lavínia and Bozsó, Zsolt and Kálmán, János and Hortobágyi, Tibor and Oláh, Zita}, doi = {10.1016/j.ijbiomac.2021.12.184}, journal-iso = {INT J BIOL MACROMOL}, journal = {INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES}, volume = {201}, unique-id = {32591465}, issn = {0141-8130}, year = {2022}, eissn = {1879-0003}, pages = {262-269}, orcid-numbers = {Datki, Zsolt László/0000-0002-2537-4741; Sinka, Rita/0000-0003-4040-4184; Bozsó, Zsolt/0000-0002-5713-3096; Kálmán, János/0000-0001-5319-5639; Hortobágyi, Tibor/0000-0001-5732-7942; Oláh, Zita/0000-0002-6372-532X} } @article{MTMT:32059771, title = {External modulation of Rotimer exudate secretion in monogonant rotifers}, url = {https://m2.mtmt.hu/api/publication/32059771}, author = {Balázs, Evelin and Oláh, Zita and Gálik, Bence and Somogyvári, Ferenc and Kálmán, János and Datki, Zsolt László}, doi = {10.1016/j.ecoenv.2021.112399}, journal-iso = {ECOTOX ENVIRON SAFE}, journal = {ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY}, volume = {220}, unique-id = {32059771}, issn = {0147-6513}, abstract = {The Rotimer, a rotifer-specific biopolymer, is an exogenic bioactive exudate secreted by different monogonant species (e.g. Euchlanis dilatata or Lecane bulla). The production of this viscoelastic biomolecule is induced by different micro-particles, thereby forming a special Rotimer-Inductor Conglomerate (RIC) in a web format. In this case, the water insoluble Carmine crystals, filtered to size (max. diameter was 50 µm), functioned as an inductor. The RIC production is an adequate empirical indicator to follow up this filamentous biopolymer secretion experientially; moreover, this procedure is very sensitive to the environmental factors (temperature, pH, metals and possible natural pollutant agents). The above mentioned species show completely different reactions to these factors, except to the presence of calcium and to the modulating effects of different drugs. One of the novelties of this work is that the Rotimer secretion and consequently, the RIC-formation is a mutually obligatory and evolutionary calcium-dependent process in the concerned monogonants. This in vivo procedure needs calcium, both for the physiology of animals and for fiber formation, particularly in the latter case. The conglomerate covered area (%) and the detection of the longest filament (mm) of the given RIC were the generally and simultaneously applied methods in the current modulating experiments. Exploring the regulatory (e.g. calcium-dependency) and stimulating (e.g. Lucidril effect) possibilities of biopolymer secretion are the basis for optimizing the RIC-production capacities of these micro-metazoans.}, keywords = {biopolymer; exudate; Monogonant rotifer; Euchlanis dilatata; Ascorbic acid (PubChem CID: 54670067); CaCl(2) (PubChem CID: 24844); Carmine crystals (PubChem CID: 14950); Catechin (PubChem CID: 9064); Choline chloride (PubChem CID: 6209); DMSO (PubChem CID: 679); EDTA (PubChem CID: 6049); Ethyl nicotinate (PubChem CID: 69188); Glucose (PubChem CID: 5793); Glycerol (PubChem CID: 753); Isoguvacine (PubChem CID: 3765); KCl (PubChem CID: 4873); Lecane bulla; LiCl (PubChem CID: 23681138); Meclofenoxate hydrochloride (PubChem CID: 19379); MgCl(2) (PubChem CID: 24644); NaCl (PubChem CID: 5234); Rotimer; Selegiline (PubChem CID: 26757); Spermidine (PubChem CID: 1102); SrCl(2) (PubChem CID: 6101868); Taurine (PubChem CID: 1123); cAMP (PubChem CID: 6076)}, year = {2021}, eissn = {1090-2414}, orcid-numbers = {Oláh, Zita/0000-0002-6372-532X; Kálmán, János/0000-0001-5319-5639; Datki, Zsolt László/0000-0002-2537-4741} } @article{MTMT:31776926, title = {Exogenic production of bioactive filamentous biopolymer by monogonant rotifers}, url = {https://m2.mtmt.hu/api/publication/31776926}, author = {Datki, Zsolt László and Ács, Éva and Balázs, Evelin and Sovány, Tamás and Pannonhalminé Csóka, Ildikó and Zsuga, Katalin and Kálmán, János and Oláh, Zita}, doi = {10.1016/j.ecoenv.2020.111666}, journal-iso = {ECOTOX ENVIRON SAFE}, journal = {ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY}, volume = {208}, unique-id = {31776926}, issn = {0147-6513}, abstract = {The chemical ecology of rotifers has been little studied. A yet unknown property is presented within some monogonant rotifers, namely the ability to produce an exogenic filamentous biopolymer, named ‘Rotimer’. This rotifer-specific viscoelastic fiber was observed in six different freshwater monogonants (Euchlanis dilatata, Lecane bulla, Lepadella patella, Itura aurita, Colurella adriatica and Trichocerca iernis) in exception of four species. Induction of Rotimer secretion can only be achieved by mechanically irritating rotifer ciliate with administering different types (yeast cell skeleton, denatured BSA, epoxy, Carmine or urea crystals and micro-cellulose) and sizes (approx. from 2.5 to 50 μm diameter) of inert particles, as inductors or visualization by adhering particles. The thickness of this Rotimer is 33 ± 3 nm, detected by scanning electron microscope. This material has two structural formations (fiber or gluelike) in nano dimension. The existence of the novel adherent natural product becomes visible by forming a ‘Rotimer-Inductor Conglomerate’ (RIC) web structure within a few minutes. The RIC-producing capacity of animals, depends on viability, is significantly modified according to physiological-(depletion), drug- (toxin or stimulator) and environmental (temperature, salt content and pH) effects. The E. dilatata-produced RIC is affected by protein disruptors but is resistant to several chemical influences and its Rotimer component has an overwhelming cell (algae, yeast and human neuroblastoma) motility inhibitory effect, associated with low toxicity. This biopolymer-secretion-capacity is protective of rotifers against human-type beta-amyloid aggregates.}, keywords = {biopolymer; exudate; Monogonant rotifer; Euchlanis dilatata; SH-SY5Y neuroblastoma cell line; Beta-amyloid; Keywords: Monogonant rotifer Biopolymer Euchlanis dilatata SH-SY5Y neuroblastoma cell line Exudate Beta-amyloid}, year = {2021}, eissn = {1090-2414}, orcid-numbers = {Datki, Zsolt László/0000-0002-2537-4741; Ács, Éva/0000-0003-1774-157X; Sovány, Tamás/0000-0003-3392-7788; Pannonhalminé Csóka, Ildikó/0000-0003-0807-2781; Kálmán, János/0000-0001-5319-5639; Oláh, Zita/0000-0002-6372-532X} } @article{MTMT:31639846, title = {Neurodegeneration-related beta-amyloid as autocatabolism-attenuator in a micro-in vivo system}, url = {https://m2.mtmt.hu/api/publication/31639846}, author = {Balázs, Evelin and Oláh, Zita and Gálik, Bence and Bozsó, Zsolt and Kálmán, János and Datki, Zsolt László}, doi = {10.1016/j.ibror.2020.10.002}, journal-iso = {IBRO REP}, journal = {IBRO REPORTS}, volume = {9}, unique-id = {31639846}, year = {2020}, eissn = {2451-8301}, pages = {319-323}, orcid-numbers = {Oláh, Zita/0000-0002-6372-532X; Bozsó, Zsolt/0000-0002-5713-3096; Kálmán, János/0000-0001-5319-5639; Datki, Zsolt László/0000-0002-2537-4741} } @article{MTMT:31341681, title = {Alzheimer risk factors age and female sex induce cortical Aβ aggregation by raising extracellular zinc [Alzheimer risk factors age and female sex induce cortical A beta aggregation by raising extracellular zinc]}, url = {https://m2.mtmt.hu/api/publication/31341681}, author = {Datki, Zsolt László and Oláh, Zita and Jánosi-Mózes, Emese and Szegedi, Viktor and Kálmán, János and Hunya, Ákos and Fülöp, Lívia and Tamano, Haruna and Takeda, Atsushi and Adlard, Paul A. and Bush, Ashley I.}, doi = {10.1038/s41380-020-0800-y}, journal-iso = {MOL PSYCHIATR}, journal = {MOLECULAR PSYCHIATRY}, volume = {25}, unique-id = {31341681}, issn = {1359-4184}, abstract = {Aging and female sex are the major risk factors for Alzheimer's disease and its associated brain amyloid-beta (A beta) neuropathology, but the mechanisms mediating these risk factors remain uncertain. Evidence indicates that A beta aggregation by Zn(2+)released from glutamatergic neurons contributes to amyloid neuropathology, so we tested whether aging and sex adversely influences this neurophysiology. Using acute hippocampal slices, we found that extracellular Zn2+-elevation induced by high K(+)stimulation was significantly greater with older (65 weeks vs 10 weeks old) rats, and was exaggerated in females. This was driven by slower reuptake of extracellular Zn2+, which could be recapitulated by mitochondrial intoxication. Zn2+:A beta aggregates were toxic to the slices, but A beta alone was not. Accordingly, high K(+)caused synthetic human A beta added to the slices to form soluble oligomers as detected by bis-ANS, attaching to neurons and inducing toxicity, with older slices being more vulnerable. Age-dependent energy failure impairing Zn(2+)reuptake, and a higher maximal capacity for Zn(2+)release by females, could contribute to age and sex being major risk factors for Alzheimer's disease.}, year = {2020}, eissn = {1476-5578}, pages = {2728-2741}, orcid-numbers = {Datki, Zsolt László/0000-0002-2537-4741; Oláh, Zita/0000-0002-6372-532X; Jánosi-Mózes, Emese/0000-0003-1532-289X; Szegedi, Viktor/0000-0003-4191-379X; Kálmán, János/0000-0001-5319-5639; Hunya, Ákos/0000-0002-4547-9284; Fülöp, Lívia/0000-0002-8010-0129} } @article{MTMT:30812390, title = {Application of BisANS fluorescent dye for developing a novel protein assay}, url = {https://m2.mtmt.hu/api/publication/30812390}, author = {Datki, Zsolt László and Oláh, Zita and Borbélyné Mácsai, Lilla and Pákáski, Magdolna and Gálik, Bence and Mihaly, Gabor and Kálmán, János}, doi = {10.1371/journal.pone.0215863}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {14}, unique-id = {30812390}, issn = {1932-6203}, year = {2019}, eissn = {1932-6203}, orcid-numbers = {Datki, Zsolt László/0000-0002-2537-4741; Oláh, Zita/0000-0002-6372-532X; Borbélyné Mácsai, Lilla/0000-0002-9503-6449; Pákáski, Magdolna/0000-0001-8067-5435; Kálmán, János/0000-0001-5319-5639} } @article{MTMT:30759846, title = {Kynurenic Acid and Its Analogs Are Beneficial Physiologic Attenuators in Bdelloid Rotifers}, url = {https://m2.mtmt.hu/api/publication/30759846}, author = {Datki, Zsolt László and Oláh, Zita and Bohár, Zsuzsanna and Zádori, Dénes and Fülöp, Ferenc and Szatmári, István and Gálik, Bence and Kálmán, János and Vécsei, László}, doi = {10.3390/molecules24112171}, journal-iso = {MOLECULES}, journal = {MOLECULES}, volume = {24}, unique-id = {30759846}, issn = {1420-3049}, abstract = {The in vivo investigation of kynurenic acid (KYNA) and its analogs is one of the recent exciting topics in pharmacology. In the current study we assessed the biological effects of these molecules on bdelloid rotifers (Philodina acuticornis and Adineta vaga) by monitoring changes in their survival and phenotypical characteristics. In addition to longitudinal (slowly changing) markers (survival, number of rotifers alive and body size index), some dynamic (quickly responding) ones (cellular reduction capacity and mastax contraction frequency) were measured as well. KYNA and its analogs increased longevity, reproduction and growth, whereas reduction capacity and energy-dependent muscular activity decreased conversely. We found that spermidine, a calorie restriction mimetic, exerted similar changes in the applied micro-invertebrates. This characterized systemic profile evoked by the above-mentioned compounds was named beneficial physiologic attenuation. In reference experiments, using a stimulator (cyclic adenosine monophosphate) and a toxin (sodium azide), all parameters changed in the same direction (positively or negatively, respectively), as expected. The currently described adaptive phenomenon in bdelloid rotifers may provide holistic perspectives in translational research.}, keywords = {LIFE-SPAN; metabolism; SURVIVAL; physiology; kynurenic acid; Biochemistry & Molecular Biology; CALORIE RESTRICTION; bdelloid rotifer}, year = {2019}, eissn = {1420-3049}, orcid-numbers = {Datki, Zsolt László/0000-0002-2537-4741; Oláh, Zita/0000-0002-6372-532X; Zádori, Dénes/0000-0003-0749-7980; Fülöp, Ferenc/0000-0003-1066-5287; Szatmári, István/0000-0002-8571-5229; Kálmán, János/0000-0001-5319-5639; Vécsei, László/0000-0001-8037-3672} } @article{MTMT:30626843, title = {Redox Modulating Factors Affect Longevity Regulation in Rotifers}, url = {https://m2.mtmt.hu/api/publication/30626843}, author = {Borbélyné Mácsai, Lilla and Oláh, Zita and Bush, Ashley I and Gálik, Bence and Ónody, Rita and Kálmán, János and Datki, Zsolt László}, doi = {10.1093/gerona/gly193}, journal-iso = {J GERONTOL A-BIOL MED SCI}, journal = {JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES}, volume = {74}, unique-id = {30626843}, issn = {1079-5006}, year = {2019}, eissn = {1758-535X}, pages = {811-814}, orcid-numbers = {Borbélyné Mácsai, Lilla/0000-0002-9503-6449; Oláh, Zita/0000-0002-6372-532X; Kálmán, János/0000-0001-5319-5639; Datki, Zsolt László/0000-0002-2537-4741} } @mastersthesis{MTMT:30612504, title = {The relation between human biomarkers and oxidative stress in Alzheimer’s disease, novel in vivo experimental modelling possibilities}, url = {https://m2.mtmt.hu/api/publication/30612504}, author = {Oláh, Zita}, doi = {10.14232/phd.9739}, publisher = {SZTE}, unique-id = {30612504}, year = {2018}, orcid-numbers = {Oláh, Zita/0000-0002-6372-532X} } @article{MTMT:3323000, title = {Exceptional in vivo catabolism of neurodegeneration-related aggregates}, url = {https://m2.mtmt.hu/api/publication/3323000}, author = {Datki, Zsolt László and Oláh, Zita and Hortobágyi, Tibor and Borbélyné Mácsai, Lilla and Zsuga, Katalin and Fülöp, Lívia and Bozsó, Zsolt and Gálik, Bence and Ács, Éva and Földi, Angéla and Szarvas, A and Kálmán, János}, doi = {10.1186/s40478-018-0507-3}, journal-iso = {ACTA NEUROPATH COMM}, journal = {ACTA NEUROPATHOLOGICA COMMUNICATIONS}, volume = {6}, unique-id = {3323000}, issn = {2051-5960}, year = {2018}, eissn = {2051-5960}, orcid-numbers = {Datki, Zsolt László/0000-0002-2537-4741; Oláh, Zita/0000-0002-6372-532X; Hortobágyi, Tibor/0000-0001-5732-7942; Borbélyné Mácsai, Lilla/0000-0002-9503-6449; Fülöp, Lívia/0000-0002-8010-0129; Bozsó, Zsolt/0000-0002-5713-3096; Ács, Éva/0000-0003-1774-157X; Kálmán, János/0000-0001-5319-5639} }