TY - JOUR AU - Sándor, Viktor AU - Berkics, Balázs Viktor AU - Kilár, Anikó AU - Kocsis, Béla AU - Kilár, Ferenc AU - Dörnyei, Ágnes TI - NACE-ESI-MS/MS method for separation and characterization of phosphorylation and acylation isomers of lipid A JF - ELECTROPHORESIS J2 - ELECTROPHORESIS VL - 41 PY - 2020 IS - 13-14 SP - 1178 EP - 1188 PG - 11 SN - 0173-0835 DO - 10.1002/elps.201900251 UR - https://m2.mtmt.hu/api/publication/31292418 ID - 31292418 N1 - * Megosztott szerzőség AB - Lipid A represents a heterogeneous group of bacterial outer membrane phosphoglycolipids, which play a major role in the pathogenesis of Gram-negative sepsis. The number and position of phosphoryl and acyl groups in lipid A molecules are key structural determinants in their bioactivities. In this study, a NACE-ESI-MS/MS method was developed for the simultaneous analysis of lipid A isomers possessing a different degree of phosphorylation and acylation. Various C4'- and C1-monophosphorylated lipid A isobars, as well as acylation isomers, were baseline separated within 43 min, in a separation medium of methanol/dichloromethane/triethylamine/acetic acid 60:40:1.08:0.36 (v/v/v/v). Both normal and reverse CE polarities could be applied for proper detection of the analytes owing to the combination of a suction effect caused by the nebulizer gas at the outlet end of the capillary and external pressure applied on the inlet vial. The separated lipid A species could be identified unequivocally by their characteristic fragmentation patterns through CID performed in both negative- and positive-ionization modes. The uniqueness of the NACE-ESI-MS/MS method lies in its simplicity and reliability for proving the phosphorylation isomerism (C1 or C4') and acylation pattern of native lipid A species or those designed for therapeutic applications. This article is protected by copyright. All rights reserved. LA - English DB - MTMT ER - TY - CHAP AU - Sándor, Viktor AU - Kilár, Anikó AU - Berkics, Balázs Viktor AU - Kocsis, Béla AU - Kilár, Ferenc AU - Dörnyei, Ágnes ED - Hungarian, Society for Separation Science TI - Comparison of LC-MS and CE MS for the analysis of immunstimulant bacterial membrane glycolipids T2 - 12th Balaton Symposium on High-Performance Separation Methods PB - Hungarian Society for Separation Sciences CY - Pécs SN - 9786155270574 PY - 2019 SP - 105 UR - https://m2.mtmt.hu/api/publication/30816329 ID - 30816329 LA - English DB - MTMT ER - TY - JOUR AU - Ivanova-Petropulos, V AU - Naceva, Z AU - Sándor, Viktor AU - Makszin, Lilla AU - Nagy, Laura AU - Berkics, Balázs Viktor AU - Stafilov, T AU - Kilár, Ferenc TI - Fast determination of lactic, succinic, malic, tartaric, shikimic and citric acids in red Vranec wines by CZE-ESI-QTOF-MS JF - ELECTROPHORESIS J2 - ELECTROPHORESIS VL - 39 PY - 2018 IS - 13 SP - 1597 EP - 1605 PG - 9 SN - 0173-0835 DO - 10.1002/elps.201700492 UR - https://m2.mtmt.hu/api/publication/3360068 ID - 3360068 AB - A fast and simple method with capillary zone electrophoresis coupled to electrospray ionization/quadrupole-time-of-flight-mass spectrometry (CZE-ESI/QTOF-MS) was optimized and validated for quantitative determination of organic acids (lactic acid, succinic acid, malic acid, tartaric acid, shikimic acid and citric acid) in red wines. The background electrolyte was ammonium acetate and the separation of the analytes was performed in a polybrene-coated capillary in the presence of electroosmotic flow. The sample preparation included dilution and filtration of the wine. The method showed satisfactory performance characteristics: good linearity for each organic acid, with correlation coefficients ranging from r(2) = 0.9902 (shikimic acid) to r(2) = 0.9990 (tartaric acid). The limit of quantification was between 0.0034 mM (for shikimic acid) and 0.107 mM (for citric acid), and the recovery data fell between 95.8 % (malic acid) and 102.7 % (lactic acid); the total run time was less than 4 minutes. The RSD values for the inter-day repeatability and intra-day reproducibility were between 3.44 and 9.50 %, and between 1.75 and 8.29 %, respectively. Seventeen Macedonian red Vranec wines were studied demonstrating a wide variation in the organic acids' concentration, which should be most probably due to the variation of the climate conditions in the vine areas. This article is protected by copyright. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Páger, Csilla AU - Biherczová, Nikoleta AU - Ligetvári, Roland AU - Berkics, Balázs Viktor AU - Pongrácz, Tamás AU - Sándor, Viktor AU - Bufa, Anita AU - Poór, Viktória AU - Vojs Staňová, Andrea AU - Kilár, Ferenc TI - Advanced on-line mass spectrometry detection of proteins separated by capillary isoelectric focusing after sequential injection JF - JOURNAL OF SEPARATION SCIENCE J2 - J SEP SCI VL - 40 PY - 2017 IS - 24 SP - 4825 EP - 4834 PG - 10 SN - 1615-9306 DO - 10.1002/jssc.201700695 UR - https://m2.mtmt.hu/api/publication/3276390 ID - 3276390 N1 - Funding Agency and Grant Number: Slovak Grant Agency for Science [VEGA 1/0342/15]; [APVV-0583-11]; [VVCE-0070-07]; [CEEPUS-III-RO-0010]; [PTE-AOK-KA-2017-19]; [NKFIH-K-125275] Funding text: The authors are thankful to project APVV-0583-11 for financial support within the frame of VVCE-0070-07; the Slovak Grant Agency for Science (VEGA 1/0342/15); and the CEEPUS-III-RO-0010 Network. The work was supported by the grants PTE-AOK-KA-2017-19 and NKFIH-K-125275. LA - English DB - MTMT ER - TY - CHAP AU - Páger, Csilla AU - Biherczova, Nikoleta AU - Ligetvári, Roland AU - Berkics, Balázs Viktor AU - Sándor, Viktor AU - Kilár, Ferenc TI - Kapilláris izoelektromos fókuszálás alkalmazása tömegspektrometriás detektálással fehérjekeverék elválasztására T2 - Elválasztástudományi Vándorgyűlés 2016 PB - Magyar Elválasztástudományi Társaság CY - Pécs SN - 9786155270307 PY - 2016 UR - https://m2.mtmt.hu/api/publication/3139957 ID - 3139957 LA - Hungarian DB - MTMT ER - TY - CONF AU - Violeta, Ivanova - Petropulos AU - Zaneta, Naceva AU - Sándor, Viktor AU - Berkics, Balázs Viktor AU - Trajce, Stafilov AU - Kilár, Ferenc ED - [sn], null TI - Determination of Organic Acids in Wines Using Capillary Zone Electrophoresis - Electrospray Ionization / Quadrupole - Time - of - Flight - Mass Spectrometry (CZE - ESI/QTOF - MS) T2 - 16th CEEPUS Symposium and Summer School on Bioanalysis PB - University of Warsaw C1 - Warsaw PY - 2016 SP - 22 UR - https://m2.mtmt.hu/api/publication/3093098 ID - 3093098 LA - English DB - MTMT ER - TY - CONF AU - Berkics, Balázs Viktor AU - Pongrácz, Tamás AU - Poór, Viktória AU - Sándor, Viktor AU - Csóka, Balázs AU - Páger, Csilla AU - Kilár, Ferenc ED - Gazdag, Mária ED - Felinger, Attila ED - Babják, Mónika ED - Drahos, László ED - Horváth, Krisztián ED - Janáky, Tamás ED - Móricz, Ágnes TI - Nitrofenol festékek és fehérjék elválasztása kapilláris izoelektromos fókuszálással, izoelektromos fókuszálás kapcsolása tömegspektrométerre T2 - Elválasztástudományi Vándorgyűlés 2014 PB - Magyar Elválasztástudományi Társaság C1 - Pécs PY - 2014 SP - 79 UR - https://m2.mtmt.hu/api/publication/2775516 ID - 2775516 LA - Hungarian DB - MTMT ER -