@article{MTMT:31292418, title = {NACE-ESI-MS/MS method for separation and characterization of phosphorylation and acylation isomers of lipid A}, url = {https://m2.mtmt.hu/api/publication/31292418}, author = {Sándor, Viktor and Berkics, Balázs Viktor and Kilár, Anikó and Kocsis, Béla and Kilár, Ferenc and Dörnyei, Ágnes}, doi = {10.1002/elps.201900251}, journal-iso = {ELECTROPHORESIS}, journal = {ELECTROPHORESIS}, volume = {41}, unique-id = {31292418}, issn = {0173-0835}, abstract = {Lipid A represents a heterogeneous group of bacterial outer membrane phosphoglycolipids, which play a major role in the pathogenesis of Gram-negative sepsis. The number and position of phosphoryl and acyl groups in lipid A molecules are key structural determinants in their bioactivities. In this study, a NACE-ESI-MS/MS method was developed for the simultaneous analysis of lipid A isomers possessing a different degree of phosphorylation and acylation. Various C4'- and C1-monophosphorylated lipid A isobars, as well as acylation isomers, were baseline separated within 43 min, in a separation medium of methanol/dichloromethane/triethylamine/acetic acid 60:40:1.08:0.36 (v/v/v/v). Both normal and reverse CE polarities could be applied for proper detection of the analytes owing to the combination of a suction effect caused by the nebulizer gas at the outlet end of the capillary and external pressure applied on the inlet vial. The separated lipid A species could be identified unequivocally by their characteristic fragmentation patterns through CID performed in both negative- and positive-ionization modes. The uniqueness of the NACE-ESI-MS/MS method lies in its simplicity and reliability for proving the phosphorylation isomerism (C1 or C4') and acylation pattern of native lipid A species or those designed for therapeutic applications. This article is protected by copyright. All rights reserved.}, keywords = {lipid A; acylation isomerism; nonaqueous capillary electrophoresis-tandem mass spectrometry; normal and reverse polarity; phosphorylation isomerism}, year = {2020}, eissn = {1522-2683}, pages = {1178-1188}, orcid-numbers = {Kilár, Ferenc/0000-0001-5737-5514; Dörnyei, Ágnes/0000-0002-6552-8522} } @{MTMT:30816329, title = {Comparison of LC-MS and CE MS for the analysis of immunstimulant bacterial membrane glycolipids}, url = {https://m2.mtmt.hu/api/publication/30816329}, author = {Sándor, Viktor and Kilár, Anikó and Berkics, Balázs Viktor and Kocsis, Béla and Kilár, Ferenc and Dörnyei, Ágnes}, booktitle = {12th Balaton Symposium on High-Performance Separation Methods}, unique-id = {30816329}, year = {2019}, pages = {105}, orcid-numbers = {Kilár, Ferenc/0000-0001-5737-5514; Dörnyei, Ágnes/0000-0002-6552-8522} } @article{MTMT:3360068, title = {Fast determination of lactic, succinic, malic, tartaric, shikimic and citric acids in red Vranec wines by CZE-ESI-QTOF-MS}, url = {https://m2.mtmt.hu/api/publication/3360068}, author = {Ivanova-Petropulos, V and Naceva, Z and Sándor, Viktor and Makszin, Lilla and Nagy, Laura and Berkics, Balázs Viktor and Stafilov, T and Kilár, Ferenc}, doi = {10.1002/elps.201700492}, journal-iso = {ELECTROPHORESIS}, journal = {ELECTROPHORESIS}, volume = {39}, unique-id = {3360068}, issn = {0173-0835}, abstract = {A fast and simple method with capillary zone electrophoresis coupled to electrospray ionization/quadrupole-time-of-flight-mass spectrometry (CZE-ESI/QTOF-MS) was optimized and validated for quantitative determination of organic acids (lactic acid, succinic acid, malic acid, tartaric acid, shikimic acid and citric acid) in red wines. The background electrolyte was ammonium acetate and the separation of the analytes was performed in a polybrene-coated capillary in the presence of electroosmotic flow. The sample preparation included dilution and filtration of the wine. The method showed satisfactory performance characteristics: good linearity for each organic acid, with correlation coefficients ranging from r(2) = 0.9902 (shikimic acid) to r(2) = 0.9990 (tartaric acid). The limit of quantification was between 0.0034 mM (for shikimic acid) and 0.107 mM (for citric acid), and the recovery data fell between 95.8 % (malic acid) and 102.7 % (lactic acid); the total run time was less than 4 minutes. The RSD values for the inter-day repeatability and intra-day reproducibility were between 3.44 and 9.50 %, and between 1.75 and 8.29 %, respectively. Seventeen Macedonian red Vranec wines were studied demonstrating a wide variation in the organic acids' concentration, which should be most probably due to the variation of the climate conditions in the vine areas. This article is protected by copyright. All rights reserved.}, year = {2018}, eissn = {1522-2683}, pages = {1597-1605}, orcid-numbers = {Makszin, Lilla/0000-0002-9764-4763; Kilár, Ferenc/0000-0001-5737-5514} } @article{MTMT:3276390, title = {Advanced on-line mass spectrometry detection of proteins separated by capillary isoelectric focusing after sequential injection}, url = {https://m2.mtmt.hu/api/publication/3276390}, author = {Páger, Csilla and Biherczová, Nikoleta and Ligetvári, Roland and Berkics, Balázs Viktor and Pongrácz, Tamás and Sándor, Viktor and Bufa, Anita and Poór, Viktória and Vojs Staňová, Andrea and Kilár, Ferenc}, doi = {10.1002/jssc.201700695}, journal-iso = {J SEP SCI}, journal = {JOURNAL OF SEPARATION SCIENCE}, volume = {40}, unique-id = {3276390}, issn = {1615-9306}, year = {2017}, eissn = {1615-9314}, pages = {4825-4834}, orcid-numbers = {Pongrácz, Tamás/0000-0002-8089-4352; Kilár, Ferenc/0000-0001-5737-5514} } @{MTMT:3139957, title = {Kapilláris izoelektromos fókuszálás alkalmazása tömegspektrometriás detektálással fehérjekeverék elválasztására}, url = {https://m2.mtmt.hu/api/publication/3139957}, author = {Páger, Csilla and Biherczova, Nikoleta and Ligetvári, Roland and Berkics, Balázs Viktor and Sándor, Viktor and Kilár, Ferenc}, booktitle = {Elválasztástudományi Vándorgyűlés 2016}, unique-id = {3139957}, year = {2016}, orcid-numbers = {Kilár, Ferenc/0000-0001-5737-5514} } @CONFERENCE{MTMT:3093098, title = {Determination of Organic Acids in Wines Using Capillary Zone Electrophoresis - Electrospray Ionization / Quadrupole - Time - of - Flight - Mass Spectrometry (CZE - ESI/QTOF - MS)}, url = {https://m2.mtmt.hu/api/publication/3093098}, author = {Violeta, Ivanova - Petropulos and Zaneta, Naceva and Sándor, Viktor and Berkics, Balázs Viktor and Trajce, Stafilov and Kilár, Ferenc}, booktitle = {16th CEEPUS Symposium and Summer School on Bioanalysis}, unique-id = {3093098}, year = {2016}, pages = {22}, orcid-numbers = {Kilár, Ferenc/0000-0001-5737-5514} } @CONFERENCE{MTMT:2775516, title = {Nitrofenol festékek és fehérjék elválasztása kapilláris izoelektromos fókuszálással, izoelektromos fókuszálás kapcsolása tömegspektrométerre}, url = {https://m2.mtmt.hu/api/publication/2775516}, author = {Berkics, Balázs Viktor and Pongrácz, Tamás and Poór, Viktória and Sándor, Viktor and Csóka, Balázs and Páger, Csilla and Kilár, Ferenc}, booktitle = {Elválasztástudományi Vándorgyűlés 2014}, unique-id = {2775516}, year = {2014}, pages = {79}, orcid-numbers = {Pongrácz, Tamás/0000-0002-8089-4352; Kilár, Ferenc/0000-0001-5737-5514} }