TY - CONF AU - Donát, Magyar AU - Tischner, Zsófia Bernadett AU - Anna, Páldy AU - Kocsubé, Sándor AU - Zsuzsanna, Dancsházy AU - Ágnes, Halász AU - Kredics, László TI - The impact of global megatrends on microfungi in the Pannonian Biogeographical Region: a climate change perspective T2 - II. Natural Hazards and Climate Change Conference: International Conference for identifying and tackling challenges together PY - 2025 SP - 11 EP - 11 PG - 1 UR - https://m2.mtmt.hu/api/publication/36176779 ID - 36176779 LA - English DB - MTMT ER - TY - CONF AU - Misz, András AU - Amanda, Sándorné Szőke AU - Bajzát, Judit AU - Dániel, Kökény AU - Marianna, Visnyei AU - Kredics, László AU - Allaga, Henrietta AU - Kocsubé, Sándor AU - Vágvölgyi, Csaba AU - Csutorás, Csaba ED - Gyalai, Ingrid ED - Czóbel, Szilárd TI - Development of casing materials used in white button mushroom cultivation by composting of spent mushroom compost T2 - 22nd Wellmann International Scientific Conference : Book of abstracts PB - University of Szeged Faculty of Agriculture C1 - Hódmezővásárhely SN - 9789636880446 PY - 2025 SP - 75 EP - 75 PG - 1 UR - https://m2.mtmt.hu/api/publication/36177019 ID - 36177019 LA - English DB - MTMT ER - TY - JOUR AU - Marik, Tamás AU - Gufu, Bonaya AU - Vishwanathula, Anusha AU - Balázs, Dóra Krisztina AU - Rozsnyói, Ákos AU - Terna, Gergő AU - Kovács, Fanni AU - Kocsubé, Sándor AU - Varga, Mónika AU - Szekeres, András AU - Druzhinina, Irina S. AU - Vágvölgyi, Csaba AU - Papp, Tamás AU - Tyagi, Chetna AU - Kredics, László TI - Novel peptaibiotics identified from Trichoderma clade Viride JF - NATURAL PRODUCTS AND BIOPROSPECTING J2 - NATURAL PRODUCTS AND BIOPROSPECTING VL - 15 PY - 2025 IS - 1 PG - 55 SN - 2192-2195 DO - 10.1007/s13659-025-00524-9 UR - https://m2.mtmt.hu/api/publication/36279134 ID - 36279134 AB - This study focuses on the peptaibiome produced by different species of Trichoderma belonging to clade Viride : T. koningii SZMC 28387 (CBS 979.70), T. cf . strigosellum SZMC 28007 (TUCIM 4886/IQ 191), T. cf . dorothopsis SZMC 28390 (TUCIM 416/TUB F-597), T. cf . strigosellum SZMC 28391 (TUCIM 423/DAOM 230018), T. atroviride SZMC 28748 (IMI 206040), T. hamatum SZMC 28747 (TUCIM 2730) and T. cf . dorothopsis SZMC 28005 (TUCIM 4882/IQ 11). We were able to identify new compounds with similarity to already known groups of peptaibiotics, as well as completely newly discovered compounds using high-performance liquid chromatography (HPLC) -mass spectrometry (MS). From the 367 peptaibiotics identified, 216 are peptaibols and 111 are lipopeptaibols. Out of all peptaibols, 55 are previously known, while 161 are newly discovered. The new peptaibol subgroups Strigosellin A, B and Dorothopsin A, B are introduced. Furthermore, besides 38 previously known lipopeptaibols, 73 new lipopeptaibol sequences, named Lipostrigosellins and Lipohamatins are also reported. In addition, 41 peptaibol-like compounds with unusual C-terminus were also found. Out of the 7 strains examined, 5 produced both peptaibols and lipopeptaibols, while 2 only peptaibols. The well-known compound, Trikoningin KA V (TRK-V) also produced by T. koningii SZMC 28387 (CBS 979.70), was studied for its folding dynamics using accelerated molecular dynamics simulations (aMD) for understanding the plausible three-dimensional structures adopted by these peptaibols of clade Viride . We observed a propensity to form kinked, right-handed helical structures when simulated in an aqueous environment. LA - English DB - MTMT ER - TY - JOUR AU - Ámon, Judit AU - Nemuuzaya, Suren AU - Alczheimer, Kevin AU - Kocsubé, Sándor AU - Farkas, Zoltán AU - Svorenj, Gergő AU - Gácser, Attila AU - Tyagi, Chetna AU - Kozma-Bognár, László AU - Kozma-Bognárné Hamari, Zsuzsanna TI - A non-canonical fungal peroxisome PTS-1 signal, SYM, and its evolutionary aspects JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 15 PY - 2025 IS - 1 PG - 15 SN - 2045-2322 DO - 10.1038/s41598-025-13871-x UR - https://m2.mtmt.hu/api/publication/36281577 ID - 36281577 N1 - Funding Agency and Grant Number: University of Szeged [DE-AC02-05CH11231]; DOE Office of Science [261572, 675858, 777536, 101017567, INFN-LNL-2, IN2P3-CPPM]; FP7 WeNMR; NIKHEF; US Open Science Grid; National Academy of Scientist Education Program of the National Biomedical Foundation under the sponsorship of the Hungarian Ministry of Culture and Innovation [AN-128740, K-134567]; National Research, Development and Innovation Office Funding text: JGI Sequences used in the SYM-protein analysis and pectinesterase localization analysis were from the US Department of Energy Joint Genome Institute in collaboration with the user community. We acknowledge the 1000 Fungal Genomes project consortium (PI's: F. Martin, R. Vilgalys, S.E. Baker, I.V. Grigoriev, J. Spatafora, M.R. Andersen, L-J. Ma, R. Proctor, R. Hamelin, G. Bonito, J.J. Coleman, H.C. Kistler, A. Eichmeier, A. Owensby Quandt, (A) Kohler, (B) Min, (C) Hori, C. Hittinger, C. Hansel, D.C. Eastwood, (D) Lindner, D. Raudabaugh, D. Catcheside, D. Salvachua, I-G. Choi, J.E. Stajich, J. Gladden, J. Vogel, J.W. Taylor, K. Peay, K. Solomon, L. Nagy, M.C. Aime, M. Kluge, M-N. Rosso, M. Campbell, M. Freitag, N. Nguyen, O. Yarden, P. Chain, P. Inderbitzin, P. Crous, P. Gladieux, P.E. Busby, S. Duplessis, S.W. Singer, T.D. Bruns, T.Y. James, T. Ward, and V. Subramanian) for providing access to unpublished genome data and permitting us to publish our analysis. The genome sequence data were conducted by the U.S. Department of Energy (DOE) Joint Genome Institute (http://www.jgi.doe.gov/) (https://ror.org/04xm1d337), a DOE Office of Science User Facility, was supported by the DOE Office of Science under contract number DE-AC02-05CH11231. he FP7 WeNMR (project# 261572), H2020 West-Life (project# 675858), the EOSC-hub (project# 777536) and the EGI-ACE (project# 101017567) European e-Infrastructure projects are acknowledged for the use of their web portals, which make use of the EGI infrastructure with the dedicated support of CESNET-MCC, INFN-LNL-2, NCG-INGRID-PT, TW-NCHC, IFCA-LCG2, UA-BITP, TR-FC1-ULAKBIM, CSTCLOUD-EGI, IN2P3-CPPM, SURFsara and NIKHEF, and the additional support of the national GRID Initiatives of Belgium, France, Italy, Germany, the Netherlands, Poland, Portugal, Spain, UK, Taiwan and the US Open Science Grid. Research work of AG and GS were conducted with the support of the National Academy of Scientist Education Program of the National Biomedical Foundation under the sponsorship of the Hungarian Ministry of Culture and Innovation. LKB's work was supported by the National Research, Development and Innovation Office (grant numbers AN-128740 and K-134567). AB - Proteins localized to peroxisomes, particularly those expressed under specific conditions or in low abundance, are often undetected by routine proteomics methods due to detection sensitivity limits. In silico identification and experimental validation of peroxisomal targeting signals (PTSs) offer a reliable alternative. We demonstrate that SYM, a non-canonical plant PTS-1 signal, functions similarly in Aspergillus nidulans , as GFP tagged with a SYM C-terminal tripeptide localizes to peroxisomes. One of two native A. nidulans proteins with C-terminal SYM tripeptide shows weak peroxisomal localization alongside cytoplasmic presence, indicating that only a subset of proteins with non-canonical signals access peroxisomes. In silico analysis of 1,010 fungal genomes identified diverse SYM-proteins with variable functions, suggesting that non-canonical PTS-1 signals may evolve spontaneously. Two-thirds of SYM-proteins are predicted to localize to specific intracellular compartments other than the peroxisome. We propose that despite their predicted localization, these proteins possessing SYM as a non-canonical peroxisomal signal might also have peroxisomal presence. Among SYM-proteins, pectinesterases, known plant pathogen virulence factors, were frequent. Notably, 25% of fungal pectinesterases harbor non-canonical PTS-1 signals, suggesting that partial peroxisomal localization of pectinesterases has evolved convergently. This suggests that partial peroxisomal localization may enhance protein functional flexibility, contributing to the organism’s adaptability. LA - English DB - MTMT ER - TY - JOUR AU - Vágó, Bernadett AU - Bauer, Kitti AU - Varghese, Naomi AU - Kiss-Vetráb, Sándor AU - Kocsubé, Sándor AU - Varga, Mónika AU - Szekeres, András AU - Vágvölgyi, Csaba AU - Papp, Tamás AU - Nagy, Gábor TI - Role of the putative sit1 gene in normal germination of spores and virulence of the Mucor lusitanicus JF - MICROBIAL CELL J2 - MICROBIAL CELL VL - 12 PY - 2025 IS - 1 SP - 195 EP - 209 PG - 15 SN - 2311-2638 DO - 10.15698/mic2025.08.856 UR - https://m2.mtmt.hu/api/publication/36297827 ID - 36297827 AB - Mucormycosis is a life-threatening infection caused by certain members of the fungal order Mucorales, with increased incidence in recent years. Individuals with untreated diabetes mellitus, and patients treated with deferoxamine are particularly susceptible to this infection. Elevated free iron concentrations in serum contribute to the development of mucormycosis. Pathogenic fungi have evolved multiple mechanisms to acquire and utilize free iron or extract it from the various iron-binding molecules within the host. The utilization of hydroxamate siderophores as xenosiderophores may contribute to the development of mucormycosis. The genome of Mucor lusitanicus encodes one Sit1 siderophore transporter. In this study, the role of the sit1 gene was characterized by generating knockout mutants using CRISPR-Cas9. Relative transcript level of the sit1 gene significantly increased in the presence of deferoxamine- and deferasirox-iron complexes. Lack of sit1 resulted in altered germination of spores and growth ability, and decreased virulence. Furthermore, absence of the gene caused elevated transcript levels of a ferric reductase (FRE), a low-affinity iron permease (FET4) and a copper dependent iron oxidase (FET3). Our result suggests that expressions of the genes involved in iron uptake affect each other. The lack of Sit1 resulted in an increased transcript level of the FRE3 gene, which may be able to reduce iron from the siderophore-iron complex. The reduced and liberated iron may be then taken up by activated FET4a. This study highlights the significance of understanding the iron acquisition mechanisms of pathogenic fungi to develop effective treatments for fungal infections. LA - English DB - MTMT ER - TY - JOUR AU - Absharina, Dheanda AU - Putra, Filemon Jalu Nusantara AU - Ogino, Chiaki AU - Kocsubé, Sándor AU - Veres, Csilla AU - Vágvölgyi, Csaba TI - Bacterial Cellulose Production in Co-Culture Systems: Opportunities, Challenges, and Future Directions JF - APPLIED MICROBIOLOGY J2 - APPL MICROBIOL VL - 5 PY - 2025 IS - 3 PG - 16 SN - 2673-8007 DO - 10.3390/applmicrobiol5030092 UR - https://m2.mtmt.hu/api/publication/36328243 ID - 36328243 AB - Bacterial cellulose (BC), a nanostructured biopolymer produced by Komagateibacter spp., exhibits remarkable mechanical strength, purity, and biocompatibility, making it highly attractive for applications in biomedicine, food, and sustainable materials. Despite its potential, monoculture fermentation suffers from low yield and limited scalability. This review highlights the innovative application of co-culture fermentations as a novel strategy, where Komagataeibacter is paired with complementary microorganisms such as yeasts, lactic acid bacteria, and photosynthetic microbes. This approach has emerged as a promising solution to overcome the limitations of monoculture by enhancing BC productivity, tailoring material properties, and improving sustainability. We explore the synergistic interactions within co-cultures, including metabolic cross-feeding and in situ polymer integration, while also addressing critical challenges such as microbial stability and operational complexity. Unlike previous reviews focused primarily on BC biosynthesis, applications, or genetic engineering, this article emphasizes co-culture fermentation with Komagataeibacter as a novel and underexplored strategy to improve the yield, functionality, and scalability of BC production. LA - English DB - MTMT ER - TY - CONF AU - Szebenyi, Csilla AU - Kocsubé, Sándor AU - Molnár, Anna AU - Kiss, Karina AU - Sinka, Rita AU - Németh, Dóra AU - Szegedi, Botond Ferenc AU - Abu Saleem, Tammam Khaliefeh Siliman AU - Vágvölgyi, Csaba AU - Papp, Tamás TI - Virulence-associated traits in Mucor lusitanicus T2 - ABSTRACTS of the 7th Central European Forum for Microbiology PY - 2025 SP - 74 EP - 74 PG - 1 UR - https://m2.mtmt.hu/api/publication/36410534 ID - 36410534 LA - English DB - MTMT ER - TY - JOUR AU - Flipphi, Michel Johannes Anthonie AU - Harispe, María Laura AU - Kozma-Bognárné Hamari, Zsuzsanna AU - Kocsubé, Sándor AU - Scazzocchio, Claudio AU - Ramón, Ana TI - An ascomycete H4 variant with an unknown function JF - ROYAL SOCIETY OPEN SCIENCE J2 - R SOC OPEN SCI VL - 11 PY - 2024 IS - 2 PG - 16 SN - 2054-5703 DO - 10.1098/rsos.231705 UR - https://m2.mtmt.hu/api/publication/34682151 ID - 34682151 AB - Histone variants leading to altered nucleosome structure, dynamics and DNA accessibility occur frequently, albeit rarely for H4. We carried out a comprehensive in silico scrutiny of fungal genomes, which revealed the presence of a novel H4 variant (H4E) in the ascomycetes, throughout the Pezizomycotina, in basal species of the Taphrinomycotina and also in the Glomeromycota. The coding cognate genes show a specific intron/exon organization, different from H4 canonical genes. H4Es diverge from canonical H4s mainly in the N- and C-terminal extensions, showing marked differences in the distribution and number of Lys and Arg residues, which may result in novel post-translational modifications. In Aspergillus nidulans (Pezizomycotina, Eurotiomycetes) the H4E variant protein level is low in mycelia. However, the encoding gene is well expressed at 37°C under nitrogen starvation. H4E localizes to the nucleus and interacts with H3, but its absence or overexpression does not result in any detectable phenotype. Deletion of only one of the of the two canonical H4 genes results in a strikingly impaired growth phenotype, which indicates that H4E cannot replace this canonical histone. Thus, an H4 variant is present throughout a whole subphylum of the ascomycetes, but with hitherto no experimentally detectable function. LA - English DB - MTMT ER - TY - CONF AU - Vágó, Bernadett AU - Bauer, Kitti AU - Kiss-Vetráb, Sándor AU - Kocsubé, Sándor AU - Vágvölgyi, Csaba AU - Papp, Tamás AU - Nagy, Gábor ED - Balázs, Dóra Krisztina ED - Rozsnyói, Ákos TI - A Sit1 sziderofór transzporter szerepe Mucor lusitanicusban T2 - Biotechnológiai Szakmai Nap Absztraktfüzet PB - Doktoranduszok Országos Szövetsége (DOSZ) C1 - Budapest SN - 9786156457448 PY - 2024 UR - https://m2.mtmt.hu/api/publication/34723140 ID - 34723140 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Visagie, C. M. AU - Yilmaz, N. AU - Kocsubé, Sándor AU - Frisvad, J. C. AU - Hubka, V. AU - Samson, R. A. AU - Houbraken, J. TI - A review of recently introduced Aspergillus, Penicillium, Talaromyces and other Eurotiales species JF - STUDIES IN MYCOLOGY J2 - STUD MYCOL VL - 107 PY - 2024 IS - 1 SP - 1 EP - 66 PG - 66 SN - 0166-0616 DO - 10.3114/sim.2024.107.01 UR - https://m2.mtmt.hu/api/publication/34747961 ID - 34747961 N1 - Funding Agency and Grant Number: European Union's Horizon 2020 research and innovation program (RISE) under the Marie Sklodowska-Curie grant [101008129]; University of Pretoria's University Capacity Development Programme (UCDP) grant; Czech Ministry of Health [NU21-05-00681] Funding text: C.M. Visagie and N. Yilmaz received funding from the European Union's Horizon 2020 research and innovation program (RISE) under the Marie Sklodowska-Curie grant agreement No. 101008129, project acronym "Mycobiomics". C.M. Visagie was also supported by the University of Pretoria's University Capacity Development Programme (UCDP) grant. V. Hubka was supported by the Czech Ministry of Health (grant NU21-05-00681) . We are grateful to K. Bensch for her advice and guidance, and we thank L. Krieglsteiner (Spraitbach, Germany) and D. Malloch for sharing specimens of Aspergillus lentisci . AB - The order Eurotiales is diverse and includes species that impact our daily lives in many ways. In the past, its taxonomy was difficult due to morphological similarities, which made accurate identification of species difficult. This situation improved and stabilised with recent taxonomic and nomenclatural revisions that modernised Aspergillus, Penicillium and Talaromyces. This was mainly due to the availability of curated accepted species lists and the publication of comprehensive DNA sequence reference datasets. This has also led to a sharp increase in the number of new species described each year with the accepted species lists in turn also needing regular updates. The focus of this study was to review the 160 species described between the last list of accepted species published in 2020 until 31 December 2022. To review these species, single-gene phylogenies were constructed and GCPSR (Genealogical Concordance Phylogenetic Species Recognition) was applied. Multi-gene phylogenetic analyses were performed to further determine the relationships of the newly introduced species. As a result, we accepted 133 species (37 Aspergillus, two Paecilomyces, 59 Penicilium, two Rasamsonia, 32 Talaromyces and one Xerochrysium), synonymised 22, classified four as doubtful and created a new combination for Paraxerochrysium coryli, which is classified in Xerochrysium. This brings the number of accepted species to 453 for Aspergillus, 12 for Paecilomyces, 535 for Penicillium, 14 for Rasamsonia, 203 for Talaromyces and four for Xerochrysium. We accept the newly introduced section Tenues (in Talaromyces), and series Hainanici (in Aspergillus sect. Cavernicolarum) and Vascosobrinhoana (in Penicillium sect. Citrina). In addition, we validate the invalidly described species Aspergillus annui and A. saccharicola, and series Annuorum (in Aspergillus sect. Flavi), introduce a new combination for Dichlaena lentisci (type of the genus) and place it in a new section in Aspergillus subgenus Circumdati, provide an updated description for Rasamsonia oblata, and list excluded and recently synonymised species that were previously accepted. This study represents an important update of the accepted species lists in Eurotiales. LA - English DB - MTMT ER -