TY - JOUR AU - Gálosi, Rita AU - Szalay, László Csaba AU - Aradi, Mihály AU - Perlaki, Gábor AU - Pál, József AU - Steier, Roy AU - Lénárd, László AU - Karádi, Zoltán György TI - Identifying non-toxic doses of manganese for manganese-enhanced magnetic resonance imaging to map brain areas activated by operant behavior in trained rats. JF - MAGNETIC RESONANCE IMAGING J2 - MAGN RESON IMAGING VL - 37 PY - 2017 SP - 122 EP - 133 PG - 12 SN - 0730-725X DO - 10.1016/j.mri.2016.11.017 UR - https://m2.mtmt.hu/api/publication/3148677 ID - 3148677 N1 - Case report Funding Agency and Grant Number: PTE [AOK-KA 2013/34039/1]; [SROP-4.2.2/B-10/1-2010-0029]; [SCROP-4.2.1.B-10/2/KONV-2010-002] Funding text: Authors wish to thank Attila Schwartz for his technical support. We would like to thank Mrs. Iren Takacs, Mrs. Erzsebet Kovacs and Ildiko Fuchs for their technical assistance. This study was supported by SROP-4.2.2/B-10/1-2010-0029, SCROP-4.2.1.B-10/2/KONV-2010-002 and by PTE AOK-KA 2013/34039/1. AB - Manganese-enhanced magnetic resonance imaging (MEMRI) offers unique advantages such as studying brain activation in freely moving rats, but its usefulness has not been previously evaluated during operant behavior training. Manganese in a form of MnCl2, at a dose of 20mg/kg, was intraperitoneally infused. The administration was repeated and separated by 24h to reach the dose of 40mg/kg or 60mg/kg, respectively. Hepatotoxicity of the MnCl2 was evaluated by determining serum aspartate aminotransferase, alanine aminotransferase, total bilirubin, albumin and protein levels. Neurological examination was also carried out. The animals were tested in visual cue discriminated operant task. Imaging was performed using a 3T clinical MR scanner. T1 values were determined before and after MnCl2 administrations. Manganese-enhanced images of each animal were subtracted from their baseline images to calculate decrease in the T1 value (DeltaT1) voxel by voxel. The subtracted T1 maps of trained animals performing visual cue discriminated operant task, and those of naive rats were compared. The dose of 60mg/kg MnCl2 showed hepatotoxic effect, but even these animals did not exhibit neurological symptoms. The dose of 20 and 40mg/kg MnCl2 increased the number of omissions and did not affect the accuracy of performing the visual cue discriminated operant task. Using the accumulated dose of 40mg/kg, voxels with a significant enhanced DeltaT1 value were detected in the following brain areas of the visual cue discriminated operant behavior performed animals compared to those in the controls: the visual, somatosensory, motor and premotor cortices, the insula, cingulate, ectorhinal, entorhinal, perirhinal and piriform cortices, hippocampus, amygdala with amygdalohippocampal areas, dorsal striatum, nucleus accumbens core, substantia nigra, and retrorubral field. In conclusion, the MEMRI proved to be a reliable method to accomplish brain activity mapping in correlation with the operant behavior of freely moving rodents. LA - English DB - MTMT ER - TY - THES AU - Steier, Roy TI - Investigation of the hyponatremic brain edema, and the involvement of epithelial sodium channels in its evolution PY - 2012 SP - 65 UR - https://m2.mtmt.hu/api/publication/2241173 ID - 2241173 LA - English DB - MTMT ER - TY - JOUR AU - Steier, Roy AU - Aradi, Mihály AU - Pál, József AU - Perlaki, Gábor AU - Orsi, Gergely AU - Bogner, Péter AU - Gallyas, Ferenc AU - Bukovics, Péter AU - Janszky, József Vladimír AU - Dóczi, Tamás Péter AU - Schwarcz, Attila TI - A biexponential DWI study in rat brain intracellular oedema. JF - EUROPEAN JOURNAL OF RADIOLOGY J2 - EUR J RADIOL VL - 81 PY - 2012 IS - 8 SP - 1758 EP - 1765 PG - 8 SN - 0720-048X DO - 10.1016/j.ejrad.2011.03.058 UR - https://m2.mtmt.hu/api/publication/1619045 ID - 1619045 N1 - Department of Neurosurgery, Faculty of Medicine University of Pécs, Rét street 2, H-7623 Pécs, Hungary Pécs Diagnostic Center, Faculty of Medicine University of Pécs, Rét street 2, H-7623 Pécs, Hungary Department of Neurology, Faculty of Medicine University of Pécs, Rét street 2, H-7623 Pécs, Hungary Cited By :6 Export Date: 27 February 2024 CODEN: EJRAD Correspondence Address: Steier, R.; Department of Neurosurgery, Rét street 2, H-7623 Pécs, Hungary; email: roy.steier@gmail.com LA - English DB - MTMT ER - TY - CONF AU - Szalay, László Csaba AU - Gálosi, Rita AU - Aradi, Mihály AU - Steier, Roy AU - Perlaki, Gábor AU - Orsi, Gergely AU - Schwarcz, Attila AU - Lénárd, László AU - Karádi, Zoltán György TI - Mapping whole-brain activity in behaving rats using activity-induced manganese-enhanced MRI in a 3T clinical scanner. T2 - 13th Conference of the Hungarian Neuroscience Society (MITT) PY - 2011 UR - https://m2.mtmt.hu/api/publication/1530249 ID - 1530249 LA - English DB - MTMT ER - TY - JOUR AU - Steier, Roy AU - Aradi, Mihály AU - Pál, József AU - Sulyok, Endre AU - Perlaki, Gábor AU - Orsi, Gergely AU - Janszky, József Vladimír AU - Schwarcz, Attila AU - Dóczi, Tamás Péter TI - The influence of benzamil hydrochloride on the evolution of hyponatremic brain edema as assessed by in vivo MRI study in rats. JF - ACTA NEUROCHIRURGICA J2 - ACTA NEUROCHIR VL - 153 PY - 2011 IS - 10 SP - 2091 EP - 2097 PG - 7 SN - 0001-6268 DO - 10.1007/s00701-011-0996-3 UR - https://m2.mtmt.hu/api/publication/1474159 ID - 1474159 N1 - Funding Agency and Grant Number: Marie Curie Research Training Networks Aquaglyceroporins [MRTN-CT-2006-035995]; Hungarian Science Founds (OTKA) [PD 72240]; Hungarian Academy of Science Funding text: This study was supported by the following grants: Marie Curie Research Training Networks Aquaglyceroporins (MRTN-CT-2006-035995); Hungarian Science Founds (OTKA PD 72240); "Save what can be saved"-Applied neurological research using high-field magnetic resonance imaging HU0114; "Visible pain"-New fMRI methods in pain research and treatment. S.A. and J.J. were supported by Bolyai fellowship of the Hungarian Academy of Science. LA - English DB - MTMT ER - TY - JOUR AU - Aradi, Mihály AU - Steier, Roy AU - Bukovics, Péter AU - Szalay, László Csaba AU - Perlaki, Gábor AU - Orsi, Gergely AU - Pál, József AU - Janszky, József Vladimír AU - Dóczi, Tamás Péter AU - Schwarcz, Attila TI - Quantitative proton MRI and MRS of the rat brain with a 3T clinical MR scanner. JF - JOURNAL OF NEURORADIOLOGY J2 - J NEURORADIOLOGY VL - 38 PY - 2011 IS - 2 SP - 90 EP - 97 PG - 8 SN - 0150-9861 DO - 10.1016/j.neurad.2009.11.003 UR - https://m2.mtmt.hu/api/publication/1423337 ID - 1423337 N1 - Department of Neurosurgery, University of Pécs, Rét u. 2, 7624 Pécs, Hungary Pécs Diagnostic Institute, University of Pécs, 7624 Pécs, Hungary Institute of Physiology, University of Pécs, 7624 Pécs, Hungary Department of Neurology, University of Pécs, 7624 Pécs, Hungary Cited By :10 Export Date: 27 February 2024 CODEN: JNEUD Correspondence Address: Aradi, M.; Department of Neurosurgery, Rét u. 2, 7624 Pécs, Hungary; email: postmaster@aradimis.com AB - OBJECTIVE: To demonstrate the capability of a clinical 3T human scanner in performing quantitative MR experiments in the rat brain. MATERIAL AND METHODS: In vivo, measurements on eight Wistar rats were performed. Longitudinal relaxation time (T(1)) and transverse relaxation time (T(2)) measurements were set up at a spatial resolution of 0.3x0.3x1mm(3). Diffusion-weighted imaging was also applied and the evaluation included both mono- and biexponential approaches (b-value up to 6000s/mm(2)). Besides quantitative imaging, the rat brain was also scanned at a microscopic resolution of 130x130x130mum(3). Quantitative proton spectroscopy was also carried out on the rat brain with water as internal reference. RESULTS: T(1) and T(2) for the rat brain cortex were 1272+/-85ms and 75+/-2ms, respectively. Diffusion-weighted imaging yielded accurate diffusion coefficient measurements at both low and high b-value ranges. The concentrations of MR visible metabolites were determined for the major resonances (i.e., N-acetyl-aspartate, choline and creatine) with acceptable accuracy. CONCLUSION: The results suggest that quantitative imaging and spectroscopy can be carried out on small animals on high-field clinical scanners. LA - English DB - MTMT ER - TY - JOUR AU - Mester, L AU - Szabadfi, Krisztina AU - Reglődi, Dóra AU - Aradi, M AU - Steier, Roy AU - Schwarcz, Attila AU - Dóczi, Tamás Péter AU - Kiss, Péter AU - Rácz, Boglárka AU - Kovács, Krisztina AU - Szabó, Aliz AU - Tamás, Andrea AU - Gábriel, Róbert AU - Atlasz, Tamás TI - Neuroprotective strategies in ischemic retinal lesions. JF - IDEGGYOGYASZATI SZEMLE / CLINICAL NEUROSCIENCE J2 - IDEGGYOGY SZEMLE VL - 63 PY - 2010 IS - 5-6 SP - 206 SN - 0019-1442 UR - https://m2.mtmt.hu/api/publication/1740488 ID - 1740488 LA - English DB - MTMT ER - TY - JOUR AU - Sulyok, Endre AU - Pál, József AU - Vajda, Zsolt AU - Steier, Roy AU - Dóczi, Tamás Péter TI - Benzamil prevents brain water accumulation in hyponatraemic rats. JF - ACTA NEUROCHIRURGICA J2 - ACTA NEUROCHIR VL - 151 PY - 2009 IS - 9 SP - 1121 EP - 1125 PG - 5 SN - 0001-6268 DO - 10.1007/s00701-009-0354-x UR - https://m2.mtmt.hu/api/publication/1368307 ID - 1368307 N1 - Funding Agency and Grant Number: Hungarian Ministry of Health (ES) [50035]; European Human Resources and Mobility (HRM); Marie Curie Research Training Networks (RTN) Funding text: The study was supported by the Hungarian Ministry of Health (ES) by grant no. ETT 50035 and by the European Human Resources and Mobility (HRM), Marie Curie Research Training Networks (RTN) "Aqua(Glyecero)porin" project. LA - English DB - MTMT ER -