TY - CONF AU - Igaz, Nóra AU - Szőke, Krisztina AU - Bocz, Csenge AU - Kovács, Dávid AU - Rónavári, Andrea AU - Szabó, Emilia Rita AU - Polanek, Róbert AU - Buhala, Andrea AU - Vizler, Csaba AU - Tiszlavicz, László AU - Rázga, Zsolt AU - Hideghéty, Katalin AU - Kónya, Zoltán AU - Csontné Kiricsi, Mónika TI - Radiosensitizing effect of metal nanoparticles in combination with histone deacetylase inhibitors T2 - FAMÉ 2023 PY - 2023 SP - 75 EP - 76 PG - 2 UR - https://m2.mtmt.hu/api/publication/34154565 ID - 34154565 LA - English DB - MTMT ER - TY - JOUR AU - Asperger, Hannah AU - Stamm, Nadia AU - Gierke, Berthold AU - Pawlak, Michael AU - Hofmann, Ute AU - Zanger, Ulrich M. AU - Marton, Annamária AU - Katona, Róbert László AU - Buhala, Andrea AU - Vizler, Csaba AU - Cieslik, Jan-Philipp AU - Kovacevic, Zaklina AU - Richardson, Des R. AU - Ruckhaeberle, Eugen AU - Niederacher, Dieter AU - Fehm, Tanja AU - Neubauer, Hans AU - Ludescher, Marina TI - Progesterone receptor membrane component 1 regulates lipid homeostasis and drives oncogenic signaling resulting in breast cancer progression (vol 22, 75, 2020) JF - BREAST CANCER RESEARCH J2 - BREAST CANCER RES VL - 23 PY - 2021 IS - 1 SN - 1465-5411 DO - 10.1186/s13058-020-01383-7 UR - https://m2.mtmt.hu/api/publication/31873459 ID - 31873459 AB - An amendment to this paper has been published and can be accessed via the original article. LA - English DB - MTMT ER - TY - JOUR AU - Asperger, Hannah AU - Stamm, Nadia AU - Gierke, Berthold AU - Pawlak, Michael AU - Hofmann, Ute AU - Zanger, Ulrich M. AU - Marton, Annamária AU - Katona, Róbert László AU - Buhala, Andrea AU - Vizler, Csaba AU - Cieslik, Jan-Philipp AU - Ruckhaeberle, Eugen AU - Niederacher, Dieter AU - Fehm, Tanja AU - Neubauer, Hans AU - Ludescher, Marina TI - Progesterone receptor membrane component 1 regulates lipid homeostasis and drives oncogenic signaling resulting in breast cancer progression JF - BREAST CANCER RESEARCH J2 - BREAST CANCER RES VL - 22 PY - 2020 IS - 1 SN - 1465-5411 DO - 10.1186/s13058-020-01312-8 UR - https://m2.mtmt.hu/api/publication/31397452 ID - 31397452 AB - Background: PGRMC1 (progesterone receptor membrane component 1) is a highly conserved heme binding protein, which is overexpressed especially in hormone receptor-positive breast cancer and plays an important role in breast carcinogenesis. Nevertheless, little is known about the mechanisms by which PGRMC1 drives tumor progression. The aim of our study was to investigate the involvement of PGRMC1 in cholesterol metabolism to detect new mechanisms by which PGRMC1 can increase lipid metabolism and alter cancer-related signaling pathways leading to breast cancer progression. Methods: The effect of PGRMC1 overexpression and silencing on cellular proliferation was examined in vitro and in a xenograft mouse model. Next, we investigated the interaction of PGRMC1 with enzymes involved in the cholesterol synthesis pathway such as CYP51, FDFT1, and SCD1. Further, the impact of PGRMC1 expression on lipid levels and expression of enzymes involved in lipid homeostasis was examined. Additionally, we assessed the role of PGRMC1 in key cancer-related signaling pathways including EGFR/HER2 and ER alpha signaling. Results: Overexpression of PGRMC1 resulted in significantly enhanced proliferation. PGRMC1 interacted with key enzymes of the cholesterol synthesis pathway, alters the expression of proteins, and results in increased lipid levels. PGRMC1 also influenced lipid raft formation leading to altered expression of growth receptors in membranes of breast cancer cells. Analysis of activation of proteins revealed facilitated ER alpha and EGFR activation and downstream signaling dependent on PGRMC1 overexpression in hormone receptor-positive breast cancer cells. Depletion of cholesterol and fatty acids induced by statins reversed this growth benefit. Conclusion: PGRMC1 may mediate proliferation and progression of breast cancer cells potentially by altering lipid metabolism and by activating key oncogenic signaling pathways, such as ER alpha expression and activation, as well as EGFR signaling. Our present study underlines the potential of PGRMC1 as a target for anti-cancer therapy. LA - English DB - MTMT ER - TY - JOUR AU - Igaz, Nóra AU - Szőke, Krisztina AU - Kovács, Dávid AU - Buhala, Andrea AU - Varga, Zoltán AU - Bélteky, Péter AU - Rázga, Zsolt AU - Tiszlavicz, László AU - Vizler, Csaba AU - Hideghéty, Katalin AU - Kónya, Zoltán AU - Csontné Kiricsi, Mónika TI - Synergistic Radiosensitization by Gold Nanoparticles and the Histone Deacetylase Inhibitor SAHA in 2D and 3D Cancer Cell Cultures JF - NANOMATERIALS J2 - NANOMATERIALS-BASEL VL - 10 PY - 2020 IS - 1 PG - 20 SN - 2079-4991 DO - 10.3390/nano10010158 UR - https://m2.mtmt.hu/api/publication/31130654 ID - 31130654 LA - English DB - MTMT ER - TY - JOUR AU - Naziroglu, M AU - Cig, B AU - Blum, W AU - Vizler, Csaba AU - Buhala, Andrea AU - Marton, Annamária AU - Katona, Róbert László AU - Jósvay, Katalin AU - Schwaller, B AU - Oláh, Zoltán AU - Pecze, László TI - Targeting breast cancer cells by MRS1477, a positive allosteric modulator of TRPV1 channels JF - PLOS ONE J2 - PLOS ONE VL - 12 PY - 2017 IS - 6 PG - 19 SN - 1932-6203 DO - 10.1371/journal.pone.0179950 UR - https://m2.mtmt.hu/api/publication/3250677 ID - 3250677 N1 - OA gold AB - There is convincing epidemiological and experimental evidence that capsaicin, a potent natural transient receptor potential cation channel vanilloid member 1 (TRPV1) agonist, has anticancer activity. However, capsaicin cannot be given systemically in large doses, because of its induction of acute pain and neurological inflammation. MRS1477, a dihydropyridine derivative acts as a positive allosteric modulator of TRPV1, if added together with capsaicin, but is ineffective, if given alone. Addition of MRS1477 evoked Ca2+ signals in MCF7 breast cancer cells, but not in primary breast epithelial cells. This indicates that MCF7 cells not only express functional TRPV1 channels, but also produce endogenous TRPV1 agonists. We investigated the effects of MRS1477 and capsaicin on cell viability, caspase-3 and -9 activities and reactive oxygen species production in MCF7 cells. The fraction of apoptotic cells was increased after 3 days incubation with capsaicin (10 mu M) paralleled by increased reactive oxygen species production and caspase activity. These effects were even more pronounced, when cells were incubated with MRS1477 (2 mu M) either alone or together with CAPS (10 mu M). Capsazepine, a TRPV1 blocker, inhibited both the effect of capsaicin and MRS1477. Whole-cell patch clamp recordings revealed that capsaicinevoked TRPV1-mediated current density levels were increased after 3 days incubation with MRS1477 (2 mu M). However, the tumor growth in MCF7 tumor-bearing immunodeficient mice was not significantly decreased after treatment with MRS1477 (10 mg/kg body weight, i.p., injection twice a week). In conclusion, in view of a putative in vivo treatment with MRS1477 or similar compounds further optimization is required. LA - English DB - MTMT ER - TY - JOUR AU - Jósvay, Katalin AU - Winter, Zoltán AU - Katona, Róbert László AU - Pecze, László AU - Marton, Annamária AU - Buhala, Andrea AU - Szakonyi, Gerda AU - Oláh, Zoltán AU - Vizler, Csaba TI - Besides neuro-imaging, the Thy1-YFP mouse could serve for visualizing experimental tumours, inflammation and wound-healing JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 4 PY - 2014 SP - 6776 PG - 7 SN - 2045-2322 DO - 10.1038/srep06776 UR - https://m2.mtmt.hu/api/publication/2763894 ID - 2763894 AB - The B6.Cg-Tg(Thy1-YFP)16Jrs/J transgenic mouse strain, widely used to study neuronal development and regeneration, expresses the yellow fluorescent protein (YFP) in the peripheral nerves and the central nervous system under the control of regulatory sequences of the Thy1 gene. The Thy1 (CD90) cell surface glycoprotein is present on many cell types besides neurons, and is known to be involved in cell adhesion, migration and signal transduction. We hypothesized that Thy1-activating conditions could probably activate the truncated Thy1 regulatory sequences used in the Thy1-YFP construct, resulting in YFP transgene expression outside the nervous system. We demonstrated that the stroma of subcutaneous tumours induced by the injection of 4T1 or MC26 carcinoma cells into BALB/c(Thy1-YFP) mice, carrying the same construct, indeed expressed the YFP transgene. In the tumour mass, the yellow-green fluorescent stromal cells were clearly distinguishable from 4T1 carcinoma cells stably transfected with red fluorescent protein. Local inflammation induced by subcutaneous injection of complete Freund's adjuvant, as well as the experimental wound-healing milieu, also triggered YFP fluorescence in both the BALB/c(Thy1-YFP) and B6.Cg-Tg(Thy1-YFP)16Jrs/J mice, pointing to eventual overlapping pathways of wound-healing, inflammation and tumour growth. LA - English DB - MTMT ER - TY - JOUR AU - Winter, Zoltán AU - Buhala, Andrea AU - Ötvös, Ferenc AU - Jósvay, Katalin AU - Vizler, Csaba AU - Dombi, György AU - Szakonyi, Gerda AU - Oláh, Zoltán TI - Functionally important amino acid residues in the transient receptor potential vanilloid 1 (TRPV1) ion channel – an overview of the current mutational data JF - MOLECULAR PAIN J2 - MOL PAIN VL - 9 PY - 2013 IS - 1 PG - 29 SN - 1744-8069 DO - 10.1186/1744-8069-9-30 UR - https://m2.mtmt.hu/api/publication/2341377 ID - 2341377 LA - English DB - MTMT ER -