@inproceedings{MTMT:34160396, title = {A lítium-klorid kontakt hatásának igazolása in vitro és in situ Varroa atka elleni alkalmazása esetén}, url = {https://m2.mtmt.hu/api/publication/34160396}, author = {Kolics-Horváth, Éva and Mátyás, Kinga Klára and Taller, János and Specziár, András and Kolics, Balázs}, booktitle = {XXIX. Ifjúsági Tudományos Fórum : Konferenciakötet}, unique-id = {34160396}, year = {2023}, pages = {185-190} } @article{MTMT:34027121, title = {Méhészeti melléktermékek alkalmazása haltakarmányban a körforgásos gazdálkodás elősegítése érdekében}, url = {https://m2.mtmt.hu/api/publication/34027121}, isbn = {9789636230555}, author = {Vannaphar, Tamajedy and Nguyen, Kim Ngan and Jakabné Sándor, Zsuzsanna and Gyalog, Gergő and Kolics, Balázs and Kucska, Balázs}, journal-iso = {HALÁSZATFEJLESZTÉS}, journal = {HALÁSZATFEJLESZTÉS}, volume = {40}, unique-id = {34027121}, issn = {1219-4816}, year = {2023}, pages = {37-40}, orcid-numbers = {Jakabné Sándor, Zsuzsanna/0000-0002-5216-6964; Gyalog, Gergő/0000-0002-3778-203X} } @article{MTMT:33594577, title = {Efficacy of In Vitro Lithium Chloride Treatments on Dermacentor reticulatus}, url = {https://m2.mtmt.hu/api/publication/33594577}, author = {Kolics, Balázs and Mátyás, Kinga Klára and Solti, Izabella and Bacsi, Zsuzsanna and Kovács, Szilvia and Specziár, András and Taller, János and Kolics-Horváth, Éva}, doi = {10.3390/insects14020110}, journal-iso = {INSECTS}, journal = {INSECTS}, volume = {14}, unique-id = {33594577}, abstract = {Dermacentor reticulatus (Fabr., 1794) (Acari: Ixodidae) is parasite that spreads many diseases which are dangerous to humans and animals. Microelement lithium was found to have promising potential against the detrimental bee pest Varroa destructor. Furthermore, its effectiveness was confirmed against Dermanyssus gallinae, a major parasite of poultry, in vitro. In the present study, we investigated whether the efficacy of lithium chloride extends to other parasitic species, such as D. reticulatus. Our results revealed, for the first time, that the effectiveness of lithium chloride extends to D. reticulatus, confirmed to have 100% mortality at a relatively high minimum concentration of 1.38 M in vitro. The 24 h and 48 h median lethal concentration (LC50) values proved to be 0.654 M and 0.481 M, respectively, for this species. Our pilot study may contribute to a better understanding of the properties of lithium ion. Furthermore, it may elicit further studies aiming to reveal whether the different environmental mineral conditions may influence the D. reticulatus population. Further studies might reveal whether lithium has any possible veterinary relevance.}, keywords = {LITHIUM; Lithium Chloride; Acaricide; biocidal effect; dog tick}, year = {2023}, eissn = {2075-4450} } @article{MTMT:33282828, title = {Evaluation of acaricidal activity of lithium chloride against Tetranychus urticae (Acari: Tetranychidae)}, url = {https://m2.mtmt.hu/api/publication/33282828}, author = {Solti, Izabella and Kolics-Horváth, Éva and Keszthelyi, Sándor and Bacsi, Zsuzsanna and Staszny, Ádám and Nagy, Erzsébet and Taller, János and Mátyás, Kinga Klára and Kolics, Balázs}, doi = {10.3390/horticulturae8121127}, journal-iso = {HORTICULTURAE}, journal = {HORTICULTURAE}, volume = {8}, unique-id = {33282828}, keywords = {MORTALITY; LITHIUM; EFFICACY; horticulture; Two-spotted spider mite; alternative pesticide}, year = {2022}, eissn = {2311-7524}, orcid-numbers = {Keszthelyi, Sándor/0000-0001-7966-1001} } @article{MTMT:33208013, title = {Lithium Chloride Shows Effectiveness against the Poultry Red Mite (Dermanyssus gallinae)}, url = {https://m2.mtmt.hu/api/publication/33208013}, author = {Kolics, Balázs and Kolics-Horváth, Éva and Solti, Izabella and Bacsi, Zsuzsanna and Taller, János and Specziár, András and Mátyás, Kinga Klára}, doi = {10.3390/insects13111005}, journal-iso = {INSECTS}, journal = {INSECTS}, volume = {13}, unique-id = {33208013}, abstract = {The poultry red mite (Dermanyssus gallinae) is the main pest of poultry, causing severe problems by being a vector of several animal and human pathogens. The number of miticides is few, and their efficacy in practice implies problems of residues and resistance; therefore, the demand for a new and safe agent is constant. The present publication investigated the effectiveness of lithium chloride under in vitro conditions on poultry red mites. This chemical currently appears to be one of the most promising alternatives to study amongst potential applicants to treat varroosis, a fatal disease of honey bees. In Experiment I, the previously used experimental doses (5.52 M, 2.76 M, 1.38 M) on Varroa mites confirmed their in vitro activity on the poultry red mite. Three event times (uncontrolled movement, immobilisation and death) were recorded to base the response to treatment for each concentration. In Experiment II, the LD 50 value was calculated, i.e., the value at which 50% of the mites were killed by the treatment. This Experiment showed that the LD50 of lithium chloride = 0.265 M in the poultry red mite. It is to note that the study remained restricted to in vitro confirmation of lithium chloride’s effectiveness on the parasite. Thus, further extensive studies are needed to decide whether it has any relevance in practice against D. gallinae, and also to assess potential residue problems that could affect poultry products.}, keywords = {Lithium Chloride; Poultry red mite; Contact effect}, year = {2022}, eissn = {2075-4450}, orcid-numbers = {Bacsi, Zsuzsanna/0000-0003-0512-7820} } @article{MTMT:33067021, title = {Comparison of Alternative Application Methods for Anti-Varroa Lithium Chloride Treatments}, url = {https://m2.mtmt.hu/api/publication/33067021}, author = {Kolics, Balázs and Kolics-Horváth, Éva and Mátyás, Kinga Klára and Taller, János and Specziár, András}, doi = {10.3390/insects13070633}, journal-iso = {INSECTS}, journal = {INSECTS}, volume = {13}, unique-id = {33067021}, abstract = {Varroosis is one of the most dangerous threats to the bee industry but means of its treatment are still unsatisfactory. Lithium-based anti-Varroa treatments may provide an alternative, as this trace element can be a natural component of honey and is well tolerated by adult bees. However, it can be toxic to larvae and its use in beekeeping practice is not yet well understood. The present study aimed to investigate the efficacy of relevant application methods of acaricides used in beekeeping practice in brood-free conditions for lithium. Vaporisation proved to be an inefficient method of lithium treatment and killed only 9.9 ± 3.3% (mean ± SD) of mites in the hive. Lithium-impregnated paper strips showed moderate efficiency by killing 55.1 ± 26.2% of mites. The most effective way of applying lithium was the trickling method; different trickling treatments decreased the abundance of mites on average by 65 to 99.7%, depending on the applied dosage and the number of treatments. Repeated trickling treatments were more effective than single treatments, and they generally provided >90% efficiency. Experiments also proved that adding sugar to the trickling solution does not influence treatment efficiency. Thus, it is suggested that repeated and sugar-free trickling treatments with moderate lithium dosage could be the most rational methodology. Since lithium is not yet legalised in beekeeping practice, comprehensive studies are also needed to uncover the amount of lithium residue in bee products, depending on the treatment parameters.}, year = {2022}, eissn = {2075-4450} } @article{MTMT:32768677, title = {A DNA BASED METHOD TO DETERMINE THE ORIGIN OF HONEY PRODUCED BY APIS CERANA}, url = {https://m2.mtmt.hu/api/publication/32768677}, author = {Szepesi, Kinga and Heltai, Botond István and Kolics-Horváth, Éva and Mátyás, Kinga Klára and Kálmán, Nikoletta and Taller, János and Satoshi, Miyazaki and Kolics, Balázs}, journal-iso = {GEORGIKON AGRICUL}, journal = {GEORGIKON FOR AGRICULTURE: A MULTIDISCIPLINARY JOURNAL IN AGRICULTURAL SCIENCES}, volume = {26}, unique-id = {32768677}, issn = {0239-1260}, keywords = {Molecular marker; Adulteration; honey authenticity; Apis cerana; ethnic honey}, year = {2022}, pages = {20-34} } @article{MTMT:32082467, title = {Changes in Lithium Levels in Bees and Their Products Following Anti-Varroa Treatment}, url = {https://m2.mtmt.hu/api/publication/32082467}, author = {Kolics-Horváth, Éva and Sajtos, Zsófi and Mátyás, Kinga Klára and Szepesi, Kinga and Solti, Izabella and Németh, Gyöngyi and Taller, János and Baranyai, Edina and Specziár, András and Kolics, Balázs}, doi = {10.3390/insects12070579}, journal-iso = {INSECTS}, journal = {INSECTS}, volume = {12}, unique-id = {32082467}, abstract = {Simple Summary Varroosis caused by the ectoparasitic mite Varroa destructor has been the biggest threat to managed bee colonies over recent decades. Chemicals available to treat the disease imply problems of resistance, inconsistent efficacy, and residues in bee products. Recently, alongside novel compounds to defeat the pest, lithium chloride has been found to be effective. In this study, we found that lithium treatments leave beeswax residue-free. The possibility of decontamination in adult bees, bee bread, and uncapped honey was revealed. On the other hand, ripe honey was found to be affected by lithium administered via feeding. Case studies are necessary to uncover the level of exposition in harvested honey to estimate its potential risk once it becomes a registered veterinary medicine. The biggest threat to beekeeping is varroosis caused by the mite Varroa destructor. Chemicals available to treat this fatal disease may present problems of resistance or inconsistent efficacy. Recently, lithium chloride has appeared as a potential alternative. To date, the amount of residue lithium treatments may leave in honeybee products is poorly understood. Honeybees were fed with 25 mM lithiated sugar syrup, which was used in earlier studies. The accumulation and elimination of the lithium were monitored in bees and their products for 22 days. Lithium concentration increased in the entire body of the bees to day 4 post-treatment and then recovered rapidly to the control level. Lithium exposure was found to affect uncapped honey in the short term (<16 days), but ripe (capped) honey measured at the end of the trial remained affected. On the other hand, lithium treatment left beeswax lithium-free. Based on these data, we propose that comprehensive research on harvested honey is needed to decide on the veterinary use of lithium.}, keywords = {Lithium Chloride; Honey; Apis mellifera; Varroa destructor; beeswax}, year = {2021}, eissn = {2075-4450} } @article{MTMT:31911925, title = {Detection of Target-Site Herbicide Resistance in the Common Ragweed: Nucleotide Polymorphism Genotyping by Targeted Amplicon Sequencing}, url = {https://m2.mtmt.hu/api/publication/31911925}, author = {Kutasy, Barbara Júlia and Farkas, Zoltán and Kolics, Balázs and Decsi, Kincső and Hegedűs, Géza and Kovács, Judit and Taller, János and Tóth, Zoltán and Kálmán, Nikoletta and Kazinczi, Gabriella and Virág, Eszter Andrea}, doi = {10.3390/d13030118}, journal-iso = {DIVERSITY-BASEL}, journal = {DIVERSITY (BASEL)}, volume = {13}, unique-id = {31911925}, abstract = {Background: The spread of herbicide-resistance Ambrosia artemisiifolia threatens not only the production of agricultural crops, but also the composition of weed communities. The reduction of their spread would positively affect the biodiversity and beneficial weed communities in the arable habitats. Detection of resistant populations would help to reduce herbicide exposure which may contribute to the development of sustainable agroecosystems. Methods: This study focuses on the application of target-site resistance (TSR) diagnostic of A. artemisiifolia caused by different herbicides. We used targeted amplicon sequencing (TAS) on Illumina Miseq platform to detect amino acid changes in herbicide target enzymes of resistant and wild-type plants. Results: 16 mutation points of four enzymes targeted by four herbicide groups, such as Photosystem II (PSII), Acetohydroxyacid synthase (AHAS), 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) and protoporphyrinogen IX oxidase (PPO) inhibitors have been identified in common ragweed populations, so far. All the 16 mutation points were analyzed and identified. Out of these, two mutations were detected in resistant biotypes. Conclusions: The applied next-generation sequencing-targeted amplicon sequencing (NGS-TAS) method on A. artemisiifolia resistant and wild-type populations enable TSR detection of large sample numbers in a single reaction. The NGS-TAS provides information about the evolved herbicide resistance that supports the integrated weed control through the reduction of herbicide exposure which may preserve ecological properties in agroecosystems.}, keywords = {Ambrosia artemisiifolia; ALS; psbA; EPSPS; AHAS; targeted amplicon sequencing; target-site herbicide resistance}, year = {2021}, eissn = {1424-2818}, orcid-numbers = {Kutasy, Barbara Júlia/0000-0002-4790-5865; Kolics, Balázs/0000-0002-5687-3199; Tóth, Zoltán/0000-0003-4369-9774; Kálmán, Nikoletta/0000-0001-6827-9894; Kazinczi, Gabriella/0000-0002-8081-7824} } @article{MTMT:31831941, title = {Lithium chloride outperformed oxalic acid sublimation in a preliminary experiment for Varroa mite control in pre-wintering honey bee colonies}, url = {https://m2.mtmt.hu/api/publication/31831941}, author = {Kolics-Horváth, Éva and Specziár, András and Taller, János and Mátyás, Kinga Klára and Kolics, Balázs}, doi = {10.1556/004.2020.00060}, journal-iso = {ACTA VET HUNG}, journal = {ACTA VETERINARIA HUNGARICA}, volume = {68}, unique-id = {31831941}, issn = {0236-6290}, year = {2020}, eissn = {1588-2705}, pages = {370-373} }