TY - JOUR AU - Vályi, Péter AU - Wirth, Roland AU - Minárovits, János AU - Strang, Orsolya AU - Maróti, Gergely AU - Kovács, Kornél Lajos TI - The oral microbiome of a family including Papillon-Lefèvre-syndrome patients and clinically healthy members JF - BMC ORAL HEALTH J2 - BMC ORAL HEALTH VL - 24 PY - 2024 IS - 1 PG - 17 SN - 1472-6831 DO - 10.1186/s12903-024-03856-z UR - https://m2.mtmt.hu/api/publication/34558800 ID - 34558800 N1 - Department of Oral Diagnostics, Faculty of Dentistry, Semmelweis University, Szentkirályi u 47, Budapest, H1085, Hungary Department of Biotechnology, University of Szeged, Közép fasor 52, Szeged, H6726, Hungary Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Tisza L. krt 64, Szeged, H6720, Hungary Institute of Biophysics, Biological Research Center, Temesvári krt 62, Szeged, H6726, Hungary Institute of Plant Biology, Biological Research Center, Temesvári krt 62, Szeged, H6726, Hungary Export Date: 1 May 2024 Correspondence Address: Vályi, P.; Department of Oral Diagnostics, Szentkirályi u 47, Hungary; email: valyi.peter@dent.semmelweis-univ.hu Chemicals/CAS: dipeptidyl peptidase I, 9032-68-2 Funding details: European Regional Development Fund, ERDF, FK123899, GINOP-2.3.2-15-2016-00011, PD132145 Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFI Funding text 1: This work was supported by the European Regional Development Fund to a project led by JM; grant No.: GINOP-2.3.2-15-2016-00011. RW (PD132145) and GM (FK123899) received support from the National Research, Development and Innovation Office (NKFIH), Hungary. Funding text 2: Open access funding provided by Semmelweis University. This work was supported by the European Regional Development Fund to a project led by JM; grant No.: GINOP-2.3.2-15-2016-00011. RW (PD132145) and GM (FK123899) received support from the National Research, Development and Innovation Office (NKFIH), Hungary. AB - The oral microbiota composition of patients diagnosed with Papillon-Lefèvre-syndrome and treated for several years were compared to those existing in the oral cavity of the clinically healthy family members and a cohort of patients having various stages of chronic periodontitis. LA - English DB - MTMT ER - TY - JOUR AU - Szuhaj, Márk AU - Kakuk, Balázs AU - Wirth, Roland AU - Rákhely, Gábor AU - Kovács, Kornél Lajos AU - Bagi, Zoltán TI - Regulation of the methanogenesis pathways by hydrogen at transcriptomic level in time JF - APPLIED MICROBIOLOGY AND BIOTECHNOLOGY J2 - APPL MICROBIOL BIOT VL - 107 PY - 2023 IS - 20 SP - 6315 EP - 6324 PG - 10 SN - 0175-7598 DO - 10.1007/s00253-023-12700-3 UR - https://m2.mtmt.hu/api/publication/34130955 ID - 34130955 N1 - Funding Agency and Grant Number: Hungarian National Research, Development and Innovation Fund [20203.1.2-ZFR-KVG-202000009]; Hungarian NRDIF [PD 132145, K143198, FK123902, 20192.1.13-TET_IN-202000016]; University of Szeged Funding text: Open access funding provided by University of Szeged. This study has been supported in part by the Hungarian National Research, Development and Innovation Fund project 2020-3.1.2-ZFR-KVG-2020-00009. RW, ZB, and KLK received support from the Hungarian NRDIF fund projects, PD 132145, K143198, FK123902, and 2019-2.1.13-TET_IN-2020-00016. AB - The biomethane formation from 4 H-2 + CO2 by pure cultures of two methanogens, Methanocaldococcus fervens and Methanobacterium thermophilum, has been studied. The goal of the study was to understand the regulation of the enzymatic steps associated with biomethane biosynthesis by H-2, using metagenomic, pan-genomic, and transcriptomic approaches. Methanogenesis in the autotrophic methanogen M. fervens could be easily "switched off" and "switched on" by H-2/CO2 within about an hour. In contrast, the heterotrophic methanogen M. thermophilum was practically insensitive to the addition of the H-2/CO2 trigger although this methanogen also converted H-2/CO2 to CH4. From practical points of view, the regulatory function of H-2/CO2 suggests that in the power-to-gas (P2G) renewable excess electricity conversion and storage systems, the composition of the biomethane-generating methanogenic community is essential for sustainable operation. In addition to managing the specific hydrogenotrophic methanogenesis biochemistry, H-2/CO2 affected several, apparently unrelated, metabolic pathways. The redox-regulated overall biochemistry and symbiotic relationships in the methanogenic communities should be explored in order to make the P2G technology more efficient. LA - English DB - MTMT ER - TY - JOUR AU - Wirth, Roland AU - Bagi, Z AU - Shetty, Prateek AU - Szuhaj, M AU - Cheung, S AU - Kovács, Kornél Lajos AU - Maróti, Gergely TI - Machine-Learning-Guided Multi-Omics Investigation of Industrial-Scale Biogas Plants Reveals Inter-Kingdom Interactions and Stability of Methanogens JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 70 PY - 2023 IS - Supplement 1 SP - 87 EP - 88 PG - 2 SN - 1217-8950 UR - https://m2.mtmt.hu/api/publication/34092103 ID - 34092103 LA - English DB - MTMT ER - TY - JOUR AU - Márton, D AU - Farkas, M AU - Almalkawi, NM AU - Maróti, Gergely AU - Wirth, Roland AU - Táncsics, A AU - Szegi, Tamás András AU - Kriszt, B AU - Cserháti, M TI - Comparison of Soil Bacterial Communities Under Different Agricultural Land Uses JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 70 PY - 2023 IS - Supplement 1 SP - 33 EP - 33 PG - 1 SN - 1217-8950 UR - https://m2.mtmt.hu/api/publication/34092101 ID - 34092101 LA - English DB - MTMT ER - TY - JOUR AU - Wirth, Roland AU - Bagi, Zoltán AU - Shetty, Prateek AU - Szuhaj, Márk AU - Cheung, Teur Teur Sally AU - Kovács, Kornél Lajos AU - Maróti, Gergely TI - Inter-kingdom interactions and stability of methanogens revealed by machine-learning guided multi-omics analysis of industrial-scale biogas plants JF - ISME JOURNAL J2 - ISME J VL - 17 PY - 2023 IS - 8 SP - 1326 EP - 1339 PG - 14 SN - 1751-7362 DO - 10.1038/s41396-023-01448-3 UR - https://m2.mtmt.hu/api/publication/34011308 ID - 34011308 N1 - Institute of Plant Biology, Biological Research Centre, Szeged, Hungary Department of Biotechnology, University of Szeged, Szeged, Hungary Faculty of Water Sciences, University of Public Service, Baja, Hungary Cited By :2 Export Date: 15 November 2023 Correspondence Address: Maróti, G.; Institute of Plant Biology, Hungary; email: maroti.gergely@brc.hu Chemicals/CAS: Biofuels Funding details: FK123899, FK123902, FK142500, K143198, PD132145, ÚNKP-22-5-SZTE-537 Funding details: RRF-2.3.1-21-2022-00008 Funding details: Magyar Tudományos Akadémia, MTA, BO/00449/22, LP2020-5/2020 Funding details: Nemzeti Kutatási, Fejlesztési és Innovaciós Alap, NKFIA Funding text 1: This study has been supported in part by the Hungarian National Research, Development and Innovation Fund (NRDIF) projects: RW, BZ and GM received support from projects PD132145, FK142500, FK123902, FK123899, K143198 and ÚNKP-22-5-SZTE-537. This work was also supported by the Lendület-Programme (GM) and Bolyai Scholarship (WR) of the Hungarian Academy of Sciences (LP2020-5/2020 and BO/00449/22) and by the Széchenyi Plan Plus National Laboratory Programme (National Laboratory for Water Science and Water Security, RRF-2.3.1-21-2022-00008). BZ and KLK received support from 2020-3.1.2-ZFR-KVG-2020-00009 and 2019-2.1.13-TÉT_IN-2020-00016. Open access funding provided by ELKH Biological Research Center. AB - Multi-omics analysis is a powerful tool for the detection and study of inter-kingdom interactions, such as those between bacterial and archaeal members of complex biogas-producing microbial communities. In the present study, the microbiomes of three industrial-scale biogas digesters, each fed with different substrates, were analysed using a machine-learning guided genome-centric metagenomics framework complemented with metatranscriptome data. This data permitted us to elucidate the relationship between abundant core methanogenic communities and their syntrophic bacterial partners. In total, we detected 297 high-quality, non-redundant metagenome-assembled genomes (nrMAGs). Moreover, the assembled 16 S rRNA gene profiles of these nrMAGs showed that the phylum Firmicutes possessed the highest copy number, while the representatives of the archaeal domain had the lowest. Further investigation of the three anaerobic microbial communities showed characteristic alterations over time but remained specific to each industrial-scale biogas plant. The relative abundance of various microorganisms as revealed by metagenome data was independent from corresponding metatranscriptome activity data. Archaea showed considerably higher activity than was expected from their abundance. We detected 51 nrMAGs that were present in all three biogas plant microbiomes with different abundances. The core microbiome correlated with the main chemical fermentation parameters, and no individual parameter emerged as a predominant shaper of community composition. Various interspecies H 2 /electron transfer mechanisms were assigned to hydrogenotrophic methanogens in the biogas plants that ran on agricultural biomass and wastewater. Analysis of metatranscriptome data revealed that methanogenesis pathways were the most active of all main metabolic pathways. LA - English DB - MTMT ER - TY - JOUR AU - Farkas, Milán AU - Márton, Dalma AU - Maróti, Gergely AU - Wirth, Roland AU - Malatinszky, Ákos AU - Neveen, Majdi Almalkawi AU - Táncsics, András AU - Kriszt, Balázs AU - Cserháti, Mátyás TI - Screening for plant growth promoting properties in rizosphere microbial communities of dry Hungarian grasslands JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 70 PY - 2023 IS - Supplement: 1 SP - 15 EP - 15 PG - 1 SN - 1217-8950 UR - https://m2.mtmt.hu/api/publication/33656499 ID - 33656499 LA - English DB - MTMT ER - TY - JOUR AU - Lakatos, Gergely Ernő AU - Ranglová, Karolína AU - Bárcenas-Pérez, Daniela AU - Grivalský, Tomáš AU - Manoel, João Câmara AU - Mylenko, Mykola AU - Cheel, José AU - Nyári, József AU - Wirth, Roland AU - Kovács, Kornél Lajos AU - Kopecký, Jiří AU - Nedbalová, Linda AU - Masojídek, Jiří TI - Cold-adapted culturing of the microalga Monoraphidium sp. in thin-layer raceway pond for biomass production JF - ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS J2 - ALGAL RES VL - 69 PY - 2023 PG - 10 SN - 2211-9264 DO - 10.1016/j.algal.2022.102926 UR - https://m2.mtmt.hu/api/publication/33334260 ID - 33334260 N1 - Laboratory of Algal Biotechnology – Centre Algatech, Institute of Microbiology of the Czech Academy of Sciences, Opatovický mlýn, Třeboň, 379 81, Czech Republic Faculty of Science, University of South Bohemia, Branišovská 1760, České Budějovice, 370 05, Czech Republic Institute of Plant Biology, Biological Research Centre, Temesvári körút 62, Szeged, 6726, Hungary Department of Biotechnology, University of Szeged, Közép fasor 52, Szeged, 6726, Hungary Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Tisza Lajos körút 64-66, Szeged, H-6720, Hungary Department of Ecology, Faculty of Science, Charles University, Viničná 7, Prague, 128 44, Czech Republic Centre for Phycology, Institute of Botany of the Czech Academy of Sciences, Dukelská 135, Třeboň, 379 82, Czech Republic Cited By :3 Export Date: 5 January 2024 Correspondence Address: Lakatos, G.E.; Laboratory of Algal Biotechnology – Centre Algatech, Opatovický mlýn, Czech Republic; email: lakatos@alga.cz Funding details: TN010000048/03 Funding details: H2020 Marie Skłodowska-Curie Actions, MSCA, CZ.02.2.6910.0/0.0/19_074/0014484 Funding details: Technology Agency of the Czech Republic, TACR Funding details: Horizon 2020, 887227 Funding details: Institute of Botany of the Czech Academy of Sciences, RVO 67985939 Funding text 1: This research was supported by International mobility MSCA -IF III (project no. CZ.02.2.6910.0/0.0/19_074/0014484 awarded to G.E.L.) and the Institutional long-term research plan of the Institute of Botany of the Czech Academy of Sciences ( RVO 67985939 ). A part of this work was also funded by the Bio Based Industries Joint Undertaking (JU) – the European Union's Horizon 2020 research and innovation programme and the Bio Based Industries Consortium under grant agreement No 887227 (J.C., D.B-P., and J.K.) and the Technology Agency of the Czech Republic (NCK grant TN010000048/03 , J.C. and D.B-P.). Funding text 2: The authors thank Mr. Martin Lukeš for the GC-FID method development, Ms. Soňa Pekařová and Mr. Michal Bureš for technical assistance. This research was supported by International mobility MSCA-IF III (project no. CZ.02.2.6910.0/0.0/19_074/0014484 awarded to G.E.L.) and the Institutional long-term research plan of the Institute of Botany of the Czech Academy of Sciences (RVO 67985939). A part of this work was also funded by the Bio Based Industries Joint Undertaking (JU) – the European Union's Horizon 2020 research and innovation programme and the Bio Based Industries Consortium under grant agreement No 887227 (J.C. D.B-P. and J.K.) and the Technology Agency of the Czech Republic (NCK grant TN010000048/03, J.C. and D.B-P.). AB - Three cultivation regimes were tested in cold-adapted cultures of the green microalga Monoraphidium in an outdoor thin-layer raceway pond: cultivation under sunlight; its combination with continuous supplementary illumination; and nitrogen depletion using both light sources. The highest volumetric and areal productivity, 0.16 g L−1 d−1 and 3.22 g m−2 d−1, respectively corresponding to the specific growth rate μ of 0.191 d−1 were achieved when sunlight was combined with supplementary illumination. The maximum total fatty acid content, 20.29 % of DW, rich in oleic acid, 54 % of total fatty acid content, was achieved under nitrogen depletion stress. An outstanding amount of lutein, 26.39 mg lutein g−1 DW, was detected grown under sunlight in the first trial. From the harvested and fermented biomass in the second trial 236 mLN g−1oTS of methane was generated. LA - English DB - MTMT ER - TY - JOUR AU - Wirth, Roland AU - Bagi, Zoltán AU - Prateek, Shetty AU - Szuhaj, Márk AU - Sally, Cheung AU - Kovács, Kornél Lajos AU - Maróti, Gergely TI - Deep learning-guided genome resolved metagenome and metatranscriptome analysis of microbial community in three full-size biogas plants JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 70 PY - 2023 IS - Supplement: 1 SP - 50 PG - 1 SN - 1217-8950 UR - https://m2.mtmt.hu/api/publication/33284109 ID - 33284109 LA - English DB - MTMT ER - TY - CONF AU - Márton, Dalma AU - Farkas, Milán AU - Maróti, Gergely AU - Wirth, Roland AU - Malatinszky, Ákos AU - Almalkawi, Neveen AU - Táncsics, András AU - Kriszt, Balázs AU - Cserháti, Mátyás TI - Rhizosphere microbial communities of Poaceae species in dry Hungarian grasslands: screening for plant growth promoting properties T2 - Microbe-assisted crop production - opportunities, challenges and needs MICROPE 2022 PY - 2022 SP - 139 UR - https://m2.mtmt.hu/api/publication/33261888 ID - 33261888 LA - English DB - MTMT ER - TY - JOUR AU - Gősi, Péter AU - Rátkai, Sándor AU - Shetty, Prateek AU - Wirth, Roland AU - Maróti, Gergely AU - Oszvald, Ferenc AU - Knisz, Judit TI - Prediction of long-term localized corrosion rates in a carbon steel cooling water system is enhanced by metagenome analysis JF - ENGINEERING FAILURE ANALYSIS J2 - ENG FAIL ANAL VL - 141 PY - 2022 PG - 17 SN - 1350-6307 DO - 10.1016/j.engfailanal.2022.106733 UR - https://m2.mtmt.hu/api/publication/33069802 ID - 33069802 N1 - „A publikáció a Nemzeti Közszolgálati Egyetem 2020. évi Tématerületi Kiválóság Program keretében, a Fenntartható biztonság és társadalmi környezet elnevezésű projekt támogatásával valósult meg, az Innovációs és Technológiai Minisztérium Nemzeti Kutatási, Fejlesztési és Innovációs Alapból nyújtott támogatásával, a Nemzeti Kutatási, Fejlesztési és Innovációs Hivatal által kibocsátott támogatói okirat alapján.” Pályázat sorszáma: NKFIH-1273-6/2020 Támogató: NKFIH AB - To predict variation of maximum localized penetration with exposure time, long-term localized corrosion was assessed in an emergency cooling water system composed of two carbon steel pipelines of 700 mm diameter transporting raw river water at flow velocities of 1 m/s and 0.1 m/s. Field tests, visual inspection, ultrasonic testing, BART testing, SEM-EDS and metagenomic analyses were performed to assess the progress of long-term corrosion and determine the influence of microbes in the corrosion process. High corrosion was linked to sulphate reducing bacteria and potentially to methanogenic archaea in the low-velocity pipeline, while moderate corrosion was linked to non-sulphate reducing bacteria in the higher velocity pipeline. Using historical and literature data available as well as our own test results, an empirical model was developed to predict Maximum Localized Penetration change over time to be applied in the ageing management of cooling water systems. Molecular Microbiological Methods in combination with traditional techniques are useful tools in the ageing management of pipelines. By applying the empirical model developed and the approach presented, unexpected through-wall leaking can be avoided, thus, saving costs and assets. LA - English DB - MTMT ER -