TY - JOUR AU - Dudits, Dénes AU - Cseri, András AU - Török, Katalin AU - Vankova, Radomira AU - Dobrev, Petre I. AU - Sass, László AU - Steinbach, Gábor AU - Kelemen-Valkony, Ildikó AU - Zombori, Zoltán AU - Ferenc, Györgyi AU - Ayaydin, Ferhan TI - Manifestation of Triploid Heterosis in the Root System after Crossing Diploid and Autotetraploid Energy Willow Plants JF - GENES J2 - GENES-BASEL VL - 14 PY - 2023 IS - 10 PG - 17 SN - 2073-4425 DO - 10.3390/genes14101929 UR - https://m2.mtmt.hu/api/publication/34201709 ID - 34201709 N1 - Funding Agency and Grant Number: Hungarian government [GINOP-2.2.1-15-2017-00081, 2020-1.1.2-PIACI-KFI-2020-00117]; European Union [739593] Funding text: This work was funded by grant no. GINOP-2.2.1-15-2017-00081 and 2020-1.1.2-PIACI-KFI-2020-00117 from the Hungarian government. F.A. received support from the European Union's Horizon 2020 research and innovation program with grant number 739593. AB - Successful use of woody species in reducing climatic and environmental risks of energy shortage and spreading pollution requires deeper understanding of the physiological functions controlling biomass productivity and phytoremediation efficiency. Targets in the breeding of energy willow include the size and the functionality of the root system. For the combination of polyploidy and heterosis, we have generated triploid hybrids (THs) of energy willow by crossing autotetraploid willow plants with leading cultivars (Tordis and Inger). These novel Salix genotypes (TH3/12, TH17/17, TH21/2) have provided a unique experimental material for characterization of Mid-Parent Heterosis (MPH) in various root traits. Using a root phenotyping platform, we detected heterosis (TH3/12: MPH 43.99%; TH21/2: MPH 26.93%) in the size of the root system in soil. Triploid heterosis was also recorded in the fresh root weights, but it was less pronounced (MPH%: 9.63–19.31). In agreement with root growth characteristics in soil, the TH3/12 hybrids showed considerable heterosis (MPH: 70.08%) under in vitro conditions. Confocal microscopy-based imaging and quantitative analysis of root parenchyma cells at the division–elongation transition zone showed increased average cell diameter as a sign of cellular heterosis in plants from TH17/17 and TH21/2 triploid lines. Analysis of the hormonal background revealed that the auxin level was seven times higher than the total cytokinin contents in root tips of parental Tordis plants. In triploid hybrids, the auxin–cytokinin ratios were considerably reduced in TH3/12 and TH17/17 roots. In particular, the contents of cytokinin precursor, such as isopentenyl adenosine monophosphate, were elevated in all three triploid hybrids. Heterosis was also recorded in the amounts of active gibberellin precursor, GA19, in roots of TH3/12 plants. The presented experimental findings highlight the physiological basics of triploid heterosis in energy willow roots. LA - English DB - MTMT ER - TY - JOUR AU - Zombori, Zoltán AU - Török, Szabolcs AU - Nagy, Bettina AU - László, Nikolett AU - Sass, László AU - Jancsó, Mihály AU - Szabó, Gábor AU - Rádi, Feríz AU - Ferenc, Györgyi AU - Gyuricza, Csaba AU - Dudits, Dénes TI - Expression of triploid heterosis in the biomass productivity of energy willow plants under salinity stress JF - BIOMASS & BIOENERGY J2 - BIOMASS BIOENERGY VL - 174 PY - 2023 PG - 10 SN - 0961-9534 DO - 10.1016/j.biombioe.2023.106852 UR - https://m2.mtmt.hu/api/publication/33999425 ID - 33999425 N1 - Funding Agency and Grant Number: Hungarian government [2020-1.1.2-PIACI-KFI-2020-00117]; project 'Preparation for the transition to circular economy in the case of agricultural and green waste' of Environment and Energy Efficiency Operational Programme grant scheme of Ministry for Technology and Industry, Hungary [KEHOP-3.2.1-15-2021-00037] Funding text: This work was funded by grant 2020-1.1.2-PIACI-KFI-2020-00117 from the Hungarian government.The research was supported by the project 'Preparation for the transition to circular economy in the case of agricultural and green waste' of Environment and Energy Efficiency Operational Programme grant scheme of Ministry for Technology and Industry, Hungary under grant no.: KEHOP-3.2.1-15-2021-00037. LA - English DB - MTMT ER - TY - JOUR AU - Gombos, Magdolna AU - Hapek, Nóra AU - Kozma-Bognár, László AU - Grézal, Gábor AU - Zombori, Zoltán AU - Kiss, Edina AU - Györgyey, János TI - Limited water stress modulates expression of circadian clock genes in Brachypodium distachyon roots JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 13 PY - 2023 IS - 1 PG - 19 SN - 2045-2322 DO - 10.1038/s41598-022-27287-4 UR - https://m2.mtmt.hu/api/publication/33589414 ID - 33589414 AB - Organisms have evolved a circadian clock for the precise timing of their biological processes. Studies primarily on model dicots have shown the complexity of the inner timekeeper responsible for maintaining circadian oscillation in plants and have highlighted that circadian regulation is more than relevant to a wide range of biological processes, especially organ development and timing of flowering. Contribution of the circadian clock to overall plant fitness and yield has also long been known. Nevertheless, the organ- and species-specific functions of the circadian clock and its relation to stress adaptation have only recently been identified. Here we report transcriptional changes of core clock genes of the model monocot Brachypodium distachyon under three different light regimes (18:6 light:dark, 24:0 light and 0:24 dark) in response to mild drought stress in roots and green plant parts. Comparative monitoring of core clock gene expression in roots and green plant parts has shown that both phase and amplitude of expression in the roots of Brachypodium plants differ markedly from those in the green plant parts, even under well-watered conditions. Moreover, circadian clock genes responded to water depletion differently in root and shoot. These results suggest an organ-specific form and functions of the circadian clock in Brachypodium roots. LA - English DB - MTMT ER - TY - JOUR AU - Dudits, Dénes AU - Cseri, András AU - Török, Katalin AU - Sass, László AU - Zombori, Zoltán AU - Ferenc, Györgyi AU - Poór, Péter AU - Borbély, Péter Gábor AU - Czékus, Zalán AU - Vankova, Radomira AU - Dobrev, Petre AU - Szántó, Judit AU - Bagi, Zoltán AU - Kovács, Kornél Lajos TI - Triploid Hybrid Vigor in Above-Ground Growth and Methane Fermentation Efficiency of Energy Willow JF - FRONTIERS IN PLANT SCIENCE J2 - FRONT PLANT SCI VL - 13 PY - 2022 PG - 14 SN - 1664-462X DO - 10.3389/fpls.2022.770284 UR - https://m2.mtmt.hu/api/publication/32722153 ID - 32722153 N1 - Institute of Plant Biology, Biological Research Centre, Eötvös Loránd Research Network (ELKH), Szeged, Hungary Department of Plant Biology, University of Szeged, Szeged, Hungary Department of Biological Resources, Centre for Agricultural Research, Agricultural Institute, Martonvásár, Hungary Institute of Experimental Botany, Czech Academy of Sciences, Prague, Czech Republic UBM Feed Zrt. Pilisvörösvár, Hungary Department of Biotechnology, University of Szeged, Szeged, Hungary Export Date: 6 April 2022 Correspondence Address: Ferenc, G.; Institute of Plant Biology, Hungary; email: ferenc.gyorgyi@brc.hu Funding details: Magyarország Kormánya Funding text 1: This study was funded by grant nos. GINOP-2.2.1-15-2017-00081 and 2020-1.1.2-PIACI-KFI-2020-00117 from the Hungarian Government. The authors are very grateful to Feríz Rádi (Kiskun Kft. Kiskunhalas, Hungary) for providing the experimental field. LA - English DB - MTMT ER - TY - JOUR AU - Rádi, Feríz AU - Nagy, Bettina AU - Ferenc, Györgyi AU - Török, Katalin AU - Nagy, István AU - Zombori, Zoltán AU - Dudits, Dénes AU - Ayaydin, Ferhan TI - In planta test system for targeted cellular mutagenesis by injection of oligonucleotides to apical meristem of maize seedlings JF - ACTA PHYSIOLOGIAE PLANTARUM J2 - ACTA PHYSIOL PLANT VL - 43 PY - 2021 IS - 5 SN - 0137-5881 DO - 10.1007/s11738-021-03252-y UR - https://m2.mtmt.hu/api/publication/32006119 ID - 32006119 N1 - Institute of Plant Biology, Biological Research Centre, Eötvös Loránd Research Network, Szeged, Hungary Kiskun Research Center Ltd, Kiskunhalas, Hungary Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Institute of Biochemistry, Biological Research Centre, Eötvös Loránd Research Network, Szeged, Hungary ATGandCo Biotechnology Ltd, Mórahalom, Hungary Hungarian Centre of Excellence for Molecular Medicine (HCMM) Nonprofit Ltd, Szeged, Hungary Laboratory of Cellular Imaging, Biological Research Centre, Eötvös Loránd Research Network, Szeged, Hungary Cited By :1 Export Date: 20 April 2023 CODEN: APPLD Correspondence Address: Ferenc, G.; Institute of Plant Biology, Hungary; email: ferenc.gyorgyi@brc.hu LA - English DB - MTMT ER - TY - JOUR AU - Zombori, Zoltán AU - Nagy, Bettina AU - Mihály, Róbert AU - Pauk, János AU - Cseri, András AU - Sass, László AU - Horváth V., Gábor AU - Dudits, Dénes TI - RING-Type E3 Ubiqitin Ligase BarleyGenes(HvYrg1–2) Control Characteristics of Both Vegetative Organs and Seeds as Yield Components JF - PLANTS-BASEL J2 - PLANTS-BASEL VL - 9 PY - 2020 IS - 12 PG - 15 SN - 2223-7747 DO - 10.3390/plants9121693 UR - https://m2.mtmt.hu/api/publication/31680770 ID - 31680770 N1 - Institute of Plant Biology, Biological Research Centre, Temesvári krt. 62., Szeged, H-6726, Hungary Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, Szeged, H-6726, Hungary Laboratory of Cellular Imaging, Biological Research Center, Temesvári krt. 62., Szeged, H-6726, Hungary Cereal Research Non-Profit Ltd, Alsó Kikötő Sor 9., Szeged, H-6726, Hungary Institute of Genetics, Biological Research Centre, Temesvári krt. 62., Szeged, H-6726, Hungary Export Date: 11 February 2021 Correspondence Address: Dudits, D.; Institute of Plant Biology, Temesvári krt. 62., Hungary; email: dudits.denes@brc.hu Institute of Plant Biology, Biological Research Centre, Temesvári krt. 62., Szeged, H-6726, Hungary Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, Szeged, H-6726, Hungary Laboratory of Cellular Imaging, Biological Research Center, Temesvári krt. 62., Szeged, H-6726, Hungary Cereal Research Non-Profit Ltd, Alsó Kikötő Sor 9., Szeged, H-6726, Hungary Institute of Genetics, Biological Research Centre, Temesvári krt. 62., Szeged, H-6726, Hungary Export Date: 20 April 2021 Correspondence Address: Dudits, D.; Institute of Plant Biology, Temesvári krt. 62., Hungary; email: dudits.denes@brc.hu Institute of Plant Biology, Biological Research Centre, Temesvári krt. 62., Szeged, H-6726, Hungary Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, Szeged, H-6726, Hungary Laboratory of Cellular Imaging, Biological Research Center, Temesvári krt. 62., Szeged, H-6726, Hungary Cereal Research Non-Profit Ltd, Alsó Kikötő Sor 9., Szeged, H-6726, Hungary Institute of Genetics, Biological Research Centre, Temesvári krt. 62., Szeged, H-6726, Hungary Export Date: 22 April 2021 Correspondence Address: Dudits, D.; Institute of Plant Biology, Temesvári krt. 62., Hungary; email: dudits.denes@brc.hu Institute of Plant Biology, Biological Research Centre, Temesvári krt. 62., Szeged, H-6726, Hungary Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, Szeged, H-6726, Hungary Laboratory of Cellular Imaging, Biological Research Center, Temesvári krt. 62., Szeged, H-6726, Hungary Cereal Research Non-Profit Ltd, Alsó Kikötő Sor 9., Szeged, H-6726, Hungary Institute of Genetics, Biological Research Centre, Temesvári krt. 62., Szeged, H-6726, Hungary Export Date: 24 April 2021 Correspondence Address: Dudits, D.; Institute of Plant Biology, Temesvári krt. 62., Hungary; email: dudits.denes@brc.hu Institute of Plant Biology, Biological Research Centre, Temesvári krt. 62., Szeged, H-6726, Hungary Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, Szeged, H-6726, Hungary Laboratory of Cellular Imaging, Biological Research Center, Temesvári krt. 62., Szeged, H-6726, Hungary Cereal Research Non-Profit Ltd, Alsó Kikötő Sor 9., Szeged, H-6726, Hungary Institute of Genetics, Biological Research Centre, Temesvári krt. 62., Szeged, H-6726, Hungary Export Date: 30 August 2021 Correspondence Address: Dudits, D.; Institute of Plant Biology, Temesvári krt. 62., Hungary; email: dudits.denes@brc.hu AB - Previously, studies on RING-type E3 ubiquitin ligases in cereals were preferentially focused on GW2 genes primarily controlling seed parameters in rice and wheat. Here we report cloning two HvYrg genes from barley that share significant homology with rice GW2 gene. In antisense genotypes efficiency of gene silencing varied between genes and transgenic lines: ASHvYrg1: 30-50% and ASHvYrg2: 20-27%. Reduced activity of both genes altered shoot system with increasing number of side shoots. Changes in leaf width, weight, or plant weight and height reached significant levels in some transgenic lines. Lowering expression of the two barley HvYrg genes caused opposite responses in spike development. Plants with ASHvYrg1 gene construct showed earlier heading time and prolonged grain-filling period, while plants from ASHvYrg2 genotype flowered in delay. Digital imaging of root development revealed that down-regulation of HvYrg1 gene variant stimulated root growth, while ASHvYrg2 plants developed reduced root system. Comparison of seed parameters indicated an increase in thousand grain weight accompanied with longer and wider seed morphology. In summary we conclude that in contrast to inhibition of GW2 genes in rice and wheat plants, down-regulation of the barely HvYrg genes caused substantial changes in vegetative organs in addition to alteration of seed parameters. LA - English DB - MTMT ER - TY - JOUR AU - Gombos, Magdolna AU - Zombori, Zoltán AU - Szécsényi, Mária AU - Sándor, Györgyi AU - Kovács, Hajnalka Éva AU - Györgyey, János TI - Characterization of the LBD gene family in Brachypodium: a phylogenetic and transcriptional study. JF - PLANT CELL REPORTS J2 - PLANT CELL REP VL - 36 PY - 2017 IS - 1 SP - 61 EP - 79 PG - 19 SN - 0721-7714 DO - 10.1007/s00299-016-2057-0 UR - https://m2.mtmt.hu/api/publication/3144678 ID - 3144678 AB - KEY MESSAGE: An unambiguous nomenclature is proposed for the twenty-eight-member LOB domain transcription factor family in Brachypodium . Expression analysis provides unique transcript patterns that are characteristic of a wide range of organs and plant parts. LOB (lateral organ boundaries)-domain proteins define a family of plant-specific transcription factors involved in developmental processes from embryogenesis to seed production. They play a crucial role in shaping the plant architecture through coordinating cell fate at meristem to organ boundaries. Despite their high potential importance, our knowledge of them is limited, especially in the case of monocots. In this study, we characterized LOB domain protein coding genes (LBDs) of Brachypodium distachyon, a model plant for grasses, and present their phylogenetic relationships and an overall spatial expression study. In the Brachypodium genome database, 28 LBDs were found and then classified based on the presence of highly conserved LOB domain motif. Their transcript amounts were measured via quantitative real-time RT-PCR in 37 different plant parts from root tip to generative organs. Comprehensive phylogenetic analysis suggests that there are neither Brachypodium- nor monocot-specific lineages among LBDs, but there are differences in terms of complexity of subclasses between monocots and dicots. Although LBDs in Brachypodium have wide variation of tissue-specific expression and relative transcript levels, overall expression patterns show similarity to their counterparts in other species. The varying transcript profiles we observed support the hypothesis that Brachypodium LBDs have diverse but conserved functions in plant organogenesis. LA - English DB - MTMT ER - TY - JOUR AU - Zombori, Zoltán AU - Szécsényi, Mária AU - Györgyey, János TI - Different Approaches for Agrobacterium-mediated Genetic Transformation of Brachypodium distachyon, a New Model Plant for Temperate Grasses JF - ACTA BIOLOGICA SZEGEDIENSIS J2 - ACTA BIOL SZEGED VL - 55 PY - 2011 IS - 1 SP - 193 EP - 195 PG - 3 SN - 1588-385X UR - https://m2.mtmt.hu/api/publication/2220026 ID - 2220026 LA - English DB - MTMT ER - TY - JOUR AU - Cserháti, Mátyás AU - Túróczy, Zoltán AU - Zombori, Zoltán AU - Cserző, Miklós AU - Dudits, Dénes AU - Pongor, Sándor AU - Györgyey, János TI - Prediction of new abiotic stress genes in Arabidopsis thaliana and Oryza sativa according to enumeration-based statistical analysis JF - MOLECULAR GENETICS AND GENOMICS J2 - MOL GENET GENOMICS VL - 285 PY - 2011 IS - 5 SP - 375 EP - 391 PG - 17 SN - 1617-4615 DO - 10.1007/s00438-011-0605-4 UR - https://m2.mtmt.hu/api/publication/1921781 ID - 1921781 AB - Plants undergo an extensive change in gene regulation during abiotic stress. It is of great agricultural importance to know which genes are affected during stress response. The genome sequence of a number of plant species has been determined, among them Arabidopsis and Oryza sativa, whose genome has been annotated most completely as of yet, and are well-known organisms widely used as experimental systems. This paper applies a statistical algorithm for predicting new stress-induced motifs and genes by analyzing promoter sets co-regulated by abiotic stress in the previously mentioned two species. After identifying characteristic putative regulatory motif sequence pairs (dyads) in the promoters of 125 stress-regulated Arabidopsis genes and 87 O. sativa genes, these dyads were used to screen the entire Arabidopsis and O. sativa promoteromes to find related stress-induced genes whose promoters contained a large number of these dyads found by our algorithm. We were able to predict a number of putative dyads, characteristic of a large number of stress-regulated genes, some of them newly discovered by our algorithm and serve as putative transcription factor binding sites. Our new motif prediction algorithm comes complete with a stand-alone program. This algorithm may be used in motif discovery in the future in other species. The more than 1,200 Arabidopsis and 1,700 Orzya sativa genes found by our algorithm are good candidates for further experimental studies in abiotic stress. LA - English DB - MTMT ER - TY - JOUR AU - Bihari, Zoltán AU - Szvetnik, Attila AU - Szabó, Zsolt AU - Blastyák, András AU - Zombori, Zoltán AU - Balázs, Margit AU - Kiss, István TI - Functional analysis of long-chain n-alkane degradation by Dietzia spp. JF - FEMS MICROBIOLOGY LETTERS J2 - FEMS MICROBIOL LETT VL - 316 PY - 2011 IS - 2 SP - 100 EP - 107 PG - 8 SN - 0378-1097 DO - 10.1111/j.1574-6968.2010.02198.x UR - https://m2.mtmt.hu/api/publication/1921586 ID - 1921586 N1 - WoS:hiba:000287523500003 2019-03-03 18:32 első szerző nem egyezik AB - The genetic background of long-chain n-alkane degradation was investigated in detail in strain E1, a member of the genetically unexplored Dietzia genus. A suicide vector carrying a 518-bp alkB fragment was site-specifically integrated into the E1 chromosome, and the full alkB, as well as its chromosomal environment was sequenced after plasmid rescue experiments. Four out of the nine putative genes were strongly induced by long-chain n-alkanes in wild-type E1. ORF4 encoded a natural fusion protein consisting of an integral membrane alkane hydroxylase and a rubredoxin domain. The significance of the alkB-rub gene in n-alkane degradation was investigated in phenotypic tests, and the disruption mutant strain exhibited severely impaired growth on n-C-20 alkane carbon source. The mutation was successfully complemented with the expression of intact AlkB-Rub protein, the full-length form of which was detected by simultaneous immunoblotting. The presented data furnish the first experimental evidence of the in vivo existence of an AlkB-Rub natural fusion protein, which plays a major role in long-chain n-alkane degradation. LA - English DB - MTMT ER -