TY - JOUR AU - Szalontai, Klára Margit AU - Priskin, Katalin AU - Giricz, Zsófia AU - Mező, Béla AU - Pintér, Lajos AU - Pankotai, Tibor AU - Tiszlavicz, László AU - Sükösd, Farkas TI - Liquid biopsziával egy targetált kezeléssel befolyásolt tüdőtumor evolúciójának nyomában JF - KLINIKAI ONKOLÓGIA J2 - KLINIKAI ONKOLÓGIA VL - 9 PY - 2022 IS - Suppl.2 SP - 29 EP - 29 PG - 1 SN - 2064-5058 UR - https://m2.mtmt.hu/api/publication/33253080 ID - 33253080 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Sükösd, Farkas AU - Priskin, Katalin AU - Giricz, Zsófia AU - Mező, Béla AU - Pintér, Lajos AU - Pankotai, Tibor AU - Haracska, Lajos TI - Az újgenerációs szekvenáláson innen és túl: a tumorpanelek elemzéséből leszűrhető információk JF - KLINIKAI ONKOLÓGIA J2 - KLINIKAI ONKOLÓGIA VL - 9 PY - 2022 IS - Suppl.2 SP - 16 EP - 16 PG - 1 SN - 2064-5058 UR - https://m2.mtmt.hu/api/publication/33253071 ID - 33253071 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Takács, Bertalan Vilmos AU - Gyuris, Zoltán AU - Pintér, Lajos AU - Visnyovszki, Ádám AU - Enyedi, Márton Zsolt AU - Hajdú, Edit AU - Haracska, Lajos ED - Bánfalvi, Zsófia ED - Gócza, Elen ED - Olasz, Ferenc ED - Pál, Magda ED - Posta, Katalin ED - Várallyay, Éva TI - UNDERSTANDING THE EFFECTS OF COVID-19 ON THE MICROBIOME USING BIOINFORMATICS AND MACHINE LEARNING T2 - „FIBOK 2022” : Fiatal Biotechnológusok V. Országos Konferenciája PB - Magyar Agrár- és Élettudományi Egyetem, Genetika és Biotechnológia Intézet CY - Gödöllő SN - 9789632699998 PY - 2022 UR - https://m2.mtmt.hu/api/publication/33089595 ID - 33089595 LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Róbert AU - Balogh, Dávid AU - Pintér, Lajos AU - Jaksa, G. AU - Szeplaki, B. AU - Gráf, Alexandra AU - Győrfy, Zsuzsanna AU - Enyedi, Márton Zsolt AU - Kiss, Ernő AU - Haracska, Lajos AU - Unk, Ildikó TI - The Rad5 Helicase and RING Domains Contribute to Genome Stability through their Independent Catalytic Activities JF - JOURNAL OF MOLECULAR BIOLOGY J2 - J MOL BIOL VL - 434 PY - 2022 IS - 5 SN - 0022-2836 DO - 10.1016/j.jmb.2021.167437 UR - https://m2.mtmt.hu/api/publication/32606165 ID - 32606165 N1 - DNA Repair Research Group, Institute of Genetics, Biological Research Centre, Szeged, Eotvos Loránd Research Network, Szeged, H-6726, Hungary University of Szeged, Doctoral School of Biology, Hungary HCEMM-BRC Mutagenesis and Carcinogenesis Research Group, Institute of Genetics, Biological Research Centre, Szeged, Eotvos Loránd Research Network, Szeged, H-6726, Hungary Delta Bio 2000 Ltd., Szeged, H-6726, Hungary Export Date: 21 January 2022 CODEN: JMOBA Correspondence Address: Unk, I.; Biological Research Centre, Temesvari krt. 62., Hungary; email: unk.ildiko@brc.hu AB - Genomic stability is compromised by DNA damage that obstructs replication. Rad5 plays a prominent role in DNA damage bypass processes that evolved to ensure the continuation of stalled replication. Like its human orthologs, the HLTF and SHPRH tumor suppressors, yeast Rad5 has a RING domain that supports ubiquitin ligase activity promoting PCNA polyubiquitylation and a helicase domain that in the case of HLTF and Rad5 was shown to exhibit an ATPase-linked replication fork reversal activity. The RING domain is embedded in the helicase domain, confusing their separate investigation and the understanding of the exact role of Rad5 in DNA damage bypass. Particularly, it is still debated whether the helicase domain plays a catalytic or a non-enzymatic role during error-free damage bypass and whether it facilitates a function separately from the RING domain. In this study, through in vivo and in vitro characterization of domain-specific mutants, we delineate the contributions of the two domains to Rad5 function. Yeast genetic experiments and whole-genome sequencing complemented with biochemical assays demonstrate that the ubiquitin ligase and the ATPase-linked activities of Rad5 exhibit independent catalytic activities in facilitating separate pathways during error-free lesion bypass. Our results also provide important insights into the mutagenic role of Rad5 and indicate its tripartite contribution to DNA damage tolerance. © 2021 The Author(s) LA - English DB - MTMT ER - TY - JOUR AU - Priskin, Katalin AU - Pólya, Sára AU - Pintér, Lajos AU - Jaksa, Gábor AU - Csányi, Bernadett AU - Enyedi, Márton Zsolt AU - Sági-Zsigmond, Eszter AU - Sükösd, Farkas AU - Oláh, Orsolya AU - Kelemen, Gyöngyi AU - Nikolényi, Alíz AU - Uhercsák, Gabriella AU - Sántha, Dóra AU - Dobi, Ágnes AU - Szilágyi, Éva AU - Valicsek, Erzsébet AU - Torday, László AU - Tóth, Rozália AU - Kahán, Zsuzsanna AU - Haracska, Lajos TI - BC-Monitor: Towards a Routinely Accessible Circulating Tumor DNA-Based Tool for Real-Time Monitoring Breast Cancer Progression and Treatment Effectiveness JF - CANCERS J2 - CANCERS VL - 13 PY - 2021 IS - 14 PG - 19 SN - 2072-6694 DO - 10.3390/cancers13143489 UR - https://m2.mtmt.hu/api/publication/32101773 ID - 32101773 N1 - Funding Agency and Grant Number: European Union's Horizon 2020 research and innovation program [739593]; National Research, Development and Innovation OfficeNational Research, Development & Innovation Office (NRDIO) - Hungary [GINOP-2.3.2-15-2016-00001, GINOP-2.3.215-2016-00024, GINOP-2.3.2-15-2016-00026] Funding text: This project received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No. 739593. This work was also supported by the National Research, Development and Innovation Office (GINOP-2.3.2-15-2016-00001, GINOP-2.3.215-2016-00024, and GINOP-2.3.2-15-2016-00026). LA - English DB - MTMT ER - TY - JOUR AU - Fenteany, Gabriel AU - Gaur, Paras AU - Sharma, Gaurav AU - Pintér, Lajos AU - Kiss, Ernő AU - Haracska, Lajos TI - Robust high-throughput assays to assess discrete steps in ubiquitination and related cascades JF - BMC MOLECULAR AND CELL BIOLOGY J2 - BMC MOL CELL BIOL VL - 21 PY - 2020 IS - 1 PG - 16 SN - 2661-8850 DO - 10.1186/s12860-020-00262-5 UR - https://m2.mtmt.hu/api/publication/31268548 ID - 31268548 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office [GINOP-2.3.2-15-2016-00020, GINOP-2.3.2-15-201600026]; European Union's Horizon 2020 research and innovation programme [739593] Funding text: This work was supported by the National Research, Development and Innovation Office (GINOP-2.3.2-15-2016-00020 and GINOP-2.3.2-15-201600026). This project has also received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No. 739593. The funding bodies played no role in the design of the study, collection, analysis, interpretation of data, or writing of the manuscript. LA - English DB - MTMT ER - TY - JOUR AU - Kelemen, Gyöngyi AU - Tóth, R AU - Kószó, Renáta Lilla AU - Nikolényi, Alíz AU - Uhercsák, Gabriella AU - Sántha, Dóra AU - Valicsek, Erzsébet AU - Szilágyi, Éva AU - Torday, László AU - Pepó, J AU - Dobi, Ágnes AU - Priskin, Katalin AU - Pintér, Lajos AU - Pólya, Sára Erzsébet AU - Haracska, Lajos AU - Sükösd, Farkas AU - Kahán, Zsuzsanna TI - Keringő tumor DNS vizsgálat neoadjuváns kezelésben részesülő, illetve metasztatikus emlőrákos betegnél – kezdeti tapasztalataink JF - MAGYAR ONKOLÓGIA J2 - MAGYAR ONKOLÓGIA VL - 63 PY - 2019 IS - S1 SP - 33 EP - 33 PG - 1 SN - 0025-0244 UR - https://m2.mtmt.hu/api/publication/30938381 ID - 30938381 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Priskin, Katalin AU - Pintér, Lajos AU - Jaksa, Gábor AU - Pólya, Sára AU - Kahán, Zsuzsanna AU - Sükösd, Farkas AU - Haracska, Lajos TI - Folyékony biopszia a klinikai onkológiában – a precíziós orvoslás vonalvezetője JF - KLINIKAI ONKOLÓGIA J2 - KLINIKAI ONKOLÓGIA VL - 6 PY - 2019 IS - 1 SP - 65 EP - 72 PG - 8 SN - 2064-5058 UR - https://m2.mtmt.hu/api/publication/30846863 ID - 30846863 LA - Hungarian DB - MTMT ER - TY - CONF AU - Kelemen, Gyöngyi AU - Tóth, Rozália AU - Kószó, Renáta Lilla AU - Nikolényi, Alíz AU - Uhercsák, Gabriella AU - Sántha, Dóra AU - Valicsek, Erzsébet AU - Szilágyi, Éva AU - Torday, László AU - Pepó, Judit AU - Dobi, Ágnes AU - Priskin, Katalin AU - Pintér, Lajos AU - Pólya, Sára Erzsébet AU - Haracska, Lajos AU - Sükösd, Farkas AU - Kahán, Zsuzsanna TI - Keringő tumor DNS vizsgálat neoadjuváns kezelésben részesülő, illetve metasztatikus emlőrákos betegeknél – kezdeti tapasztalataink T2 - VIII. Szegedi Emlőrák Szimpózium PY - 2019 SP - 47 EP - 48 PG - 2 UR - https://m2.mtmt.hu/api/publication/30801735 ID - 30801735 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Mórocz, Mónika AU - Zsigmond, Eszter AU - Tóth, Róbert AU - Enyedi, Márton Zsolt AU - Pintér, Lajos AU - Haracska, Lajos TI - DNA-dependent protease activity of human Spartan facilitates replication of DNA-protein crosslink-containing DNA. JF - NUCLEIC ACIDS RESEARCH J2 - NUCLEIC ACIDS RES VL - 45 PY - 2017 IS - 6 SP - 3172 EP - 3188 PG - 17 SN - 0305-1048 DO - 10.1093/nar/gkw1315 UR - https://m2.mtmt.hu/api/publication/3166555 ID - 3166555 N1 - WoS:hiba:000398376200029 2019-11-12 23:14 cikkazonosító nem egyezik AB - Mutations in SPARTAN are associated with early onset hepatocellular carcinoma and progeroid features. A regulatory function of Spartan has been implicated in DNA damage tolerance pathways such as translesion synthesis, but the exact function of the protein remained unclear. Here, we reveal the role of human Spartan in facilitating replication of DNA-protein crosslink-containing DNA. We found that purified Spartan has a DNA-dependent protease activity degrading certain proteins bound to DNA. In concert, Spartan is required for direct DPC removal in vivo; we also show that the protease Spartan facilitates repair of formaldehyde-induced DNA-protein crosslinks in later phases of replication using the bromodeoxyuridin (BrdU) comet assay. Moreover, DNA fibre assay indicates that formaldehyde-induced replication stress dramatically decreases the speed of replication fork movement in Spartan-deficient cells, which accumulate in the G2/M cell cycle phase. Finally, epistasis analysis mapped these Spartan functions to the RAD6-RAD18 DNA damage tolerance pathway. Our results reveal that Spartan facilitates replication of DNA-protein crosslink-containing DNA enzymatically, as a protease, which may explain its role in preventing carcinogenesis and aging. LA - English DB - MTMT ER -