@article{MTMT:34720930,
	title = {A “torn bag mechanism” of small extracellular vesicle release via limiting membrane rupture of en bloc released amphisomes (amphiectosomes)},
	url = {https://m2.mtmt.hu/api/publication/34720930},
	author = {Visnovitz, Tamás and Lenzinger, Dorina and Koncz, Anna and Vizi, Péter Márk and Bárkai, Tünde and Visnovitzné Dr Vukman, Krisztina and Galinsoga, Alicia and Németh, Krisztina and Fletcher, Kelsey Aine and Komlósi, Zsolt and Cserép, Csaba and Dénes, Ádám and Lőrincz, Péter and Valcz, Gábor and Buzás, Edit Irén},
	doi = {10.7554/eLife.95828.3},
	journal-iso = {ELIFE},
	journal = {ELIFE},
	volume = {13},
	unique-id = {34720930},
	abstract = {Recent studies showed an unexpected complexity of extracellular vesicle (EV) biogenesis pathways. We previously found evidence that human colorectal cancer cells in vivo release large multivesicular body-like structures en bloc. Here, we tested whether this large EV type is unique to colorectal cancer cells. We found that all cell types we studied (including different cell lines and cells in their original tissue environment) released multivesicular large EVs (MV-lEVs). We also demonstrated that upon spontaneous rupture of the limiting membrane of the MV-lEVs, their intraluminal vesicles (ILVs) escaped to the extracellular environment by a ‘torn bag mechanism’. We proved that the MV-lEVs were released by ectocytosis of amphisomes (hence, we termed them amphiectosomes). Both ILVs of amphiectosomes and small EVs separated from conditioned media were either exclusively CD63 or LC3B positive. According to our model, upon fusion of multivesicular bodies with autophagosomes, fragments of the autophagosomal inner membrane curl up to form LC3B positive ILVs of amphisomes, while CD63 positive small EVs are of multivesicular body origin. Our data suggest a novel common release mechanism for small EVs, distinct from the exocytosis of multivesicular bodies or amphisomes, as well as the small ectosome release pathway.},
	year = {2025},
	eissn = {2050-084X},
	orcid-numbers = {Visnovitz, Tamás/0000-0002-7962-5083; Koncz, Anna/0000-0003-2511-2394; Németh, Krisztina/0000-0002-3825-2137; Fletcher, Kelsey Aine/0009-0001-1668-5222; Komlósi, Zsolt/0000-0002-4149-1497; Cserép, Csaba/0000-0001-5513-2471; Lőrincz, Péter/0000-0001-7374-667X; Valcz, Gábor/0000-0002-7109-3529; Buzás, Edit Irén/0000-0002-3744-206X}
}

@article{MTMT:35675779,
	title = {The protein cargo of extracellular vesicles correlates with the epigenetic aging clock of exercise sensitive DNAmFitAge},
	url = {https://m2.mtmt.hu/api/publication/35675779},
	author = {György, Bernadett and Szatmári, Réka and Ditrói, Tamás and Torma, Ferenc Gergely and Pálóczi, Krisztina and Balbisi, Mirjam and Visnovitz, Tamás and Koltai, Erika and Nagy, Péter and Buzás, Edit Irén and Horvath, Steve and Radák, Zsolt},
	doi = {10.1007/s10522-024-10177-9},
	journal-iso = {BIOGERONTOLOGY},
	journal = {BIOGERONTOLOGY},
	volume = {26},
	unique-id = {35675779},
	issn = {1389-5729},
	abstract = {Extracellular vesicles (EVs) are implicated in inter-organ communication, which becomes particularly relevant during aging and exercise. DNA methylation-based aging clocks reflect lifestyle and environmental factors, while regular exercise is known to induce adaptive responses, including epigenetic adaptations. Twenty individuals with High-fitness (aged 57.7 ± 9.8 years) and twenty Medium–Low-fitness (aged 57.5 ± 9.7 years) subjects provided blood samples. EVs were isolated from the samples using a size exclusion chromatography (SEC)-based method, and their protein content was analyzed by mass spectrometry (MS). Acceleration of the biological age estimator DNAmFitAge (AgeAccelFit) was associated with the protein cargo of EVs, whereas PhenoAge and GrimAge acceleration did not show a significant relationship. This finding suggests that the epigenetic aging-modulating role of exercise may involve inter-organ communication via EVs. Set Enrichment Analysis was performed to identify enriched Gene Ontology (GO) terms for sets of proteins that were either correlated with AgeAccelFit or detected exclusively in individuals with high levels of aerobic fitness. The protein cargo of EVs further suggests that inter-organ communication influences inflammation, the immune system, cellular repair, adhesion, metabolism and coagulation. Our findings help to understand the preventive role of exercise, which could be mediated in part by EVs.},
	year = {2025},
	eissn = {1573-6768},
	orcid-numbers = {György, Bernadett/0000-0002-3787-7338; Szatmári, Réka/0000-0002-1313-0766; Ditrói, Tamás/0009-0007-8148-2900; Pálóczi, Krisztina/0000-0001-7065-3582; Balbisi, Mirjam/0000-0002-6917-6974; Visnovitz, Tamás/0000-0002-7962-5083; Koltai, Erika/0000-0002-1370-2955; Nagy, Péter/0000-0003-3393-235X; Buzás, Edit Irén/0000-0002-3744-206X; Horvath, Steve/0000-0002-4110-3589; Radák, Zsolt/0000-0003-1297-6804}
}

@article{MTMT:35801901,
	title = {1-Pyrene Carboxylic Acid: An Internalization Enhancer for Short Oligoarginines},
	url = {https://m2.mtmt.hu/api/publication/35801901},
	author = {Bató, Csaba and Szabó, Ildikó and Yousef, M. and Lenzinger, Dorina and Grébecz, Fülöp Károly and Visnovitz, Tamás and Bősze, Szilvia and Bánóczi, Zoltán and Mező, Gábor},
	doi = {10.3390/ijms26052202},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {26},
	unique-id = {35801901},
	issn = {1661-6596},
	abstract = {Getting through the cell membrane is challenging, and transporting a therapeutic agent while entering the cell is even more complicated. Cell-penetrating peptides (CPPs) are valuable tools for solving this problem, although they have drawbacks. In this work, the synthesis and investigation of efficient CPPs are described. We used an aromatic group, 1-pyrene carboxylic acid (PCA), to enhance internalization. We designed oligoarginines to investigate the effect of PCA in different positions at the N-terminus or in the side chain. Our novel peptide derivatives showed remarkable internalization on tumor cell lines, and more than one endocytic pathway plays a role in their internalization mechanism. With this modification, there is an opportunity to design short oligoarginines that can rival well-known CPPs like octaarginine in internalization.},
	year = {2025},
	eissn = {1422-0067},
	orcid-numbers = {Szabó, Ildikó/0000-0002-9844-7841; Grébecz, Fülöp Károly/0009-0006-3357-6366; Visnovitz, Tamás/0000-0002-7962-5083; Bősze, Szilvia/0000-0001-9555-699X; Bánóczi, Zoltán/0000-0003-1880-4042; Mező, Gábor/0000-0002-7618-7954}
}

@CONFERENCE{MTMT:36152701,
	title = {Extracellular vesicles originated from mesenchymal cells of peritoneal dialysate reduce fibrosis},
	url = {https://m2.mtmt.hu/api/publication/36152701},
	author = {Szebeni, Beáta and Reusz, György and Szabó, Attila and Bokrossy, Péter and Veres-Székely, Apor and Pap, Domonkos and Szász, Csenge and Varga, Zoltán and Pállinger, Éva and Visnovitz, Tamás and Bernáth, Mária and Vannay, Ádám},
	booktitle = {ISEV 2025 Abstract book},
	unique-id = {36152701},
	year = {2025},
	pages = {373-374},
	orcid-numbers = {Szebeni, Beáta/0000-0001-7577-9803; Reusz, György/0000-0003-0396-043X; Szabó, Attila/0000-0001-7321-9861; Veres-Székely, Apor/0000-0002-6830-8779; Pap, Domonkos/0000-0002-1718-1210; Visnovitz, Tamás/0000-0002-7962-5083; Vannay, Ádám/0000-0001-7412-4733}
}

@article{MTMT:36188672,
	title = {Improvement in Transient Agarose Spot (TAS) Cell Migration Assay: Microplate-Based Detection and Evaluation},
	url = {https://m2.mtmt.hu/api/publication/36188672},
	author = {Veres-Székely, Apor and Szász, Csenge and Pap, Domonkos and Bokrossy, Péter and Lenzinger, Dorina and Visnovitz, Tamás and Mihály, Judith and Pálmai, Marcell and Varga, Zoltán and Őrfi, László and Szabó, Attila and Vannay, Ádám and Szebeni, Beáta},
	doi = {10.3390/ijms26125584},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {26},
	unique-id = {36188672},
	issn = {1661-6596},
	abstract = {Collective cell migration is crucial in various biological processes, including tumor progression and metastasis. The widely used scratch assay (wound healing assay) has limitations in throughput, reproducibility, and data analysis. To overcome these challenges, we previously developed the Transient Agarose Spot (TAS) assay, which enhanced assay precision and reproducibility. In this study, we present an improved microplate-based TAS assay. By using a microplate reader, we automated data acquisition, enabling the detection of cell migration in a 96-well plate format with greater throughput and accuracy. The new method applies Hoechst staining to label viable cells, providing a stable signal for kinetic analysis without compromising cell viability. We validated this approach with fluorophore-expressing cancer cells and demonstrated its ability to monitor dose-dependent effects of fetal bovine serum on cell migration. Additionally, we applied the microplate-based TAS assay to assess the anti-migratory effects of kinase inhibitors and mesenchymal stem cell-derived extracellular vesicles (EVs) on lung cancer cells. The assay accurately quantified migration inhibition and revealed the concentration-dependent effects of EVs, highlighting their potential as therapeutic agents. This microplate-based TAS assay provides a scalable, efficient, and cost-effective platform for high-throughput screening of cell migration and drug discovery, offering a robust alternative to traditional microscopy-based methods.},
	year = {2025},
	eissn = {1422-0067},
	orcid-numbers = {Veres-Székely, Apor/0000-0002-6830-8779; Pap, Domonkos/0000-0002-1718-1210; Visnovitz, Tamás/0000-0002-7962-5083; Pálmai, Marcell/0000-0001-9348-0733; Varga, Zoltán/0000-0002-5741-2669; Szabó, Attila/0000-0001-7321-9861; Vannay, Ádám/0000-0001-7412-4733; Szebeni, Beáta/0000-0001-7577-9803}
}

@CONFERENCE{MTMT:36202760,
	title = {TRANSLATION OF COMBINED DENSITY GRADIENT ULTRACENTRIFUGATION AND SIZE EXCLUSION CHROMATOGRAPHY-BASED EV ISOLATION FROM RAT TO HUMAN PLASMA FOR METABOLOMIC AND PROTEOMIC ANALYSES},
	url = {https://m2.mtmt.hu/api/publication/36202760},
	author = {Kapui, Dóra and Hambalkó, Szabolcs and Visnovitz, Tamás and Ferdinandy, Péter and Kovácsházi, Csenger and Giricz, Zoltán},
	booktitle = {International Pharmacology Conference: focus on drug discovery and innovation (HUPHAR 2025) : Oral & Poster Abstracts},
	unique-id = {36202760},
	year = {2025},
	pages = {205-205},
	orcid-numbers = {Visnovitz, Tamás/0000-0002-7962-5083; Ferdinandy, Péter/0000-0002-6424-6806; Kovácsházi, Csenger/0000-0003-0283-9486; Giricz, Zoltán/0000-0003-2036-8665}
}

@CONFERENCE{MTMT:36247255,
	title = {Improvement of Transient Agarose Spot (TAS) Cell Migration Assay: Microplate-based Detection and Evaluation},
	url = {https://m2.mtmt.hu/api/publication/36247255},
	author = {Veres-Székely, Apor and Szász, Csenge and Pap, Domonkos and Bokrossy, Péter and Lenzinger, Dorina and Visnovitz, Tamás and Mihály, Judith and Pálmai, Marcell and Varga, Zoltán and Őrfi, László and Szabó, Attila and Vannay, Ádám and Szebeni, Beáta},
	booktitle = {PhD Scientific Days 2025},
	unique-id = {36247255},
	year = {2025},
	orcid-numbers = {Veres-Székely, Apor/0000-0002-6830-8779; Pap, Domonkos/0000-0002-1718-1210; Visnovitz, Tamás/0000-0002-7962-5083; Szabó, Attila/0000-0001-7321-9861; Vannay, Ádám/0000-0001-7412-4733; Szebeni, Beáta/0000-0001-7577-9803}
}

@article{MTMT:36262347,
	title = {Improved Accessibility of Extracellular Vesicle Surface Molecules Upon Partial Removal of the Protein Corona by High Ionic Strength},
	url = {https://m2.mtmt.hu/api/publication/36262347},
	author = {Försönits, András and Tóth, Eszter Ágnes and Jezsoviczky, Sára and Bárkai, Tünde and Khamari, Delaram and Galinsoga, Alicia and Királyhidi, Panna and Kittel, Ágnes and Fazakas, J. and Lenzinger, Dorina and Hegyesi, Hargita and Osteikoetxea, Xabier and Visnovitz, Tamás and Pálóczi, Krisztina and Bősze, Szilvia and Buzás, Edit Irén},
	doi = {10.1002/jev2.70124},
	journal-iso = {J EXTRACELLULAR VESICL},
	journal = {JOURNAL OF EXTRACELLULAR VESICLES},
	volume = {14},
	unique-id = {36262347},
	abstract = {Recent studies have confirmed that a biomolecular corona forms around extracellular vesicles (EVs) in biofluids. However, there is limited data on how this adsorbed corona affects the accessibility of EV surface molecules. Here, we investigated various potential corona-stripping conditions for their ability to affect the immune detection of EVs. First, we artificially formed an EV corona around nascent HEK293T-PalmGFP cell-derived large EVs (lEVs) by incubating them with Cy5-labelled human plasma proteins. The co-localisation rate of plasma proteins and lEVs decreased significantly upon high-salt washing with NaCl, LiCl and KCl solutions, suggesting a considerable removal of the corona components. Additional evidence for corona modification was a significantly increased fluorescent annexin V binding to plasma lEVs and annexin V affinity capture of both THP1- and blood plasma-derived lEVs upon high-salt washing. A similar effect of high ionic strength was observed when THP1 lEVs were separated from a serum-containing medium, which allowed for corona formation, but not when EVs were produced under serum-free conditions. Using a MACSPlex kit and high-salt washing for small EVs from plasma and THP1 conditioned medium, we also demonstrated significantly improved immunodetection of 15 and 9 out of 37 surface markers, respectively. In this Technical Note, we present evidence that modifying the protein corona around EVs can significantly affect the immune detection of specific EV markers. © 2025 The Author(s). Journal of Extracellular Vesicles published by Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicles.},
	keywords = {Flow Cytometry; Blood plasma; Immunolabelling; extracellular vesicle; PROTEIN CORONA},
	year = {2025},
	eissn = {2001-3078},
	orcid-numbers = {Försönits, András/0000-0002-9298-8890; Jezsoviczky, Sára/0009-0009-7203-5302; Hegyesi, Hargita/0000-0002-8800-5169; Osteikoetxea, Xabier/0000-0003-3628-0174; Visnovitz, Tamás/0000-0002-7962-5083; Pálóczi, Krisztina/0000-0001-7065-3582; Bősze, Szilvia/0000-0001-9555-699X; Buzás, Edit Irén/0000-0002-3744-206X}
}

@article{MTMT:36282904,
	title = {Mimicking breast cancer tissue—3D bioprinted models in accurate drug sensitivity tests},
	url = {https://m2.mtmt.hu/api/publication/36282904},
	author = {Petővári, Gábor and Moldvai, Dorottya and Raffay, Regina and Dankó, Titanilla and Sztankovics, Dániel and Reszegi, Andrea and Miyaura, Risa and Gelencsér, Rebeka and Rókusz, András and Kriston, Csilla and Vilimi, Zsófia and Kállai-Szabó, Nikolett and Visnovitz, Tamás and Sebestyén, Anna},
	doi = {10.1002/VIW.20250092},
	journal-iso = {VIEW},
	journal = {VIEW},
	volume = {In press},
	unique-id = {36282904},
	issn = {2688-3988},
	abstract = {Three‐dimensional (3D) cell culture models derived from patient tumors are currently under development to recapitulate in vivo physiological conditions and assess therapeutic responses. In vitro models often fail to replicate the drug sensitivity observed in humans. In this study, a standardized in vitro culturing method using a 3D bioprinted breast cancer tumor model was established. We compared traditional two‐dimensional (2D), spheroid, and 3D bioprinted in vitro models as well as in vivo growing syngeneic or xenograft tumors. Our aim was to determine whether 3D bioprinted in vitro cultures can represent tissue heterogeneity, growth capacity, and/or drug response as potential tools for personalized drug sensitivity tests. Our findings demonstrated that 3D bioprinted models closely mimic in vivo tumor morphology and drug responses, outperforming 2D cultures and patient‐derived xenografts (PDX) in severe combined immunodeficiency mice. Additionally, 3D bioprinted models showed similar drug sensitivity to syngeneic tumors regrown in BALB/c mice, highlighting their potential for better therapeutic response predictions. Our results support the use of 3D bioprinted tumor models for personalized oncology. The presented approach could significantly advance personalized cancer therapy by using 3D bioprinted tumor tissues, offering a more accurate representation of tumor behavior and treatment efficacy compared to currently used PDX models.},
	year = {2025},
	eissn = {2688-268X},
	pages = {In press-16},
	orcid-numbers = {Petővári, Gábor/0000-0002-1957-2864; Dankó, Titanilla/0000-0002-7419-4560; Reszegi, Andrea/0000-0001-6902-7883; Vilimi, Zsófia/0000-0002-9241-5267; Kállai-Szabó, Nikolett/0000-0002-8164-3993; Visnovitz, Tamás/0000-0002-7962-5083; Sebestyén, Anna/0000-0001-8814-4794}
}

@article{MTMT:34850199,
	title = {Effect of the 35 nm and 70 nm Size Exclusion Chromatography (SEC) Column and Plasma Storage Time on Separated Extracellular Vesicles},
	url = {https://m2.mtmt.hu/api/publication/34850199},
	author = {György, Bernadett and Pálóczi, Krisztina and Balbisi, Mirjam and Turiák, Lilla and Drahos, László and Visnovitz, Tamás and Koltai, Erika and Radák, Zsolt},
	doi = {10.3390/cimb46050264},
	journal-iso = {CURR ISSUES MOL BIOL},
	journal = {CURRENT ISSUES IN MOLECULAR BIOLOGY},
	volume = {46},
	unique-id = {34850199},
	issn = {1467-3037},
	abstract = {The technical difficulty of separating extracellular vesicles (EVs) from plasma proteins in human blood presents a significant hurdle in EV research, particularly during nano ultra-high-performance liquid chromatography–tandem mass spectrometric (UHPLC-MS/MS) analysis, where detecting “vesicular” proteins among abundant plasma proteins is challenging. Standardisation is a pressing issue in EV research, prompting collaborative global efforts to address it. While the MISEV guidelines offer valuable recommendations, unanswered questions remain, particularly regarding sample storage. We compared size exclusion chromatography (SEC) columns with pore sizes of 35 nm and 70 nm to identify fractions with minimal contaminating proteins and the highest concentration of small EVs (sEVs). Following column selection, we explored potential differences in the quality and quantity of sEVs isolated from platelet-free plasma (PFP) after long-term storage at −80 °C (>2.5 years) compared to freshly drawn blood. Our methodologically rigorous study indicates that prolonged storage, under correct storage and processing conditions, does not compromise sEV quality. Both columns effectively isolated vesicles, with the 70 nm column exhibiting a higher abundance of “vesicular” proteins. We propose a relatively rapid and moderately efficient protocol for obtaining a comparatively pure sEV fraction from plasma, facilitating sEV processing in clinical trials.},
	year = {2024},
	eissn = {1467-3045},
	pages = {4337-4357},
	orcid-numbers = {György, Bernadett/0000-0002-3787-7338; Pálóczi, Krisztina/0000-0001-7065-3582; Balbisi, Mirjam/0000-0002-6917-6974; Turiák, Lilla/0000-0002-2139-8156; Drahos, László/0000-0001-9589-6652; Visnovitz, Tamás/0000-0002-7962-5083; Koltai, Erika/0000-0002-1370-2955; Radák, Zsolt/0000-0003-1297-6804}
}