@article{MTMT:35526600,
	title = {Formulation and characterization of nanofibrous scaffolds incorporating extracellular vesicles loaded with curcumin},
	url = {https://m2.mtmt.hu/api/publication/35526600},
	author = {Nochta-Kazsoki, Adrienn Katalin and Németh, Krisztina and Visnovitz, Tamás and Lenzinger, Dorina and Buzás, Edit Irén and Zelkó, Romána},
	doi = {10.1038/s41598-024-79277-3},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {14},
	unique-id = {35526600},
	year = {2024},
	eissn = {2045-2322},
	orcid-numbers = {Nochta-Kazsoki, Adrienn Katalin/0000-0002-0611-3124; Németh, Krisztina/0000-0002-3825-2137; Visnovitz, Tamás/0000-0002-7962-5083; Buzás, Edit Irén/0000-0002-3744-206X; Zelkó, Romána/0000-0002-5419-9137}
}

@CONFERENCE{MTMT:35172786,
	title = {Nitric oxide accumulates under iron deficiency in chloroplasts},
	url = {https://m2.mtmt.hu/api/publication/35172786},
	author = {Zelenyánszki, Helga and Klencsár, Zoltán and Visnovitz, Tamás and Kolbert, Zsuzsanna and Solti, Ádám},
	booktitle = {9th Plant Nitric Oxide International Meeting (PNO9)},
	unique-id = {35172786},
	abstract = {Iron (Fe) is an essential cofactor of multiple enzymes, but to avoid its hazardous effects on oxidative stress, signalling of the Fe status, especially in plant cells organelles is essential, although hardly known. Since 80–90% of Fe in the mesophyll cells is targeted to the chloroplasts to operate the photosynthetic apparatus, the control over plastidial Fe status has prime importance. Sensing the cytoplasmic Fe is based on hemerythrin motif-containing E3 ubiquitin ligases that are negative regulators of Fe deficiency responses. Indeed, no mechanisms are described that monitor the Fe status of the organelles. In vitro chloroplast Fe uptake (Solti et al., 2012; Müller et al. 2019) and ferric chelate reductase enzyme studies (Solti et al. 2014; Sági-Kazár et al. 2021) suggests that Fe in the chloroplasts has a feedback regulatory effect on the affinity of the plastidial Fe acquisition machinery. Nitric oxide (NO) is long known to regulate Fe uptake of roots, but also generated in chloroplasts under increasing Fe availability. It is involved in post translation modification of proteins that directly affect the function of those. Since the synthesis and accumulation of NO in chloroplasts has not been confirmed so far, we aimed here to detect and quantify NO in response to altered Fe nutrition chloroplasts.
		Sugar beet (Beta vulgaris cv. Orbis) model was grown on Fe(III)-EDTA source. Fe deficiency (Fe-def) was achieved with a complete depletion of Fe in the four-leaf stage. Mesophyll cell protoplasts and chloroplasts were isolated. Abundance of the NO signal in the protoplast population and the subcellular NO locations in the protoplasts were analysed applying DAF-FM diacetate in fluorescence activated cell sorting and confocal microscopy, respectively. The proportion of DAF-FM positive protoplasts significantly increased under iron deficiency, together with a higher apparent DAF-FM signal intensity in the protoplasts. Applying BioXol membrane staining of the chloroplast envelopes, chloroplast stroma origin of the DAF-FM signal was approved. DAF-FM signal of isolated chloroplasts was analysed by epifluorescent microscope, also indicating an increased ratio and pixel density of DAF-FM positive chloroplasts under Fe-def compared to the control. Electron paramagnetic resonance (EPR) spectroscopy based semiquantitative NO determination, applying N-methyl-D-glucamine dithiocarbamate spin trap and 150 K environment indicated a concentration of NO in the Fe-def chloroplasts around the lower detection limit, that of attomol NO chloroplast-1 dimension. NO in the control chloroplasts remained below the detection limit. Tyr nitration pattern detected by anti-nitro-Tyr immunoblotting of total chloroplast protein showed no significant changes in the Tyr nitration status indicating that nitrosative stress level has not increased significantly.
		
		This work was supported by the grant financed by the National Research, Development and Innovation Office, Hungary (NKFIH K-135607). Á.S. was also supported by the Bolyai János Research Scholarship of the Hungarian Academy of Sciences (BO/00113/23/8).
		
		References
		Müller et al. (2019) Planta 249:751.
		Sági-Kazár et al. (2021) Front Plant Sci 12:748.
		Solti et al. (2014) New Phytol 202:920.
		Solti et al. (2012) Plant Physiol Biochem 52:91},
	year = {2024},
	pages = {7-7},
	orcid-numbers = {Zelenyánszki, Helga/0000-0001-6768-3748; Klencsár, Zoltán/0000-0003-0175-7024; Visnovitz, Tamás/0000-0002-7962-5083; Kolbert, Zsuzsanna/0000-0002-7819-4672; Solti, Ádám/0000-0003-1479-5492}
}

@article{MTMT:35164265,
	title = {Detection of exogenous siRNA inside sweet corn bundle sheath cells and the RNAi dynamics in the early stage of Maize dwarf mosaic virus infection.},
	url = {https://m2.mtmt.hu/api/publication/35164265},
	author = {Balassa, Kinga and Balassa, György and Almási, Asztéria and Visnovitz, Tamás and Rudnóy, Szabolcs},
	doi = {10.1007/s12298-024-01500-2},
	journal-iso = {PHYSIOL MOL BIOL PLANTS},
	journal = {PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS},
	volume = {30},
	unique-id = {35164265},
	issn = {0971-5894},
	year = {2024},
	eissn = {0974-0430},
	pages = {1265-1276},
	orcid-numbers = {Balassa, Kinga/0000-0003-1537-362X; Visnovitz, Tamás/0000-0002-7962-5083; Rudnóy, Szabolcs/0000-0002-2726-1687}
}

@article{MTMT:34850199,
	title = {Effect of the 35 nm and 70 nm Size Exclusion Chromatography (SEC) Column and Plasma Storage Time on Separated Extracellular Vesicles},
	url = {https://m2.mtmt.hu/api/publication/34850199},
	author = {György, Bernadett and Pálóczi, Krisztina and Balbisi, Mirjam and Turiák, Lilla and Drahos, László and Visnovitz, Tamás and Koltai, Erika and Radák, Zsolt},
	doi = {10.3390/cimb46050264},
	journal-iso = {CURR ISSUES MOL BIOL},
	journal = {CURRENT ISSUES IN MOLECULAR BIOLOGY},
	volume = {46},
	unique-id = {34850199},
	issn = {1467-3037},
	abstract = {The technical difficulty of separating extracellular vesicles (EVs) from plasma proteins in human blood presents a significant hurdle in EV research, particularly during nano ultra-high-performance liquid chromatography–tandem mass spectrometric (UHPLC-MS/MS) analysis, where detecting “vesicular” proteins among abundant plasma proteins is challenging. Standardisation is a pressing issue in EV research, prompting collaborative global efforts to address it. While the MISEV guidelines offer valuable recommendations, unanswered questions remain, particularly regarding sample storage. We compared size exclusion chromatography (SEC) columns with pore sizes of 35 nm and 70 nm to identify fractions with minimal contaminating proteins and the highest concentration of small EVs (sEVs). Following column selection, we explored potential differences in the quality and quantity of sEVs isolated from platelet-free plasma (PFP) after long-term storage at −80 °C (>2.5 years) compared to freshly drawn blood. Our methodologically rigorous study indicates that prolonged storage, under correct storage and processing conditions, does not compromise sEV quality. Both columns effectively isolated vesicles, with the 70 nm column exhibiting a higher abundance of “vesicular” proteins. We propose a relatively rapid and moderately efficient protocol for obtaining a comparatively pure sEV fraction from plasma, facilitating sEV processing in clinical trials.},
	year = {2024},
	eissn = {1467-3045},
	pages = {4337-4357},
	orcid-numbers = {György, Bernadett/0000-0002-3787-7338; Pálóczi, Krisztina/0000-0001-7065-3582; Balbisi, Mirjam/0000-0002-6917-6974; Turiák, Lilla/0000-0002-2139-8156; Drahos, László/0000-0001-9589-6652; Visnovitz, Tamás/0000-0002-7962-5083; Koltai, Erika/0000-0002-1370-2955; Radák, Zsolt/0000-0003-1297-6804}
}

@article{MTMT:34720930,
	title = {A “torn bag mechanism” of small extracellular vesicle release via limiting membrane rupture of en bloc released amphisomes (amphiectosomes)},
	url = {https://m2.mtmt.hu/api/publication/34720930},
	author = {Visnovitz, Tamás and Lenzinger, Dorina and Koncz, Anna and Vizi, Péter M and Bárkai, Tünde and Visnovitzné Dr Vukman, Krisztina and Galinsoga, Alicia and Németh, Krisztina and Fletcher, Kelsey and Komlósi, Zsolt and Lőrincz, Péter and Valcz, Gábor and Buzás, Edit Irén},
	doi = {10.7554/eLife.95828.1},
	journal-iso = {ELIFE},
	journal = {ELIFE},
	volume = {13},
	unique-id = {34720930},
	issn = {2050-084X},
	year = {2024},
	eissn = {2050-084X},
	orcid-numbers = {Visnovitz, Tamás/0000-0002-7962-5083; Koncz, Anna/0000-0003-2511-2394; Németh, Krisztina/0000-0002-3825-2137; Komlósi, Zsolt/0000-0002-4149-1497; Lőrincz, Péter/0000-0001-7374-667X; Valcz, Gábor/0000-0002-7109-3529; Buzás, Edit Irén/0000-0002-3744-206X}
}

@misc{MTMT:34692287,
	title = {A “torn bag mechanism” of small extracellular vesicle release via limiting membrane rupture of en bloc released amphisomes (amphiectosomes)},
	url = {https://m2.mtmt.hu/api/publication/34692287},
	author = {Visnovitz, Tamás and Lenzinger, Dorina and Koncz, Anna and Vizi, Péter M and Bárkai, Tünde and Vukman, Krisztina V and Galinsoga, Alicia and Németh, Krisztina and Fletcher, Kelsey and Komlósi, Zsolt I},
	unique-id = {34692287},
	year = {2024},
	pages = {2024},
	orcid-numbers = {Visnovitz, Tamás/0000-0002-7962-5083; Koncz, Anna/0000-0003-2511-2394}
}

@article{MTMT:34567532,
	title = {Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches},
	url = {https://m2.mtmt.hu/api/publication/34567532},
	author = {Welsh, Joshua A. and Goberdhan, Deborah C. I. and O'Driscoll, Lorraine and Buzás, Edit Irén and Blenkiron, Cherie and Bussolati, Benedetta and Cai, Houjian and Di Vizio, Dolores and Driedonks, Tom A. P. and Erdbrügger, Uta and Falcon‐Perez, Juan M. and Fu, Qing‐Ling and Hill, Andrew F. and Lenassi, Metka and Lim, Sai Kiang and Mahoney, Mỹ G. and Mohanty, Sujata and Möller, Andreas and Nieuwland, Rienk and Ochiya, Takahiro and Sahoo, Susmita and Torrecilhas, Ana C. and Zheng, Lei and Zijlstra, Andries and Abuelreich, Sarah and Bagabas, Reem and Bergese, Paolo and Bridges, Esther M. and Brucale, Marco and Burger, Dylan and Carney, Randy P. and Cocucci, Emanuele and Colombo, Federico and Crescitelli, Rossella and Hanser, Edveena and Harris, Adrian L. and Haughey, Norman J. and Hendrix, An and Ivanov, Alexander R. and Jovanovic‐Talisman, Tijana and Kruh‐Garcia, Nicole A. and Ku'ulei‐Lyn Faustino, Vroniqa and Kyburz, Diego and Lässer, Cecilia and Lennon, Kathleen M. and Lötvall, Jan and Maddox, Adam L. and Martens‐Uzunova, Elena S. and Mizenko, Rachel R. and Newman, Lauren A. and Ridolfi, Andrea and Rohde, Eva and Rojalin, Tatu and Rowland, Andrew and Saftics, Andras and Sandau, Ursula S. and Saugstad, Julie A. and Shekari, Faezeh and Swift, Simon and Ter‐Ovanesyan, Dmitry and Tosar, Juan P. and Useckaite, Zivile and Valle, Francesco and Varga, Zoltán and van der Pol, Edwin and van Herwijnen, Martijn J. C. and Wauben, Marca H. M. and Wehman, Ann M. and Williams, Sarah and Zendrini, Andrea and Zimmerman, Alan J. and Théry, Clotilde and Witwer, Kenneth W. and Beke-Somfai, Tamás and Szigyártó, Imola Csilla and Haseeb, Zubair},
	doi = {10.1002/jev2.12404},
	journal-iso = {J EXTRACELLULAR VESICL},
	journal = {JOURNAL OF EXTRACELLULAR VESICLES},
	volume = {13},
	unique-id = {34567532},
	abstract = {Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year‐on‐year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non‐vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.},
	year = {2024},
	eissn = {2001-3078},
	orcid-numbers = {Welsh, Joshua A./0000-0002-1097-9756; Goberdhan, Deborah C. I./0000-0003-0645-6714; Buzás, Edit Irén/0000-0002-3744-206X; Bussolati, Benedetta/0000-0002-3663-5134; Cai, Houjian/0000-0003-4887-2652; Falcon‐Perez, Juan M./0000-0003-3133-0670; Hill, Andrew F./0000-0001-5581-2354; Lenassi, Metka/0000-0002-9488-6855; Mohanty, Sujata/0000-0002-0047-4914; Nieuwland, Rienk/0000-0002-5671-3400; Ochiya, Takahiro/0000-0002-0776-9918; Sahoo, Susmita/0000-0002-7279-1564; Torrecilhas, Ana C./0000-0001-5724-2199; Zheng, Lei/0000-0003-2576-8780; Zijlstra, Andries/0000-0001-8460-8803; Brucale, Marco/0000-0001-7244-4389; Carney, Randy P./0000-0001-8193-1664; Crescitelli, Rossella/0000-0002-1714-3169; Haughey, Norman J./0000-0001-5194-4122; Martens‐Uzunova, Elena S./0000-0002-5363-2525; Newman, Lauren A./0000-0003-3303-1666; Rohde, Eva/0000-0001-8692-886X; Sandau, Ursula S./0000-0002-3646-7089; Saugstad, Julie A./0000-0002-2996-9611; Shekari, Faezeh/0000-0001-6026-5412; Tosar, Juan P./0000-0002-2021-2479; Varga, Zoltán/0000-0002-5741-2669; Wauben, Marca H. M./0000-0003-0360-0311; Wehman, Ann M./0000-0001-9826-4132; Zimmerman, Alan J./0000-0001-6280-4790; Théry, Clotilde/0000-0001-8294-6884; Witwer, Kenneth W./0000-0003-1664-4233; Bodnár, Bernadett Réka/0000-0003-3347-9225; Bukva, Mátyás/0000-0002-5225-0285; Buzás, Edit Irén/0000-0002-3744-206X; Buzás, Krisztina/0000-0001-8933-2033; Dobra, Gabriella/0000-0002-2814-7720; Försönits, András/0000-0002-9298-8890; Ghosal, Sayam/0000-0001-6618-930X; Gyukity-Sebestyén, Edina/0000-0003-1383-6301; Koncz, Anna/0000-0003-2511-2394; Lőrincz, Márton Ákos/0000-0002-2819-5116; Németh, Krisztina/0000-0002-3825-2137; Oláh, Attila/0000-0003-4122-5639; Osteikoetxea, Xabier/0000-0003-3628-0174; Pálóczi, Krisztina/0000-0001-7065-3582; Stepanova, Ganna/0000-0002-8285-2762; Visnovitz, Tamás/0000-0002-7962-5083; Wiener, Zoltán/0000-0001-7056-4926; Harmati, Mária/0000-0002-4875-5723; Hegyesi, Hargita/0000-0002-8800-5169}
}

@misc{MTMT:34486423,
	title = {Nanoinjection of fluorescent and gold nanoparticles to single live cells by robotic fluidic force microscopy},
	url = {https://m2.mtmt.hu/api/publication/34486423},
	author = {Kovács, Kinga Dóra and Visnovitz, Tamás and Kanyó, Nicolett and Gerecsei, Tamás and Péter, Beatrix and Lagzi, István and Kurunczi, Sándor and Koncz, Anna and Németh, Krisztina and Lenzinger, Dorina and V. Vukman, Krisztina and Molnár, Kinga and Truszka, Mónika and Nakanishi, Hideyuki and Lőrincz, Péter and Székács, Inna and Buzás, Edit I. and Horváth, Róbert},
	unique-id = {34486423},
	year = {2023},
	orcid-numbers = {Visnovitz, Tamás/0000-0002-7962-5083; Kurunczi, Sándor/0000-0002-6567-5231; Lőrincz, Péter/0000-0001-7374-667X; Horváth, Róbert/0000-0001-8617-2302}
}

@{MTMT:34486404,
	title = {Nanoinjection of fluorescent nanoparticles to single live cells by robotic fluidic force microscopy},
	url = {https://m2.mtmt.hu/api/publication/34486404},
	author = {Kovács, Kinga Dóra and Visnovitz, Tamás and Gerecsei, Tamás and Péter, Beatrix and Kurunczi, Sándor and Koncz, Anna and Németh, Krisztina and Lenzinger, Dorina and V. Vukman, Krisztina and Lőrincz, Péter and Székács, Inna and Buzás, Edit I. and Horváth, Róbert},
	booktitle = {Magyar Biofizikai Társaság XXIX. Kongresszusa},
	unique-id = {34486404},
	year = {2023},
	pages = {35-35},
	orcid-numbers = {Visnovitz, Tamás/0000-0002-7962-5083; Kurunczi, Sándor/0000-0002-6567-5231; Lőrincz, Péter/0000-0001-7374-667X; Horváth, Róbert/0000-0001-8617-2302}
}

@article{MTMT:34425078,
	title = {Nanoinjection of extracellular vesicles to single live cells by robotic fluidic force microscopy},
	url = {https://m2.mtmt.hu/api/publication/34425078},
	author = {Kovács, Kinga Dóra and Visnovitz, Tamás and Gerecsei, Tamás and Péter, Beatrix and Kurunczi, Sándor and Koncz, Anna and Németh, Krisztina and Lenzinger, Dorina and Visnovitzné Dr Vukman, Krisztina and Balogh, Anna and Rajmon, Imola and Lőrincz, Péter and Székács, Inna and Buzás, Edit Irén and Horváth, Róbert},
	doi = {10.1002/jev2.12388},
	journal-iso = {J EXTRACELLULAR VESICL},
	journal = {JOURNAL OF EXTRACELLULAR VESICLES},
	volume = {12},
	unique-id = {34425078},
	abstract = {In the past decade, extracellular vesicles (EVs) have attracted substantial interest in biomedicine. With progress in the field, we have an increasing understanding of cellular responses to EVs. In this Technical Report, we describe the direct nanoinjection of EVs into the cytoplasm of single cells of different cell lines. By using robotic fluidic force microscopy (robotic FluidFM), nanoinjection of GFP positive EVs and EV‐like particles into single live HeLa, H9c2, MDA‐MB‐231 and LCLC‐103H cells proved to be feasible. This injection platform offered the advantage of high cell selectivity and efficiency. The nanoinjected EVs were initially localized in concentrated spot‐like regions within the cytoplasm. Later, they were transported towards the periphery of the cells. Based on our proof‐of‐principle data, robotic FluidFM is suitable for targeting single living cells by EVs and may lead to information about intracellular EV cargo delivery at a single‐cell level.},
	year = {2023},
	eissn = {2001-3078},
	orcid-numbers = {Visnovitz, Tamás/0000-0002-7962-5083; Kurunczi, Sándor/0000-0002-6567-5231; Koncz, Anna/0000-0003-2511-2394; Németh, Krisztina/0000-0002-3825-2137; Lőrincz, Péter/0000-0001-7374-667X; Buzás, Edit Irén/0000-0002-3744-206X; Horváth, Róbert/0000-0001-8617-2302}
}