TY - JOUR AU - Bod, Réka AU - Tóth, Kinga AU - Essam, Nour AU - Tóth, Estilla Zsófia AU - Erőss, Loránd AU - Entz, László AU - Bagó, Attila György AU - Fabó, Dániel AU - Ulbert, István AU - Wittner, Lucia TI - Synaptic alterations and neuronal firing in human epileptic neocortical excitatory networks JF - FRONTIERS IN SYNAPTIC NEUROSCIENCE J2 - FRONT SYNAPTIC NEURO VL - 15 PY - 2023 PG - 22 SN - 1663-3563 DO - 10.3389/fnsyn.2023.1233569 UR - https://m2.mtmt.hu/api/publication/34093583 ID - 34093583 N1 - eCollection AB - Epilepsy is a prevalent neurological condition, with underlying neuronal mechanisms involving hyperexcitability and hypersynchrony. Imbalance between excitatory and inhibitory circuits, as well as histological reorganization are relatively well-documented in animal models or even in the human hippocampus, but less is known about human neocortical epileptic activity. Our knowledge about changes in the excitatory signaling is especially scarce, compared to that about the inhibitory cell population. This study investigated the firing properties of single neurons in the human neocortex in vitro , during pharmacological blockade of glutamate receptors, and additionally evaluated anatomical changes in the excitatory circuit in tissue samples from epileptic and non-epileptic patients. Both epileptic and non-epileptic tissues exhibited spontaneous population activity (SPA), NMDA receptor antagonization reduced SPA recurrence only in epileptic tissue, whereas further blockade of AMPA/kainate receptors reversibly abolished SPA emergence regardless of epilepsy. Firing rates did not significantly change in excitatory principal cells and inhibitory interneurons during pharmacological experiments. Granular layer (L4) neurons showed an increased firing rate in epileptic compared to non-epileptic tissue. The burstiness of neurons remained unchanged, except for that of inhibitory cells in epileptic recordings, which decreased during blockade of glutamate receptors. Crosscorrelograms computed from single neuron discharge revealed both mono- and polysynaptic connections, particularly involving intrinsically bursting principal cells. Histological investigations found similar densities of SMI-32-immunopositive long-range projecting pyramidal cells in both groups, and shorter excitatory synaptic active zones with a higher proportion of perforated synapses in the epileptic group. These findings provide insights into epileptic modifications from the perspective of the excitatory system and highlight discrete alterations in firing patterns and synaptic structure. Our data suggest that NMDA-dependent glutamatergic signaling, as well as the excitatory synaptic machinery are perturbed in epilepsy, which might contribute to epileptic activity in the human neocortex. LA - English DB - MTMT ER - TY - CHAP AU - Entz, László AU - Erőss, Loránd ED - Banczerowski, Péter ED - Vajda, János TI - Pszichiátriai kórképek idegsebészeti kezelése T2 - Az idegsebészet alapjai PB - Medicina Könyvkiadó Zrt. CY - Budapest SN - 9789632268385 PY - 2022 SP - 521 EP - 523 PG - 3 UR - https://m2.mtmt.hu/api/publication/33194844 ID - 33194844 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Entz, László AU - Erőss, Loránd ED - Banczerowski, Péter ED - Vajda, János TI - A spaszticitás idegsebészeti kezelése T2 - Az idegsebészet alapjai PB - Medicina Könyvkiadó Zrt. CY - Budapest SN - 9789632268385 PY - 2022 SP - 517 EP - 521 PG - 5 UR - https://m2.mtmt.hu/api/publication/33194829 ID - 33194829 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Hajnal, Boglárka Zsófia AU - Entz, László AU - Tóth, Emília AU - Wittner, Lucia AU - Ulbert, István AU - Erőss, Loránd AU - Fabó, Dániel TI - A humán agykéreg elektromos ingerlésével kiváltott válaszok alatti lokális hálózati és egysejt-szintű aktivitásváltozásainak vizsgálata JF - IDEGGYÓGYÁSZATI SZEMLE PROCEEDINGS / CLINICAL NEUROSCIENCE PROCEEDINGS J2 - IDEGGYÓGY SZEMLE PROC VL - 7 PY - 2022 IS - 1 SP - 18 SN - 2498-6240 UR - https://m2.mtmt.hu/api/publication/33075628 ID - 33075628 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Hofer, Katharina AU - Kandrács, Ágnes AU - Tóth, Kinga AU - Hajnal, Boglárka Zsófia AU - Bokodi, Virág AU - Tóth, Estilla Zsófia AU - Erőss, Loránd AU - Entz, László AU - Bagó, Attila György AU - Fabó, Dániel AU - Ulbert, István AU - Wittner, Lucia TI - Bursting of excitatory cells is linked to interictal epileptic discharge generation in humans. JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 12 PY - 2022 IS - 1 PG - 17 SN - 2045-2322 DO - 10.1038/s41598-022-10319-4 UR - https://m2.mtmt.hu/api/publication/32785453 ID - 32785453 N1 - Funding Agency and Grant Number: ELKH Research Centre for Natural Sciences; Postdoctoral fellowship of the Hungarian Academy of Sciences; Hungarian Brain Research Program [KTIA_13_NAP-A-IV/1-4,6, KTIA 13 NAP-A-I/1, 2017-1.2.1-NKP-2017-00002, KTIA_NAP_13-1-2013-0001, KTIA-NAP17-3-2017-0001]; Hungarian National Research Fund [OTKA K119443, K137886, PD121123] Funding text: Open access funding provided by ELKH Research Centre for Natural Sciences. This study was supported by the Postdoctoral fellowship of the Hungarian Academy of Sciences (to K. T.), by the Hungarian Brain Research Program, KTIA_13_NAP-A-IV/1-4,6, KTIA 13 NAP-A-I/1 and 2017-1.2.1-NKP-2017-00002 (to I. U.) and KTIA_NAP_13-1-2013-0001, KTIA-NAP17-3-2017-0001 (to D. F.), and by the Hungarian National Research Fund OTKA K119443 and K137886 (to L. W.) and PD121123 (to K. T.) grants. AB - Knowledge about the activity of single neurons is essential in understanding the mechanisms of synchrony generation, and particularly interesting if related to pathological conditions. The generation of interictal spikes-the hypersynchronous events between seizures-is linked to hyperexcitability and to bursting behaviour of neurons in animal models. To explore its cellular mechanisms in humans we investigated the activity of clustered single neurons in a human in vitro model generating both physiological and epileptiform synchronous events. We show that non-epileptic synchronous events resulted from the finely balanced firing of excitatory and inhibitory cells, which was shifted towards an enhanced excitability in epileptic tissue. In contrast, interictal-like spikes were characterised by an asymmetric overall neuronal discharge initiated by excitatory neurons with the presumptive leading role of bursting pyramidal cells, and possibly terminated by inhibitory interneurons. We found that the overall burstiness of human neocortical neurons is not necessarily related to epilepsy, but the bursting behaviour of excitatory cells comprising both intrinsic and synaptically driven bursting is clearly linked to the generation of epileptiform synchrony. LA - English DB - MTMT ER - TY - JOUR AU - Hajnal, Boglárka Zsófia AU - Entz, László AU - Tóth, Emília AU - Ulbert, István AU - Erőss, Loránd AU - Fabó, Dániel TI - Local neuronal network and single unit changes evoked by single and paired pulse cortical stimulation in humans JF - EPILEPSIA J2 - EPILEPSIA VL - 62 PY - 2021 IS - Suppl. 3 SP - 264 EP - 265 PG - 2 SN - 0013-9580 UR - https://m2.mtmt.hu/api/publication/32548110 ID - 32548110 LA - English DB - MTMT ER - TY - GEN AU - Zsófia Tóth, Estilla AU - Erőss, Loránd AU - Entz, László AU - Bagó, Attila György AU - Fabó, Dániel AU - Ulbert, István AU - Wittner, Lucia AU - Tóth, Kinga TI - Alterations in perisomatic inhibition contributes to the higher synchrony in human epileptic neocortical tissues PY - 2020 UR - https://m2.mtmt.hu/api/publication/32173211 ID - 32173211 N1 - Poster LA - English DB - MTMT ER - TY - GEN AU - Szalárdy, Orsolya AU - Simor, Péter AU - Ujma, Przemyslaw Péter AU - Jordán, Zsófia AU - Borbély, Csaba AU - Halász, László AU - Erőss, Loránd AU - Entz, László AU - Halász, Péter AU - Fabó, Dániel AU - Bódizs, Róbert TI - Interictal epileptic discharges associated with sleep spindles in the anterior and mediodorsal thalamus PY - 2020 UR - https://m2.mtmt.hu/api/publication/32173181 ID - 32173181 N1 - Poster LA - English DB - MTMT ER - TY - JOUR AU - File, Bálint AU - Nánási, Tibor AU - Tóth, E. AU - Bokodi, Virág AU - Tóth, Brigitta AU - Hajnal, Boglárka Zsófia AU - Balogh-Kardos, Zsófia Klára AU - Entz, László AU - Erőss, Loránd AU - Ulbert, István AU - Fabó, Dániel TI - Reorganization of Large-Scale Functional Networks during Low-Frequency Electrical Stimulation of the Cortical Surface JF - INTERNATIONAL JOURNAL OF NEURAL SYSTEMS J2 - INT J NEURAL SYST VL - 30 PY - 2020 IS - 3 PG - 15 SN - 0129-0657 DO - 10.1142/S0129065719500229 UR - https://m2.mtmt.hu/api/publication/30868010 ID - 30868010 N1 - Funding Agency and Grant Number: Hungarian Brain Research Program Grants [2017-1.2.1-NKP-2017-00002]; European Social Fund [EFOP-3.6.3-VEKOP-16-2017-00002] Funding text: BF is supported by the Hungarian Brain Research Program Grants (Grant Nos. 2017-1.2.1-NKP-2017-00002) and the European Social Fund (EFOP-3.6.3-VEKOP-16-2017-00002). The authors are willing to share their data upon personal request. First and second authors contributed equally. LA - English DB - MTMT ER - TY - GEN AU - Barnaföldi, Luca AU - Halász, László AU - Emília, Tóth AU - Virág, Bokodi AU - Boglárka, Hajnal AU - Fabó, Dániel AU - Erőss, Loránd AU - Entz, László TI - Probabilistic tractography shows correlation with cortico-cortical evoked potentials (ccep) over ictal areas PY - 2019 UR - https://m2.mtmt.hu/api/publication/32235256 ID - 32235256 LA - English DB - MTMT ER -