TY - JOUR AU - Cinege, Gyöngyi Ilona AU - Zsámboki, János AU - Vidal-Quadras, M AU - Uv, A AU - Csordás, Gábor AU - Honti, Viktor AU - Gábor, Erika AU - Hegedűs, Zoltán AU - Varga, Gergely István AU - Kovács, Attila Lajos AU - Juhász, Gábor AU - Williams, MJ AU - Andó, István AU - Kurucz, Judit Éva TI - Genes encoding cuticular proteins are components of the Nimrod gene cluster in Drosophila. JF - INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY J2 - INSECT BIOCHEM MOLEC VL - 87 PY - 2017 SP - 45 EP - 54 PG - 10 SN - 0965-1748 DO - 10.1016/j.ibmb.2017.06.006 UR - https://m2.mtmt.hu/api/publication/3249679 ID - 3249679 N1 - Immunology Unit, Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, P.O.Box 521, Szeged, H-6701, Hungary Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SE-40530, Sweden Laboratory of Bioinformatics, Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, P.O.Box 521, Szeged, H-6701, Hungary Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary Department of Neuroscience, Functional Pharmacology, Uppsala University, Sweden Institution För Neurovetenskap BMC, Husargatan 3, Box 593 751 24, Uppsala, Sweden Cited By :10 Export Date: 7 November 2023 CODEN: IBMBE Correspondence Address: Kurucz, É.; Immunology Unit, P.O.Box 521, Hungary; email: kurucz.eva@brc.mta.hu AB - The Nimrod gene cluster, located on the second chromosome of Drosophila melanogaster, is the largest synthenic unit of the Drosophila genome. Nimrod genes show blood cell specific expression and code for phagocytosis receptors that play a major role in fruit fly innate immune functions. We previously identified three homologous genes (vajk-1, vajk-2 and vajk-3) located within the Nimrod cluster, which are unrelated to the Nimrod genes, but are homologous to a fourth gene (vajk-4) located outside the cluster. Here we show that, unlike the Nimrod candidates, the Vajk proteins are expressed in cuticular structures of the late embryo and the late pupa, indicating that they contribute to cuticular barrier functions. LA - English DB - MTMT ER - TY - JOUR AU - Márkus, Róbert AU - Lerner, Zita AU - Honti, Viktor AU - Csordás, Gábor AU - Zsámboki, János AU - Cinege, Gyöngyi Ilona AU - Párducz, Árpád AU - Lukacsovich, Tamás AU - Kurucz, Judit Éva AU - Andó, István TI - Multinucleated Giant Hemocytes Are Effector Cells in Cell-Mediated Immune Responses of Drosophila JF - JOURNAL OF INNATE IMMUNITY J2 - J INNATE IMMUN VL - 7 PY - 2015 IS - 4 SP - 340 EP - 353 PG - 14 SN - 1662-811X DO - 10.1159/000369618 UR - https://m2.mtmt.hu/api/publication/2853634 ID - 2853634 N1 - Megjegyzés-25258522 Hiányzó Jelleg: 'JOUR\n\nArticle' Admin megjegyzés-25258522 tblcategory: (Category) ('JOUR\n\nArticle') #Jelleg AB - We identified and characterized a so far unrecognized cell type, dubbed the multinucleated giant hemocyte (MGH), in the ananassae subgroup of Drosophilidae. Here, we describe the functional and ultrastructural characteristics of this novel blood cell type as well as its characterization with a set of discriminative immunological markers. MGHs are encapsulating cells that isolate and kill the parasite without melanization. They share some properties with but differ considerably from lamellocytes, the encapsulating cells of Drosophila melanogaster, the broadly used model organism in studies of innate immunity. MGHs are nonproliferative effector cells that are derived from phagocytic cells of the sessile tissue and the circulation, but do not exhibit phagocytic activity. In contrast to lamellocytes, MGHs are gigantic cells with filamentous projections and contain many nuclei, which are the result of the fusion of several cells. Although the structure of lamellocytes and MGHs differ remarkably, their function in the elimination of parasites is similar, which is potentially the result of the convergent evolution of interactions between hosts and parasites in different geographic regions. MGHs are highly motile and share several features with mammalian multinucleated giant cells, a syncytium of macrophages formed during granulomatous inflammation. © 2015 S. Karger AG, Basel LA - English DB - MTMT ER - TY - JOUR AU - Bretscher, AJ AU - Honti, Viktor AU - Binggeli, O AU - Burri, O AU - Poidevin, M AU - Kurucz, Judit Éva AU - Zsámboki, János AU - Andó, István AU - Lemaitre, B TI - The Nimrod transmembrane receptor Eater is required for hemocyte attachment to the sessile compartment in Drosophila melanogaster. JF - BIOLOGY OPEN J2 - BIOL OPEN VL - 4 PY - 2015 IS - 3 SP - 355 EP - 363 PG - 9 SN - 2046-6390 DO - 10.1242/bio.201410595 UR - https://m2.mtmt.hu/api/publication/2853633 ID - 2853633 AB - Eater is an EGF-like repeat transmembrane receptor of the Nimrod family and is expressed in Drosophila hemocytes. Eater was initially identified for its role in phagocytosis of both Gram-positive and Gram-negative bacteria. We have deleted eater and show that it appears to be required for efficient phagocytosis of Gram-positive but not Gram-negative bacteria. However, the most striking phenotype of eater deficient larvae is the near absence of sessile hemocytes, both plasmatocyte and crystal cell types. The eater deletion is the first loss of function mutation identified that causes absence of the sessile hemocyte state. Our study shows that Eater is required cell-autonomously in plasmatocytes for sessility. However, the presence of crystal cells in the sessile compartment requires Eater in plasmatocytes. We also show that eater deficient hemocytes exhibit a cell adhesion defect. Collectively, our data uncovers a new requirement of Eater in enabling hemocyte attachment at the sessile compartment and points to a possible role of Nimrod family members in hemocyte adhesion. LA - English DB - MTMT ER - TY - THES AU - Zsámboki, János TI - A Nimród fehérje- és géncsalád szerepe a mikroorganizmusok felismerésében és bekebelezésében PB - Szegedi Tudományegyetem (SZTE) PY - 2013 SP - 65 DO - 10.14232/phd.1879 UR - https://m2.mtmt.hu/api/publication/2890941 ID - 2890941 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Kari, Beáta AU - Zsámboki, János AU - Honti, Viktor AU - Csordás, Gábor AU - Márkus, Róbert AU - Andó, István AU - Kurucz, Judit Éva ED - Horváth, József ED - Monostori, Tamás TI - A SZEPTIKUS SÉRÜLÉST KÖVETŐ IMMUNVÁLASZT SZABÁLYOZÓ GENETIKAI FAKTOROK AZONOSÍTÁSÁNAK MÓDSZEREI DROSOPHILÁBAN T2 - Tudomány a vidék mindennapjaiban PB - Szegedi Tudományegyetem Mezőgazdasági Kar CY - Hódmezővásárhely SN - 9789633062456 PY - 2013 SP - 43 EP - 47 PG - 5 UR - https://m2.mtmt.hu/api/publication/2519814 ID - 2519814 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Gábor, Erika AU - Török, T AU - Csordás, Gábor AU - Zsámboki, János AU - Kurucz, Judit Éva AU - Andó, István ED - Horváth, József ED - Monostori, Tamás TI - A HÁZI MÉH (APIS MELLIFERA) IMMUNITÁSA T2 - Tudomány a vidék mindennapjaiban PB - Szegedi Tudományegyetem Mezőgazdasági Kar CY - Hódmezővásárhely SN - 9789633062456 PY - 2013 SP - 29 EP - 34 PG - 6 UR - https://m2.mtmt.hu/api/publication/2519812 ID - 2519812 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Zsámboki, János AU - Csordás, Gábor AU - Honti, Viktor AU - Andó, István AU - Kurucz, Judit Éva ED - Horváth, József ED - Monostori, Tamás TI - NIMRÓD FEHÉRJE- ÉS GÉNCSALÁD SZEREPE A MIKROORGANIZMUSOK FELISMERÉSÉBEN ÉS BEKEBELEZÉSÉBEN T2 - Tudomány a vidék mindennapjaiban PB - Szegedi Tudományegyetem Mezőgazdasági Kar CY - Hódmezővásárhely SN - 9789633062456 PY - 2013 SP - 80 EP - 84 PG - 5 UR - https://m2.mtmt.hu/api/publication/2519802 ID - 2519802 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Kari, Beáta AU - Zsámboki, János AU - Honti, Viktor AU - Csordás, Gábor AU - Márkus, Róbert AU - Andó, István AU - Kurucz, Judit Éva TI - A novel method for the identification of factors involved in host-pathogen interactions in Drosophila melanogaster. JF - JOURNAL OF IMMUNOLOGICAL METHODS J2 - J IMMUNOL METHODS VL - 398-399 PY - 2013 SP - 76 EP - 82 PG - 7 SN - 0022-1759 DO - 10.1016/j.jim.2013.09.011 UR - https://m2.mtmt.hu/api/publication/2463198 ID - 2463198 N1 - WoS:hiba:000329011500009 2019-03-03 20:54 kötet nem egyezik AB - A new method was established, standardized and validated for screening factors involved in the response to septic injury in Drosophila melanogaster. The method, based on inducing lesion by removing the tarsal segments of the first pair of legs of Drosophila adults and exposing them to different bacteria, imitates injury that often occurs in the natural habitat. The method is easy to perform, highly reproducible and suitable for large-scale genetic screens with the aim of identifying factors involved in host-pathogen interactions. The technique was validated by using mutant variations of different components of the immune response, blood clotting as well as the involvement of a number of genes known to be instrumental in the humoral and cell-mediated immune responses of Drosophila was confirmed. Moreover, the combination of the present method with antibiotic treatment allows the screening of potential antimicrobial drugs in vivo. LA - English DB - MTMT ER - TY - JOUR AU - Honti, Viktor AU - Cinege, Gyöngyi Ilona AU - Csordás, Gábor AU - Kurucz, Judit Éva AU - Zsámboki, János AU - Evans, CJ AU - Banerjee, U AU - Andó, István TI - Variation of NimC1 expression in Drosophila stocks and transgenic strains. JF - FLY J2 - FLY VL - 7 PY - 2013 IS - 4 SP - 263 EP - 266 PG - 4 SN - 1933-6934 DO - 10.4161/fly.25654 UR - https://m2.mtmt.hu/api/publication/2372552 ID - 2372552 AB - The NimC1 molecule has been described as a phagocytosis receptor, and is being used as a marker for professional phagocytes, the plasmatocytes, in Drosophila melanogaster. In studies including tumor-biology, developmental biology, and cell mediated immunity, monoclonal antibodies (P1a and P1b) to the NimC1 antigen are used. As we observed that these antibodies did not react with plasmatocytes of several strains and genetic combinations, a molecular analysis was performed on the structure of the nimC1 gene. We found 2 deletions and an insertion within the nimC1 gene, which may result in the production of a truncated NimC1 protein. The NimC1 positivity was regained by recombining the mutation with a wild-type allele or by using nimC1 mutant lines under heterozygous conditions. By means of these procedures or gaining access to the recombined stock, NimC1 can be used as a marker for phagocytic cells in the majority of the possible genetic backgrounds. LA - English DB - MTMT ER - TY - JOUR AU - Zsámboki, János AU - Csordás, Gábor AU - Honti, Viktor AU - Pintér, Lajos AU - Bajusz, Izabella AU - Galgóczi, László Norbert AU - Andó, István AU - Kurucz, Judit Éva TI - Drosophila Nimrod proteins bind bacteria JF - CENTRAL EUROPEAN JOURNAL OF BIOLOGY J2 - CENT EUR J BIOL VL - 8 PY - 2013 IS - 7 SP - 633 EP - 645 PG - 13 SN - 1895-104X DO - 10.2478/s11535-013-0183-4 UR - https://m2.mtmt.hu/api/publication/2326115 ID - 2326115 N1 - Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, H-6726 Szeged, Hungary Department of Microbiology, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Hungary Cited By :14 Export Date: 19 January 2022 Correspondence Address: Kurucz, É.; Institute of Genetics, , H-6726 Szeged, Hungary; email: kurucz.eva@brc.mta.hu AB - Engulfment of foreign particles by phagocytes is initiated by the engagement of phagocytic receptors. We have previously reported that NimC1 is involved in the phagocytosis of bacteria in Drosophila melanogaster. We have identified a family of genes, the Nimrod gene superfamily, encoding characteristic NIM domain containing structural homologues of NimC1. In this work we studied the bacterium-binding properties of the Nimrod proteins by using a novel immunofluorescencebased flow cytometric assay. This method proved to be highly reproducible and suitable for investigations of the bacteriumbinding capacities of putative phagocytosis receptors. We found that NimC1, NimA, NimB1 and NimB2 bind bacteria significantly but differently. In this respect they are similar to other NIM domain containing receptors Eater and Draper. LA - English DB - MTMT ER -