TY - JOUR AU - Ughy, Bettina AU - Nagyapáti, Sarolta AU - Lajkó, Dézi Bianka AU - Letoha, Tamas AU - Prohaszka, Adam AU - Deeb, Dima AU - Dér, András AU - Pettkó-Szandtner, Aladár AU - Szilák, László TI - Reconsidering Dogmas about the Growth of Bacterial Populations JF - CELLS J2 - CELLS-BASEL VL - 12 PY - 2023 IS - 10 PG - 15 SN - 2073-4409 DO - 10.3390/cells12101430 UR - https://m2.mtmt.hu/api/publication/33846356 ID - 33846356 N1 - Funding Agency and Grant Number: Hungarian Ministry for National Economy and National Research Development and Innovation Office of Hungary [GINOP-2.3.2-15-2016-00058]; European Union [101000501] Funding text: This research was funded by grants of the Hungarian Ministry for National Economy and National Research Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00058) and the European Unions Horizon 2020 Research and Innovation Program under grant agreement No 101000501. AB - The growth of bacterial populations has been described as a dynamic process of continuous reproduction and cell death. However, this is far from the reality. In a well fed, growing bacterial population, the stationary phase inevitably occurs, and it is not due to accumulated toxins or cell death. A population spends the most time in the stationary phase, where the phenotype of the cells alters from the proliferating ones, and only the colony forming unit (CFU) decreases after a while, not the total cell concentration. A bacterial population can be considered as a virtual tissue as a result of a specific differentiation process, in which the exponential-phase cells develop to stationary-phase cells and eventually reach the unculturable form. The richness of the nutrient had no effect on growth rate or on stationary cell density. The generation time seems not to be a constant value, but it depended on the concentration of the starter cultures. Inoculations with serial dilutions of stationary populations reveal a so-called minimal stationary cell concentration (MSCC) point, up to which the cell concentrations remain constant upon dilutions; that seems to be universal among unicellular organisms. LA - English DB - MTMT ER - TY - JOUR AU - Devadasu, Elsinraju Devadasu AU - Kanna, Sai Divya AU - Neelam, Satyabala AU - Yadav, Ranay Mohan AU - Nama, Srilatha AU - Akhtar, Parveen AU - Polgár, Tamás Ferenc AU - Ughy, Bettina AU - Garab, Győző AU - Lambrev, Petar AU - Subramanyam, Rajagopal TI - Long- and short-term acclimation of the photosynthetic apparatus to salinity in Chlamydomonas reinhardtii. The role of Stt7 protein kinase JF - FRONTIERS IN PLANT SCIENCE J2 - FRONT PLANT SCI VL - 14 PY - 2023 PG - 17 SN - 1664-462X DO - 10.3389/fpls.2023.1051711 UR - https://m2.mtmt.hu/api/publication/33775142 ID - 33775142 N1 - Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India Institute of Plant Biology, Biological Research Centre, Eötvös Loránd Research Network, Szeged, Hungary Doctoral School of Biology, University of Szeged, Szeged, Hungary Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India Institute of Biophysics, Biological Research Centre, Eötvös Loránd Research Network, Szeged, Hungary Theoretical Medicine Doctoral School, University of Szeged, Szeged, Hungary Department of Physics, Faculty of Science, University of Ostrava, Ostrava, Czech Republic Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, United States Export Date: 8 May 2023 Correspondence Address: Subramanyam, R.; Department of Plant Sciences, India; email: srgsl@uohyd.ernet.in Funding details: 3.6.3-VEKOP-16-2017-00009 Funding details: GINOP-2.3, GINOP-2.3.2-15-2016-00001 Funding details: UoH/IoE/RC1/RC1-20-019 Funding details: Horizon 2020 Framework Programme, H2020, 101000501 Funding details: Department of Biotechnology, Ministry of Science and Technology, India, DBT, BT/INF/22/SP41176/2020, BT/PR11277/GBD/27/152/2008, BT/PR15132/BRB/10/909/2011 Funding details: Department of Science and Technology, Ministry of Science and Technology, India, डीएसटी Funding details: Council of Scientific and Industrial Research, India, CSIR, 38 (1504)/21/EMR-ll Funding details: University Grants Commission, UGC Funding details: Science and Engineering Research Board, SERB, CRG/2020/000489 Funding details: Ministry of Finance, MOF Funding details: Ministry of Finance Japan, MOF Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFIH, K-128679, KH-124985, NN-124904 Funding details: Okayama University Funding details: National Research, Development and Innovation Office Funding text 1: This research was supported by the Department of Biotechnology (BT/PR11277/GBD/27/152/2008, No: BT/PR15132/BRB/10/909/2011) the Institute of Eminence (UoH/IoE/RC1/RC1-20-019), Council of Scientific and Industrial Research (No.38 (1504)/21/EMR-ll), Science and Engineering Research Board (CRG/2020/000489) and also Indo-Israel Joint Research Program by UGC (No. F 6-8/2018(IC)), DST-FIST, UGC-SAP, DBT-Builder (BT/INF/22/SP41176/2020), India. SBN and ED acknowledged RGNF for awarding fellowship in the form of JRF and SRF. This work was also supported by grants of the National Research Development and Innovation Office of Hungary (NKFI KH-124985, K-128679 to GG and NN-124904 to PL), the Hungarian Ministry for National Economy (GINOP-2.3.2-15-2016-00001 to PL and GINOP-2.3.-15-2016-00058 to BU), European Union’s Horizon 2020 Research and Innovation Programme (No. 101000501 to BU and SK). TP was supported by EFOP 3.6.3-VEKOP-16-2017-00009 with the financial aid of the Ministry of Finance, Hungary. We thank Yuichiro Takahashi, Okayama University, Japan for providing the mutant of stt7. Funding text 2: This research was supported by the Department of Biotechnology (BT/PR11277/GBD/27/152/2008, No: BT/PR15132/BRB/10/909/2011) the Institute of Eminence (UoH/IoE/RC1/RC1-20-019), Council of Scientific and Industrial Research (No.38 (1504)/21/EMR-ll), Science and Engineering Research Board (CRG/2020/000489) and also Indo-Israel Joint Research Program by UGC (No. F 6-8/2018(IC)), DST-FIST, UGC-SAP, DBT-Builder (BT/INF/22/SP41176/2020), India. SBN and ED acknowledged RGNF for awarding fellowship in the form of JRF and SRF. This work was also supported by grants of the National Research Development and Innovation Office of Hungary (NKFI KH-124985, K-128679 to GG and NN-124904 to PL), the Hungarian Ministry for National Economy (GINOP-2.3.2-15-2016-00001 to PL and GINOP-2.3.-15-2016-00058 to BU), European Union’s Horizon 2020 Research and Innovation Programme (No. 101000501 to BU and SK). TP was supported by EFOP 3.6.3-VEKOP-16-2017-00009 with the financial aid of the Ministry of Finance, Hungary. We thank Yuichiro Takahashi, Okayama University, Japan for providing the mutant of stt7. LA - English DB - MTMT ER - TY - JOUR AU - Dlouhy, Ondrej AU - Karlicky, Vaclav AU - Javornik, Uros AU - Kurasova, Irena AU - Zsíros, Ottó AU - Sket, Primoz AU - Kanna, Sai Divya AU - Böde, Kinga AU - Vecerova, Kristyna AU - Urban, Otmar AU - Gasanoff, Edward S. AU - Plavec, Janez AU - Spunda, Vladimir AU - Ughy, Bettina AU - Garab, Győző TI - Structural Entities Associated with Different Lipid Phases of Plant Thylakoid Membranes-Selective Susceptibilities to Different Lipases and Proteases JF - CELLS J2 - CELLS-BASEL VL - 11 PY - 2022 IS - 17 PG - 18 SN - 2073-4409 DO - 10.3390/cells11172681 UR - https://m2.mtmt.hu/api/publication/33117892 ID - 33117892 N1 - Funding Agency and Grant Number: Czech Science Foundation [GACR 19-13637S]; National Research Development and Innovation Office of Hungary [OTKA K 128679, GINOP-2.3.2-15-2016-00058]; Ministry of Education, Youth and Sports of the Czech Republic [CZ.02.1.01/0.0/0.0/16_019/0000797]; Silesian Region [07359/2019/RRC]; European Union [101000501]; University of Ostrava [SGS11/P.rF/2022]; Slovenian Research Agency (ARRS) [P1-242]; Central European Research Infrastructure (CERIC-ERIC) Consortium; Slovenian NMR Center of CERIC-ERIC Consortium at National Institute of Chemistry in Ljubljana [20217148] Funding text: This work was supported by grants from Czech Science Foundation (GACR 19-13637S, to G.G.), National Research Development and Innovation Office of Hungary (OTKA K 128679, to G.G., and GINOP-2.3.2-15-2016-00058, to B.U.), the Ministry of Education, Youth and Sports of the Czech Republic (project "SustES-Adaptation strategies for sustainable ecosystem services and food security under adverse environmental conditions"; CZ.02.1.01/0.0/0.0/16_019/0000797, to V.S., K.V., O.U., I.K., and V.K.), Silesian Region (07359/2019/RRC, to O.D.), European Union`s Horizon 2020 Research and Innovation Program (No. 101000501, to B.U. and S.D.K), University of Ostrava (SGS11/P.rF/2022, to O.D.) and Slovenian Research Agency (ARRS, grant P1-242 to J.P.). The authors acknowledge Central European Research Infrastructure (CERIC-ERIC) Consortium for the access to experimental facilities and financial support. The 31P-NMR measurements were performed in Slovenian NMR Center of CERIC-ERIC Consortium at National Institute of Chemistry in Ljubljana (project No. 20217148). AB - It is well established that plant thylakoid membranes (TMs), in addition to a bilayer, contain two isotropic lipid phases and an inverted hexagonal (H-II) phase. To elucidate the origin of non-bilayer lipid phases, we recorded the P-31-NMR spectra of isolated spinach plastoglobuli and TMs and tested their susceptibilities to lipases and proteases; the structural and functional characteristics of TMs were monitored using biophysical techniques and CN-PAGE. Phospholipase-A1 gradually destroyed all P-31-NMR-detectable lipid phases of isolated TMs, but the weak signal of isolated plastoglobuli was not affected. Parallel with the destabilization of their lamellar phase, TMs lost their impermeability; other effects, mainly on Photosystem-II, lagged behind the destruction of the original phases. Wheat-germ lipase selectively eliminated the isotropic phases but exerted little or no effect on the structural and functional parameters of TMs-indicating that the isotropic phases are located outside the protein-rich regions and might be involved in membrane fusion. Trypsin and Proteinase K selectively suppressed the H-II phase-suggesting that a large fraction of TM lipids encapsulate stroma-side proteins or polypeptides. We conclude that-in line with the Dynamic Exchange Model-the non-bilayer lipid phases of TMs are found in subdomains separated from but interconnected with the bilayer accommodating the main components of the photosynthetic machinery. LA - English DB - MTMT ER - TY - JOUR AU - Rantala, Marjaana AU - Ivanauskaite, Aiste AU - Laihonen, Laura AU - Kanna, Sai Divya AU - Ughy, Bettina AU - Mulo, Paula TI - Chloroplast Acetyltransferase GNAT2 is Involved in the Organization and Dynamics of Thylakoid Structure JF - PLANT AND CELL PHYSIOLOGY J2 - PLANT CELL PHYSIOL VL - 63 PY - 2022 IS - 9 SP - 1205 EP - 1214 PG - 10 SN - 0032-0781 DO - 10.1093/pcp/pcac096 UR - https://m2.mtmt.hu/api/publication/33063333 ID - 33063333 N1 - Funding Agency and Grant Number: Academy of Finland [307335, 321616]; Doctoral Programme in Molecular Life Sciences at the University of Turku; National Research Development and Innovation Office of Hungary [GINOP-2.3.2-15-2016-00058]; Turun Yliopistosaatio Funding text: Academy of Finland (307335, 321616 to M.R., A.I, L.L., P.M.); Doctoral Programme in Molecular Life Sciences at the University of Turku (to A.I.); Turun Yliopistosaa t io (to M.R. and L.L.); National Research Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00058 to B.U. and S.D.K.). AB - Higher plants acclimate to changes in light conditions by adjusting the thylakoid membrane ultrastructure. Additionally, excitation energy transfer between photosystem II (PSII) and photosystem I (PSI) is balanced in a process known as state transition. These modifications are mediated by reversible phosphorylation of Lhcb1 and Lhcb2 proteins in different pools of light-harvesting complex (LHCII) trimers. Our recent study demonstrated that chloroplast acetyltransferase NUCLEAR SHUTTLE INTERACTING (NSI)/GNAT2 (general control non-repressible 5 (GCN5)-related N-acetyltransferase 2) is also needed for the regulation of light harvesting, evidenced by the inability of the gnat2 mutant to perform state transitions although there are no defects in LHCII phosphorylation. Here, we show that despite contrasting phosphorylation states of LHCII, grana packing in the gnat2 and state transition 7 (stn7) mutants possesses similar features, as the thylakoid structure of the mutants does not respond to the shift from darkness to light, which is in striking contrast to wild type (Wt). Circular dichroism and native polyacrylamide gel electrophoresis analyses further revealed that the thylakoid protein complex organization of gnat2 and stn7 resembles each other, but differ from that of Wt. Also, the location of the phosphorylated Lhcb2 as well as the LHCII antenna within the thylakoid network in gnat2 mutant is different from that of Wt. In gnat2, the LHCII antenna remains largely in grana stacks, where the phosphorylated Lhcb2 is found in all LHCII trimer pools, including those associated with PSII. These results indicate that in addition to phosphorylation-mediated regulation through STN7, the GNAT2 enzyme is involved in the organization and dynamics of thylakoid structure, probably through the regulation of chloroplast protein acetylation. LA - English DB - MTMT ER - TY - CONF AU - Jakabné Sándor, Zsuzsanna AU - Ardó, László AU - Kanna, Sai Divya AU - Racskóné Domonkos, Ildikó AU - Ughy, Bettina AU - Perjéssy, Judit AU - Kóbori, Tímea Ottilia AU - Dergez, Ágnes Karolina TI - BIOACTIVE COMPOUNDS IN NEWLY ISOLATED FRESHWATER MICROALGAE STRAINS: IMPACT TO THE FLESH QUALITY OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) T2 - XX. TOWARDS PRECISION FISH NUTRITION AND FEEDING ( International Symposium on Fish Nutrition and Feeding PY - 2022 SP - 56 UR - https://m2.mtmt.hu/api/publication/32871973 ID - 32871973 LA - English DB - MTMT ER - TY - JOUR AU - Kanna, Sai Divya AU - Racskóné Domonkos, Ildikó AU - Kóbori, T.O. AU - Dergez, Ágnes Karolina AU - Böde, Kinga AU - Nagyapáti, Sarolta AU - Zsíros, Ottó AU - Ünnep, Renáta AU - Nagy, Gergely AU - Garab, Győző AU - Szilák, László AU - Solymosi, Katalin AU - Kovács, László AU - Ughy, Bettina TI - Salt Stress Induces Paramylon Accumulation and Fine-Tuning of the Macro-Organization of Thylakoid Membranes in Euglena gracilis Cells JF - FRONTIERS IN PLANT SCIENCE J2 - FRONT PLANT SCI VL - 12 PY - 2021 PG - 15 SN - 1664-462X DO - 10.3389/fpls.2021.725699 UR - https://m2.mtmt.hu/api/publication/32823174 ID - 32823174 LA - English DB - MTMT ER - TY - CONF AU - Jakabné Sándor, Zsuzsanna AU - Bíró, Janka AU - Kóbori, Tímea Ottilia AU - Dergez, Ágnes Karolina AU - Sai, Divya Kanna AU - Racskóné Domonkos, Ildikó AU - Ughy, Bettina AU - Ardó, László ED - European, Aquaculture Society TI - FIRST EXPERIENCE OF NEWLY ISOLATED FRESHWATER ALGAE STRAINS AS IMMUNSTIMULANTS FOR STERLET (ACIPENSER RUTHENUS) JUVENILES T2 - Aquaculture Europe 21 : Abstracts PB - European Aquaculture Society (EAS) C1 - Oostende PY - 2021 SP - 596 PG - 1 UR - https://m2.mtmt.hu/api/publication/32349113 ID - 32349113 LA - English DB - MTMT ER - TY - CONF AU - Ardó, László AU - Ughy, Bettina AU - Dergez, Ágnes Karolina AU - Kóbori, Tímea Ottilia AU - Jakabné Sándor, Zsuzsanna ED - European, Aquaculture Society TI - EFFECT OF NEWLY ISOLATED FRESHWATER MICROALGAE ON THE ACTIVITY OF HEAD KIDNEY LEUKOCYTES ISOLATED FROM PIKEPERCH (SANDER LUCIOPERCA ) AND COMMON CARP (CYPRINUS CARPIO) T2 - Aquaculture Europe 21 : Abstracts PB - European Aquaculture Society (EAS) C1 - Oostende PY - 2021 SP - 64 EP - 65 PG - 2 UR - https://m2.mtmt.hu/api/publication/32348396 ID - 32348396 LA - English DB - MTMT ER - TY - JOUR AU - Ardó, László AU - Berecz, Orsolya AU - Szűcs, Anita AU - Dergez, Ágnes Karolina AU - Ughy, Bettina AU - Kóbori, Tímea Ottilia AU - Jeney, Galina AU - Jakabné Sándor, Zsuzsanna TI - Egyes algaizolátumok immunstimuláló kapacitásának bemutatása JF - HALÁSZATFEJLESZTÉS J2 - HALÁSZATFEJLESZTÉS VL - 38 PY - 2021 SP - 67 EP - 68 PG - 2 SN - 9789632699615 SN - 1219-4816 UR - https://m2.mtmt.hu/api/publication/32225862 ID - 32225862 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Dergez, Ágnes Karolina AU - Koós, Ákos AU - Urbán, Gabriella AU - Kóbori, Tímea Ottilia AU - Ardó, László AU - Antal, Péter AU - Hajnal, Ferenc AU - Hatvani, Nóra AU - Jakabné Sándor, Zsuzsanna AU - Ughy, Bettina TI - Hazai és közép-európai algaizolátumok jellemzése és növekedésének optimalizálása laboratóriumi és pilot méretben JF - HALÁSZATFEJLESZTÉS J2 - HALÁSZATFEJLESZTÉS VL - 38 PY - 2021 SP - 64 EP - 65 PG - 2 SN - 9789632699615 SN - 1219-4816 UR - https://m2.mtmt.hu/api/publication/32225702 ID - 32225702 LA - Hungarian DB - MTMT ER -