TY - JOUR AU - Lima, Rui AU - Rathod, Balaji Baburao AU - Tiricz, Hilda AU - Howan, Dian Herlinda Octorina AU - Al Bouni, Mohamad Anas AU - Jenei, Sándor AU - Tímár, Edit AU - Endre, Gabriella AU - Tóth, Gábor AU - Kondorosi, Éva TI - Legume Plant Peptides as Sources of Novel Antimicrobial Molecules Against Human Pathogens JF - FRONTIERS IN MOLECULAR BIOSCIENCES J2 - FRONT MOL BIOSCI VL - 9 PY - 2022 PG - 12 SN - 2296-889X DO - 10.3389/fmolb.2022.870460 UR - https://m2.mtmt.hu/api/publication/32917591 ID - 32917591 N1 - Funding Agency and Grant Number: Hungarian National Office for Research, Development and Innovation (NKFIH) [KKP129924]; Balzan research grant Funding text: Research has been supported by the Hungarian National Office for Research, Development and Innovation (NKFIH) Frontline Research project KKP129924 and the Balzan research grant to EK. AB - Antimicrobial peptides are prominent components of the plant immune system acting against a wide variety of pathogens. Legume plants from the inverted repeat lacking clade (IRLC) have evolved a unique gene family encoding nodule-specific cysteine-rich NCR peptides acting in the symbiotic cells of root nodules, where they convert their bacterial endosymbionts into non-cultivable, polyploid nitrogen-fixing cells. NCRs are usually 30-50 amino acids long peptides having a characteristic pattern of 4 or 6 cysteines and highly divergent amino acid composition. While the function of NCRs is largely unknown, antimicrobial activity has been demonstrated for a few cationic Medicago truncatula NCR peptides against bacterial and fungal pathogens. The advantages of these plant peptides are their broad antimicrobial spectrum, fast killing modes of actions, multiple bacterial targets, and low propensity to develop resistance to them and no or low cytotoxicity to human cells. In the IRLC legumes, the number of NCR genes varies from a few to several hundred and it is possible that altogether hundreds of thousands of different NCR peptides exist. Due to the need for new antimicrobial agents, we investigated the antimicrobial potential of 104 synthetic NCR peptides from M. truncatula, M. sativa, Pisum sativum, Galega orientalis and Cicer arietinum against eight human pathogens, including ESKAPE bacteria. 50 NCRs showed antimicrobial activity with differences in the antimicrobial spectrum and effectivity. The most active peptides eliminated bacteria at concentrations from 0.8 to 3.1 mu M. High isoelectric point and positive net charge were important but not the only determinants of their antimicrobial activity. Testing the activity of shorter peptide derivatives against Acinetobacter baumannii and Candida albicans led to identification of regions responsible for the antimicrobial activity and provided insight into their potential modes of action. This work provides highly potent lead molecules without hemolytic activity on human blood cells for novel antimicrobial drugs to fight against pathogens. LA - English DB - MTMT ER - TY - JOUR AU - Szerencsés, Bettina AU - Gácser, Attila AU - Endre, Gabriella AU - Racskóné Domonkos, Ildikó AU - Tiricz, Hilda AU - Vágvölgyi, Csaba AU - Szolomájer, János AU - Howan, Dian Herlinda Octorina AU - Tóth, Gábor AU - Pfeiffer, Ilona AU - Kondorosi, Éva TI - Symbiotic NCR Peptide Fragments Affect the Viability, Morphology and Biofilm Formation of Candida Species JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 22 PY - 2021 IS - 7 PG - 20 SN - 1661-6596 DO - 10.3390/ijms22073666 UR - https://m2.mtmt.hu/api/publication/31953278 ID - 31953278 N1 - Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, H-6726, Hungary Biological Research Centre, Institute of Plant Biology, Szeged, H-6726, Hungary Department of Medical Chemistry, University of Szeged, Szeged, H-6720, Hungary MTA-SZTE Biomimetic Systems Research Group, University of Szeged, Szeged, H-6720, Hungary Export Date: 26 April 2021 Correspondence Address: Kondorosi, E.; Biological Research Centre, Hungary; email: eva.kondorosi@gmail.com Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, H-6726, Hungary Biological Research Centre, Institute of Plant Biology, Szeged, H-6726, Hungary Department of Medical Chemistry, University of Szeged, Szeged, H-6720, Hungary MTA-SZTE Biomimetic Systems Research Group, University of Szeged, Szeged, H-6720, Hungary Export Date: 1 May 2021 Correspondence Address: Kondorosi, E.; Biological Research Centre, Hungary; email: eva.kondorosi@gmail.com LA - English DB - MTMT ER - TY - JOUR AU - Jenei, Sándor AU - Tiricz, Hilda AU - Szolomájer, János AU - Tímár, Edit AU - Klement, Éva AU - Al Bouni, Mohamad Anas AU - Lima, Rui AU - Kata, Diána AU - Harmati, Mária AU - Buzás, Krisztina AU - Földesi, Imre AU - Tóth, Gábor AU - Endre, Gabriella AU - Kondorosi, Éva TI - Potent Chimeric Antimicrobial Derivatives of the Medicago truncatula NCR247 Symbiotic Peptide JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 11 PY - 2020 PG - 10 SN - 1664-302X DO - 10.3389/fmicb.2020.00270 UR - https://m2.mtmt.hu/api/publication/31281264 ID - 31281264 N1 - Funding Agency and Grant Number: Hungarian National Office for Research, Development and Innovation (NKFIH) [GINOP 2.3.2-15-2016-00014 Evomer, GINOP 2.3.2-15-2016-00015 I-KOM, GINOP-2.3.2-15-2016-00001, GINOP-2.3.2-15-2016-00020]; ERCEuropean Research Council (ERC) [269067]; NKFIH Frontline Research project [KKP129924]; Balzan research grant; [TUDFO/47138-1/2019-ITM FIKP]; [20391-3/2018/FEKUSTRAT] Funding text: Research has been supported by the Hungarian National Office for Research, Development and Innovation (NKFIH) through the grants GINOP 2.3.2-15-2016-00014 Evomer and GINOP 2.3.2-15-2016-00015 I-KOM, GINOP-2.3.2-15-2016-00001, GINOP-2.3.2-15-2016-00020; and by the ERC Advanced Grant 269067 "SymBiotics," the NKFIH Frontline Research project KKP129924 and the Balzan research grant to EKo; as well as by grants TUDFO/47138-1/2019-ITM FIKP and 20391-3/2018/FEKUSTRAT to GT. Institute of Plant Biology, Biological Research Centre, Szeged, Hungary Department of Medical Chemistry, University of Szeged, Szeged, Hungary Institute of Biochemistry, Biological Research Centre, Szeged, Hungary Department of Laboratory Medicine, University of Szeged, Szeged, Hungary Department of Oral Biology and Experimental Dental Research, University of Szeged, Szeged, Hungary MTA-SZTE Biomimetic Systems Research Group, University of Szeged, Szeged, Hungary Cited By :2 Export Date: 9 February 2021 Correspondence Address: Endre, G.; Institute of Plant Biology, Hungary; email: endre.gabriella@brc.hu Institute of Plant Biology, Biological Research Centre, Szeged, Hungary Department of Medical Chemistry, University of Szeged, Szeged, Hungary Institute of Biochemistry, Biological Research Centre, Szeged, Hungary Department of Laboratory Medicine, University of Szeged, Szeged, Hungary Department of Oral Biology and Experimental Dental Research, University of Szeged, Szeged, Hungary MTA-SZTE Biomimetic Systems Research Group, University of Szeged, Szeged, Hungary Cited By :3 Export Date: 1 May 2021 Correspondence Address: Endre, G.; Institute of Plant Biology, Hungary; email: endre.gabriella@brc.hu AB - In Rhizobium-legume symbiosis, the bacteria are converted into nitrogen-fixing bacteroids. In many legume species, differentiation of the endosymbiotic bacteria is irreversible, culminating in definitive loss of their cell division ability. This terminal differentiation is mediated by plant peptides produced in the symbiotic cells. In Medicago truncatula more than similar to 700 nodule-specific cysteine-rich (NCR) peptides are involved in this process. We have shown previously that NCR247 and NCR335 have strong antimicrobial activity on various pathogenic bacteria and identified interaction of NCR247 with many bacterial proteins, including FtsZ and several ribosomal proteins, which prevent bacterial cell division and protein synthesis. In this study we designed and synthetized various derivatives of NCR247, including shorter fragments and various chimeric derivatives. The antimicrobial activity of these peptides was tested on the ESKAPE bacteria; Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Escherichia coli as a member of Enterobacteriaceae and in addition Listeria monocytogenes and Salmonella enterica. The 12 amino acid long C-terminal half of NCR247, NCR247C partially retained the antimicrobial activity and preserved the multitarget interactions with partners of NCR247. Nevertheless NCR247C became ineffective on S. aureus, P. aeruginosa, and L. monocytogenes. The chimeric derivatives obtained by fusion of NCR247C with other peptide fragments and particularly with a truncated mastoparan sequence significantly increased bactericidal activity and altered the antimicrobial spectrum. The minimal bactericidal concentration of the most potent derivatives was 1.6 mu M, which is remarkably lower than that of most classical antibiotics. The killing activity of the NCR247-based chimeric peptides was practically instant. Importantly, these peptides had no hemolytic activity or cytotoxicity on human cells. The properties of these NCR derivatives make them promising antimicrobials for clinical use. LA - English DB - MTMT ER - TY - JOUR AU - Tiricz, Hilda AU - Nagy, Bettina AU - Ferenc, Györgyi AU - Török, Katalin AU - Nagy, István AU - Dudits, Dénes AU - Ayaydin, Ferhan TI - Relaxed chromatin induced by histone deacetylase inhibitors improves the oligonucleotide-directed gene editing in plant cells JF - JOURNAL OF PLANT RESEARCH J2 - J PLANT RES VL - 131 PY - 2018 IS - 1 SP - 179 EP - 189 PG - 11 SN - 0918-9440 DO - 10.1007/s10265-017-0975-8 UR - https://m2.mtmt.hu/api/publication/3252340 ID - 3252340 LA - English DB - MTMT ER - TY - JOUR AU - Balogh, Emese Petra AU - Mosolygó, Tímea AU - Tiricz, Hilda AU - Szabó, Ágnes Míra AU - Karai, Adrienn AU - Kerekes, Fanni AU - Virók, Dezső AU - Kondorosi, Éva AU - Burián, Katalin TI - Anti-chlamydial effect of plant peptides JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 61 PY - 2014 IS - 2 SP - 229 EP - 239 PG - 11 SN - 1217-8950 DO - 10.1556/AMicr.61.2014.2.12 UR - https://m2.mtmt.hu/api/publication/2590227 ID - 2590227 LA - English DB - MTMT ER - TY - THES AU - Tiricz, Hilda TI - Szimbiotikus eredetű, antimikrobiális típusú peptidek hatása Sinorhizobium meliloti baktériumra PB - Szegedi Tudományegyetem (SZTE) PY - 2013 SP - 88 DO - 10.14232/phd.1896 UR - https://m2.mtmt.hu/api/publication/2513953 ID - 2513953 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Tiricz, Hilda AU - Szűcs, Attila AU - Farkas, Attila AU - Pap, Bernadett AU - Lima, Rui AU - Maróti, Gergely AU - Kondorosi, Éva AU - Kereszt, Attila TI - Antimicrobial Nodule-Specific Cysteine-Rich Peptides Induce Membrane Depolarization-Associated Changes in the Transcriptome of Sinorhizobium meliloti. JF - APPLIED AND ENVIRONMENTAL MICROBIOLOGY J2 - APPL ENVIRON MICROB VL - 79 PY - 2013 IS - 21 SP - 6737 EP - 6746 PG - 10 SN - 0099-2240 DO - 10.1128/AEM.01791-13 UR - https://m2.mtmt.hu/api/publication/2448433 ID - 2448433 AB - Leguminous plants establish symbiosis with nitrogen-fixing alpha- and betaproteobacteria, collectively called rhizobia, which provide combined nitrogen to support plant growth. Members of the inverted repeat-lacking clade of legumes impose terminal differentiation on their endosymbiotic bacterium partners with the help of the nodule-specific cysteine-rich (NCR) peptide family composed of close to 600 members. Among the few tested NCR peptides, cationic ones had antirhizobial activity measured by reduction or elimination of the CFU and uptake of the membrane-impermeable dye propidium iodide. Here, the antimicrobial spectrum of two of these peptides, NCR247 and NCR335, was investigated, and their effect on the transcriptome of the natural target Sinorhizobium meliloti was characterized. Both peptides were able to kill quickly a wide range of Gram-negative and Gram-positive bacteria; however, their spectra were only partially overlapping, and differences were found also in their efficacy on given strains, indicating that the actions of NCR247 and NCR335 might be similar though not identical. Treatment of S. meliloti cultures with either peptide resulted in a quick downregulation of genes involved in basic cellular functions, such as transcription-translation and energy production, as well as upregulation of genes involved in stress and oxidative stress responses and membrane transport. Similar changes provoked mainly in Gram-positive bacteria by antimicrobial agents were coupled with the destruction of membrane potential, indicating that it might also be a common step in the bactericidal actions of NCR247 and NCR335. LA - English DB - MTMT ER - TY - CHAP AU - Wass-Matics, Heléna AU - Tiricz, Hilda AU - Horváth, Nikoletta AU - Biró, Borbála (Pacsutáné) ED - Bódi, Éva ED - Fekete, István ED - Kovács, Béla TI - Érzékeny és toleráns rhizobiumok szaporodása növekvő toxikus elemdózisok függvényében T2 - Fiatal kutatók az egészséges élelmiszerért PB - Debreceni Egyetem Agrár- és Gazdálkodástudományok Centruma CY - Debrecen SN - 9789634736011 PY - 2013 SP - 141 EP - 146 PG - 6 UR - https://m2.mtmt.hu/api/publication/2217437 ID - 2217437 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Kevei, Z AU - Baloban, M AU - Da, Ines O AU - Tiricz, Hilda AU - Kroll, A AU - Regulski, K AU - Mergaert, P AU - Kondorosi, Éva TI - Conserved CDC20 Cell Cycle Functions Are Carried out by Two of the Five Isoforms in Arabidopsis thaliana JF - PLOS ONE J2 - PLOS ONE VL - 6 PY - 2011 IS - 6 PG - 15 SN - 1932-6203 DO - 10.1371/journal.pone.0020618 UR - https://m2.mtmt.hu/api/publication/1921809 ID - 1921809 N1 - Cited By :50 Export Date: 18 August 2022 AB - Background: The CDC20 and Cdh1/CCS52 proteins are substrate determinants and activators of the Anaphase Promoting Complex/Cyclosome (APC/C) E3 ubiquitin ligase and as such they control the mitotic cell cycle by targeting the degradation of various cell cycle regulators. In yeasts and animals the main CDC20 function is the destruction of securin and mitotic cyclins. Plants have multiple CDC20 gene copies whose functions have not been explored yet. In Arabidopsis thaliana there are five CDC20 isoforms and here we aimed at defining their contribution to cell cycle regulation, substrate selectivity and plant development. Methodology/Principal Findings: Studying the gene structure and phylogeny of plant CDC20s, the expression of the five AtCDC20 gene copies and their interactions with the APC/C subunit APC10, the CCS52 proteins, components of the mitotic checkpoint complex (MCC) and mitotic cyclin substrates, conserved CDC20 functions could be assigned for AtCDC20.1 and AtCDC20.2. The other three intron-less genes were silent and specific for Arabidopsis. We show that AtCDC20.1 and AtCDC20.2 are components of the MCC and interact with mitotic cyclins with unexpected specificity. AtCDC20.1 and AtCDC20.2 are expressed in meristems, organ primordia and AtCDC20.1 also in pollen grains and developing seeds. Knocking down both genes simultaneously by RNAi resulted in severe delay in plant development and male sterility. In these lines, the meristem size was reduced while the cell size and ploidy levels were unaffected indicating that the lower cell number and likely slowdown of the cell cycle are the cause of reduced plant growth. Conclusions/Significance: The intron-containing CDC20 gene copies provide conserved and redundant functions for cell cycle progression in plants and are required for meristem maintenance, plant growth and male gametophyte formation. The Arabidopsis-specific intron-less genes are possibly "retrogenes" and have hitherto undefined functions or are pseudogenes. LA - English DB - MTMT ER - TY - JOUR AU - W, Van de Velde AU - G, Zehirov AU - Szatmári, Ágnes AU - Debreczeny, Mónika AU - H, Ishihara AU - Kevei, Zoltán AU - Farkas, Attila AU - Mikuláss, Kata AU - Nagy, Andrea AU - Tiricz, Hilda AU - B, Satiat-Jeunemaître AU - B, Alunni AU - M, Bourge AU - K, -i Kucho AU - M, Abe AU - Kereszt, Attila AU - Maróti, Gergely AU - T, Uchiumi AU - Kondorosi, Éva AU - P, Mergaert TI - Plant peptides govern terminal differentiation of bacteria in symbiosis JF - SCIENCE J2 - SCIENCE VL - 327 PY - 2010 IS - 5969 SP - 1122 EP - 1126 PG - 5 SN - 0036-8075 DO - 10.1126/science.1184057 UR - https://m2.mtmt.hu/api/publication/1378519 ID - 1378519 N1 - Institut des Sciences du Végétal, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette Cedex, France Graduate School of Science and Engineering, Kagoshima University, 890 0065 Kagoshima, Japan Plant Protection Institute, Hungarian Academy of Sciences, 1022 Budapest, Hungary Institute for Plant Genomics, Human Biotechnology and Bioenergy, Bay Zoltan Foundation for Applied Research, 6726 Szeged, Hungary Cited By :339 Export Date: 30 April 2021 CODEN: SCIEA Correspondence Address: Kondorosi, E.; Institut des Sciences du Végétal, , 91198 Gif-sur-Yvette Cedex, France; email: eva.kondorosi@isv.cnrs-gif.fr AB - Legume plants host nitrogen-fixing endosymbiotic Rhizobium bacteria in root nodules. In Medicaga truncatula, the bacteria undergo an irreversible (terminal) differentiation mediated by hitherto unidentified plant factors. We demonstrated that these factors are nodule-specific cysteine-rich (NCR) peptides that are targeted to the bacteria and enter the bacterial membrane and cytosol. Obstruction of NCR transport in the dnf1-1 signal peptidase mutant correlated with the absence of terminal bacterial differentiation. On the contrary, ectopic expression of NCRs in legumes devoid of NCRs or challenge of cultured rhizobia with peptides provoked symptoms of terminal differentiation. Because NCRs resemble antimicrobial peptides, our findings reveal a previously unknown innovation of the host plant, which adopts effectors of the innate immune system for symbiosis to manipulate the cell fate of endosymbiotic bacteria. LA - English DB - MTMT ER -