@article{MTMT:32917591,
	title = {Legume Plant Peptides as Sources of Novel Antimicrobial Molecules Against Human Pathogens},
	url = {https://m2.mtmt.hu/api/publication/32917591},
	author = {Lima, Rui and Rathod, Balaji Baburao and Tiricz, Hilda and Howan, Dian Herlinda Octorina and Al Bouni, Mohamad Anas and Jenei, Sándor and Tímár, Edit and Endre, Gabriella and Tóth, Gábor and Kondorosi, Éva},
	doi = {10.3389/fmolb.2022.870460},
	journal-iso = {FRONT MOL BIOSCI},
	journal = {FRONTIERS IN MOLECULAR BIOSCIENCES},
	volume = {9},
	unique-id = {32917591},
	abstract = {Antimicrobial peptides are prominent components of the plant immune system acting against a wide variety of pathogens. Legume plants from the inverted repeat lacking clade (IRLC) have evolved a unique gene family encoding nodule-specific cysteine-rich NCR peptides acting in the symbiotic cells of root nodules, where they convert their bacterial endosymbionts into non-cultivable, polyploid nitrogen-fixing cells. NCRs are usually 30-50 amino acids long peptides having a characteristic pattern of 4 or 6 cysteines and highly divergent amino acid composition. While the function of NCRs is largely unknown, antimicrobial activity has been demonstrated for a few cationic Medicago truncatula NCR peptides against bacterial and fungal pathogens. The advantages of these plant peptides are their broad antimicrobial spectrum, fast killing modes of actions, multiple bacterial targets, and low propensity to develop resistance to them and no or low cytotoxicity to human cells. In the IRLC legumes, the number of NCR genes varies from a few to several hundred and it is possible that altogether hundreds of thousands of different NCR peptides exist. Due to the need for new antimicrobial agents, we investigated the antimicrobial potential of 104 synthetic NCR peptides from M. truncatula, M. sativa, Pisum sativum, Galega orientalis and Cicer arietinum against eight human pathogens, including ESKAPE bacteria. 50 NCRs showed antimicrobial activity with differences in the antimicrobial spectrum and effectivity. The most active peptides eliminated bacteria at concentrations from 0.8 to 3.1 mu M. High isoelectric point and positive net charge were important but not the only determinants of their antimicrobial activity. Testing the activity of shorter peptide derivatives against Acinetobacter baumannii and Candida albicans led to identification of regions responsible for the antimicrobial activity and provided insight into their potential modes of action. This work provides highly potent lead molecules without hemolytic activity on human blood cells for novel antimicrobial drugs to fight against pathogens.},
	keywords = {DIFFERENTIATION; ANTIMICROBIAL ACTIVITY; Candida albicans; MEDICAGO-TRUNCATULA; Acinetobacter baumannii; CYSTEINE-RICH PEPTIDES; ESKAPE pathogens; legume NCR peptides},
	year = {2022},
	eissn = {2296-889X},
	orcid-numbers = {Tóth, Gábor/0000-0002-3604-4385; Kondorosi, Éva/0000-0002-4065-8515}
}

@article{MTMT:31953278,
	title = {Symbiotic NCR Peptide Fragments Affect the Viability, Morphology and Biofilm Formation of Candida Species},
	url = {https://m2.mtmt.hu/api/publication/31953278},
	author = {Szerencsés, Bettina and Gácser, Attila and Endre, Gabriella and Racskóné Domonkos, Ildikó and Tiricz, Hilda and Vágvölgyi, Csaba and Szolomájer, János and Howan, Dian Herlinda Octorina and Tóth, Gábor and Pfeiffer, Ilona and Kondorosi, Éva},
	doi = {10.3390/ijms22073666},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {22},
	unique-id = {31953278},
	issn = {1661-6596},
	year = {2021},
	eissn = {1422-0067},
	orcid-numbers = {Gácser, Attila/0000-0003-2939-9580; Vágvölgyi, Csaba/0000-0003-0009-7773; Szolomájer, János/0000-0003-1458-6156; Tóth, Gábor/0000-0002-3604-4385; Pfeiffer, Ilona/0000-0003-0680-7596; Kondorosi, Éva/0000-0002-4065-8515}
}

@article{MTMT:31281264,
	title = {Potent Chimeric Antimicrobial Derivatives of the Medicago truncatula NCR247 Symbiotic Peptide},
	url = {https://m2.mtmt.hu/api/publication/31281264},
	author = {Jenei, Sándor and Tiricz, Hilda and Szolomájer, János and Tímár, Edit and Klement, Éva and Al Bouni, Mohamad Anas and Lima, Rui and Kata, Diána and Harmati, Mária and Buzás, Krisztina and Földesi, Imre and Tóth, Gábor and Endre, Gabriella and Kondorosi, Éva},
	doi = {10.3389/fmicb.2020.00270},
	journal-iso = {FRONT MICROBIOL},
	journal = {FRONTIERS IN MICROBIOLOGY},
	volume = {11},
	unique-id = {31281264},
	issn = {1664-302X},
	abstract = {In Rhizobium-legume symbiosis, the bacteria are converted into nitrogen-fixing bacteroids. In many legume species, differentiation of the endosymbiotic bacteria is irreversible, culminating in definitive loss of their cell division ability. This terminal differentiation is mediated by plant peptides produced in the symbiotic cells. In Medicago truncatula more than similar to 700 nodule-specific cysteine-rich (NCR) peptides are involved in this process. We have shown previously that NCR247 and NCR335 have strong antimicrobial activity on various pathogenic bacteria and identified interaction of NCR247 with many bacterial proteins, including FtsZ and several ribosomal proteins, which prevent bacterial cell division and protein synthesis. In this study we designed and synthetized various derivatives of NCR247, including shorter fragments and various chimeric derivatives. The antimicrobial activity of these peptides was tested on the ESKAPE bacteria; Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Escherichia coli as a member of Enterobacteriaceae and in addition Listeria monocytogenes and Salmonella enterica. The 12 amino acid long C-terminal half of NCR247, NCR247C partially retained the antimicrobial activity and preserved the multitarget interactions with partners of NCR247. Nevertheless NCR247C became ineffective on S. aureus, P. aeruginosa, and L. monocytogenes. The chimeric derivatives obtained by fusion of NCR247C with other peptide fragments and particularly with a truncated mastoparan sequence significantly increased bactericidal activity and altered the antimicrobial spectrum. The minimal bactericidal concentration of the most potent derivatives was 1.6 mu M, which is remarkably lower than that of most classical antibiotics. The killing activity of the NCR247-based chimeric peptides was practically instant. Importantly, these peptides had no hemolytic activity or cytotoxicity on human cells. The properties of these NCR derivatives make them promising antimicrobials for clinical use.},
	keywords = {DIFFERENTIATION; ANTIMICROBIAL PEPTIDES; CELL-CYCLE; plant symbiotic nodule-specific cysteine-rich peptides; NCR247; ESKAPE bacteria; modes of antimicrobial activity; killing kinetics; bacterial targets},
	year = {2020},
	eissn = {1664-302X},
	orcid-numbers = {Szolomájer, János/0000-0003-1458-6156; Kata, Diána/0000-0002-4432-9380; Harmati, Mária/0000-0002-4875-5723; Buzás, Krisztina/0000-0001-8933-2033; Földesi, Imre/0000-0002-3329-8136; Tóth, Gábor/0000-0002-3604-4385; Kondorosi, Éva/0000-0002-4065-8515}
}

@article{MTMT:3252340,
	title = {Relaxed chromatin induced by histone deacetylase inhibitors improves the oligonucleotide-directed gene editing in plant cells},
	url = {https://m2.mtmt.hu/api/publication/3252340},
	author = {Tiricz, Hilda and Nagy, Bettina and Ferenc, Györgyi and Török, Katalin and Nagy, István and Dudits, Dénes and Ayaydin, Ferhan},
	doi = {10.1007/s10265-017-0975-8},
	journal-iso = {J PLANT RES},
	journal = {JOURNAL OF PLANT RESEARCH},
	volume = {131},
	unique-id = {3252340},
	issn = {0918-9440},
	year = {2018},
	eissn = {1618-0860},
	pages = {179-189},
	orcid-numbers = {Ferenc, Györgyi/0000-0002-3456-319X}
}

@article{MTMT:2590227,
	title = {Anti-chlamydial effect of plant peptides},
	url = {https://m2.mtmt.hu/api/publication/2590227},
	author = {Balogh, Emese Petra and Mosolygó, Tímea and Tiricz, Hilda and Szabó, Ágnes Míra and Karai, Adrienn and Kerekes, Fanni and Virók, Dezső and Kondorosi, Éva and Burián, Katalin},
	doi = {10.1556/AMicr.61.2014.2.12},
	journal-iso = {ACTA MICROBIOL IMMUNOL HUNG},
	journal = {ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA},
	volume = {61},
	unique-id = {2590227},
	issn = {1217-8950},
	keywords = {Humans; ARTICLE; PLANT; Plant Proteins; Molecular Sequence Data; amino acid sequence; vegetable protein; controlled study; nonhuman; drug effect; Protein Binding; in vitro study; concentration response; Mass spectrometry; Hela Cells; Microbial Sensitivity Tests; unclassified drug; drug protein binding; ANTIMICROBIAL ACTIVITY; antibacterial activity; bactericidal activity; ligand binding; Chaperonin 60; Bacterial Proteins; drug potency; barrel medic; Medicago truncatula; Chlamydia; Antimicrobial; polypeptide antibiotic agent; Chlamydia trachomatis; Antimicrobial Cationic Peptides; endosymbiont; HeLa cell line; ncr280 peptide; ncr247 peptide; ncr192 peptide; ncr183 peptide; ncr169 peptide; ncr168 peptide; ncr137 peptide; ncr095 peptide; ncr055 peptide; ncr044 peptide; ncr030 peptide; NCR peptide},
	year = {2014},
	eissn = {1588-2640},
	pages = {229-239},
	orcid-numbers = {Mosolygó, Tímea/0000-0002-4499-388X; Kondorosi, Éva/0000-0002-4065-8515; Burián, Katalin/0000-0003-1300-2374}
}

@mastersthesis{MTMT:2513953,
	title = {Szimbiotikus eredetű, antimikrobiális típusú peptidek hatása Sinorhizobium meliloti baktériumra},
	url = {https://m2.mtmt.hu/api/publication/2513953},
	author = {Tiricz, Hilda},
	doi = {10.14232/phd.1896},
	publisher = {SZTE},
	unique-id = {2513953},
	year = {2013}
}

@article{MTMT:2448433,
	title = {Antimicrobial Nodule-Specific Cysteine-Rich Peptides Induce Membrane Depolarization-Associated Changes in the Transcriptome of Sinorhizobium meliloti.},
	url = {https://m2.mtmt.hu/api/publication/2448433},
	author = {Tiricz, Hilda and Szűcs, Attila and Farkas, Attila and Pap, Bernadett and Lima, Rui and Maróti, Gergely and Kondorosi, Éva and Kereszt, Attila},
	doi = {10.1128/AEM.01791-13},
	journal-iso = {APPL ENVIRON MICROB},
	journal = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
	volume = {79},
	unique-id = {2448433},
	issn = {0099-2240},
	abstract = {Leguminous plants establish symbiosis with nitrogen-fixing alpha- and betaproteobacteria, collectively called rhizobia, which provide combined nitrogen to support plant growth. Members of the inverted repeat-lacking clade of legumes impose terminal differentiation on their endosymbiotic bacterium partners with the help of the nodule-specific cysteine-rich (NCR) peptide family composed of close to 600 members. Among the few tested NCR peptides, cationic ones had antirhizobial activity measured by reduction or elimination of the CFU and uptake of the membrane-impermeable dye propidium iodide. Here, the antimicrobial spectrum of two of these peptides, NCR247 and NCR335, was investigated, and their effect on the transcriptome of the natural target Sinorhizobium meliloti was characterized. Both peptides were able to kill quickly a wide range of Gram-negative and Gram-positive bacteria; however, their spectra were only partially overlapping, and differences were found also in their efficacy on given strains, indicating that the actions of NCR247 and NCR335 might be similar though not identical. Treatment of S. meliloti cultures with either peptide resulted in a quick downregulation of genes involved in basic cellular functions, such as transcription-translation and energy production, as well as upregulation of genes involved in stress and oxidative stress responses and membrane transport. Similar changes provoked mainly in Gram-positive bacteria by antimicrobial agents were coupled with the destruction of membrane potential, indicating that it might also be a common step in the bactericidal actions of NCR247 and NCR335.},
	year = {2013},
	eissn = {1098-5336},
	pages = {6737-6746},
	orcid-numbers = {Szűcs, Attila/0000-0003-2803-7123; Maróti, Gergely/0000-0002-3705-0461; Kondorosi, Éva/0000-0002-4065-8515}
}

@inproceedings{MTMT:2217437,
	title = {Érzékeny és toleráns rhizobiumok szaporodása növekvő toxikus elemdózisok függvényében},
	url = {https://m2.mtmt.hu/api/publication/2217437},
	author = {Wass-Matics, Heléna and Tiricz, Hilda and Horváth, Nikoletta and Biró, Borbála (Pacsutáné)},
	booktitle = {Fiatal kutatók az egészséges élelmiszerért},
	unique-id = {2217437},
	year = {2013},
	pages = {141-146}
}

@article{MTMT:1921809,
	title = {Conserved CDC20 Cell Cycle Functions Are Carried out by Two of the Five Isoforms in Arabidopsis thaliana},
	url = {https://m2.mtmt.hu/api/publication/1921809},
	author = {Kevei, Z and Baloban, M and Da, Ines O and Tiricz, Hilda and Kroll, A and Regulski, K and Mergaert, P and Kondorosi, Éva},
	doi = {10.1371/journal.pone.0020618},
	journal-iso = {PLOS ONE},
	journal = {PLOS ONE},
	volume = {6},
	unique-id = {1921809},
	issn = {1932-6203},
	abstract = {Background: The CDC20 and Cdh1/CCS52 proteins are substrate determinants and activators of the Anaphase Promoting Complex/Cyclosome (APC/C) E3 ubiquitin ligase and as such they control the mitotic cell cycle by targeting the degradation of various cell cycle regulators. In yeasts and animals the main CDC20 function is the destruction of securin and mitotic cyclins. Plants have multiple CDC20 gene copies whose functions have not been explored yet. In Arabidopsis thaliana there are five CDC20 isoforms and here we aimed at defining their contribution to cell cycle regulation, substrate selectivity and plant development. Methodology/Principal Findings: Studying the gene structure and phylogeny of plant CDC20s, the expression of the five AtCDC20 gene copies and their interactions with the APC/C subunit APC10, the CCS52 proteins, components of the mitotic checkpoint complex (MCC) and mitotic cyclin substrates, conserved CDC20 functions could be assigned for AtCDC20.1 and AtCDC20.2. The other three intron-less genes were silent and specific for Arabidopsis. We show that AtCDC20.1 and AtCDC20.2 are components of the MCC and interact with mitotic cyclins with unexpected specificity. AtCDC20.1 and AtCDC20.2 are expressed in meristems, organ primordia and AtCDC20.1 also in pollen grains and developing seeds. Knocking down both genes simultaneously by RNAi resulted in severe delay in plant development and male sterility. In these lines, the meristem size was reduced while the cell size and ploidy levels were unaffected indicating that the lower cell number and likely slowdown of the cell cycle are the cause of reduced plant growth. Conclusions/Significance: The intron-containing CDC20 gene copies provide conserved and redundant functions for cell cycle progression in plants and are required for meristem maintenance, plant growth and male gametophyte formation. The Arabidopsis-specific intron-less genes are possibly "retrogenes" and have hitherto undefined functions or are pseudogenes.},
	keywords = {metabolism; GENETICS; PROTEIN ISOFORMS; ARTICLE; PHYLOGENY; Chemistry; Protein Transport; POLLEN; PLANT DEVELOPMENT; Cell Count; growth, development and aging; Gene Expression Regulation, Plant; gene expression regulation; Molecular Sequence Data; molecular genetics; amino acid sequence; controlled study; Cytology; nonhuman; Animalia; Mitosis; Cell Cycle; Cell Cycle Proteins; RNA Interference; ANAPHASE-PROMOTING COMPLEX; Cell Size; Down-Regulation; ARABIDOPSIS; PROTEIN FUNCTION; protein expression; PSEUDOGENE; intracellular space; intron; down regulation; gene silencing; genetic conservation; protein interaction; isoprotein; gene structure; Arabidopsis thaliana; cycline; ploidy; plant growth; Arabidopsis Proteins; Arabidopsis protein; Cyclins; cyclin B1; cell cycle progression; plant gene; ubiquitin protein ligase; cell cycle protein; anaphase promoting complex; cell cycle regulation; meristem; primordium; seed development; gametophyte; Ubiquitin-Protein Ligase Complexes; cyclin A1; plant infertility; retrogene; male sterility; CDC20 protein, Arabidopsis; cyclin B2; cell cycle protein 20},
	year = {2011},
	eissn = {1932-6203},
	orcid-numbers = {Kondorosi, Éva/0000-0002-4065-8515}
}

@article{MTMT:1378519,
	title = {Plant peptides govern terminal differentiation of bacteria in symbiosis},
	url = {https://m2.mtmt.hu/api/publication/1378519},
	author = {W, Van de Velde and G, Zehirov and Szatmári, Ágnes and Debreczeny, Mónika and H, Ishihara and Kevei, Zoltán and Farkas, Attila and Mikuláss, Kata and Nagy, Andrea and Tiricz, Hilda and B, Satiat-Jeunemaître and B, Alunni and M, Bourge and K, -i Kucho and M, Abe and Kereszt, Attila and Maróti, Gergely and T, Uchiumi and Kondorosi, Éva and P, Mergaert},
	doi = {10.1126/science.1184057},
	journal-iso = {SCIENCE},
	journal = {SCIENCE},
	volume = {327},
	unique-id = {1378519},
	issn = {0036-8075},
	abstract = {Legume plants host nitrogen-fixing endosymbiotic Rhizobium bacteria in root nodules. In Medicaga truncatula, the bacteria undergo an irreversible (terminal) differentiation mediated by hitherto unidentified plant factors. We demonstrated that these factors are nodule-specific cysteine-rich (NCR) peptides that are targeted to the bacteria and enter the bacterial membrane and cytosol. Obstruction of NCR transport in the dnf1-1 signal peptidase mutant correlated with the absence of terminal bacterial differentiation. On the contrary, ectopic expression of NCRs in legumes devoid of NCRs or challenge of cultured rhizobia with peptides provoked symptoms of terminal differentiation. Because NCRs resemble antimicrobial peptides, our findings reveal a previously unknown innovation of the host plant, which adopts effectors of the innate immune system for symbiosis to manipulate the cell fate of endosymbiotic bacteria.},
	year = {2010},
	eissn = {1095-9203},
	pages = {1122-1126},
	orcid-numbers = {Szatmári, Ágnes/0000-0001-8768-8212; Maróti, Gergely/0000-0002-3705-0461; Kondorosi, Éva/0000-0002-4065-8515}
}