@article{MTMT:35082092,
	title = {A Comprehensive Study of Light Quality Acclimation in Synechocystis Sp. PCC 6803},
	url = {https://m2.mtmt.hu/api/publication/35082092},
	author = {Zavřel, Tomáš and Segečová, Anna and Kovács, László and Lukeš, Martin and Novák, Zoltán and Pohland, Anne-Christin and Szabó, Milán and Somogyi, Boglárka and Prášil, Ondřej and Červený, Jan and Bernát, Gábor},
	doi = {10.1093/pcp/pcae062},
	journal-iso = {PLANT CELL PHYSIOL},
	journal = {PLANT AND CELL PHYSIOLOGY},
	volume = {65},
	unique-id = {35082092},
	issn = {0032-0781},
	abstract = {Cyanobacteria play a key role in primary production in both oceans and fresh waters and hold great potential for sustainable production of a large number of commodities. During their life, cyanobacteria cells need to acclimate to a multitude of challenges, including shifts in intensity and quality of incident light. Despite our increasing understanding of metabolic regulation under various light regimes, detailed insight into fitness advantages and limitations under shifting light quality remains underexplored. Here, we study photo-physiological acclimation in the cyanobacterium Synechocystis sp. PCC 6803 throughout the photosynthetically active radiation (PAR) range. Using light emitting diodes (LEDs) with qualitatively different narrow spectra, we describe wavelength dependence of light capture, electron transport and energy transduction to main cellular pools. In addition, we describe processes that fine-tune light capture, such as state transitions, or the efficiency of energy transfer from phycobilisomes to photosystems (PS). We show that growth was the most limited under blue light due to inefficient light harvesting, and that many cellular processes are tightly linked to the redox state of the plastoquinone (PQ) pool, which was the most reduced under red light. The PSI-to-PSII ratio was low under blue photons, however, it was not the main growth-limiting factor, since it was even more reduced under violet and near far-red lights, where Synechocystis grew faster compared to blue light. Our results provide insight into the spectral dependence of phototrophic growth and can provide the foundation for future studies of molecular mechanisms underlying light acclimation in cyanobacteria, leading to light optimization in controlled cultivations.},
	year = {2024},
	eissn = {1471-9053},
	pages = {1285-1297},
	orcid-numbers = {Lukeš, Martin/0000-0003-3493-9780; Prášil, Ondřej/0000-0002-0012-4359; Bernát, Gábor/0000-0001-6147-9391}
}

@article{MTMT:34473960,
	title = {Monitoring the photosynthetic activity at single-cell level in Haematococcus lacustris},
	url = {https://m2.mtmt.hu/api/publication/34473960},
	author = {Patil, Priyanka Pradeep and Nagy, Krisztina and Ábrahám, Ágnes and Vass, Imre and Szabó, Milán},
	doi = {10.32615/ps.2023.042},
	journal-iso = {PHOTOSYNTHETICA},
	journal = {PHOTOSYNTHETICA},
	volume = {61},
	unique-id = {34473960},
	issn = {0300-3604},
	abstract = {Haematococcus lacustris is an important species of green algae because it produces the high-value carotenoid astaxanthin. Astaxanthin production is enhanced by various stress conditions causing the transformation of green vegetative cells to red cells with high amounts of astaxanthin, which plays various photoprotective and antioxidant roles. Although intensive research has been conducted to reveal the regulation of astaxanthin production, the photosynthetic capacity of the various cell forms is unresolved at the single-cell level. In this work, we characterized the photosynthetic and morphological changes of Haematococcus cells, using a combination of microfluidic tools and microscopic chlorophyll fluorescence imaging. We found marked but reversible changes in the variable chlorophyll fluorescence signatures upon the transformation of green cells to red cells, and we propose that the photosynthetic activity as revealed by single-cell chlorophyll fluorescence kinetics serves as a useful phenotypic marker of the different cell forms of Haematococcus.},
	keywords = {PHOTOSYNTHESIS; CHLOROPHYLL FLUORESCENCE; astaxanthin; photoprotection; cyclic electron flow; Haematococcus lacustris; GREEN-ALGA; VIVO CHLOROPHYLL FLUORESCENCE; LOW-WAVE PHENOMENON; PLUVIALIS},
	year = {2023},
	eissn = {1573-9058},
	pages = {473-482}
}

@article{MTMT:34043896,
	title = {Characterization of the Flash-Induced Fluorescence Wave Phenomenon in the Coral Endosymbiont Algae, Symbiodiniaceae},
	url = {https://m2.mtmt.hu/api/publication/34043896},
	author = {Mohammad Aslam, Sabit and Vass, Imre and Szabó, Milán},
	doi = {10.3390/ijms24108712},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {24},
	unique-id = {34043896},
	issn = {1661-6596},
	abstract = {The dinoflagellate algae, Symbiodiniaceae, are significant symbiotic partners of corals due to their photosynthetic capacity. The photosynthetic processes of the microalgae consist of linear electron transport, which provides the energetic balance of ATP and NADPH production for CO2 fixation, and alternative electron transport pathways, including cyclic electron flow, which ensures the elevated ATP requirements under stress conditions. Flash-induced chlorophyll fluorescence relaxation is a non-invasive tool to assess the various electron transport pathways. A special case of fluorescence relaxation, the so-called wave phenomenon, was found to be associated with the activity of NAD(P)H dehydrogenase (NDH) in microalgae. We showed previously that the wave phenomenon existed in Symbiodiniaceae under acute heat stress and microaerobic conditions, however, the electron transport processes related to the wave phenomenon remained unknown. In this work, using various inhibitors, we show that (i) the linear electron transport has a crucial role in the formation of the wave, (ii) the inhibition of the donor side of Photosystem II did not induce the wave, whereas inhibition of the Calvin-Benson cycle accelerated it, (iii) the wave phenomenon was related to the operation of type II NDH (NDH-2). We therefore propose that the wave phenomenon is an important marker of the regulation of electron transport in Symbiodiniaceae.},
	keywords = {INHIBITION; PHOTOSYSTEM-II; PHOTOSYNTHESIS; CHLOROPHYLL FLUORESCENCE; CHLOROPHYLL FLUORESCENCE; Biochemistry & Molecular Biology; symbiotic microalgae; linear electron flow},
	year = {2023},
	eissn = {1422-0067}
}

@article{MTMT:33608945,
	title = {The slow-phase of chlorophyll fluorescence induction curve reflects the electron transport rates of Photosystem II in vivo in Chlorella vulgaris},
	url = {https://m2.mtmt.hu/api/publication/33608945},
	author = {Bates, Harvey and Zavafer, Alonso and Szabó, Milán and Ralph, Peter J.},
	doi = {10.1007/s10811-022-02890-0},
	journal-iso = {J APPL PHYCOL},
	journal = {JOURNAL OF APPLIED PHYCOLOGY},
	volume = {35},
	unique-id = {33608945},
	issn = {0921-8971},
	abstract = {Ideally methods and parameters to assess photosynthesis in the microalga Chlorella vulgaris must be rapid, non-invasive, accurate, and simple to implement. Methods such as the Quantum Yield of photosynthesis (phi(Po) or F-v/F-m) yield rapid results but do not reflect the actual electron turnover of PSII (ETRII). Alternatively, methods that calculate ETRII take several minutes to execute, require expensive instrumentation, and manual input. In this work, we describe a method to estimate ETRII in real-time via predictive statistic modelling. This method utilises the Kautsky induction curve and a low-cost chlorophyll a fluorometer. Our model optimises the fit of linear regressions between the normalized fluorescence intensity after F-m (named S-ETR) and ETRII measurements using traditional methods. This allows for an estimation of ETRII in real-time through Kautsky induction curves alone. S-ETR can then be used as a faster alternative to quenching analysis to determine bioenergetics performance during steady state and can be assessed with any chlorophyll fluorometer capable of measuring the Kautsky induction curve. The S-ETR parameter allows for a high-throughput and high-resolution assessment of photosynthesis while being non-invasive, having lower production costs, and with lower technical requirements. Furthermore, the biophysical bases of the method are discussed.},
	keywords = {BIOMASS; Electron Transport; Photosystem II; Biotechnology & Applied Microbiology},
	year = {2023},
	eissn = {1573-5176},
	pages = {109-116}
}

@article{MTMT:33025641,
	title = {Heat-Induced Photosynthetic Responses of Symbiodiniaceae Revealed by Flash-Induced Fluorescence Relaxation Kinetics},
	url = {https://m2.mtmt.hu/api/publication/33025641},
	author = {Mohammad Aslam, Sabit and Patil, Priyanka Pradeep and Vass, Imre and Szabó, Milán},
	doi = {10.3389/fmars.2022.932355},
	journal-iso = {FRONT MAR SCI},
	journal = {FRONTIERS IN MARINE SCIENCE},
	volume = {9},
	unique-id = {33025641},
	year = {2022},
	eissn = {2296-7745}
}

@article{MTMT:32832922,
	title = {Viable protoplast formation of the coral endosymbiont alga Symbiodinium spp. in a microfluidics platform},
	url = {https://m2.mtmt.hu/api/publication/32832922},
	author = {Bashir, Faiza and Kovács, Sándor and Ábrahám, Ágnes and Nagy, Krisztina and Ayaydin, Ferhan and Kelemen-Valkony, Ildikó and Ferenc, Györgyi and Galajda, Péter and Tóth, Szilvia Zita and Sass, László and Kós, Péter and Vass, Imre and Szabó, Milán},
	doi = {10.1039/D2LC00130F},
	journal-iso = {LAB CHIP},
	journal = {LAB ON A CHIP},
	volume = {22},
	unique-id = {32832922},
	issn = {1473-0197},
	abstract = {Symbiodiniaceae is an important dinoflagellate family which lives in endosymbiosis with reef invertebrates, including coral polyps, making them central to the holobiont. With coral reefs currently under extreme threat from climate change, there is a pressing need to improve our understanding on the stress tolerance and stress avoidance mechanisms of Symbiodinium spp. Reactive oxygen species (ROS) such as singlet oxygen are central players in mediating various stress responses; however, the detection of ROS using specific dyes is still far from definitive in intact Symbiodinium cells due to the hindrance of uptake of certain fluorescent dyes because of the presence of the cell wall. Protoplast technology provides a promising platform for studying oxidative stress with the main advantage of removed cell wall, however the preparation of viable protoplasts remains a significant challenge. Previous studies have successfully applied cellulose-based protoplast preparation in Symbiodiniaceae; however, the protoplast formation and regeneration process was found to be suboptimal. Here, we present a microfluidics-based platform which allowed protoplast isolation from individually trapped Symbiodinium cells, by using a precisely adjusted flow of cell wall digestion enzymes (cellulase and macerozyme). Trapped single cells exhibited characteristic changes in their morphology, cessation of cell division and a slight decrease in photosynthetic activity during protoplast formation. Following digestion and transfer to regeneration medium, protoplasts remained photosynthetically active, regrew cell walls, regained motility, and entered exponential growth. Elevated flow rates in the microfluidic chambers resulted in somewhat faster protoplast formation; however, cell wall digestion at higher flow rates partially compromised photosynthetic activity. Physiologically competent protoplasts prepared from trapped cells in microfluidic chambers allowed for the first time the visualization of the intracellular localization of singlet oxygen (using Singlet Oxygen Sensor Green dye) in Symbiodiniaceae, potentially opening new avenues for studying oxidative stress.},
	year = {2022},
	eissn = {1473-0189},
	pages = {2986-2999},
	orcid-numbers = {Ferenc, Györgyi/0000-0002-3456-319X}
}

@article{MTMT:32799849,
	title = {Characterization of the Wave Phenomenon in Flash-Induced Fluorescence Relaxation and Its Application to Study Cyclic Electron Pathways in Microalgae},
	url = {https://m2.mtmt.hu/api/publication/32799849},
	author = {Patil, Priyanka Pradeep and Vass, Imre and Szabó, Milán},
	doi = {10.3390/ijms23094927},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {23},
	unique-id = {32799849},
	issn = {1661-6596},
	year = {2022},
	eissn = {1422-0067},
	orcid-numbers = {Patil, Priyanka Pradeep/0000-0003-2079-6248}
}

@article{MTMT:32677799,
	title = {Characterization of the wave phenomenon of flash-induced chlorophyll fluorescence in Chlamydomonas reinhardtii},
	url = {https://m2.mtmt.hu/api/publication/32677799},
	author = {Patil, Priyanka Pradeep and Mohammad Aslam, Sabit and Vass, Imre and Szabó, Milán},
	doi = {10.1007/s11120-022-00900-3},
	journal-iso = {PHOTOSYNTH RES},
	journal = {PHOTOSYNTHESIS RESEARCH},
	volume = {152},
	unique-id = {32677799},
	issn = {0166-8595},
	year = {2022},
	eissn = {1573-5079},
	pages = {235-244}
}

@article{MTMT:32473846,
	title = {Investigating the impact of light quality on macromolecular of Chaetoceros muelleri},
	url = {https://m2.mtmt.hu/api/publication/32473846},
	author = {Iwasaki, Kenji and Szabó, Milán and Tamburic, Bojan and Evenhuis, Christian and Zavafer, Alonso and Kuzhiumparambil, Unnikrishnan and Ralph, Peter},
	doi = {10.1071/FP21131},
	journal-iso = {FUNCT PLANT BIOL},
	journal = {FUNCTIONAL PLANT BIOLOGY},
	volume = {49},
	unique-id = {32473846},
	issn = {1445-4408},
	abstract = {Diatoms (Bacillariophyceae) are important to primary productivity of aquatic ecosystems. This algal group is also a valuable source of high value compounds that are utilised as aquaculture feed. The productivity of diatoms is strongly driven by light and CO2 availability, and macro- and micronutrient concentrations. The light dependency of biomass productivity and metabolite composition is well researched in diatoms, but information on the impact of light quality, particularly the productivity return on energy invested when using different monochromatic light sources, remains scarce. In this work, the productivity return on energy invested of improving growth rate, photosynthetic activity, and metabolite productivity of the diatom Chaetoceros muelleri under defined wavelengths (blue, red, and green) as well as while light is analysed. By adjusting the different light qualities to equal photosynthetically utilisable radiation, it was found that the growth rate and photosynthetic oxygen evolution was unchanged under white, blue, and green light, but it was lower under red light. Blue light improved the productivity return on energy invested for biomass, total protein, total lipid, total carbohydrate, and in fatty acids production, which would suggest that blue light should be used for aquaculture feed production.},
	keywords = {Diatoms; ACCLIMATION; GREEN; GROWTH-RATE; diatom; BLUE-LIGHT; MICROALGAE; MICROALGAE; Blue light; PHOTOBIOREACTORS; metabolic profile; Aquatic ecosystems; NANNOCHLOROPSIS-OCULATA; Chaetoceros muelleri; BIOTECHNOLOGICALLY RELEVANT PRODUCTS; EMITTING-DIODES LEDS; DIATOM CULTIVATION},
	year = {2022},
	eissn = {1445-4416},
	pages = {554-564},
	orcid-numbers = {Tamburic, Bojan/0000-0001-5720-9380; Zavafer, Alonso/0000-0002-8905-1618}
}

@article{MTMT:32155102,
	title = {Photoinhibition, photo-ecophysiology, and biophysics, a special issue in honor of Wah Soon Chow PREFACE},
	url = {https://m2.mtmt.hu/api/publication/32155102},
	author = {Szabó, Milán and Zavafer, Alonso},
	doi = {10.1007/s11120-021-00865-9},
	journal-iso = {PHOTOSYNTH RES},
	journal = {PHOTOSYNTHESIS RESEARCH},
	volume = {149},
	unique-id = {32155102},
	issn = {0166-8595},
	keywords = {PARAMETERS; OXYGEN},
	year = {2021},
	eissn = {1573-5079},
	pages = {1-3}
}