TY - JOUR AU - Dudits, Dénes AU - Cseri, András AU - Török, Katalin AU - Vankova, Radomira AU - Dobrev, Petre I. AU - Sass, László AU - Steinbach, Gábor AU - Kelemen-Valkony, Ildikó AU - Zombori, Zoltán AU - Ferenc, Györgyi AU - Ayaydin, Ferhan TI - Manifestation of Triploid Heterosis in the Root System after Crossing Diploid and Autotetraploid Energy Willow Plants JF - GENES J2 - GENES-BASEL VL - 14 PY - 2023 IS - 10 PG - 17 SN - 2073-4425 DO - 10.3390/genes14101929 UR - https://m2.mtmt.hu/api/publication/34201709 ID - 34201709 N1 - Funding Agency and Grant Number: Hungarian government [GINOP-2.2.1-15-2017-00081, 2020-1.1.2-PIACI-KFI-2020-00117]; European Union [739593] Funding text: This work was funded by grant no. GINOP-2.2.1-15-2017-00081 and 2020-1.1.2-PIACI-KFI-2020-00117 from the Hungarian government. F.A. received support from the European Union's Horizon 2020 research and innovation program with grant number 739593. AB - Successful use of woody species in reducing climatic and environmental risks of energy shortage and spreading pollution requires deeper understanding of the physiological functions controlling biomass productivity and phytoremediation efficiency. Targets in the breeding of energy willow include the size and the functionality of the root system. For the combination of polyploidy and heterosis, we have generated triploid hybrids (THs) of energy willow by crossing autotetraploid willow plants with leading cultivars (Tordis and Inger). These novel Salix genotypes (TH3/12, TH17/17, TH21/2) have provided a unique experimental material for characterization of Mid-Parent Heterosis (MPH) in various root traits. Using a root phenotyping platform, we detected heterosis (TH3/12: MPH 43.99%; TH21/2: MPH 26.93%) in the size of the root system in soil. Triploid heterosis was also recorded in the fresh root weights, but it was less pronounced (MPH%: 9.63–19.31). In agreement with root growth characteristics in soil, the TH3/12 hybrids showed considerable heterosis (MPH: 70.08%) under in vitro conditions. Confocal microscopy-based imaging and quantitative analysis of root parenchyma cells at the division–elongation transition zone showed increased average cell diameter as a sign of cellular heterosis in plants from TH17/17 and TH21/2 triploid lines. Analysis of the hormonal background revealed that the auxin level was seven times higher than the total cytokinin contents in root tips of parental Tordis plants. In triploid hybrids, the auxin–cytokinin ratios were considerably reduced in TH3/12 and TH17/17 roots. In particular, the contents of cytokinin precursor, such as isopentenyl adenosine monophosphate, were elevated in all three triploid hybrids. Heterosis was also recorded in the amounts of active gibberellin precursor, GA19, in roots of TH3/12 plants. The presented experimental findings highlight the physiological basics of triploid heterosis in energy willow roots. LA - English DB - MTMT ER - TY - JOUR AU - Misra, Ramprasad AU - Das, Ishita AU - Dér, András AU - Steinbach, Gábor AU - Shim, Jin-gon AU - Busse, Wayne AU - Jung, Kwang-Hwan AU - Zimányi, László AU - Sheves, Mordechai TI - Impact of protein–chromophore interaction on the retinal excited state and photocycle of Gloeobacter rhodopsin: role of conserved tryptophan residues JF - CHEMICAL SCIENCE J2 - CHEM SCI VL - 14 PY - 2023 SP - 9951 EP - 9958 PG - 8 SN - 2041-6520 DO - 10.1039/D3SC02961A UR - https://m2.mtmt.hu/api/publication/34129738 ID - 34129738 N1 - Funding Agency and Grant Number: Kimmelman Center for Biomolecular Structure and Assembly, Israel; National Research and Development Office, Hungary [NKFI-1 K-124922, 2018-1.2.1-NKP-2018-00009]; Ministry for National Economy of Hungary [GINOP-2.3.2-15-2016-00001]; Katzir-Makineni Chair in Chemistry Funding text: The work was.nancially supported by the Kimmelman Center for Biomolecular Structure and Assembly, Israel, the National Research and Development Office, Hungary (NKFI-1 K-124922 and 2018-1.2.1-NKP-2018-00009), and the Ministry for National Economy of Hungary (GINOP-2.3.2-15-2016-00001). RM thanks Prof. P. Hegemann for constant encouragements. M. S. holds the Katzir-Makineni Chair in Chemistry. AB - The conserved tryptophan residues in the vicinity of the retinal in microbial rhodopsins affect the retinal excited state charge distribution, increase the isomerization quantum yield, and affect the retinal double bond reisomerization rate during photocycle. LA - English DB - MTMT ER - TY - JOUR AU - Masuda, Takako AU - Inomura, Keisuke AU - Gao, Meng AU - Armin, Gabrielle AU - Kotabová, Eva AU - Bernát, Gábor AU - Lawrenz-Kendrick, Evelyn AU - Lukeš, Martin AU - Bečková, Martina AU - Steinbach, Gábor AU - Komenda, Josef AU - Prášil, Ondřej TI - The balance between photosynthesis and respiration explains the niche differentiation between Crocosphaera and Cyanothece JF - COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL J2 - CSBJ VL - 21 PY - 2023 SP - 58 EP - 65 PG - 8 SN - 2001-0370 DO - 10.1016/j.csbj.2022.11.029 UR - https://m2.mtmt.hu/api/publication/33265258 ID - 33265258 N1 - Funding Agency and Grant Number: Czech Research Foundation [20-17627S, 19-29225X]; JSPS KAKENHI [20H03059, 22H05201]; U.S. National Science Foundation [OCE-2048373, SUB0000525, OIA-1655221]; Rhode Island Science and Technology Advisory Council [AWD10732]; National Research, Development and Innovation Office of Hungary, NKFIH [K 140351, RRF-2.3.1-21-2021] Funding text: This research was financially supported by Czech Research Foundation GACCR (project 20-17627S to OP and TM and project 19-29225X to MB and JK) , JSPS KAKENHI (Grant Nos. 20H03059, 22H05201 to TM) , the U.S. National Science Foundation under EPS-CoR Cooperative Agreement (OIA-1655221 to KI) , the U.S. National Science Foundation (OCE-2048373, subaward SUB0000525 from Princeton University to KI) and the Rhode Island Science and Technology Advisory Council (AWD10732 to KI) , National Research, Development and Innovation Office of Hungary, NKFIH (awards K 140351 and RRF-2.3.1-21-2021 to GB) . LA - English DB - MTMT ER - TY - JOUR AU - Pleckaitis, Marijus AU - Habach, Fayez AU - Kontenis, Lukas AU - Steinbach, Gábor AU - Jarockyte, Greta AU - Kalnaityte, Agne AU - Racskóné Domonkos, Ildikó AU - Akhtar, Parveen AU - Alizadeh, Mehdi AU - Bagdonas, Saulius AU - Karabanovas, Vitalijus AU - Garab, Győző AU - Rotomskis, Ricardas AU - Barzda, Virginijus TI - Structure and principles of self-assembly of giant "sea urchin" type sulfonatophenyl porphine aggregates JF - NANO RESEARCH J2 - NANO RES VL - 15 PY - 2022 IS - 6 SP - 5527 EP - 5537 PG - 11 SN - 1998-0124 DO - 10.1007/s12274-021-4048-x UR - https://m2.mtmt.hu/api/publication/32763244 ID - 32763244 N1 - Funding Agency and Grant Number: European Regional Development FundEuropean Commission [01.2.2.-LMT-K-718-02-0016]; Research Council of Lithuania (LMTLT)Research Council of Lithuania (LMTLT); Natural Sciences and Engineering Research Council of Canada (NSERC)Natural Sciences and Engineering Research Council of Canada (NSERC) [RGPIN-2017-06923, DGDND-2017-00099]; Hungarian Ministry for National Economy [GINOP-2.3.2-15-2016-00058, GINOP-2.3.2-15-2016-00001, ELKH KU-37/2020]; Eotvos Lorand Research Network Funding text: This work was supported by European Regional Development Fund (No. 01.2.2.-LMT-K-718-02-0016) under grant agreement with the Research Council of Lithuania (LMTLT). Support was also provided by the Natural Sciences and Engineering Research Council of Canada (NSERC) (Nos. RGPIN-2017-06923 and DGDND-2017-00099). I. D. was supported by GINOP-2.3.2-15-2016-00058 and G. S. by GINOP-2.3.2-15-2016-00001 and ELKH KU-37/2020 grants from the Hungarian Ministry for National Economy and the Eotvos Lorand Research Network, respectively. AB - Principles of molecular self-assembly into giant hierarchical structures of hundreds of micrometers in size are studied in aggregates of meso-tetra(4-sulfonatophenyl)porphine (TPPS4). The aggregates form a central tubular core, which is covered with radially protruding filamentous non-branching aggregates. The filaments cluster and orient at varying angles from the core surface and some filaments form bundles. Due to shape resemblance, the structures are termed giant sea urchin (GSU) aggregates. Spectrally resolved fluorescence microscopy reveals J- and H-bands of TPPS4 aggregates in both the central core and the filaments. The fluorescence of the core is quenched while filaments exhibit strong fluorescence. Upon drying, the filament fluorescence gets quenched while the core is less affected, showing stronger relative fluorescence. Fluorescence-detected linear dichroism (FDLD) microscopy reveals that absorption dipoles corresponding to J-bands are oriented along the filament axis. The comparison of FDLD with scanning electron microscopy (SEM) reveals the structure of central core comprised of multilayer ribbons, which wind around the core axis forming a tube. Polarimetric second-harmonic generation (SHG) and third-harmonic generation microscopy exhibits strong signal from the filaments with nonlinear dipoles oriented close to the filament axis, while central core displays very low SHG due to close to centrosymmetric organization. Large chiral nonlinear susceptibility points to helical arrangement of the filaments. The investigation shows that TPPS4 molecules form distinct aggregate types, including chiral nanotubes and nanogranular aggregates that associate into the hierarchical GSU structure, prototypical to complex biological structures. The chiral TPPS4 aggregates can serve as harmonophores for nonlinear microscopy. LA - English DB - MTMT ER - TY - JOUR AU - Delawska, Katerina AU - Divoka, Petra AU - Sedlak, David AU - Kuzma, Marek AU - Saurav, Kumar AU - Macho, Marketa AU - Steinbach, Gábor AU - Hrouzek, Pavel TI - New Insights into Tolytoxin Effect in Human Cancer Cells: Apoptosis Induction and the Relevance of Hydroxyl Substitution of Its Macrolide Cycle on Compound Potency JF - CHEMBIOCHEM J2 - CHEMBIOCHEM VL - 23 PY - 2022 IS - 1 PG - 6 SN - 1439-4227 DO - 10.1002/cbic.202100489 UR - https://m2.mtmt.hu/api/publication/32557476 ID - 32557476 N1 - Funding Agency and Grant Number: Czech Grant AgencyGrant Agency of the Czech Republic [21-05649K]; Ministry of Education, Youth and Sports of the Czech RepublicMinistry of Education, Youth & Sports - Czech Republic [LO1416, RVO: 68378050]; National infrastructure for chemical biology [LM2018130]; Cross-Border cooperation Czech-Bavaria [41, GINOP-2.3.2-15-2016-00001] Funding text: This work was financially supported by the Czech Grant Agency (Discovery of promising chemotherapeutic candidates in cyanobacteria using high-throughput screening: mode of action and molecular targets. - project No. 21-05649K) and further by the Ministry of Education, Youth and Sports of the Czech Republic (project Algatech Plus LO1416 and RVO: 68378050-KAV-NPUI at CZ-OS), National infrastructure for chemical biology LM2018130, Cross-Border cooperation Czech-Bavaria project no. 41 and GINOP-2.3.2-15-2016-00001 grant. We want to thank Jindriska Paichlova, Veronika Broova, Olga Martinkova, Toma Langhammer and Martin Popr for technical assistance. Prof. Andreas Villunger, Ph.D. (Medical University Innsbruck) is acknowledged for providing cell lines HeLa WT and Bax/Bak DKO. AB - Scytophycins, including tolytoxin, represent a class of actin disrupting macrolides with strong antiproliferative effects on human cells. Despite intense research, little attention has been paid to scytophycin-induced cell death or the structural features affecting its potency. We show that tolytoxin and its natural analogue, 7-O-methylscytophycin B, lacking the hydroxyl substitution in its macrolactone ring, differ substantially in their cytotoxic effect. Both compounds increase the level of caspases 3/7, which are the main executioner proteases during apoptosis, in HeLa wild-type (WT) cells. However, no caspase activity was detected in HeLa cells lacking Bax/Bak proteins crucial for caspase activation via the mitochondrial pathway. Obtained data strongly suggests that scytophycins are capable of inducing mitochondria-dependent apoptosis. These findings encourage further research in structure-activity relationships in scytophycins and highlight the potential of these compounds in targeted drug delivery. LA - English DB - MTMT ER - TY - JOUR AU - Radosavljević, Jasna Simonović AU - Mitrović, Aleksandra Lj. AU - Radotić, Ksenija AU - Zimányi, László AU - Garab, Győző AU - Steinbach, Gábor TI - Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 22 PY - 2021 IS - 14 SN - 1661-6596 DO - 10.3390/ijms22147661 UR - https://m2.mtmt.hu/api/publication/32106260 ID - 32106260 N1 - Funding Agency and Grant Number: Hungarian Ministry for National Economy (Economic Development and Innovation Operational Programme) [GINOP-2.3.3-15-201600003, GINOP-2.3.3-15-2016-00030, GINOP-2.1.7-15-2016-00713]; Ministry of Education, Science and Technological Development of the Republic of SerbiaMinistry of Education, Science & Technological Development, Serbia [451-03-9/202114/200053]; National Research Development and Innovation Office of HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [OTKA K 128679]; [GINOP-2.3.2-15-2016-00001] Funding text: The development work was supported by grants from the Hungarian Ministry for National Economy (Economic Development and Innovation Operational Programme, GINOP-2.3.3-15-201600003, GINOP-2.3.3-15-2016-00030, and GINOP-2.1.7-15-2016-00713 projects). Work of G.S. has been funded by GINOP-2.3.2-15-2016-00001 grant. We acknowledge the grant from the Ministry of Education, Science and Technological Development of the Republic of Serbia No 451-03-9/202114/200053. Partial support was obtained from the National Research Development and Innovation Office of Hungary (OTKA K 128679 to G.G.). LA - English DB - MTMT ER - TY - JOUR AU - Cséplő, Ágnes AU - Zsigmond, Laura AU - Andrási, Norbert AU - Abu Baba, Imran AU - Labhane, Nitin M. AU - Pető, Andrea AU - Kolbert, Zsuzsanna AU - Kovács, Hajnalka Éva AU - Steinbach, Gábor AU - Szabados, László AU - Fehér, Attila AU - Rigó, Gábor TI - The AtCRK5 Protein Kinase Is Required to Maintain the ROS NO Balance Affecting the PIN2-Mediated Root Gravitropic Response in Arabidopsis JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 22 PY - 2021 IS - 11 PG - 23 SN - 1661-6596 DO - 10.3390/ijms22115979 UR - https://m2.mtmt.hu/api/publication/32059092 ID - 32059092 N1 - Biological Research Centre (BRC), Institute of Plant Biology, Eötvös Loránd Research Network (ELKH), Szeged, H-6726, Hungary Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, SE-901 83, Sweden Department of Botany, Bhavan’s College Andheri West, Mumbai, 400058, India Department of Plant Biology, University of Szeged, 52. Középfasor, Szeged, H-6726, Hungary Food Chain Safety Center Nonprofit Ltd., Budapest, H-1024, Hungary Kossuth Lajos Sugárút, 72/D, Budapest, Szeged, H-6724, Hungary Cellular Imaging Laboratory, Biological Research Centre, Eötvös Loránd Research Network, Szeged, H-6726, Hungary Export Date: 31 August 2021 Correspondence Address: Rigó, G.; Biological Research Centre (BRC), Hungary; email: rigo.gabor@brc.hu Biological Research Centre (BRC), Institute of Plant Biology, Eötvös Loránd Research Network (ELKH), Szeged, H-6726, Hungary Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, SE-901 83, Sweden Department of Botany, Bhavan’s College Andheri West, Mumbai, 400058, India Department of Plant Biology, University of Szeged, 52. Középfasor, Szeged, H-6726, Hungary Food Chain Safety Center Nonprofit Ltd., Budapest, H-1024, Hungary Kossuth Lajos Sugárút, 72/D, Budapest, Szeged, H-6724, Hungary Cellular Imaging Laboratory, Biological Research Centre, Eötvös Loránd Research Network, Szeged, H-6726, Hungary Export Date: 1 September 2021 Correspondence Address: Rigó, G.; Biological Research Centre (BRC), Hungary; email: rigo.gabor@brc.hu Biological Research Centre (BRC), Institute of Plant Biology, Eötvös Loránd Research Network (ELKH), Szeged, H-6726, Hungary Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, SE-901 83, Sweden Department of Botany, Bhavan’s College Andheri West, Mumbai, 400058, India Department of Plant Biology, University of Szeged, 52. Középfasor, Szeged, H-6726, Hungary Food Chain Safety Center Nonprofit Ltd., Budapest, H-1024, Hungary Kossuth Lajos Sugárút, 72/D, Budapest, Szeged, H-6724, Hungary Cellular Imaging Laboratory, Biological Research Centre, Eötvös Loránd Research Network, Szeged, H-6726, Hungary Export Date: 16 September 2021 Correspondence Address: Rigó, G.; Biological Research Centre (BRC), Hungary; email: rigo.gabor@brc.hu LA - English DB - MTMT ER - TY - JOUR AU - Bernát, Gábor AU - Zavřel, Tomáš AU - Kotabová, Eva AU - Kovács, László AU - Steinbach, Gábor AU - Vörös, Lajos AU - Prášil, Ondřej AU - Somogyi, Boglárka AU - Tóth, Viktor R. TI - Photomorphogenesis in the Picocyanobacterium Cyanobium gracile Includes Increased Phycobilisome Abundance Under Blue Light, Phycobilisome Decoupling Under Near Far-Red Light, and Wavelength-Specific Photoprotective Strategies JF - FRONTIERS IN PLANT SCIENCE J2 - FRONT PLANT SCI VL - 12 PY - 2021 PG - 16 SN - 1664-462X DO - 10.3389/fpls.2021.612302 UR - https://m2.mtmt.hu/api/publication/31920580 ID - 31920580 LA - English DB - MTMT ER - TY - JOUR AU - Kaňa, Radek AU - Steinbach, Gábor AU - Sobotka, Roman AU - Vámosi, György AU - Komenda, Josef TI - Fast Diffusion of the Unassembled PetC1-GFP Protein in the Cyanobacterial Thylakoid Membrane JF - LIFE-BASEL J2 - LIFE-BASEL VL - 11 PY - 2021 IS - 1 SN - 2075-1729 DO - 10.3390/life11010015 UR - https://m2.mtmt.hu/api/publication/31808899 ID - 31808899 N1 - Center ALGATECH, Institute of Microbiology, Academy of Sciences of the Czech Republic, Třeboň, 37901, Czech Republic Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, Szeged, 6727, Hungary Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary Export Date: 15 January 2021 Correspondence Address: Kaňa, R.; Center ALGATECH, Institute of Microbiology, Academy of Sciences of the Czech RepublicCzech Republic; email: kana@alga.cz Center ALGATECH, Institute of Microbiology, Academy of Sciences of the Czech Republic, Třeboň, 37901, Czech Republic Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, Szeged, 6727, Hungary Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary Cited By :1 Export Date: 17 May 2021 Correspondence Address: Kaňa, R.; Center ALGATECH, Czech Republic; email: kana@alga.cz LA - English DB - MTMT ER - TY - JOUR AU - Ünnep, Renáta AU - Paul, S. AU - Zsíros, Ottó AU - Kovács, László AU - Székely, Noémi Kinga AU - Steinbach, Gábor AU - Appavou, M.-S. AU - Porcar, L. AU - Holzwarth, A.R. AU - Garab, Győző AU - Nagy, Gergely TI - Thylakoid membrane reorganizations revealed by small-angle neutron scattering of Monstera deliciosa leaves associated with non-photochemical quenching JF - OPEN BIOLOGY J2 - OPEN BIOL VL - 10 PY - 2020 IS - 9 PG - 12 SN - 2046-2441 DO - 10.1098/rsob.200144 UR - https://m2.mtmt.hu/api/publication/31614001 ID - 31614001 AB - Non-photochemical quenching (NPQ) is an important photoprotective mechanism in plants and algae. Although the process is extensively studied, little is known about its relationship with ultrastructural changes of the thylakoid membranes. In order to better understand this relationship, we studied the effects of illumination on the organization of thylakoid membranes in Monstera deliciosa leaves. This evergreen species is known to exhibit very large NPQ and to possess giant grana with dozens of stacked thylakoids. It is thus ideally suited for small-angle neutron scattering measurements (SANS) - a non-invasive technique, which is capable of providing spatially and statistically averaged information on the periodicity of the thylakoid membranes and their rapid reorganizations in vivo. We show that NPQ-inducing illumination causes a strong decrease in the periodic order of granum thylakoid membranes. Development of NPQ and light-induced ultrastructural changes, as well as the relaxation processes, follow similar kinetic patterns. Surprisingly, whereas NPQ is suppressed by diuron, it impedes only the relaxation of the structural changes and not its formation, suggesting that structural changes do not cause but enable NPQ. We also demonstrate that the diminishment of SANS peak does not originate from light-induced redistribution and reorientation of chloroplasts inside the cells. © 2020 The Authors. LA - English DB - MTMT ER -