TY - JOUR AU - Varga, Gergely István AU - Csordás, Gábor AU - Jankovics, Ferenc AU - Lukacsovich, Tamás AU - Cinege, Gyöngyi Ilona AU - Sinka, Rita AU - Kurucz, Judit Éva AU - Andó, István AU - Honti, Viktor TI - Headcase, a novel regulator of hemocyte differentiation in Drosophila Melanogaster JF - IMMUNOLÓGIAI SZEMLE J2 - IMMUNOLÓGIAI SZEMLE VL - 10 PY - 2018 IS - 3 SP - 51 EP - 51 PG - 1 SN - 2061-0203 UR - https://m2.mtmt.hu/api/publication/30362934 ID - 30362934 LA - English DB - MTMT ER - TY - BOOK AU - Gyovai, Márk AU - Kálmán, Renáta AU - Kapa, Mátyás AU - Lukacsovich, Tamás AU - Martonovics, Bernadett AU - Pákozdi, Zita AU - Petrik, Béla AU - Schadl, György AU - Schadl-Baranyai, Helga AU - Udvary, Sándor ED - Szabó, Imre TI - Szakvizsga felkészítő tankönyv a bírósági végrehajtói szakvizsgához ET - 0 PB - Magyar Közlöny Lap- és Könyvkiadó CY - Budapest PY - 2018 SN - 9786155710551 UR - https://m2.mtmt.hu/api/publication/3423867 ID - 3423867 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Bence, Melinda AU - Jankovics, Ferenc AU - Lukacsovich, Tamás AU - Erdélyi, Miklós TI - Combining the auxin-inducible degradation system with CRISPR/Cas9-based genome editing for the conditional depletion of endogenous Drosophila melanogaster proteins JF - FEBS JOURNAL J2 - FEBS J VL - 284 PY - 2017 IS - 7 SP - 1056 EP - 1069 PG - 14 SN - 1742-464X DO - 10.1111/febs.14042 UR - https://m2.mtmt.hu/api/publication/3231445 ID - 3231445 AB - Inducible protein degradation techniques have considerable advantages over classical genetic approaches, which generate loss-of-function phenotypes at the gene or mRNA level. The plant-derived auxin-inducible degradation system (AID) is a promising technique which enables the degradation of target proteins tagged with the AID motif in nonplant cells. Here, we present a detailed characterization of this method employed during the adult oogenesis of Drosophila. Furthermore, with the help of CRISPR/Cas9-based genome editing, we improve the utility of the AID system in the conditional elimination of endogenously expressed proteins. We demonstrate that the AID system induces efficient and reversible protein depletion of maternally provided proteins both in the ovary and the early embryo. Moreover, the AID system provides a fine spatiotemporal control of protein degradation and allows for the generation of different levels of protein knockdown in a well-regulated manner. These features of the AID system enable the unraveling of the discrete phenotypes of genes with highly complex functions. We utilized this system to generate a conditional loss-of-function allele which allows for the specific degradation of the Vasa protein without affecting its alternative splice variant (solo) and the vasa intronic gene (vig). With the help of this special allele, we demonstrate that dramatic decrease of Vasa protein in the vitellarium does not influence the completion of oogenesis as well as the establishment of proper anteroposterior and dorsoventral polarity in the developing oocyte. Our study suggests that both the localization and the translation of gurken mRNA in the vitellarium is independent from Vasa. LA - English DB - MTMT ER - TY - JOUR AU - Márkus, Róbert AU - Lerner, Zita AU - Honti, Viktor AU - Csordás, Gábor AU - Zsámboki, János AU - Cinege, Gyöngyi Ilona AU - Párducz, Árpád AU - Lukacsovich, Tamás AU - Kurucz, Judit Éva AU - Andó, István TI - Multinucleated Giant Hemocytes Are Effector Cells in Cell-Mediated Immune Responses of Drosophila JF - JOURNAL OF INNATE IMMUNITY J2 - J INNATE IMMUN VL - 7 PY - 2015 IS - 4 SP - 340 EP - 353 PG - 14 SN - 1662-811X DO - 10.1159/000369618 UR - https://m2.mtmt.hu/api/publication/2853634 ID - 2853634 N1 - Megjegyzés-25258522 Hiányzó Jelleg: 'JOUR\n\nArticle' Admin megjegyzés-25258522 tblcategory: (Category) ('JOUR\n\nArticle') #Jelleg AB - We identified and characterized a so far unrecognized cell type, dubbed the multinucleated giant hemocyte (MGH), in the ananassae subgroup of Drosophilidae. Here, we describe the functional and ultrastructural characteristics of this novel blood cell type as well as its characterization with a set of discriminative immunological markers. MGHs are encapsulating cells that isolate and kill the parasite without melanization. They share some properties with but differ considerably from lamellocytes, the encapsulating cells of Drosophila melanogaster, the broadly used model organism in studies of innate immunity. MGHs are nonproliferative effector cells that are derived from phagocytic cells of the sessile tissue and the circulation, but do not exhibit phagocytic activity. In contrast to lamellocytes, MGHs are gigantic cells with filamentous projections and contain many nuclei, which are the result of the fusion of several cells. Although the structure of lamellocytes and MGHs differ remarkably, their function in the elimination of parasites is similar, which is potentially the result of the convergent evolution of interactions between hosts and parasites in different geographic regions. MGHs are highly motile and share several features with mammalian multinucleated giant cells, a syncytium of macrophages formed during granulomatous inflammation. © 2015 S. Karger AG, Basel LA - English DB - MTMT ER - TY - CHAP AU - Varga, Gergely István AU - Honti, Viktor AU - Csordás, Gábor AU - Jankovics, Ferenc AU - Márkus, Róbert AU - Lukacsovich, Tamás AU - Kurucz, Judit Éva AU - Andó, István ED - Vágvölgyi, Csaba ED - Szekeres, András TI - Vérsejt-differenciálódás vizsgálata a Drosophila melanogaster hematopoietikus kompartmentumaiban T2 - A biológia jövője, a jövő biológusai PB - JATEPress CY - Szeged SN - 9789633152126 PY - 2014 SP - 103 EP - 113 PG - 11 UR - https://m2.mtmt.hu/api/publication/2765598 ID - 2765598 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Vilmos, Péter AU - Bujna, Ágnes AU - Szuperák, Milán AU - Havelda, Zoltán AU - Várallyay, Éva AU - Szabad, János AU - Kucerova, L AU - Somogyi, Kálmán AU - Kristó, Ildikó AU - Lukacsovich, Tamás AU - Jankovics, Ferenc AU - Henn, László AU - Erdélyi, Miklós TI - Viability, Longevity, and Egg Production ofDrosophila melanogasterAre Regulatedby the miR-282 microRNA JF - GENETICS J2 - GENETICS VL - 195 PY - 2013 IS - 2 SP - 469 EP - 480 PG - 12 SN - 0016-6731 DO - 10.1534/genetics.113.153585 UR - https://m2.mtmt.hu/api/publication/2421415 ID - 2421415 AB - The first microRNAs were discovered some 20 years ago, but only a small fraction of the microRNA-encoding genes have been described in detail yet. Here we report the molecular analysis of a computationally predicted Drosophila melanogaster microRNA gene, mir-282. We show that the mir-282 gene is the source of a 4.9 kb long primary transcript with a 5' cap and a 3' polyA sequence and a mature microRNA of about 25 bp. Our data strongly suggest the existence of an independent mir-282 gene conserved in holometabolic insects. We give evidence that the mir-282 locus encodes a functional transcript which influences viability, longevity, and egg production in Drosophila. We identify the nervous system-specific adenylate cyclase (rutabaga) as a target of miR-282 and assume that one of the main functions of mir-282 is the regulation of adenylate cyclase activity in the nervous system during metamorphosis. LA - English DB - MTMT ER - TY - JOUR AU - Vilmos, Péter AU - Jankovics, Ferenc AU - Szathmári, Margit AU - Lukacsovich, Tamás AU - Henn, László AU - Erdélyi, Miklós TI - Live imaging reveals that the Drosophila actin-binding ERM protein, moesin, co-localizes with the mitotic spindle JF - EUROPEAN JOURNAL OF CELL BIOLOGY J2 - EUR J CELL BIOL VL - 88 PY - 2009 IS - 10 SP - 609 EP - 619 PG - 11 SN - 0171-9335 DO - 10.1016/j.ejcb.2009.05.006 UR - https://m2.mtmt.hu/api/publication/1920806 ID - 1920806 N1 - Megjegyzés-22176398 DI: 10.1016/j.ejcb.2009.05.006 LA - English DB - MTMT ER - TY - JOUR AU - Csikós, György AU - Lippai, Mónika AU - Lukacsovich, Tamás AU - Juhász, Gábor AU - Henn, László AU - Erdélyi, Miklós AU - Maróy, Péter AU - Sass, Miklós TI - A novel role for the Drosophila epsin (lqf): involvement in autophagy JF - AUTOPHAGY J2 - AUTOPHAGY VL - 5 PY - 2009 IS - 5 SP - 636 EP - 648 PG - 13 SN - 1554-8627 DO - 10.4161/auto.5.5.8168 UR - https://m2.mtmt.hu/api/publication/1286292 ID - 1286292 N1 - Department of Anatomy; Cell and Developmental Biology, Eötvös Lóránd University, Budapest, Hungary Department of Developmental and Cell Biology, Developmental Biology Center, University of California Irvine, Irvine, CA, United States Institute of Genetics, Biological Research Center, Hungarian Academy of Science, Szeged, Hungary Department of Genetics, University of Szeged, Szeged, Hungary Cited By :11 Export Date: 21 September 2022 Correspondence Address: Sass, M.; Department of Anatomy; Cell and Developmental Biology, , Budapest, Hungary; email: msass@elte.hu LA - English DB - MTMT ER - TY - JOUR AU - Vilmos, Péter AU - Henn, László AU - Szatmári, Anna Mária AU - Lukacsovich, Tamás AU - Sipos, László Attila AU - Erdélyi, Miklós TI - Application of the dual-tagging gene trap method combined with a novel automatic selection system to identify genes involved in germ cell development in Drosophila melanogaster JF - ACTA BIOLOGICA HUNGARICA (1983-2018) J2 - ACTA BIOL HUNG VL - 58 PY - 2007 IS - Suppl.7. SP - 81 EP - 94 PG - 14 SN - 0236-5383 DO - 10.1556/ABiol.58.2007.Suppl.7 UR - https://m2.mtmt.hu/api/publication/1915224 ID - 1915224 N1 - Megjegyzés-22128090 SU: Suppl. S Megjegyzés-22176414 SU: Suppl. S Megjegyzés-22232695 Z9: 1 SU: S DI: 10.1556/ABiol.58.2007.Suppl.7 Megjegyzés-22232748 Z9: 1 SU: S DI: 10.1556/ABiol.58.2007.Suppl.7 LA - English DB - MTMT ER - TY - JOUR AU - Kurucz, Judit Éva AU - Márkus, Róbert AU - Zsámboki, János AU - Medzihradszky F., Katalin AU - Darula, Zsuzsanna AU - Vilmos, Péter AU - Udvardy, Andor AU - Krausz, Ildikó AU - Lukacsovich, Tamás AU - Gateff, E AU - Zettervall, CJ AU - Hultmark, D AU - Andó, István TI - Nimrod, a Putative Phagocytosis Receptor With Egf Repeats in Drosophila Plasmatocytes JF - CURRENT BIOLOGY J2 - CURR BIOL VL - 17 PY - 2007 IS - 7 SP - 649 EP - 654 PG - 6 SN - 0960-9822 DO - 10.1016/j.cub.2007.02.041 UR - https://m2.mtmt.hu/api/publication/1915010 ID - 1915010 N1 - Megjegyzés-22232309 Z9: 60 DI: 10.1016/j.cub.2007.02.041 LA - English DB - MTMT ER -