@article{MTMT:30362934, title = {Headcase, a novel regulator of hemocyte differentiation in Drosophila Melanogaster}, url = {https://m2.mtmt.hu/api/publication/30362934}, author = {Varga, Gergely István and Csordás, Gábor and Jankovics, Ferenc and Lukacsovich, Tamás and Cinege, Gyöngyi Ilona and Sinka, Rita and Kurucz, Judit Éva and Andó, István and Honti, Viktor}, journal-iso = {IMMUNOLÓGIAI SZEMLE}, journal = {IMMUNOLÓGIAI SZEMLE}, volume = {10}, unique-id = {30362934}, issn = {2061-0203}, year = {2018}, pages = {51-51}, orcid-numbers = {Varga, Gergely István/0000-0001-9073-5788; Csordás, Gábor/0000-0001-6871-6839; Sinka, Rita/0000-0003-4040-4184; Andó, István/0000-0002-4648-9396} } @book{MTMT:3423867, title = {Szakvizsga felkészítő tankönyv a bírósági végrehajtói szakvizsgához}, url = {https://m2.mtmt.hu/api/publication/3423867}, isbn = {9786155710551}, author = {Gyovai, Márk and Kálmán, Renáta and Kapa, Mátyás and Lukacsovich, Tamás and Martonovics, Bernadett and Pákozdi, Zita and Petrik, Béla and Schadl, György and Schadl-Baranyai, Helga and Udvary, Sándor}, editor = {Szabó, Imre}, publisher = {Magyar Közlöny Lap- és Könyvkiadó Kft.}, unique-id = {3423867}, year = {2018} } @article{MTMT:3231445, title = {Combining the auxin-inducible degradation system with CRISPR/Cas9-based genome editing for the conditional depletion of endogenous Drosophila melanogaster proteins}, url = {https://m2.mtmt.hu/api/publication/3231445}, author = {Bence, Melinda and Jankovics, Ferenc and Lukacsovich, Tamás and Erdélyi, Miklós}, doi = {10.1111/febs.14042}, journal-iso = {FEBS J}, journal = {FEBS JOURNAL}, volume = {284}, unique-id = {3231445}, issn = {1742-464X}, abstract = {Inducible protein degradation techniques have considerable advantages over classical genetic approaches, which generate loss-of-function phenotypes at the gene or mRNA level. The plant-derived auxin-inducible degradation system (AID) is a promising technique which enables the degradation of target proteins tagged with the AID motif in nonplant cells. Here, we present a detailed characterization of this method employed during the adult oogenesis of Drosophila. Furthermore, with the help of CRISPR/Cas9-based genome editing, we improve the utility of the AID system in the conditional elimination of endogenously expressed proteins. We demonstrate that the AID system induces efficient and reversible protein depletion of maternally provided proteins both in the ovary and the early embryo. Moreover, the AID system provides a fine spatiotemporal control of protein degradation and allows for the generation of different levels of protein knockdown in a well-regulated manner. These features of the AID system enable the unraveling of the discrete phenotypes of genes with highly complex functions. We utilized this system to generate a conditional loss-of-function allele which allows for the specific degradation of the Vasa protein without affecting its alternative splice variant (solo) and the vasa intronic gene (vig). With the help of this special allele, we demonstrate that dramatic decrease of Vasa protein in the vitellarium does not influence the completion of oogenesis as well as the establishment of proper anteroposterior and dorsoventral polarity in the developing oocyte. Our study suggests that both the localization and the translation of gurken mRNA in the vitellarium is independent from Vasa.}, keywords = {LOCALIZATION; Drosophila melanogaster; polarity; OOGENESIS; FEMALE STERILE MUTATIONS; ANTERIOR-POSTERIOR; 2ND CHROMOSOME; GURKEN; DEGRON SYSTEM; BOX HELICASE VASA; MATERNAL-EFFECT MUTATIONS; Vasa; induced protein depletion; CRISPR/Cas9; auxin-inducible degradation}, year = {2017}, eissn = {1742-4658}, pages = {1056-1069} } @article{MTMT:2853634, title = {Multinucleated Giant Hemocytes Are Effector Cells in Cell-Mediated Immune Responses of Drosophila}, url = {https://m2.mtmt.hu/api/publication/2853634}, author = {Márkus, Róbert and Lerner, Zita and Honti, Viktor and Csordás, Gábor and Zsámboki, János and Cinege, Gyöngyi Ilona and Párducz, Árpád and Lukacsovich, Tamás and Kurucz, Judit Éva and Andó, István}, doi = {10.1159/000369618}, journal-iso = {J INNATE IMMUN}, journal = {JOURNAL OF INNATE IMMUNITY}, volume = {7}, unique-id = {2853634}, issn = {1662-811X}, abstract = {We identified and characterized a so far unrecognized cell type, dubbed the multinucleated giant hemocyte (MGH), in the ananassae subgroup of Drosophilidae. Here, we describe the functional and ultrastructural characteristics of this novel blood cell type as well as its characterization with a set of discriminative immunological markers. MGHs are encapsulating cells that isolate and kill the parasite without melanization. They share some properties with but differ considerably from lamellocytes, the encapsulating cells of Drosophila melanogaster, the broadly used model organism in studies of innate immunity. MGHs are nonproliferative effector cells that are derived from phagocytic cells of the sessile tissue and the circulation, but do not exhibit phagocytic activity. In contrast to lamellocytes, MGHs are gigantic cells with filamentous projections and contain many nuclei, which are the result of the fusion of several cells. Although the structure of lamellocytes and MGHs differ remarkably, their function in the elimination of parasites is similar, which is potentially the result of the convergent evolution of interactions between hosts and parasites in different geographic regions. MGHs are highly motile and share several features with mammalian multinucleated giant cells, a syncytium of macrophages formed during granulomatous inflammation. © 2015 S. Karger AG, Basel}, year = {2015}, eissn = {1662-8128}, pages = {340-353}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Andó, István/0000-0002-4648-9396} } @inbook{MTMT:2765598, title = {Vérsejt-differenciálódás vizsgálata a Drosophila melanogaster hematopoietikus kompartmentumaiban}, url = {https://m2.mtmt.hu/api/publication/2765598}, author = {Varga, Gergely István and Honti, Viktor and Csordás, Gábor and Jankovics, Ferenc and Márkus, Róbert and Lukacsovich, Tamás and Kurucz, Judit Éva and Andó, István}, booktitle = {A biológia jövője, a jövő biológusai}, unique-id = {2765598}, year = {2014}, pages = {103-113}, orcid-numbers = {Varga, Gergely István/0000-0001-9073-5788; Csordás, Gábor/0000-0001-6871-6839; Andó, István/0000-0002-4648-9396} } @article{MTMT:2421415, title = {Viability, Longevity, and Egg Production ofDrosophila melanogasterAre Regulatedby the miR-282 microRNA}, url = {https://m2.mtmt.hu/api/publication/2421415}, author = {Vilmos, Péter and Bujna, Ágnes and Szuperák, Milán and Havelda, Zoltán and Várallyay, Éva and Szabad, János and Kucerova, L and Somogyi, Kálmán and Kristó, Ildikó and Lukacsovich, Tamás and Jankovics, Ferenc and Henn, László and Erdélyi, Miklós}, doi = {10.1534/genetics.113.153585}, journal-iso = {GENETICS}, journal = {GENETICS}, volume = {195}, unique-id = {2421415}, issn = {0016-6731}, abstract = {The first microRNAs were discovered some 20 years ago, but only a small fraction of the microRNA-encoding genes have been described in detail yet. Here we report the molecular analysis of a computationally predicted Drosophila melanogaster microRNA gene, mir-282. We show that the mir-282 gene is the source of a 4.9 kb long primary transcript with a 5' cap and a 3' polyA sequence and a mature microRNA of about 25 bp. Our data strongly suggest the existence of an independent mir-282 gene conserved in holometabolic insects. We give evidence that the mir-282 locus encodes a functional transcript which influences viability, longevity, and egg production in Drosophila. We identify the nervous system-specific adenylate cyclase (rutabaga) as a target of miR-282 and assume that one of the main functions of mir-282 is the regulation of adenylate cyclase activity in the nervous system during metamorphosis.}, year = {2013}, eissn = {1943-2631}, pages = {469-480} } @article{MTMT:1920806, title = {Live imaging reveals that the Drosophila actin-binding ERM protein, moesin, co-localizes with the mitotic spindle}, url = {https://m2.mtmt.hu/api/publication/1920806}, author = {Vilmos, Péter and Jankovics, Ferenc and Szathmári, Margit and Lukacsovich, Tamás and Henn, László and Erdélyi, Miklós}, doi = {10.1016/j.ejcb.2009.05.006}, journal-iso = {EUR J CELL BIOL}, journal = {EUROPEAN JOURNAL OF CELL BIOLOGY}, volume = {88}, unique-id = {1920806}, issn = {0171-9335}, year = {2009}, eissn = {1618-1298}, pages = {609-619} } @article{MTMT:1286292, title = {A novel role for the Drosophila epsin (lqf): involvement in autophagy}, url = {https://m2.mtmt.hu/api/publication/1286292}, author = {Csikós, György and Lippai, Mónika and Lukacsovich, Tamás and Juhász, Gábor and Henn, László and Erdélyi, Miklós and Maróy, Péter and Sass, Miklós}, doi = {10.4161/auto.5.5.8168}, journal-iso = {AUTOPHAGY}, journal = {AUTOPHAGY}, volume = {5}, unique-id = {1286292}, issn = {1554-8627}, keywords = {ENDOCYTOSIS; PROGRAMMED CELL-DEATH; SACCHAROMYCES-CEREVISIAE; Autophagy; IMMUNOLOCALIZATION; RNAi; DEUBIQUITINATING ENZYME; MEDIATED ENDOCYTOSIS; SELF-DIGESTION; Liquid facets; HEAT REPEATS; PIERIS-BRASSICAE; ENTH DOMAIN; MAMESTRA-BRASSICAE; FAT-BODY CELLS}, year = {2009}, eissn = {1554-8635}, pages = {636-648}, orcid-numbers = {Csikós, György/0000-0002-5881-5363; Lippai, Mónika/0000-0002-7307-4233; Juhász, Gábor/0000-0001-8548-8874} } @article{MTMT:1915224, title = {Application of the dual-tagging gene trap method combined with a novel automatic selection system to identify genes involved in germ cell development in Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/1915224}, author = {Vilmos, Péter and Henn, László and Szatmári, Anna Mária and Lukacsovich, Tamás and Sipos, László Attila and Erdélyi, Miklós}, doi = {10.1556/ABiol.58.2007.Suppl.7}, journal-iso = {ACTA BIOL HUNG}, journal = {ACTA BIOLOGICA HUNGARICA (1983-2018)}, volume = {58}, unique-id = {1915224}, issn = {0236-5383}, year = {2007}, eissn = {1588-256X}, pages = {81-94} } @article{MTMT:1915010, title = {Nimrod, a Putative Phagocytosis Receptor With Egf Repeats in Drosophila Plasmatocytes}, url = {https://m2.mtmt.hu/api/publication/1915010}, author = {Kurucz, Judit Éva and Márkus, Róbert and Zsámboki, János and Medzihradszky F., Katalin and Darula, Zsuzsanna and Vilmos, Péter and Udvardy, Andor and Krausz, Ildikó and Lukacsovich, Tamás and Gateff, E and Zettervall, CJ and Hultmark, D and Andó, István}, doi = {10.1016/j.cub.2007.02.041}, journal-iso = {CURR BIOL}, journal = {CURRENT BIOLOGY}, volume = {17}, unique-id = {1915010}, issn = {0960-9822}, year = {2007}, eissn = {1879-0445}, pages = {649-654}, orcid-numbers = {Andó, István/0000-0002-4648-9396} }