TY - JOUR AU - Ábrahám, Andrea AU - Villanyi, Zoltan AU - Zsindely, Nóra AU - Nagy, Gábor AU - Szabó, Áron AU - Bodai, László AU - Henn, László AU - Boros, Imre Miklós TI - Despite its sequence identity with canonical H4, Drosophila H4r product is enriched at specific chromatin regions JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 12 PY - 2022 IS - 1 PG - 11 SN - 2045-2322 DO - 10.1038/s41598-022-09026-x UR - https://m2.mtmt.hu/api/publication/32741372 ID - 32741372 N1 - Funding Agency and Grant Number: University of SzegedEuropean Commission; National Research, Development and Innovation OfficeNational Research, Development & Innovation Office (NRDIO) - Hungary [OTKA-116372]; Ministry for National Economy of Hungary [GINOP-2.3.2-15-2016-00032]; Hungarian National Research, Development and Innovation OfficeNational Research, Development & Innovation Office (NRDIO) - Hungary [UNKP-19-4-SZTE-118, UNKP-20-5-SZTE-671, UNKP-21-5-SZTE-574]; Janos Bolyai Research Scholarship of the Hungarian Academy of SciencesHungarian Academy of Sciences [BO/902/19, BO/00522/19/8]; National Research, Development and Innovation Office, Young Researchers' Excellence Programme [OTKA-FK: FK132183] Funding text: Open access funding provided by University of Szeged. National Research, Development and Innovation Office [OTKA-116372]; Ministry for National Economy of Hungary [GINOP-2.3.2-15-2016-00032]. Z.V. was supported by Grants UNKP-19-4-SZTE-118 and UNKP-20-5-SZTE-671, L.B. by UNKP-21-5-SZTE-574 from the Hungarian National Research, Development and Innovation Office. Z.V. and L.B. received Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/902/19 and BO/00522/19/8, respectively) A.Sz. was supported by the National Research, Development and Innovation Office, Young Researchers' Excellence Programme (OTKA-FK: FK132183). Open acces charge provided by institutional basic funding. LA - English DB - MTMT ER - TY - JOUR AU - Climent-Cantó, Paula AU - Carbonell, Albert AU - Tamirisa, Srividya AU - Henn, László AU - Pérez-Montero, Salvador AU - Boros, Imre Miklós AU - Azorín, Fernando TI - The tumour suppressor brain tumour (Brat) regulates linker histone dBigH1 expression in the Drosophila female germline and the early embryo JF - OPEN BIOLOGY J2 - OPEN BIOL VL - 11 PY - 2021 IS - 5 PG - 13 SN - 2046-2441 DO - 10.1098/rsob.200408 UR - https://m2.mtmt.hu/api/publication/32000174 ID - 32000174 N1 - Cited By :1 Export Date: 10 March 2022 LA - English DB - MTMT ER - TY - GEN AU - Albert, Carbonell AU - Henn, László AU - Juan, Pérez-Roldán AU - Srividya, Tamirisa AU - Szabó, Anikó AU - Boros, Imre Miklós AU - Fernando, Azorín TI - In response to Li et al.: Linker histones function in Drosophila embryogenesis PY - 2021 SP - in press UR - https://m2.mtmt.hu/api/publication/31884635 ID - 31884635 LA - English DB - MTMT ER - TY - JOUR AU - Henn, László AU - Szabó, Anikó AU - Imre, László AU - Román, Ádám AU - Ábrahám, Andrea AU - Vedelek, Balázs AU - Nánási, Péter Pál AU - Boros, Imre Miklós TI - Alternative linker histone permits fast paced nuclear divisions in early Drosophila embryo JF - NUCLEIC ACIDS RESEARCH J2 - NUCLEIC ACIDS RES VL - 48 PY - 2020 IS - 16 SP - 9007 EP - 9018 PG - 12 SN - 0305-1048 DO - 10.1093/nar/gkaa624 UR - https://m2.mtmt.hu/api/publication/31397830 ID - 31397830 AB - In most animals, the start of embryogenesis requires specific histones. In Drosophila linker histone variant BigH1 is present in early embryos. To uncover the specific role of this alternative linker histone at early embryogenesis, we established fly lines in which domains of BigH1 have been replaced partially or completely with that of H1. Analysis of the resulting Drosophila lines revealed that at normal temperature somatic H1 can substitute the alternative linker histone, but at low temperature the globular and C-terminal domains of BigH1 are essential for embryogenesis. In the presence of BigH1 nucleosome stability increases and core histone incorporation into nucleosomes is more rapid, while nucleosome spacing is unchanged. Chromatin formation in the presence of BigH1 permits the fast-paced nuclear divisions of the early embryo. We propose a model which explains how this specific linker histone ensures the rapid nucleosome reassembly required during quick replication cycles at the start of embryogenesis. LA - English DB - MTMT ER - TY - GEN AU - Bence, Melinda AU - Jankovics, F AU - Nkubito, CK AU - Henn, László AU - Erdélyi, M TI - Role of Su(var)2-10 gene in germ cell development of Drosophila melanogaster PY - 2018 SP - 101 EP - 101 PG - 1 UR - https://m2.mtmt.hu/api/publication/3418685 ID - 3418685 N1 - Előadás, absztrakt LA - English DB - MTMT ER - TY - GEN AU - Bence, Melinda AU - Jankovics, F AU - Henn, László AU - Erdélyi, M TI - Role of sumoylation in germ cell development of Drosophila PY - 2017 SP - 84 EP - 84 PG - 1 UR - https://m2.mtmt.hu/api/publication/3418665 ID - 3418665 N1 - Előadás, absztrakt LA - English DB - MTMT ER - TY - CHAP AU - Jankovics, Ferenc AU - Henn, László AU - Vilmos, Péter ED - Vágvölgyi, Csaba ED - Siklós, László TI - Intracellular skeletal structures in eukaryotes T2 - Selected Topics from Contemporary Experimental Biology, Volume 2 PB - MTA Szegedi Biológiai Központ CY - Szeged PY - 2015 SP - 155 EP - 168 PG - 14 UR - https://m2.mtmt.hu/api/publication/3003054 ID - 3003054 LA - English DB - MTMT ER - TY - JOUR AU - Horváth, András AU - Batki, J AU - Henn, László AU - Lukacsovich, T AU - Róna, Gergely AU - Erdélyi, Miklós AU - Vértessy, Beáta (Grolmuszné) TI - dUTPase expression correlates with cell division potential in Drosophila melanogaster. JF - FEBS JOURNAL J2 - FEBS J VL - 282 PY - 2015 IS - 10 SP - 1998 EP - 2013 PG - 16 SN - 1742-464X DO - 10.1111/febs.13255 UR - https://m2.mtmt.hu/api/publication/2863483 ID - 2863483 N1 - Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary Institute of Genetics, Hungarian Academy of Sciences, Szeged, Hungary Department of Developmental and Cell Biology, University of California, Irvine, CA, United States Department of Applied Biotechnology and Food Sciences, Budapest University of Technology and Economics, Budapest, Hungary Cited By :1 Export Date: 6 May 2021 CODEN: FJEOA Correspondence Address: Horváth, A.; Institute of Enzymology, Hungary; email: horvath.andras@ttk.mta.hu Chemicals/CAS: deoxyuridine triphosphate pyrophosphatase, 37289-34-2; inorganic pyrophosphatase, 9024-82-2, 9033-44-7; dUTP pyrophosphatase; Pyrophosphatases Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary Institute of Genetics, Hungarian Academy of Sciences, Szeged, Hungary Department of Developmental and Cell Biology, University of California, Irvine, CA, United States Department of Applied Biotechnology and Food Sciences, Budapest University of Technology and Economics, Budapest, Hungary Cited By :1 Export Date: 7 May 2021 CODEN: FJEOA Correspondence Address: Horváth, A.; Institute of Enzymology, Hungary; email: horvath.andras@ttk.mta.hu Chemicals/CAS: deoxyuridine triphosphate pyrophosphatase, 37289-34-2; inorganic pyrophosphatase, 9024-82-2, 9033-44-7; dUTP pyrophosphatase; Pyrophosphatases Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary Institute of Genetics, Hungarian Academy of Sciences, Szeged, Hungary Department of Developmental and Cell Biology, University of California, Irvine, CA, United States Department of Applied Biotechnology and Food Sciences, Budapest University of Technology and Economics, Budapest, Hungary Cited By :1 Export Date: 10 May 2021 CODEN: FJEOA Correspondence Address: Horváth, A.; Institute of Enzymology, Hungary; email: horvath.andras@ttk.mta.hu Chemicals/CAS: deoxyuridine triphosphate pyrophosphatase, 37289-34-2; inorganic pyrophosphatase, 9024-82-2, 9033-44-7; dUTP pyrophosphatase; Pyrophosphatases Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary Institute of Genetics, Hungarian Academy of Sciences, Szeged, Hungary Department of Developmental and Cell Biology, University of California, Irvine, CA, United States Department of Applied Biotechnology and Food Sciences, Budapest University of Technology and Economics, Budapest, Hungary Cited By :1 Export Date: 11 May 2021 CODEN: FJEOA Correspondence Address: Horváth, A.; Institute of Enzymology, Hungary; email: horvath.andras@ttk.mta.hu Chemicals/CAS: deoxyuridine triphosphate pyrophosphatase, 37289-34-2; inorganic pyrophosphatase, 9024-82-2, 9033-44-7; dUTP pyrophosphatase; Pyrophosphatases Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary Institute of Genetics, Hungarian Academy of Sciences, Szeged, Hungary Department of Developmental and Cell Biology, University of California, Irvine, CA, United States Department of Applied Biotechnology and Food Sciences, Budapest University of Technology and Economics, Budapest, Hungary Cited By :1 Export Date: 12 May 2021 CODEN: FJEOA Correspondence Address: Horváth, A.; Institute of Enzymology, Hungary; email: horvath.andras@ttk.mta.hu Chemicals/CAS: deoxyuridine triphosphate pyrophosphatase, 37289-34-2; inorganic pyrophosphatase, 9024-82-2, 9033-44-7; dUTP pyrophosphatase; Pyrophosphatases Funding Agency and Grant Number: Hungarian Scientific Research FundOrszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [OTKA NK 84008, OTKA K109486]; Baross program of the New Hungary Development Plan (3DSTRUCT) [OMFB-00266/2010 REG-KM-09-1-2009-0050]; Hungarian Academy of SciencesHungarian Academy of Sciences [TTK IF-28/2012]; MedinProt program of the Hungarian Academy of Sciences; European CommissionEuropean CommissionEuropean Commission Joint Research Centre [283570]; Hungarian Academy of SciencesHungarian Academy of Sciences Funding text: This work was supported by grants from the by the Hungarian Scientific Research Fund (OTKA NK 84008, OTKA K109486), the Baross program of the New Hungary Development Plan (3DSTRUCT, OMFB-00266/2010 REG-KM-09-1-2009-0050), the Hungarian Academy of Sciences (TTK IF-28/2012), the MedinProt program of the Hungarian Academy of Sciences and the European Commission FP7 BioStruct-X project (contract No. 283570), to BGV. AH and GR are recipients of Young Researcher Fellowships from the Hungarian Academy of Sciences. AB - dUTPase is a dNTP sanitizing enzyme that prevents the appearance of the potentially harmful uracil bases in DNA by hydrolyzing cellular dUTP. This function of dUTPase is found to be essential in many organisms including Drosophila melanogaster. Previously we showed that the expression pattern of dUTPase determines the extent of uracil accumulation in the genome of different tissues. We wished to reveal the regulatory mechanism that eventually leaves a set of tissues to have uracil-free and intact genome. We found that the expression pattern established by the promoter of Drosophila dUTPase overlaps with mRNA and protein expression pattern, excluding the involvement of other posttranscriptional contribution. This promoter was found to be active in primordial tissues, such as in imaginal discs of the larvae, in the larval brain and in reproductive organs. In the case of brain and imaginal tissues, we observed that the promoter activity depends on DRE motifs, the docking site of DREF, which is known as a transcriptional activator of genes involved in replication and proliferation. These results suggest that dUTPase expression is fine-tuned to meet the requirements of DNA synthesis, in tissues where the maintenance of genome integrity is of high importance. This article is protected by copyright. All rights reserved. LA - English DB - MTMT ER - TY - THES AU - Henn, László TI - A Drosophila melanogaster ivarvonal-fejlődésében szerepet játszó gének azonosítása és vizsgálata PB - Szegedi Tudományegyetem (SZTE) PY - 2014 SP - 135 DO - 10.14232/phd.2271 UR - https://m2.mtmt.hu/api/publication/2778905 ID - 2778905 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Jankovics, Ferenc AU - Henn, László AU - Bujna, Ágnes AU - Vilmos, Péter AU - Spirohn, K AU - Boutros, M AU - Erdélyi, Miklós TI - Functional analysis of the Drosophila embryonic germ cell transcriptome by RNA interference. JF - PLOS ONE J2 - PLOS ONE VL - 9 PY - 2014 IS - 6 PG - 12 SN - 1932-6203 DO - 10.1371/journal.pone.0098579 UR - https://m2.mtmt.hu/api/publication/2599598 ID - 2599598 N1 - Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary German Cancer Research Center (DKFZ), Heidelberg University, Division Signaling and Functional Genomics, Heidelberg, Germany Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, United States Cited By :6 Export Date: 20 April 2021 CODEN: POLNC AB - In Drosophila melanogaster, primordial germ cells are specified at the posterior pole of the very early embryo. This process is regulated by the posterior localized germ plasm that contains a large number of RNAs of maternal origin. Transcription in the primordial germ cells is actively down-regulated until germ cell fate is established. Bulk expression of the zygotic genes commences concomitantly with the degradation of the maternal transcripts. Thus, during embryogenesis, maternally provided and zygotically transcribed mRNAs determine germ cell development collectively. In an effort to identify novel genes involved in the regulation of germ cell behavior, we carried out a large-scale RNAi screen targeting both maternal and zygotic components of the embryonic germ line transcriptome. We identified 48 genes necessary for distinct stages in germ cell development. We found pebble and fascetto to be essential for germ cell migration and germ cell division, respectively. Our data uncover a previously unanticipated role of mei-P26 in maintenance of embryonic germ cell fate. We also performed systematic co-RNAi experiments, through which we found a low rate of functional redundancy among homologous gene pairs. As our data indicate a high degree of evolutionary conservation in genetic regulation of germ cell development, they are likely to provide valuable insights into the biology of the germ line in general. LA - English DB - MTMT ER -