@article{MTMT:32741372, title = {Despite its sequence identity with canonical H4, Drosophila H4r product is enriched at specific chromatin regions}, url = {https://m2.mtmt.hu/api/publication/32741372}, author = {Ábrahám, Andrea and Villanyi, Zoltan and Zsindely, Nóra and Nagy, Gábor and Szabó, Áron and Bodai, László and Henn, László and Boros, Imre Miklós}, doi = {10.1038/s41598-022-09026-x}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {12}, unique-id = {32741372}, issn = {2045-2322}, year = {2022}, eissn = {2045-2322}, orcid-numbers = {Zsindely, Nóra/0000-0002-6189-3100; Nagy, Gábor/0000-0001-5464-1135; Bodai, László/0000-0001-8411-626X; Boros, Imre Miklós/0000-0001-8504-9687} } @article{MTMT:32000174, title = {The tumour suppressor brain tumour (Brat) regulates linker histone dBigH1 expression in the Drosophila female germline and the early embryo}, url = {https://m2.mtmt.hu/api/publication/32000174}, author = {Climent-Cantó, Paula and Carbonell, Albert and Tamirisa, Srividya and Henn, László and Pérez-Montero, Salvador and Boros, Imre Miklós and Azorín, Fernando}, doi = {10.1098/rsob.200408}, journal-iso = {OPEN BIOL}, journal = {OPEN BIOLOGY}, volume = {11}, unique-id = {32000174}, year = {2021}, eissn = {2046-2441}, orcid-numbers = {Carbonell, Albert/0000-0002-1949-232X; Boros, Imre Miklós/0000-0001-8504-9687; Azorín, Fernando/0000-0002-8426-7858} } @misc{MTMT:31884635, title = {In response to Li et al.: Linker histones function in Drosophila embryogenesis}, url = {https://m2.mtmt.hu/api/publication/31884635}, author = {Albert, Carbonell and Henn, László and Juan, Pérez-Roldán and Srividya, Tamirisa and Szabó, Anikó and Boros, Imre Miklós and Fernando, Azorín}, unique-id = {31884635}, year = {2021}, pages = {in press}, orcid-numbers = {Boros, Imre Miklós/0000-0001-8504-9687} } @article{MTMT:31397830, title = {Alternative linker histone permits fast paced nuclear divisions in early Drosophila embryo}, url = {https://m2.mtmt.hu/api/publication/31397830}, author = {Henn, László and Szabó, Anikó and Imre, László and Román, Ádám and Ábrahám, Andrea and Vedelek, Balázs and Nánási, Péter Pál and Boros, Imre Miklós}, doi = {10.1093/nar/gkaa624}, journal-iso = {NUCLEIC ACIDS RES}, journal = {NUCLEIC ACIDS RESEARCH}, volume = {48}, unique-id = {31397830}, issn = {0305-1048}, abstract = {In most animals, the start of embryogenesis requires specific histones. In Drosophila linker histone variant BigH1 is present in early embryos. To uncover the specific role of this alternative linker histone at early embryogenesis, we established fly lines in which domains of BigH1 have been replaced partially or completely with that of H1. Analysis of the resulting Drosophila lines revealed that at normal temperature somatic H1 can substitute the alternative linker histone, but at low temperature the globular and C-terminal domains of BigH1 are essential for embryogenesis. In the presence of BigH1 nucleosome stability increases and core histone incorporation into nucleosomes is more rapid, while nucleosome spacing is unchanged. Chromatin formation in the presence of BigH1 permits the fast-paced nuclear divisions of the early embryo. We propose a model which explains how this specific linker histone ensures the rapid nucleosome reassembly required during quick replication cycles at the start of embryogenesis.}, year = {2020}, eissn = {1362-4962}, pages = {9007-9018}, orcid-numbers = {Román, Ádám/0000-0003-2007-3269; Vedelek, Balázs/0000-0001-6981-0026; Boros, Imre Miklós/0000-0001-8504-9687} } @misc{MTMT:3418685, title = {Role of Su(var)2-10 gene in germ cell development of Drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/3418685}, author = {Bence, Melinda and Jankovics, F and Nkubito, CK and Henn, László and Erdélyi, M}, unique-id = {3418685}, year = {2018}, pages = {101-101} } @misc{MTMT:3418665, title = {Role of sumoylation in germ cell development of Drosophila}, url = {https://m2.mtmt.hu/api/publication/3418665}, author = {Bence, Melinda and Jankovics, F and Henn, László and Erdélyi, M}, unique-id = {3418665}, year = {2017}, pages = {84-84} } @{MTMT:3003054, title = {Intracellular skeletal structures in eukaryotes}, url = {https://m2.mtmt.hu/api/publication/3003054}, author = {Jankovics, Ferenc and Henn, László and Vilmos, Péter}, booktitle = {Selected Topics from Contemporary Experimental Biology, Volume 2}, unique-id = {3003054}, year = {2015}, pages = {155-168} } @article{MTMT:2863483, title = {dUTPase expression correlates with cell division potential in Drosophila melanogaster.}, url = {https://m2.mtmt.hu/api/publication/2863483}, author = {Horváth, András and Batki, J and Henn, László and Lukacsovich, T and Róna, Gergely and Erdélyi, Miklós and Vértessy, Beáta (Grolmuszné)}, doi = {10.1111/febs.13255}, journal-iso = {FEBS J}, journal = {FEBS JOURNAL}, volume = {282}, unique-id = {2863483}, issn = {1742-464X}, abstract = {dUTPase is a dNTP sanitizing enzyme that prevents the appearance of the potentially harmful uracil bases in DNA by hydrolyzing cellular dUTP. This function of dUTPase is found to be essential in many organisms including Drosophila melanogaster. Previously we showed that the expression pattern of dUTPase determines the extent of uracil accumulation in the genome of different tissues. We wished to reveal the regulatory mechanism that eventually leaves a set of tissues to have uracil-free and intact genome. We found that the expression pattern established by the promoter of Drosophila dUTPase overlaps with mRNA and protein expression pattern, excluding the involvement of other posttranscriptional contribution. This promoter was found to be active in primordial tissues, such as in imaginal discs of the larvae, in the larval brain and in reproductive organs. In the case of brain and imaginal tissues, we observed that the promoter activity depends on DRE motifs, the docking site of DREF, which is known as a transcriptional activator of genes involved in replication and proliferation. These results suggest that dUTPase expression is fine-tuned to meet the requirements of DNA synthesis, in tissues where the maintenance of genome integrity is of high importance. This article is protected by copyright. All rights reserved.}, year = {2015}, eissn = {1742-4658}, pages = {1998-2013}, orcid-numbers = {Róna, Gergely/0000-0003-3222-7261} } @mastersthesis{MTMT:2778905, title = {A Drosophila melanogaster ivarvonal-fejlődésében szerepet játszó gének azonosítása és vizsgálata}, url = {https://m2.mtmt.hu/api/publication/2778905}, author = {Henn, László}, doi = {10.14232/phd.2271}, publisher = {SZTE}, unique-id = {2778905}, year = {2014} } @article{MTMT:2599598, title = {Functional analysis of the Drosophila embryonic germ cell transcriptome by RNA interference.}, url = {https://m2.mtmt.hu/api/publication/2599598}, author = {Jankovics, Ferenc and Henn, László and Bujna, Ágnes and Vilmos, Péter and Spirohn, K and Boutros, M and Erdélyi, Miklós}, doi = {10.1371/journal.pone.0098579}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {9}, unique-id = {2599598}, issn = {1932-6203}, abstract = {In Drosophila melanogaster, primordial germ cells are specified at the posterior pole of the very early embryo. This process is regulated by the posterior localized germ plasm that contains a large number of RNAs of maternal origin. Transcription in the primordial germ cells is actively down-regulated until germ cell fate is established. Bulk expression of the zygotic genes commences concomitantly with the degradation of the maternal transcripts. Thus, during embryogenesis, maternally provided and zygotically transcribed mRNAs determine germ cell development collectively. In an effort to identify novel genes involved in the regulation of germ cell behavior, we carried out a large-scale RNAi screen targeting both maternal and zygotic components of the embryonic germ line transcriptome. We identified 48 genes necessary for distinct stages in germ cell development. We found pebble and fascetto to be essential for germ cell migration and germ cell division, respectively. Our data uncover a previously unanticipated role of mei-P26 in maintenance of embryonic germ cell fate. We also performed systematic co-RNAi experiments, through which we found a low rate of functional redundancy among homologous gene pairs. As our data indicate a high degree of evolutionary conservation in genetic regulation of germ cell development, they are likely to provide valuable insights into the biology of the germ line in general.}, year = {2014}, eissn = {1932-6203} }