TY - JOUR AU - Laczkó-Dobos, Hajnalka AU - Bhattacharjee, Arindam AU - Maddali, Asha Kiran AU - Kincses, András AU - Abuammar, Hussein AU - Sebőkné Nagy, Krisztina AU - Páli, Tibor AU - Dér, András AU - Hegedűs, Tamás AU - Csordás, Gábor AU - Juhász, Gábor TI - PtdIns4p is required for the autophagosomal recruitment of STX17 (syntaxin 17) to promote lysosomal fusion JF - AUTOPHAGY J2 - AUTOPHAGY VL - AiP PY - 2024 PG - 12 SN - 1554-8627 DO - 10.1080/15548627.2024.2322493 UR - https://m2.mtmt.hu/api/publication/34724664 ID - 34724664 N1 - Institute of Genetics, HUN-REN Biological Research Centre Szeged, Szeged, Hungary Doctoral School of Biology, University of Szeged, Szeged, Hungary Institute of Biophysics, HUN-REN Biological Research Centre Szeged, Szeged, Hungary Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary HUN-REN Biophysical Virology Research Group, Budapest, Hungary Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary Export Date: 27 March 2024 Correspondence Address: Juhász, G.; HUN-REN Biological Research Centre Szeged, Temesvari krt. 62, Hungary; email: juhasz.gabor@brc.hu LA - English DB - MTMT ER - TY - JOUR AU - Kúthy-Sutus, Enikő AU - Kharrat, Bayan AU - Gábor, Erika AU - Csordás, Gábor AU - Sinka, Rita AU - Honti, Viktor TI - A Novel Method for Primary Blood Cell Culturing and Selection in Drosophila melanogaster JF - CELLS J2 - CELLS-BASEL VL - 12 PY - 2023 IS - 1 PG - 15 SN - 2073-4409 DO - 10.3390/cells12010024 UR - https://m2.mtmt.hu/api/publication/33555087 ID - 33555087 N1 - Export Date: 24 January 2023 AB - The blood cells of the fruit fly Drosophila melanogaster show many similarities to their vertebrate counterparts, both in their functions and their differentiation. In the past decades, a wide palette of immunological and transgenic tools and methods have been developed to study hematopoiesis in the Drosophila larva. However, the in vivo observation of blood cells is technically restricted by the limited transparency of the body and the difficulty in keeping the organism alive during imaging. Here we describe an improved ex vivo culturing method that allows effective visualization and selection of live blood cells in primary cultures derived from Drosophila larvae. Our results show that cultured hemocytes accurately represent morphological and functional changes following immune challenges and in case of genetic alterations. Since cell culturing has hugely contributed to the understanding of the physiological properties of vertebrate blood cells, this method provides a versatile tool for studying Drosophila hemocyte differentiation and functions ex vivo. LA - English DB - MTMT ER - TY - JOUR AU - Stanković, Dimitrije AU - Csordás, Gábor AU - Uhlirova, Mirka TI - Drosophila pVALIUM10 TRiP RNAi lines cause undesired silencing of Gateway-based transgenes JF - LIFE SCIENCE ALLIANCE J2 - LIFE SCI ALLIANCE VL - 6 PY - 2022 IS - 2 SN - 2575-1077 DO - 10.26508/lsa.202201801 UR - https://m2.mtmt.hu/api/publication/33288064 ID - 33288064 N1 - Funding Agency and Grant Number: NIH [2P40OD010949]; Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy -CECAD [EXC 2030 -390661388] Funding text: We thank Norbert Perrimon for discussions and comments on the article. We thank the Bloomington Drosophila Stock Center (BDSC, Bloomington, IN, USA), the Vienna Drosophila Resource Center (VDRC, Vienna, Austria), the Drosophila Genomic Resource Center (DGRC, supported by NIH grant 2P40OD010949, Bloomington, IN, USA), and the Zurich ORFeome Project (FlyORF, Zurich, Switzerland) for fly stocks and plasmids. We are grateful to Tina Bresser for generation of transgenic lines and Nils Teuscher for fly stock maintenance and technical assistance. This work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy -CECAD (EXC 2030 -390661388). AB - Post-transcriptional gene silencing using double-stranded RNA has revolutionized the field of functional genetics, allowing fast and easy disruption of gene function in various organisms. In Drosophila , many transgenic RNAi lines have been generated in large-scale efforts, including the Drosophila Transgenic RNAi Project (TRiP), to facilitate in vivo knockdown of virtually any Drosophila gene with spatial and temporal resolution. The available transgenic RNAi lines represent a fundamental resource for the fly community, providing an unprecedented opportunity to address a vast range of biological questions relevant to basic and biomedical research fields. However, caution should be applied regarding the efficiency and specificity of the RNAi approach. Here, we demonstrate that pVALIUM10-based RNAi lines, representing ∼13% of the total TRiP collection (1,808 of 13,410 pVALIUM TRiP–based RNAi lines), cause unintended off-target silencing of transgenes expressed from Gateway destination vectors. The silencing is mediated by targeting attB1 and attB2 sequences generated via site-specific recombination and included in the transcribed mRNA. Deleting these attB sites from the Gateway expression vector prevents silencing and restores expected transgene expression. LA - English DB - MTMT ER - TY - JOUR AU - Kharrat, Bayan AU - Csordás, Gábor AU - Honti, Viktor TI - Peeling Back the Layers of Lymph Gland Structure and Regulation JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 14 PG - 16 SN - 1661-6596 DO - 10.3390/ijms23147767 UR - https://m2.mtmt.hu/api/publication/33050458 ID - 33050458 N1 - Cited By :2 Export Date: 24 January 2023 AB - During the past 60 years, the fruit fly, Drosophila melanogaster, has proven to be an excellent model to study the regulation of hematopoiesis. This is not only due to the evolutionarily conserved signalling pathways and transcription factors contributing to blood cell fate, but also to convergent evolution that led to functional similarities in distinct species. An example of convergence is the compartmentalization of blood cells, which ensures the quiescence of hematopoietic stem cells and allows for the rapid reaction of the immune system upon challenges. The lymph gland, a widely studied hematopoietic organ of the Drosophila larva, represents a microenvironment with similar features and functions to classical hematopoietic stem cell niches of vertebrates. Lymph gland studies were effectively supported by the unparalleled toolkit developed in Drosophila, which enabled the high-resolution investigation of the cellular composition and regulatory interaction networks of the lymph gland. In this review, we summarize how our understanding of lymph gland structure and hematopoietic cell-to-cell communication evolved during the past decades and compare their analogous features to those of the vertebrate hematopoietic stem cell niche. LA - English DB - MTMT ER - TY - JOUR AU - Gohel, Raksha AU - Rahman, Ashrafur AU - Lőrincz, Péter AU - Nagy, Anikó Zsuzsanna AU - Csordás, Gábor AU - Zhang, Yan AU - Juhász, Gábor AU - Nezis, Ioannis P. TI - Selective autophagy and Golgi quality control in Drosophila JF - AUTOPHAGY J2 - AUTOPHAGY VL - 18 PY - 2022 IS - 10 SP - 2508 EP - 2509 PG - 2 SN - 1554-8627 DO - 10.1080/15548627.2022.2098765 UR - https://m2.mtmt.hu/api/publication/33011261 ID - 33011261 N1 - Funding Agency and Grant Number: BBSRC [BB/L006324/1, BB/P007856/1, BB/V014838/1]; Leverhulme Trust [RPG-2017-023] Funding text: This work was supported by BBSRC grants BB/L006324/1, BB/P007856/1, BB/V014838/1 and Leverhulme Trust Project grant RPG-2017-023 awarded to I.P.N. AB - The LIR motif-docking site (LDS) of Atg8/LC3 proteins is essential for the binding of LC3-interacting region (LIR)-containing proteins and their subsequent degradation by macroautophagy/autophagy. In our recent study, we created a mutated LDS site in Atg8a, the Drosophila homolog of Atg8/LC3 and found that LDS mutants accumulate known autophagy substrates and have reduced lifespan. We also conducted quantitative proteomics analyses and identified several proteins that are enriched in the LDS mutants, including Gmap (Golgi microtubule-associated protein). Gmap contains a LIR motif and accumulates in LDS mutants. We showed that Gmap and Atg8a interact in a LIR-LDS dependent manner and that the Golgi size and morphology are altered in Atg8a-LDS and Gmap-LIR motif mutants. Our findings highlight a role for Gmap in the regulation of Golgiphagy. LA - English DB - MTMT ER - TY - JOUR AU - Rahman, Ashrafur AU - Lőrincz, Péter AU - Gohel, Raksha AU - Nagy, Anikó Zsuzsanna AU - Csordás, Gábor AU - Zhang, Yan AU - Juhász, Gábor AU - Nezis, Ioannis P. TI - GMAP is an Atg8a-interacting protein that regulates Golgi turnover in Drosophila JF - CELL REPORTS J2 - CELL REP VL - 39 PY - 2022 IS - 9 PG - 14 SN - 2211-1247 DO - 10.1016/j.celrep.2022.110903 UR - https://m2.mtmt.hu/api/publication/32876121 ID - 32876121 N1 - Export Date: 4 July 2022 LA - English DB - MTMT ER - TY - JOUR AU - Külshammer, Eva AU - Kilinc, Merve AU - Csordás, Gábor AU - Bresser, Tina AU - Nolte, Hendrik AU - Uhlirova, Mirka TI - The mechanosensor Filamin A/Cheerio promotes tumorigenesis via specific interactions with components of the cell cortex JF - FEBS JOURNAL J2 - FEBS J VL - 289 PY - 2022 SP - 4497 EP - 4517 PG - 21 SN - 1742-464X DO - 10.1111/febs.16408 UR - https://m2.mtmt.hu/api/publication/32697766 ID - 32697766 N1 - Export Date: 14 June 2022 CODEN: FJEOA AB - Cancer development has been linked to aberrant sensing and interpretation of mechanical cues and force-generating properties. Here, we show that upregulation of the actin crosslinking protein Cheerio (Cher), the fly ortholog of Filamin A (FLNA), and the conformation of its mechanosensitive region (MSR) are instrumental to the malignancy of polarity-deficient, Ras-driven tumours in Drosophila epithelia. We demonstrate that impaired growth and cytoskeletal contractility of tumours devoid of cher can be rescued by stimulating myosin activity. Profiling the Cher interactome in tumour-bearing imaginal discs identified several components of the cell cortex, including the β-heavy Spectrin Karst (Kst), the scaffolding protein Big bang (Bbg), and 14-3-3ε. We show that Cher binds Bbg through the MSR while the interaction with 14-3-3ε and Kst is MSR-independent. Importantly, our genetic studies define Bbg, Kst, and 14-3-3ε as tumour suppressors. The tumour-promoting function of Cher thus relies on its capacity to control the contractile state of the cytoskeleton through interactions with myosin and specific components of the cell cortex. © 2022 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies LA - English DB - MTMT ER - TY - JOUR AU - Abuammar, Hussein AU - Bhattacharjee, Arindam AU - Simon-Vecsei, Zsófia Judit AU - Blastyák, András AU - Csordás, Gábor AU - Páli, Tibor AU - Juhász, Gábor TI - Ion Channels and Pumps in Autophagy: A Reciprocal Relationship JF - CELLS J2 - CELLS-BASEL VL - 10 PY - 2021 IS - 12 SN - 2073-4409 DO - 10.3390/cells10123537 UR - https://m2.mtmt.hu/api/publication/32543729 ID - 32543729 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office of HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [KKP129797, 2018-1.2.1-NKP-2018-00005, NKFIH-871-3/2020, PD135587] Funding text: Work in the Juhasz lab is funded by the National Research, Development and Innovation Office of Hungary (KKP129797, 2018-1.2.1-NKP-2018-00005, NKFIH-871-3/2020 and PD135587). LA - English DB - MTMT ER - TY - JOUR AU - Csordás, Gábor AU - Gábor, Erika AU - Honti, Viktor TI - There and back again: The mechanisms of differentiation and transdifferentiation in Drosophila blood cells JF - DEVELOPMENTAL BIOLOGY J2 - DEV BIOL VL - 469 PY - 2021 SP - 135 EP - 143 PG - 9 SN - 0012-1606 DO - 10.1016/j.ydbio.2020.10.006 UR - https://m2.mtmt.hu/api/publication/31743832 ID - 31743832 N1 - Cited By :5 Export Date: 14 June 2022 CODEN: DEBIA LA - English DB - MTMT ER - TY - JOUR AU - Csordás, Gábor AU - Grawe, Ferdinand AU - Uhlirova, Mirka TI - Eater cooperates with Multiplexin to drive the formation of hematopoietic compartments JF - ELIFE J2 - ELIFE VL - 9 PY - 2020 PG - 27 SN - 2050-084X DO - 10.7554/eLife.57297 UR - https://m2.mtmt.hu/api/publication/31686151 ID - 31686151 N1 - Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany Molecular Cell Biology, Institute I for Anatomy, University of Cologne Medical School, Cologne, Germany Export Date: 8 March 2021 Correspondence Address: Csordás, G.; Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Germany; email: cgabor@uni-koeln.de Correspondence Address: Uhlirova, M.; Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Germany; email: mirka.uhlirova@uni-koeln.de Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany Molecular Cell Biology, Institute I for Anatomy, University of Cologne Medical School, Cologne, Germany Cited By :1 Export Date: 10 May 2021 Correspondence Address: Csordás, G.; Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Germany; email: cgabor@uni-koeln.de Correspondence Address: Uhlirova, M.; Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Germany; email: mirka.uhlirova@uni-koeln.de AB - Blood development in multicellular organisms relies on specific tissue microenvironments that nurture hematopoietic precursors and promote their self-renewal, proliferation, and differentiation. The mechanisms driving blood cell homing and their interactions with hematopoietic microenvironments remain poorly understood. Here, we use the Drosophila melanogaster model to reveal a pivotal role for basement membrane composition in the formation of hematopoietic compartments. We demonstrate that by modulating extracellular matrix components, the fly blood cells known as hemocytes can be relocated to tissue surfaces where they function similarly to their natural hematopoietic environment. We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association. These results highlight the cooperation of Multiplexin and Eater as an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila LA - English DB - MTMT ER -