@article{MTMT:34724664,
	title = {PtdIns4p is required for the autophagosomal recruitment of STX17 (syntaxin 17) to promote lysosomal fusion},
	url = {https://m2.mtmt.hu/api/publication/34724664},
	author = {Laczkó-Dobos, Hajnalka and Bhattacharjee, Arindam and Maddali, Asha Kiran and Kincses, András and Abuammar, Hussein and Sebőkné Nagy, Krisztina and Páli, Tibor and Dér, András and Hegedűs, Tamás and Csordás, Gábor and Juhász, Gábor},
	doi = {10.1080/15548627.2024.2322493},
	journal-iso = {AUTOPHAGY},
	journal = {AUTOPHAGY},
	volume = {AiP},
	unique-id = {34724664},
	issn = {1554-8627},
	year = {2024},
	eissn = {1554-8635},
	orcid-numbers = {Páli, Tibor/0000-0003-1649-1097; Hegedűs, Tamás/0000-0002-0331-9629; Csordás, Gábor/0000-0001-6871-6839; Juhász, Gábor/0000-0001-8548-8874}
}

@article{MTMT:33555087,
	title = {A Novel Method for Primary Blood Cell Culturing and Selection in Drosophila melanogaster},
	url = {https://m2.mtmt.hu/api/publication/33555087},
	author = {Kúthy-Sutus, Enikő and Kharrat, Bayan and Gábor, Erika and Csordás, Gábor and Sinka, Rita and Honti, Viktor},
	doi = {10.3390/cells12010024},
	journal-iso = {CELLS-BASEL},
	journal = {CELLS},
	volume = {12},
	unique-id = {33555087},
	abstract = {The blood cells of the fruit fly Drosophila melanogaster show many similarities to their vertebrate counterparts, both in their functions and their differentiation. In the past decades, a wide palette of immunological and transgenic tools and methods have been developed to study hematopoiesis in the Drosophila larva. However, the in vivo observation of blood cells is technically restricted by the limited transparency of the body and the difficulty in keeping the organism alive during imaging. Here we describe an improved ex vivo culturing method that allows effective visualization and selection of live blood cells in primary cultures derived from Drosophila larvae. Our results show that cultured hemocytes accurately represent morphological and functional changes following immune challenges and in case of genetic alterations. Since cell culturing has hugely contributed to the understanding of the physiological properties of vertebrate blood cells, this method provides a versatile tool for studying Drosophila hemocyte differentiation and functions ex vivo.},
	year = {2023},
	eissn = {2073-4409},
	orcid-numbers = {Kúthy-Sutus, Enikő/0000-0002-1398-4120; Csordás, Gábor/0000-0001-6871-6839; Sinka, Rita/0000-0003-4040-4184}
}

@article{MTMT:33288064,
	title = {Drosophila pVALIUM10 TRiP RNAi lines cause undesired silencing of Gateway-based transgenes},
	url = {https://m2.mtmt.hu/api/publication/33288064},
	author = {Stanković, Dimitrije and Csordás, Gábor and Uhlirova, Mirka},
	doi = {10.26508/lsa.202201801},
	journal-iso = {LIFE SCI ALLIANCE},
	journal = {LIFE SCIENCE ALLIANCE},
	volume = {6},
	unique-id = {33288064},
	abstract = {Post-transcriptional gene silencing using double-stranded RNA has revolutionized the field of functional genetics, allowing fast and easy disruption of gene function in various organisms. In Drosophila , many transgenic RNAi lines have been generated in large-scale efforts, including the Drosophila Transgenic RNAi Project (TRiP), to facilitate in vivo knockdown of virtually any Drosophila gene with spatial and temporal resolution. The available transgenic RNAi lines represent a fundamental resource for the fly community, providing an unprecedented opportunity to address a vast range of biological questions relevant to basic and biomedical research fields. However, caution should be applied regarding the efficiency and specificity of the RNAi approach. Here, we demonstrate that pVALIUM10-based RNAi lines, representing ∼13% of the total TRiP collection (1,808 of 13,410 pVALIUM TRiP–based RNAi lines), cause unintended off-target silencing of transgenes expressed from Gateway destination vectors. The silencing is mediated by targeting attB1 and attB2 sequences generated via site-specific recombination and included in the transcribed mRNA. Deleting these attB sites from the Gateway expression vector prevents silencing and restores expected transgene expression.},
	year = {2022},
	eissn = {2575-1077},
	orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Uhlirova, Mirka/0000-0002-5735-8287}
}

@article{MTMT:33050458,
	title = {Peeling Back the Layers of Lymph Gland Structure and Regulation},
	url = {https://m2.mtmt.hu/api/publication/33050458},
	author = {Kharrat, Bayan and Csordás, Gábor and Honti, Viktor},
	doi = {10.3390/ijms23147767},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {23},
	unique-id = {33050458},
	issn = {1661-6596},
	abstract = {During the past 60 years, the fruit fly, Drosophila melanogaster, has proven to be an excellent model to study the regulation of hematopoiesis. This is not only due to the evolutionarily conserved signalling pathways and transcription factors contributing to blood cell fate, but also to convergent evolution that led to functional similarities in distinct species. An example of convergence is the compartmentalization of blood cells, which ensures the quiescence of hematopoietic stem cells and allows for the rapid reaction of the immune system upon challenges. The lymph gland, a widely studied hematopoietic organ of the Drosophila larva, represents a microenvironment with similar features and functions to classical hematopoietic stem cell niches of vertebrates. Lymph gland studies were effectively supported by the unparalleled toolkit developed in Drosophila, which enabled the high-resolution investigation of the cellular composition and regulatory interaction networks of the lymph gland. In this review, we summarize how our understanding of lymph gland structure and hematopoietic cell-to-cell communication evolved during the past decades and compare their analogous features to those of the vertebrate hematopoietic stem cell niche.},
	keywords = {IMMUNE-RESPONSE; DROSOPHILA; matrix protein; Hematopoiesis; SELF-RENEWAL; HEMATOPOIETIC STEM-CELL; Biochemistry & Molecular Biology; HSC; N-cadherin; lymph gland; EMBRYONIC ORIGIN; Drosophila larvae; PROGENITOR MAINTENANCE; HEMOCYTE LINEAGES},
	year = {2022},
	eissn = {1422-0067},
	orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839}
}

@article{MTMT:33011261,
	title = {Selective autophagy and Golgi quality control in Drosophila},
	url = {https://m2.mtmt.hu/api/publication/33011261},
	author = {Gohel, Raksha and Rahman, Ashrafur and Lőrincz, Péter and Nagy, Anikó Zsuzsanna and Csordás, Gábor and Zhang, Yan and Juhász, Gábor and Nezis, Ioannis P.},
	doi = {10.1080/15548627.2022.2098765},
	journal-iso = {AUTOPHAGY},
	journal = {AUTOPHAGY},
	volume = {18},
	unique-id = {33011261},
	issn = {1554-8627},
	abstract = {The LIR motif-docking site (LDS) of Atg8/LC3 proteins is essential for the binding of LC3-interacting region (LIR)-containing proteins and their subsequent degradation by macroautophagy/autophagy. In our recent study, we created a mutated LDS site in Atg8a, the Drosophila homolog of Atg8/LC3 and found that LDS mutants accumulate known autophagy substrates and have reduced lifespan. We also conducted quantitative proteomics analyses and identified several proteins that are enriched in the LDS mutants, including Gmap (Golgi microtubule-associated protein). Gmap contains a LIR motif and accumulates in LDS mutants. We showed that Gmap and Atg8a interact in a LIR-LDS dependent manner and that the Golgi size and morphology are altered in Atg8a-LDS and Gmap-LIR motif mutants. Our findings highlight a role for Gmap in the regulation of Golgiphagy.},
	keywords = {DROSOPHILA; GOLGI; Autophagy; LIR motif; Golgiphagy; LIR-motif docking site},
	year = {2022},
	eissn = {1554-8635},
	pages = {2508-2509},
	orcid-numbers = {Lőrincz, Péter/0000-0001-7374-667X; Nagy, Anikó Zsuzsanna/0000-0002-0979-1617; Csordás, Gábor/0000-0001-6871-6839; Juhász, Gábor/0000-0001-8548-8874}
}

@article{MTMT:32876121,
	title = {GMAP is an Atg8a-interacting protein that regulates Golgi turnover in Drosophila},
	url = {https://m2.mtmt.hu/api/publication/32876121},
	author = {Rahman, Ashrafur and Lőrincz, Péter and Gohel, Raksha and Nagy, Anikó Zsuzsanna and Csordás, Gábor and Zhang, Yan and Juhász, Gábor and Nezis, Ioannis P.},
	doi = {10.1016/j.celrep.2022.110903},
	journal-iso = {CELL REP},
	journal = {CELL REPORTS},
	volume = {39},
	unique-id = {32876121},
	issn = {2211-1247},
	year = {2022},
	eissn = {2211-1247},
	orcid-numbers = {Lőrincz, Péter/0000-0001-7374-667X; Nagy, Anikó Zsuzsanna/0000-0002-0979-1617; Csordás, Gábor/0000-0001-6871-6839; Juhász, Gábor/0000-0001-8548-8874}
}

@article{MTMT:32697766,
	title = {The mechanosensor Filamin A/Cheerio promotes tumorigenesis via specific interactions with components of the cell cortex},
	url = {https://m2.mtmt.hu/api/publication/32697766},
	author = {Külshammer, Eva and Kilinc, Merve and Csordás, Gábor and Bresser, Tina and Nolte, Hendrik and Uhlirova, Mirka},
	doi = {10.1111/febs.16408},
	journal-iso = {FEBS J},
	journal = {FEBS JOURNAL},
	volume = {289},
	unique-id = {32697766},
	issn = {1742-464X},
	abstract = {Cancer development has been linked to aberrant sensing and interpretation of mechanical cues and force-generating properties. Here, we show that upregulation of the actin crosslinking protein Cheerio (Cher), the fly ortholog of Filamin A (FLNA), and the conformation of its mechanosensitive region (MSR) are instrumental to the malignancy of polarity-deficient, Ras-driven tumours in Drosophila epithelia. We demonstrate that impaired growth and cytoskeletal contractility of tumours devoid of cher can be rescued by stimulating myosin activity. Profiling the Cher interactome in tumour-bearing imaginal discs identified several components of the cell cortex, including the β-heavy Spectrin Karst (Kst), the scaffolding protein Big bang (Bbg), and 14-3-3ε. We show that Cher binds Bbg through the MSR while the interaction with 14-3-3ε and Kst is MSR-independent. Importantly, our genetic studies define Bbg, Kst, and 14-3-3ε as tumour suppressors. The tumour-promoting function of Cher thus relies on its capacity to control the contractile state of the cytoskeleton through interactions with myosin and specific components of the cell cortex. © 2022 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies},
	year = {2022},
	eissn = {1742-4658},
	pages = {4497-4517},
	orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Uhlirova, Mirka/0000-0002-5735-8287}
}

@article{MTMT:32543729,
	title = {Ion Channels and Pumps in Autophagy: A Reciprocal Relationship},
	url = {https://m2.mtmt.hu/api/publication/32543729},
	author = {Abuammar, Hussein and Bhattacharjee, Arindam and Simon-Vecsei, Zsófia Judit and Blastyák, András and Csordás, Gábor and Páli, Tibor and Juhász, Gábor},
	doi = {10.3390/cells10123537},
	journal-iso = {CELLS-BASEL},
	journal = {CELLS},
	volume = {10},
	unique-id = {32543729},
	year = {2021},
	eissn = {2073-4409},
	orcid-numbers = {Simon-Vecsei, Zsófia Judit/0000-0001-7909-4895; Csordás, Gábor/0000-0001-6871-6839; Páli, Tibor/0000-0003-1649-1097; Juhász, Gábor/0000-0001-8548-8874}
}

@article{MTMT:31743832,
	title = {There and back again: The mechanisms of differentiation and transdifferentiation in Drosophila blood cells},
	url = {https://m2.mtmt.hu/api/publication/31743832},
	author = {Csordás, Gábor and Gábor, Erika and Honti, Viktor},
	doi = {10.1016/j.ydbio.2020.10.006},
	journal-iso = {DEV BIOL},
	journal = {DEVELOPMENTAL BIOLOGY},
	volume = {469},
	unique-id = {31743832},
	issn = {0012-1606},
	year = {2021},
	eissn = {1095-564X},
	pages = {135-143},
	orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839}
}

@article{MTMT:31686151,
	title = {Eater cooperates with Multiplexin to drive the formation of hematopoietic compartments},
	url = {https://m2.mtmt.hu/api/publication/31686151},
	author = {Csordás, Gábor and Grawe, Ferdinand and Uhlirova, Mirka},
	doi = {10.7554/eLife.57297},
	journal-iso = {ELIFE},
	journal = {ELIFE},
	volume = {9},
	unique-id = {31686151},
	issn = {2050-084X},
	abstract = {Blood development in multicellular organisms relies on specific tissue microenvironments that nurture hematopoietic precursors and promote their self-renewal, proliferation, and differentiation. The mechanisms driving blood cell homing and their interactions with hematopoietic microenvironments remain poorly understood. Here, we use the Drosophila melanogaster model to reveal a pivotal role for basement membrane composition in the formation of hematopoietic compartments. We demonstrate that by modulating extracellular matrix components, the fly blood cells known as hemocytes can be relocated to tissue surfaces where they function similarly to their natural hematopoietic environment. We establish that the Collagen XV/XVIII ortholog Multiplexin in the tissue-basement membranes and the phagocytosis receptor Eater on the hemocytes physically interact and are necessary and sufficient to induce immune cell-tissue association. These results highlight the cooperation of Multiplexin and Eater as an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila},
	year = {2020},
	eissn = {2050-084X},
	orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839}
}