TY - JOUR AU - Dernovics, Áron AU - Seprényi, György AU - Rázga, Zsolt AU - Ayaydin, Ferhan AU - Veréb, Zoltán AU - Megyeri, Klára TI - Phenol-Soluble Modulin α3 Stimulates Autophagy in HaCaT Keratinocytes JF - BIOMEDICINES J2 - BIOMEDICINES VL - 11 PY - 2023 IS - 11 PG - 18 SN - 2227-9059 DO - 10.3390/biomedicines11113018 UR - https://m2.mtmt.hu/api/publication/34430365 ID - 34430365 N1 - Department of Medical Microbiology, Albert Szent-Györgyi Medical School, University of Szeged, Dóm tér 10., Szeged, H-6720, Hungary Department of Anatomy, Histology and Embryology, Albert Szent-Györgyi Medical School, University of Szeged, Kossuth L. sgt. 40, Szeged, H-6724, Hungary Department of Pathology, University of Szeged, Állomás u. 2, Szeged, H-6720, Hungary Hungarian Centre of Excellence for Molecular Medicine (HCEMM) Nonprofit Ltd., Római krt. 21., Szeged, H-6723, Hungary Laboratory of Cellular Imaging, Biological Research Centre, Eötvös Loránd Research Network, Temesvári krt. 62., Szeged, H-6726, Hungary Regenerative Medicine and Cellular Pharmacology Laboratory, Department of Dermatology and Allergology, University of Szeged, Korányi Fasor 6, Szeged, H-6720, Hungary University of Szeged, Szeged, Biobank, H-6720, Hungary Interdisciplinary Research Development and Innovation Center of Excellence, University of Szeged, Szeged, H-6720, Hungary Export Date: 12 December 2023 Correspondence Address: Megyeri, K.; Department of Medical Microbiology, Dóm tér 10., Hungary; email: megyeri.klara@med.u-szeged.hu LA - English DB - MTMT ER - TY - CONF AU - Igaz, Nóra AU - Szőke, Krisztina AU - Bocz, Csenge AU - Kovács, Dávid AU - Rónavári, Andrea AU - Szabó, Emilia Rita AU - Polanek, Róbert AU - Buhala, Andrea AU - Vizler, Csaba AU - Tiszlavicz, László AU - Rázga, Zsolt AU - Hideghéty, Katalin AU - Kónya, Zoltán AU - Csontné Kiricsi, Mónika TI - Radiosensitizing effect of metal nanoparticles in combination with histone deacetylase inhibitors T2 - FAMÉ 2023 PY - 2023 SP - 75 EP - 76 PG - 2 UR - https://m2.mtmt.hu/api/publication/34154565 ID - 34154565 LA - English DB - MTMT ER - TY - JOUR AU - Kun-Varga, Anikó AU - Konczné Gubán, Barbara AU - Miklós, Vanda AU - Parvaneh, Shahram AU - Guba, Melinda AU - Szűcs, Diána AU - Monostori, Tamás AU - Varga, János AU - Varga, Ákos AU - Rázga, Zsolt AU - Csörgő Sándorné Bata, Zsuzsanna AU - Kemény, Lajos AU - Megyeri, Klára AU - Veréb, Zoltán TI - Herpes Simplex Virus Infection Alters the Immunological Properties of Adipose-Tissue-Derived Mesenchymal-Stem Cells JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 15 PG - 26 SN - 1661-6596 DO - 10.3390/ijms241511989 UR - https://m2.mtmt.hu/api/publication/34085614 ID - 34085614 N1 - HCEMM-SZTE Skin Research Group, University of Szeged, Hungary, TKP2021-EGA-28 AB - The proper functioning of mesenchymal stem cells (MSCs) is of paramount importance for the homeostasis of the body. Inflammation and infection can alter the function of MSCs, which can also affect the regenerative potential and immunological status of tissues. It is not known whether human herpes simplex viruses 1 and 2 (HSV1 and HSV2), well-known human pathogens that can cause lifelong infections, can induce changes in MSCs. In non-healing ulcers, HSV infection is known to affect deeper tissue layers. In addition, HSV infection can recur after initially successful cell therapies. Our aim was to study the response of adipose-derived MSCs (ADMSCs) to HSV infection in vitro. After confirming the phenotype and differentiation capacity of the isolated cells, we infected the cells in vitro with HSV1-KOS, HSV1-532 and HSV2 virus strains. Twenty-four hours after infection, we examined the gene expression of the cells via RNA-seq and RT-PCR; detected secreted cytokines via protein array; and determined autophagy via Western blot, transmission electron microscopy (TEM) and fluorescence microscopy. Infection with different HSV strains resulted in different gene-expression patterns. In addition to the activation of pathways characteristic of viral infections, distinct non-immunological pathways (autophagy, tissue regeneration and differentiation) were also activated according to analyses with QIAGEN Ingenuity Pathway Analysis, Kyoto Encyclopedia of Genes and Genome and Genome Ontology Enrichment. Viral infections increased autophagy, as confirmed via TEM image analysis, and also increased levels of the microtubule-associated protein light chain 3 (LC3B) II protein. We identified significantly altered accumulation for 16 cytokines involved in tissue regeneration and inflammation. Our studies demonstrated that HSV infection can alter the viability and immunological status of ADMSCs, which may have implications for ADMSC-based cell therapies. Alterations in autophagy can affect numerous processes in MSCs, including the inhibition of tissue regeneration as well as pathological differentiation. LA - English DB - MTMT ER - TY - JOUR AU - Szatmári, Orsolya AU - Nagy-Mikó, Bence AU - Györkei, Ádám AU - Varga, Dániel AU - H. Kovács, Bálint Barna AU - Igaz, Nóra AU - Bognár, Bence AU - Rázga, Zsolt AU - Nagy, Gábor AU - Zsindely, Nóra AU - Bodai, László AU - Papp, Balázs AU - Erdélyi, Miklós AU - Csontné Kiricsi, Mónika AU - Blastyák, András AU - Collart, Martine A AU - Boros, Imre Miklós AU - Villanyi, Zoltan TI - Phase-separated ribosome-nascent chain complexes in genotoxic stress response JF - RNA-A PUBLICATION OF THE RNA SOCIETY J2 - RNA VL - 29 PY - 2023 IS - 10 SP - 1557 EP - 1574 PG - 18 SN - 1355-8382 DO - 10.1261/rna.079755.123 UR - https://m2.mtmt.hu/api/publication/34067381 ID - 34067381 N1 - Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, 6726, Hungary Institute of Biochemistry, Biological Research Centre, Szeged, 6726, Hungary Section for Physiology and Cell Biology, Department of Biosciences, University of Oslo, Oslo, 0316, Norway Department of Optics and Quantum Electronics, University of Szeged, Szeged, 6720, Hungary Department of Pathology, Faculty of Medicine, University of Szeged, Szeged, 6720, Hungary Institute of Genetics, Biological Research Centre, Szeged, 6726, Hungary Department of Microbiology and Molecular Medicine, Institute of Genetics and Genomics Geneva, Faculty of Medicine, University of Geneva, Geneva 4, 1211, Switzerland Cited By :1 Export Date: 12 December 2023 CODEN: RNARF Correspondence Address: Villányi, Z.; Department of Biochemistry and Molecular Biology, Hungary; email: villanyi.zoltan@bio.u-szeged.hu Chemicals/CAS: 1,6 hexanediol, 629-11-8; DNA helicase; edetic acid, 150-43-6, 60-00-4; ribonuclease, 59794-03-5, 9001-99-4; transcriptional regulator ATRX; Werner syndrome ATP dependent helicase; RNA, 63231-63-0; Edetic Acid; RecQ Helicases; Ribonucleoproteins; RNA; Saccharomyces cerevisiae Proteins; SGS1 protein, S cerevisiae Funding details: 31003A_172999, NTP-NFTÖ-20-B-0354 Funding details: Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, SNF Funding details: Magyar Tudományos Akadémia, MTA, BO/00878/19/8, BO/902/19, TKP2021-NVA-19 Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFI Funding text 1: We are grateful to Dr. Balázs Vedelek and Dr. Zsuzsa Sarkadi for valuable discussions. We thank Jawad Iqbal, Elvira Czvik, Zita Kóra, and Edina Pataki for technical assistance. We are grateful to Blanka Léhy for the graphical abstract. This work was supported by grants GINOP-2.3.2-15-2016-00020 and GINOP-2.3.2-15-2016-00038, as well as by NKFI-K 142961 (Z.V.), ÚNKP-21-5-595-SZTE (Z.V.), and ÚNKP-20-5-SZTE-655 (M.K.) from the Hungarian National Research, Development and Innovation Office. Further support was provided by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/902/19 for Z.V. and BO/00878/19/8 for M.K.). Superresolu-tion dSTORM experiments and their evaluation were funded by the Hungarian National Research, Development and Innovation Office (TKP2021-NVA-19), Hungarian Brain Research Program (2017-1.2.1-NKP-2017-00002) awarded to M.E., and NTP-NFTÖ-20-B-0354 awarded to D.V., as well as grant 31003A_172999 from the Swiss National Science Foundation awarded to M.A.C. AB - Assemblysomes are EDTA- and RNase-resistant ribonucleoprotein (RNP) complexes of paused ribosomes with protruding nascent polypeptide chains. They have been described in yeast and human cells for the proteasome subunit Rpt1, and the disordered N-terminal part of the nascent chain was found to be indispensable for the accumulation of the Rpt1-RNP into assemblysomes. Motivated by this, to find other assemblysome-associated RNPs we used bioinformatics to rank subunits of Saccharomyces cerevisiae protein complexes according to their N-terminal disorder propensity. The results revealed that gene products involved in DNA repair are enriched among the top candidates. The Sgs1 DNA helicase was chosen for experimental validation. We found that indeed nascent chains of Sgs1 form EDTA-resistant RNP condensates, assemblysomes by definition. Moreover, upon exposure to UV, SGS1 mRNA shifted from assemblysomes to polysomes, suggesting that external stimuli are regulators of assemblysome dynamics. We extended our studies to human cell lines. The BLM helicase, ortholog of yeast Sgs1, was identified upon sequencing assemblysome-associated RNAs from the MCF7 human breast cancer cell line, and mRNAs encoding DNA repair proteins were overall enriched. Using the radiation-resistant A549 cell line, we observed by transmission electron microscopy that 1,6-hexanediol, an agent known to disrupt phase-separated condensates, depletes ring ribosome structures compatible with assemblysomes from the cytoplasm of cells and makes the cells more sensitive to X-ray treatment. Taken together these findings suggest that assemblysomes may be a component of the DNA damage response from yeast to human. LA - English DB - MTMT ER - TY - CONF AU - Madácsy, Tamara AU - Varga, Árpád AU - Csákány-Papp, Noémi AU - Tél, Bálint AU - Pallagi, Petra AU - Szabó, Viktória AU - Kiss, Aletta Kata AU - Fanczal, Júlia AU - Rakonczay, Zoltán AU - Tiszlavicz, László AU - Rázga, Zsolt AU - Hohweiler, Meike AU - Kleger, Alexander AU - Gray, Mike AU - Hegyi, Péter AU - Maléth, József TI - Defective CFTR expression promotes epithelial cell damage in alcoholic pancreatitis and hepatitis by the impaired regulation of PMCA activity T2 - European Cystic Fibrosis Society 17th ECFS Basic Science Conference C1 - Albufeira PY - 2022 SP - 135 UR - https://m2.mtmt.hu/api/publication/34657419 ID - 34657419 LA - English DB - MTMT ER - TY - JOUR AU - Konczné Gubán, Barbara AU - Flink, Lili Borbála AU - Bozó, Renáta AU - Danis, Judit AU - Rázga, Zsolt AU - Koncz, Balázs AU - Széll, Márta AU - Kemény, Lajos AU - Csörgő Sándorné Bata, Zsuzsanna TI - Abnormal type VII collagen expression in non-lesional psoriatic skin JF - JOURNAL OF INVESTIGATIVE DERMATOLOGY J2 - J INVEST DERMATOL VL - 142 PY - 2022 IS - Suppl. 12 SP - S244 EP - S244 PG - 1 SN - 0022-202X DO - 10.1016/j.jid.2022.09.386 UR - https://m2.mtmt.hu/api/publication/33261227 ID - 33261227 LA - English DB - MTMT ER - TY - JOUR AU - Konczné Gubán, Barbara AU - Flink, Lili Borbála AU - Bozó, Renáta AU - Danis, Judit AU - Rázga, Zsolt AU - Koncz, Balázs AU - Széll, Márta AU - Kemény, Lajos AU - Csörgő Sándorné Bata, Zsuzsanna TI - Abnormal type VII collagen expression in non-lesional psoriatic skin JF - BŐRGYÓGYÁSZATI ÉS VENEROLÓGIAI SZEMLE J2 - BVSZ VL - 98 PY - 2022 IS - 4 SP - 190 EP - 190 PG - 1 SN - 0006-7768 UR - https://m2.mtmt.hu/api/publication/33190130 ID - 33190130 LA - English DB - MTMT ER - TY - JOUR AU - Madácsy, Tamara AU - Varga, Árpád AU - Csákány-Papp, Noémi AU - Tél, Bálint AU - Pallagi, Petra AU - Szabó, Viktória AU - Kiss, Aletta Kata AU - Fanczal, Júlia AU - Rakonczay, Zoltán AU - Tiszlavicz, László AU - Rázga, Zsolt AU - Hohwieler, Meike AU - Kleger, Alexander AU - Gray, Mike AU - Hegyi, Péter AU - Maléth, József TI - Impaired regulation of PMCA activity by defective CFTR expression promotes epithelial cell damage in alcoholic pancreatitis and hepatitis. JF - CELLULAR AND MOLECULAR LIFE SCIENCES J2 - CELL MOL LIFE SCI VL - 79 PY - 2022 IS - 5 PG - 18 SN - 1420-682X DO - 10.1007/s00018-022-04287-1 UR - https://m2.mtmt.hu/api/publication/32800045 ID - 32800045 N1 - Department of Medicine, University of Szeged, Szeged, 6720, Hungary HAS-USZ Momentum Epithelial Cell Signaling and Secretion Research Group, University of Szeged, Szeged, 6720, Hungary First Department of Pediatrics, Semmelweis University, Budapest, Hungary Department of Pathophysiology, University of Szeged, Szeged, 6720, Hungary Department of Pathology, University of Szeged, Szeged, Hungary Department of Internal Medicine I, Ulm University Hospital, Ulm, Germany Biosciences Institute, Newcastle University, Newcastle upon Tyne, United Kingdom Institute for Translational Medicine, University of Pécs, Pécs, Hungary Centre for Translational Medicine and Division for Pancreatic Disorders, Semmelweis University, Budapest, Hungary HCEMM-USZ Molecular Gastroenterology Research Group, University of Szeged, Szeged, 6720, Hungary Cited By :4 Export Date: 26 January 2024 CODEN: CMLSF Correspondence Address: Maléth, J.; Department of Medicine, Hungary; email: jozsefmaleth1@gmail.com AB - Alcoholic pancreatitis and hepatitis are frequent, potentially lethal diseases with limited treatment options. Our previous study reported that the expression of CFTR Cl- channel is impaired by ethanol in pancreatic ductal cells leading to more severe alcohol-induced pancreatitis. In addition to determining epithelial ion secretion, CFTR has multiple interactions with other proteins, which may influence intracellular Ca2+ signaling. Thus, we aimed to investigate the impact of ethanol-mediated CFTR damage on intracellular Ca2+ homeostasis in pancreatic ductal epithelial cells and cholangiocytes. Human and mouse pancreas and liver samples and organoids were used to study ion secretion, intracellular signaling, protein expression and interaction. The effect of PMCA4 inhibition was analyzed in a mouse model of alcohol-induced pancreatitis. The decreased CFTR expression impaired PMCA function and resulted in sustained intracellular Ca2+ elevation in ethanol-treated and mouse and human pancreatic organoids. Liver samples derived from alcoholic hepatitis patients and ethanol-treated mouse liver organoids showed decreased CFTR expression and function, and impaired PMCA4 activity. PMCA4 co-localizes and physically interacts with CFTR on the apical membrane of polarized epithelial cells, where CFTR-dependent calmodulin recruitment determines PMCA4 activity. The sustained intracellular Ca2+ elevation in the absence of CFTR inhibited mitochondrial function and was accompanied with increased apoptosis in pancreatic epithelial cells and PMCA4 inhibition increased the severity of alcohol-induced AP in mice. Our results suggest that improving Ca2+ extrusion in epithelial cells may be a potential novel therapeutic approach to protect the exocrine pancreatic function in alcoholic pancreatitis and prevent the development of cholestasis in alcoholic hepatitis. LA - English DB - MTMT ER - TY - GEN AU - Szatmári, Orsolya AU - Györkei, Á AU - Varga, Dániel AU - H. Kovács, Bálint Barna AU - Igaz, Nóra AU - Német, K AU - Bagi, N AU - Nagy-Mikó, B AU - Balogh, D AU - Rázga, Zsolt AU - Erdélyi, Miklós AU - Papp, B AU - Csontné Kiricsi, Mónika AU - Blastyák, A AU - Collart, MA AU - Boros, Imre Miklós AU - Villanyi, Zoltan TI - Phase separated ribosome nascent chain complexes paused in translation are capable to continue expression of proteins playing role in genotoxic stress response upon DNA damage PY - 2022 DO - 10.1101/2022.03.16.484567 UR - https://m2.mtmt.hu/api/publication/32783378 ID - 32783378 LA - English DB - MTMT ER - TY - JOUR AU - Virók, Dezső AU - Tömösi, Ferenc AU - Keller-Pintér, Anikó AU - Szabó, Kitti AU - Bogdanov, Anita AU - Póliska, Szilárd AU - Rázga, Zsolt AU - Bruszel, Bella AU - Cseh, Zsuzsanna AU - Kókai, Dávid AU - Paróczai, Dóra AU - Endrész, Valéria AU - Janáky, Tamás AU - Burián, Katalin TI - Indoleamine 2,3-Dioxygenase Cannot Inhibit Chlamydia trachomatis Growth in HL-60 Human Neutrophil Granulocytes JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 12 PY - 2021 PG - 14 SN - 1664-3224 DO - 10.3389/fimmu.2021.717311 UR - https://m2.mtmt.hu/api/publication/32489937 ID - 32489937 N1 - FUNDING: DV, TJ, FT, and BB were supported by EFOP 3.6.1 Program. TJ, FT, and BB were supported by the Thematic Excellence Program 2020 (TKP2020-IKA-07). LA - English DB - MTMT ER -